Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,878,9470CT25.6% 72.8 / 10.1 44E276K (GAA→AAA) cas1multifunctional nuclease Cas1
Reads supporting (aligned to +/- strand):  ref base C (7/25);  new base T (11/0);  total (19/25)
Fisher's exact test for biased strand distribution p-value = 5.53e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAGTCCAGCTATTTGTTCCCAGATCATTTCACGAATTTTTGCGGATACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTG‑T‑ATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGG‑AATCAA‑TTTGGCTAATGTTTTACTACTGCGAAAAATATCCCTGCAC‑GCCAAACGG‑ACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAATATCGTAAACAAAGGAAAGAGGCTTTCCTGTATGCACAAACCCAATAGCTGGTGCATA  >  NC_000913/2878704‑2879175
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                        
cAGTCCAGCTATTTGGTCCCAGATCATTTCACGAATTTTTGCGGATACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAGCAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTG‑T‑ATTGCTCCAGCg                                                                                                                                                                                                                                   <  2:47645/251‑1 (MQ=255)
            ttGTTCCCAGATCATTTCACGAATTGTTGCGGATACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTGCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCAGCTTCAGGTGGGGCCGGCGGTTG‑T‑ATTTCTCCAGCGGCAAGCACGTc                                                                                                                                                                                                                        <  2:106737/250‑1 (MQ=255)
                tCCCAGATCATTTCACGAATTTTTGCGGATACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTG‑T‑ATTTCTCCAGCGGCAAGCACGTCctct                                                                                                                                                                                                                    <  1:241964/250‑1 (MQ=255)
                           ttCACGAATTTTCAAGGACACACCACCTACATATACCACTGCACGCACCTCCACCAACCAGATGGCTACTCTGCCTCGTAAGAGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGCCCGGCGGTTT‑T‑TTTTTTCCAGCGGCCAGCACgctcatactaaacgcact                                                                                                                                                                                                         >  2:626799/1‑233 (MQ=255)
                              aCGAATTTTTGCGGATACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTG‑T‑ATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGG‑AATCAA‑t                                                                                                                                                                                                   <  1:332794/251‑1 (MQ=255)
                                    ttttGCGGATACATCACCTACATATACCCCTGCACGTACCTCCAACACCCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGAACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGCAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCCGGTGGGGCCGGCGGTTG‑G‑GTTTTTCCCGAGGCCAGAACGCCCCCTATTAGCGG‑GATCAA‑ttttggc                                                                                                                                                                                             >  1:355937/1‑247 (MQ=255)
                                          ggATACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGCTG‑T‑TTTTTTCCTGCCGCCAGCACCTCCTCtatacggcgagtctatatggttactgtt                                                                                                                                                                                         >  2:334258/1‑227 (MQ=255)
                                           gATACATCACCTACATCTACCCCTGCACGTACCTCCAACAACCAGATGGCGAATCTGCCTCGTAGGCGCGGAGGTACATTTTCAGTGACCCCGACCAACATACTCATTTCAGCTACTCCGATGGCCGGCAGCTCCCAGTGAAACAGGAAGCGGAAGGGCAACAGTCTGTGCCTCCGCAGGTGGGTCCGGCGGTGG‑T‑ATTTCTCCAGCGTCGAGCTCTTCCTCGATAAGCGG‑AATCAA‑TTGGGCTAATGttt                                                                                                                                                                                      <  2:415398/251‑1 (MQ=255)
                                            aTACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTG‑T‑ATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGG‑AATCAA‑TTTGGCTAATGtttt                                                                                                                                                                                     <  1:513945/251‑1 (MQ=255)
                                              aCATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTG‑T‑ATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGG‑AATCAA‑TTTGGCTAATGTTTtac                                                                                                                                                                                   <  2:135980/251‑1 (MQ=255)
                                               cATCACCTACATATCCCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTACGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTG‑T‑ATTTCTCCACCGGCAAGCACCTCCTCTCTAAGACG‑AAACAA‑TATGTCTAATGTTTtact                                                                                                                                                                                  >  2:307656/1‑251 (MQ=255)
                                                   aCCTACAGATTCCCCTGCACGTACCCCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACAGACTCATGTCAGCTCCTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGTCCGGCTGTTG‑T‑ATTTCTCCAGCGGCAATCCTGTCCTCTATAAGCGG‑AATCAA‑TTTGGCTAATGTTATACTACTg                                                                                                                                                                              <  1:425240/251‑1 (MQ=255)
                                                       aCATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGTTTG‑T‑ATTTCTCCAGCGGc                                                                                                                                                                                                                                 <  2:429029/198‑1 (MQ=255)
                                                           ataCCCCTGCGCGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTG‑T‑ATTTCTCCAGCGGCAAGCACGTCCGCTATAAGCGG‑AATCAA‑TTTGGCTAATGTTTCACTACTGCTAAAAat                                                                                                                                                                      <  2:105693/251‑1 (MQ=255)
                                                                ccTGCACGTCCCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTG‑T‑ATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGG‑AATCAA‑TTTGGCTAATGTTTTACTACTGCGAAAAATATccc                                                                                                                                                                 <  1:3598/251‑1 (MQ=255)
                                                                            tCCAACAACCAGCTGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTG‑T‑ATTTTTCCAGCGGCAAGCACGTCCTCTATAAGCGG‑AATCAA‑TTTGGCTAATGTTTTTCTACTTCGAAAAATATACCTGCAC‑GCCCAAc                                                                                                                                                    >  1:515404/1‑251 (MQ=255)
                                                                            cccAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACGCAGTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTG‑T‑ATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCTG‑AATCAA‑TTTGGCTAATGTTTTACTACTGCGAAAAATATCCCTGCAC‑GCCAAAc                                                                                                                                                    <  2:299453/250‑1 (MQ=255)
                                                                               aacaacCCGATGGCTAATCTGCCTCGTAAGCTCGGAGGTACATTTTCAGTGACCACGACCAACATACTCACTGCAGCGACGCCTATTGCCTGCATCTCCCAGTTAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGTTGCCAGCGGTTG‑T‑ATATCTCCAGCCTCGAGCACGTCCGCTAGAAACGG‑AATCAA‑TTTGGCTAATGTTGTACCACTGTGAATAAGACCCCTGCGC‑TCCAAACGG‑a                                                                                                                                                <  2:428822/251‑1 (MQ=255)
                                                                               aacaacCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTG‑T‑ATTTTTCCAGCGGCAAGCCCGTCCTCTATTAACGG‑AAACAA‑TTTGGCTAATGTTTTTCTTCTTCGAAAAAAATCCCTTCAC‑CCCCAACggc                                                                                                                                                 >  1:290384/1‑250 (MQ=255)
                                                                                  aacCAGCTGGATAATCTGCCTCGTAAGCGCGGCGGTACATTTTCAGTGACCACGACCAACCTACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTG‑G‑ATTTTTCCAACGGGAAGCACGTCCTCTATTAGAGGTGTTCAA‑TT‑GGCTATTGTTTTTCTATAGCGAAAAataatcccgcgcgccacacagacat                                                                                                                                               >  2:295243/1‑229 (MQ=255)
                                                                                                          aaGCACGGAGGTACATTTTCAGTGACCACCACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTCTGCATCTTCAGGTGGGGCCGGAGGTTG‑T‑ATTTCTCTACCGGCAAGCAAGTCCCCTATTAAAGG‑CATCAA‑ATTTTCTAATTTTTTTTTACTTCGACAAATATTCATCCAC‑GCCCAACGGGCCTTTCCG‑TCCGGCTCACCCGGGttct                                                                                                                     >  1:293345/1‑249 (MQ=255)
                                                                                                           aGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTACCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTG‑T‑TTTTTTCCAGCCGCAAGCACCTCCTCTCTAAACGG‑AAACAA‑TTTGGCTAAAGTTTTACTACTGCGAAAAAAATCCCTGCAC‑GCCCAACGG‑ACTTCCCCGTCCGGCTCACCCGGGTTacg                                                                                                                    >  1:352466/1‑251 (MQ=255)
                                                                                                             cgcgGAGGTACGTTTTCAGTGACCACGACCAACAGACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTGCAGGTGGGGCCGGCGGTTG‑T‑ATTTCTCCAGCGGCAAGCGCTTCCTCTATAAGCGG‑AATCAA‑TTTGGCTAATGTTTTACTACTGCGAAAAAGATCCCTGCAC‑GCCAAACGG‑ACTTCCGGGTCCGGCTCACCAGGGTTacgac                                                                                                                  <  2:541423/251‑1 (MQ=255)
                                                                                                                     tACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTT‑T‑TTTTTTCCCGCGGCAAGCACGTCCTCCATAAGCGG‑AATCAA‑TTTTGCTAATGTTTTTCTACTGCGAAAAATATCCCTGCAC‑GCCAAACGG‑ACGTCCCCGTCCCGCTCACCAGGGGTACGAcgccccatc                                                                                                          >  1:561606/1‑245 (MQ=255)
                                                                                                                       cATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTG‑T‑ATTTCTTCAGCGGCCAGCAAGTCCTCTATAAGCGG‑GATCAA‑TTTTGCTTATGGTTTTCTACTTCGAGAAATATCCCTGCAA‑CCCCAACGG‑ACTTCCCGGTTCGGCTCCCCAGGGGTacgaaccgccatccc                                                                                                        >  2:467195/1‑240 (MQ=255)
                                                                                                                               gTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCACAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTG‑T‑ATTTTTCCAGCGGCAAGCCCGTTCTCTATAAACGG‑GATCAAATTTGG‑TAATGTTTTACTACTGCGAAAAATATCCCTTCAC‑GCCAAACCG‑ACTTCCCGGGCCCGCTCCCCACGGGTACCGCGAGCTATCTCAAAAGCtt                                                                                                >  1:88416/1‑251 (MQ=255)
                                                                                                                                           aaCATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCCTCTTCCGGTGGGGCCGGCGGTTG‑TTATTT‑TCCCGCGGCAAGCACGGCCTCTATCAGCGG‑GAACAA‑TTTGGGTCATGTTTTTCTACTTCGCAAAATATCCCCGCAC‑GCCAAACGG‑ACTTCCCGGCCCCGCTCACCCCGGGTACGACCCGCCATTTCCAAAGCtgtctgggcaac                                                                                      >  2:465408/1‑238 (MQ=255)
                                                                                                                                           aaCATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTG‑T‑ATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGG‑AATCAA‑TTTGGCTAATGTTTTACTACTGCGAAAAATATCCCTGCAC‑GCCAAACGG‑ACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAg                                                                                    <  1:275928/251‑1 (MQ=255)
                                                                                                                                                            tACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTG‑T‑ATTTCCCCAACGGCAAGCACGTCCTCTATAAGGGG‑AATCAA‑TTTGGCTAATGTTTTACTACTGCGAAAAAAAACCCTGCAC‑CCCAAACGG‑ACTTCCCGGGCCGGCTCACCAGGGGTACCACGCGCTATCTCAAAAACTTTTGGTACAAACGGGTCAAAttttaagatt                                                                   >  1:553212/1‑244 (MQ=255)
                                                                                                                                                                ccGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTG‑T‑ATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGG‑AATCAA‑TTTGGCTAATGTTTTACTACTGCGAAAAATATCCCTGCAC‑GCCAAACGG‑ACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTg                                                               <  1:123980/251‑1 (MQ=255)
                                                                                                                                                                 cGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTG‑T‑ATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGG‑AATCAA‑TTTGGCTAATGTTTTACTACTGCGAAAAATATCCCTGCAC‑GCCAAACGG‑ACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGc                                                              <  1:468844/251‑1 (MQ=255)
                                                                                                                                                                       ccagCATCTACCATTGAAACAGGAAGCGGAATTGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTG‑T‑ATTTCTCCAGCGGCAATCACGTCCTCTATAAGCGG‑AATCAA‑TTTGGCTAATGTTTTACTTCTGCGAAAAATATCCCTGCAC‑GCCAAACGG‑ACTTCCCTGTCCGGCTCACCAGGGTTACGACGCTCTATCTCACAATCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTTCAATATc                                                        <  2:70974/248‑1 (MQ=255)
                                                                                                                                                                       ccTGCATCTCCCAGGGAAACAGGAATCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGTGCCGGCGGTTG‑T‑ATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGG‑AATCAA‑TTTGGCTAATGTTTTACTACTGCGAAAAATATCCCTGCAC‑GCCAAACGG‑ACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTGTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAATATc                                                        <  2:202790/251‑1 (MQ=255)
                                                                                                                                                                            aTCTCCCAGTGAAACAGGCAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGCTT‑T‑ATTTTTCCCACGGCAAGCAAGTCCTCTATAAACCG‑AATCAA‑TTTTGCTAATGTTTTTCTCCTGCGACAAAAATCCCTGCAC‑GCCAAACAG‑GCTTCCCTGTCCGGATCACCAGGGTTACGACGCGCTCTTTCCAAAGCTTTTTGTACAACAATGTCAAAATTTatgatttttgccataaccata                                                    >  2:578713/1‑234 (MQ=255)
                                                                                                                                                                                cccAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTG‑T‑ATTTCTCCCACGGCCAACACCTCCTCTATAAACGG‑AATCAA‑TTTTGGTTATGGTTTTCTACTGCCACAAATATCCCTTCAC‑GCCAAACGG‑ACTTTCCCGTCCGGCCCACCCCGGGTACGAAGCGCTATCTCAAAAACTTTCGGTGCCACAGTGTCAAATTTTATTATTTCTGCAataatgtaaaaaa                                                >  2:334912/1‑240 (MQ=255)
                                                                                                                                                                                    gTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTG‑T‑ATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGG‑AATCAA‑TTTGGCTAATGTTTTACTACTGCGAAAAATATCCCTGCAC‑GCCAAACGG‑ACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAgt                                                                                   <  1:128163/211‑1 (MQ=255)
                                                                                                                                                                                        aaCAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGGGGGGCCGGCGGTTG‑T‑ATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGG‑AATCAA‑TTTGGCTAATGTTTTACTACTGCGAAAAATATCCCTGCAC‑GCCAAACGG‑ACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAATAGCGTAAACAAAGGAAagag                                       <  1:172685/251‑1 (MQ=255)
                                                                                                                                                                                                   gAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTG‑T‑ATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGG‑AATCAA‑TTTGGCTAATGTTTTACTACTGCGAAAAATATCCCTGCAC‑GCCAAACGG‑ACTTCCCGGGCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAATCTTTCGGTACAACAGTGTCAAAt                                                                            <  2:428383/203‑1 (MQ=255)
                                                                                                                                                                                                   gAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGATG‑T‑TTTTCTCCAGCGGGAAGCACGTCCCCTCTAAGAGG‑AATATA‑TTTTGGTTATATTTTACTCCTGCGAAAAATATCCATG‑ACAGCCAAACAG‑ACTTCCCGGTCCCGCTCACCAGGGTTACGACGCGCTATCTCCAAAAATTTTGGTACAACAATGTCaaaa                                                                            >  1:428383/1‑202 (MQ=255)
                                                                                                                                                                                                                cTGTGCATCTTCAGGTGGGGCCGGCGGTTGGT‑TTTCCTCCACGGGCAGCCAC‑TCCTCCATAAGCGG‑GAACAA‑TTTTGATAATGGTTTACTACTGCGAAAAAAAACCCTGCAC‑GCCAAACGG‑ACTTCCCGGGCCCGGTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTTGGTACCACAGTGTCAAATTTAATGATGTCTGCACAATCGCAAACAAAGGGAAAAGGCTTTTCTGTATGCACAAACCCa                >  2:233910/1‑250 (MQ=255)
                                                                                                                                                                                                                 tgtgCATCTTCAGGTGGGGCCGGCGGTTG‑T‑ATTTTTCCAGCGGCAAGCACGTCCTCTATAAGCGG‑GATCAA‑ATTGGCTCATGTTTTTCTACTCCGGAAAAAATCCCCGCAC‑GCCAAACGG‑ACTTTCCGGGCCCGCCCACCACGGGTACGACGCGCTATCTCAAAAACTTTCGGTACAACAGAGGCAAATTTAAAGATGTTTGCAATATCGTAAAAAAAAGAAAAAGGGTTTCCTGTATGCACAAAACCAAt              >  1:405182/1‑251 (MQ=255)
                                                                                                                                                                                                                          tCAGGTGGGGCCGGCGGTTG‑T‑ATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGG‑AATCAA‑TTTGGCTAATGTTTTACTACTGCGAAAAATATCCCTGCAC‑GCCAAACGG‑ACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAATATCGTAAACAAAGGAAAGAGGCTTTCCTGTATGCACAAACCCAATAGCTGGTg      <  2:515404/250‑1 (MQ=255)
                                                                                                                                                                                                                          tCAGGTGGGGCCGGCGGTTG‑T‑ATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGG‑AATCAA‑TTTGGCTAATGTTTTACGACTGCGAAAAATATCCCTGCAC‑GCCAAACGG‑ACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAATATCGTAAACAAAGGAAAGAGGCTTTCCTGTATGCACAAACCCAATAGCTGGTg      <  2:290384/250‑1 (MQ=255)
                                                                                                                                                                                                                             ggTGGGGCCGGCGGTTG‑T‑ATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGG‑AATCAA‑TTTGGCTAATGTTTTACTACTGCGAAAAATATCCCTGCAC‑GCCAAACGG‑ACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAATATCGTAAACAAAGGAAAGAGGCTTTCCTGTATGCACAAACCCAATAGCTGGTGCATa  <  1:490837/251‑1 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                        
CAGTCCAGCTATTTGTTCCCAGATCATTTCACGAATTTTTGCGGATACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTG‑T‑ATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGG‑AATCAA‑TTTGGCTAATGTTTTACTACTGCGAAAAATATCCCTGCAC‑GCCAAACGG‑ACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAATATCGTAAACAAAGGAAAGAGGCTTTCCTGTATGCACAAACCCAATAGCTGGTGCATA  >  NC_000913/2878704‑2879175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑