| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 811,721 | 0 | T | G | 32.9% | 100.0 / 10.6 | 74 | V67G (GTG→GGG) | bioC | malonyl‑acyl carrier protein methyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (7/42); new base G (20/4); total (28/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.26e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCG‑CCCCAAAGGCGTGGTCGCGTTTACCACGCTGGTGCAGGGATCGTTACCCGAACT > NC_000913/811477‑811967 | cTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGGACGTCACGCGCAGGGGACGGc > 1:73807/1‑251 (MQ=255) cgcATGAAAGGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCtt < 1:131620/251‑1 (MQ=255) aaTGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACCCTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGGCACGCGGAGGGGACGGGCCTAGAtccc > 1:417755/1‑249 (MQ=255) aaTGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGAtctc < 1:99323/251‑1 (MQ=255) aaTGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGAtctc > 1:193806/1‑251 (MQ=255) aaTGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACGGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGAtctc < 1:644465/251‑1 (MQ=255) aTGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGAtctc‑t < 1:286497/251‑1 (MQ=255) gCAGGTTATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑Tcg < 1:25778/251‑1 (MQ=255) aGGATATCGCCCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCAGTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑Tcgcc < 1:506479/251‑1 (MQ=255) aGGATACCGACCGTCTGCGGGAGGGTCTGCAGGGAAACGGTGAATAAATAAGCCATTGCAGCGGTATTTGGTCGGGCAGCCGCCCACGATGAGCAACATGCAGATCTACAGCGCCAGAGGGTTGACGCCTTACTGGCTATGCTTCCACATCGTAAATACCCCCCTGTTCTGTATGCGTGGTGTGG‑ACCGGGCTGGAGGAGCCGCCACTGTCGTGATCTTCACGCTCAGGTGACGGCCTTGGATCTC‑Tcgcc < 2:596891/251‑1 (MQ=255) gATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAAAAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑Tcgccgc < 1:580358/251‑1 (MQ=255) atCGACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGGACGTCCCGCGCAGGGGGCGGGCCTAGATCTC‑TCgccgccc > 1:270183/1‑249 (MQ=255) atCGACCGTCTGCTGGAGGTGCGGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCGACAGCGCCAGAGTGCGTACGCCTTACTGGCAATGCTTCCACAGCGTAAATCCCCCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGTATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCaa < 1:633023/251‑1 (MQ=255) gACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTATATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCAATGc < 1:458400/251‑1 (MQ=255) cGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCAATGCTTg < 1:377508/251‑1 (MQ=255) cGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGGAGGCCCTAGATCTC‑TCGCCGCCAATGCTTg > 1:396691/1‑251 (MQ=255) ggAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCCCGCGCAGGGGACGGCCTTAGATCTC‑TCGCCGCCCATGCTTGTTCAGGcc > 1:189541/1‑250 (MQ=255) gAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAAAGTCACGCGCAGGGGAAGGCCCTAGATCTC‑TCGCCGCCAATGCCTGTTccagcac > 1:39722/1‑245 (MQ=255) gAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACAGGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCAc < 1:86591/251‑1 (MQ=255) gccacagtgaacctgtaaataaACAAGCCATTGCAGGTGCTTTTGGTCGGGCAGCCGCGCACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCTTTTCTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTTGACGCGGGTTGTGG‑ACCTGGCTGTATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑GCGCCGCCAAGGCGTGGTCAGGCACGCCa < 2:439134/233‑1 (MQ=255) gcATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCAGaa < 1:532904/250‑1 (MQ=255) gggCAACGTTAAAAAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACGGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGAt < 2:73807/250‑1 (MQ=255) gCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGc < 1:266315/251‑1 (MQ=255) aaCGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGGACGTCACGCGCAGGGGGCGGGCCTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCAGGAGGATGCCg > 1:128228/1‑251 (MQ=255) gggtaaaaaaccAAGCCAGTGCAGCGGAATGTGGCCGGGCAGCCGCACACTATGGGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAAGGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTGGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGAGCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCa < 2:561543/240‑1 (MQ=255) cGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATACAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAAAGGCACGCGCAGGTGGCGGCCTTAGAT‑TCATCGCCGCCAAAGCTTGTTTAGGCACGCCAGCAGGATGACGcc > 1:498135/1‑250 (MQ=255) gTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGGACGTCCCGCGCAGGGGACGGCCTTAGATCTC‑TCGCCGCCCAAGCTTGTTCAGGCACCCCCGAAAGGTGCCGccg > 1:247528/1‑248 (MQ=255) aataaACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGGACGTCACGCGCCGGGGACGGCCTTAGAAATC‑TCCCCGCCCACGCCTGTTCCGGGACGCCAGAAGGAAGCCGCAgcac > 1:376357/1‑247 (MQ=255) aaaaaaCAAGCCATTGCAGCGGCATTGGGTCGGGCGGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCa < 2:546035/248‑1 (MQ=255) aaaCAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCCTAGATCTC‑TCGCCGCCAATGATTGTTCACGCACGCCCGAAAGATGCCGCAGACCAttt > 1:487555/1‑250 (MQ=255) aCAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATc < 1:244331/251‑1 (MQ=255) cAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCt < 1:345711/251‑1 (MQ=255) aaGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCGACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑TGGCCGCCGATGCGTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTg < 1:332908/251‑1 (MQ=255) ccATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACCCCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCg < 2:270183/251‑1 (MQ=255) ttGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGACACACCCAGGTGAAGGACCTACATCTC‑TCTCCCCCCATGCTTGTTCAGGCACGCCCGAAGGATGACGCCAACCCTTATCTGGCgggg > 1:652977/1‑250 (MQ=255) aTTTGGTCGGGCAGCAGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGGACGCCACCCGCCGGGGGCGGCCCTAGATACC‑TCCCCGCCCATGCTTGTTCAGGCCCGCCCGAAGGAGGCCGCAGACCACTTTCTTGCGGgaggattccaa > 2:284955/1‑242 (MQ=255) tttggccgggccaccgaacgctaggAGCAACAGGCAATTCTACACCGCCAGGGGTCTGACGCCTTACTGGCAAGGCTTCCACAGCTGAAAGACACCCTCGTCCGGCACGCGGGTTTTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCATACCAGGATCTGGCGGGACATATCGAAt < 2:280906/226‑1 (MQ=255) gtgggccggccagccccctacTATGAGCAACATGCAGATCTACAGCGCGAGAGGGCGGACGCCTTCCGGGCAATGTTTCCCCAGGGAAAACCCCCCCCCGGAGTGGAGGCGGGT‑GTGGGACCTGTCTGGAGGGGCCGCCTCTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGTACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAAt < 2:464603/231‑1 (MQ=255) ggTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATccc < 1:198753/250‑1 (MQ=255) ggTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCCCAGGGGAAGGCCCTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCACAAAGAGGCCGCAGACCATTATTTTGCGGGGGGTATCGAATTCCt > 1:565388/1‑251 (MQ=255) gTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTg < 1:129800/251‑1 (MQ=255) gCCGCACACTATGAGCAACATGCATATCTACAGCGCCAGAGTGCGGACGCCTTACTGGCAATGTTTCCACGGCGTAAATCCCCCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACTTCACGCGCAGGGGACGGCCTTAGATCTT‑TCGCGGCCAATGCTTGTTCAGGCACGCCAGAATGATGCCTCAGTCCATTATCGGGCGGGAGATATCGAATCCCTGCCGTTAGc < 1:73590/251‑1 (MQ=255) gCACACTATGAGCAACATGCAGATCTACAGCGCCAGCGAGCTGCCGCCTTACTGGCAATGCTTCCACAGCGTAAATACCCCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGGAAGTCACGCGGAGGGGAAGGCCCTAGATCTT‑TCGACGCCCATGCTCGTTCCGGGACGCCAGAAGGATGCCGCAGGCCAATATCTGGCGGGGGAGATCGGATCCCTGccctttgagac > 1:251995/1‑242 (MQ=255) ggaaacttGCAGATCTACGGCGCCGGGGTGCTGCCCCCTTCGTGGAAATGTTTCCCCAGCGAAAATACCCCCACGTCCGGGCCGGGGTTTGTGG‑CCCGGGCTGGAGGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTc < 2:193806/244‑1 (MQ=255) aaaacatgcatacctacgcccccAGAGGGCGGACGCCTTACTGGCAATTCTTCCACAGCTTAAATACCCCCACGTACGGGAGGCGGGTTGGGG‑CCGGGGCTGAATGGGCCGCCACGGGCGGGAACGTCACGCGCAGGGGACGTCCTTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGAGGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCg < 2:396691/230‑1 (MQ=255) acaggCAGATCGACAGCGCAAGAGTGCTGCCGCCTTACTGGCAGTGCTCCCACGGCGAAAACCCCCCCCCGTCGGGGACGCGGGTGGTGG‑ACCGGGCTGGAGGGGCCGCCACGGGCGGGAACGTCACGCGCAGGTGACGGCCTGAGATCTC‑GCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCGTTATCTGGCGGGAGATATGAAATCCCTGCCGTTAGCGACTGCGACGTTCGATc < 2:608975/247‑1 (MQ=255) aCATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTACACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGGCACGCGCCGGGGGCGGGCTTAGAACTC‑TCGCCCCCCATGCTTTTTCCGGCACGCCCCAAGGGAGCCGCCGACCCCTATCTGGCGGGGGGTATCGCATCCccgccggttgacactgacacgttcgatt > 2:49090/1‑229 (MQ=255) cATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGGAGGGGAAGGCCCTAGGTCCC‑TCGCCGCCCATGCTTGTTCAGGCACCCCAGAAGGAAGCCGCCGACCATTATTTGTCGGGGGGTATCGAATCGCTGCCGGTAGCGAccgctaaggtcggtct > 1:94414/1‑236 (MQ=255) cAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCCCGCGCAGGGGACGGCCTTAGATCTC‑TCCCCCCCAATGCTTGTTCAGGCCCGCCCGACGGATGCCGCAGACCATTATCTTGCGGGGGGTATCGAATCCCTGCCGGTAGCGACTGCGACGTTTGATCTTGcc > 1:341668/1‑250 (MQ=255) gATCTACAGCGACAGAGTGCTGACGCATTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGGACCTCCAGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCCATGCTTGTTCAGGCACGCCCGAAGGATGCCGCAGACCATTATCTTGCGGGAGATATAGAAACCCTGGCGTTAGCGACTGCGACGCTCGATCTTGCATg > 1:517851/1‑251 (MQ=255) agTGCTGACGCCTTACTGGAAATGCTTCCACAGCGTAAATACACCCCCGTCCGGGACGCGGGTTGTGG‑CCCGGGCTGGAGGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCgcagt < 1:187150/251‑1 (MQ=255) ccTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACCCGCCGGTGACGGCCTTAGCTCTC‑TCGCCGCCCATGCTTGTTCAGGCACGCCAGAAGGATGCCGCCGACCCTTATCTGGCGGGAGATATCGAATCCCTGCCGTTTGCGGCTGCGGCGGTCGATCTTTCATGGGGCCATCTCGCCGTGCAGTGGTGc > 1:448923/1‑251 (MQ=255) cccACAGCGTAAATACCCCCACGTACGGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACgg < 1:122075/250‑1 (MQ=255) ccACGTACGGGACGCGGGTTGTGG‑CCCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATc < 1:340164/251‑1 (MQ=255) cGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCCGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCCTTGAGCAATCTCGCAGTGCAGGGGTGCGGGAATTTATCCCCCGCACCCCGCGAGCTGGATCggg > 1:160872/1‑251 (MQ=255) gtGGACGGGGGTGGTGG‑ACCTGGTGGGATGATCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCGTTAGATCTC‑GCGCCGCCAATGCTTGTTCAGGCACGCAAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGggtggt < 1:197782/250‑1 (MQ=255) ggccgcgGGTTGTGG‑CCCGGGCTGGATGGGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCCCGCCAGAAGGATCCCGCAGACCATTTTCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGggtggt < 1:449117/246‑1 (MQ=255) ggACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCCCGCGCCGGGGAAGGCCCTAGGTCTC‑TCGCCGCCCATGCTTTTTTCGGCACCCCAGAAGGATGCCGCAGACCATTATCTTGCGGGGGATATCTAAACACTGCCGTTTGCGACCGCGGCGTTCGGACTTGCATGGAGCAAACTCCCAGTGCCGTGGGGGGGGAATTTTTTCCCCGCACTCCCCGAGCTGTATTGGggggtgg > 1:578961/1‑250 (MQ=255) cgcgGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGGCGGCCTTAGATCTC‑TCGCCGGCAATGCTTGTTCCGGCACGCCCGAAGGGTGTCGCCGACCCTTATCTGGGGGGGGAGATAGGATCCCTGCCCGTAGCGGCCTCGACGTTCGATCTTGCCTGGAGCAATCTCGCAGTGCGGGGGGGCGGGAATTTTTCCAAGGCACCCCGCCTGCTGTATCggggggggggcc > 2:522965/1‑242 (MQ=255) gcgGGTTGTGG‑ACCTGGCGGGAGGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCGGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCG‑ccc < 1:230788/251‑1 (MQ=255) gtgggggcccgggtggaagggcccgccACGGGGGGGACCGCCACGCGCGGGGGACGGCCTAAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGCCCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCG‑CCCCaaa < 2:400703/229‑1 (MQ=255) ccTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGGCCTAGATCTC‑TCGCCGCCCATGCTTTTTCAGGCACGCCAGAAGGGGGCCGCCGACCATTTTCTGGCGGGGGAAATCGACTCCCCGCCGGTCGCGGCTGCGACGTTCGGTCTTTCGTGGAGGAATCTCCCCGTGCCGTGGGGCGGGGATTTTTTCACCGCACTCCCCGAGCGGTATCGGGTGGGGCGGCCCCAAGGGCgggg > 2:69565/1‑248 (MQ=255) gtgaaTGAGCCGCCACTGGCGGGAACGTCACGCGCGGGTGCCGGCCTTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCG‑CCCCAAAGGCGTGGTCGCGt < 1:374009/247‑1 (MQ=255) gccgccACGGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCG‑CCCCAAAGGCGTGGTCGCGTTTACCACg < 1:284955/251‑1 (MQ=255) cccACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCG‑CCCCAAAGGCGTGGTCGCGTTTACCACGCTg < 1:168128/250‑1 (MQ=255) cTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCAATGCTTGTTCCGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATCTCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGGGCG‑CCCCAAAGGCGTGGGCGCGTTTACCACGCTGGTGc > 2:283614/1‑251 (MQ=255) ggCGGGCACGACACGCGCAGGTGACGGCTTTAGATCTC‑TCGCCGCCAATGCTTGTGCAGGCACGCCAGAAGGATGCCGAAGACCATTATCGGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCG‑CCCCAAAGGCGTGGTCGCGTTTACCACGCTGGTGCAg < 1:439807/251‑1 (MQ=255) ggCGGGAACGTCACGCGCAGGTGACGGCCTGAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCGCTCCGCGAGCTGTATCGGGTGGTGCG‑CCCCAAAGGCGTGGTCGCGTTTACCACGCTGGTGCAg < 1:107201/251‑1 (MQ=255) ggCGGGAAAGTCACGCGCAGGCGACGGGCTTAGATCTA‑TGGCCGCCAATGCTTGTCAAGGCACGACCGAAGGATGCCGCCGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGCAGTTCGATCTTGCATCGAGCAATCTCGCAGTGCAGTGGCGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGTG‑CCCCCAAGGCGTGGGCGCGttctcccccctggtgccg > 2:403167/1‑235 (MQ=255) gaacgcacggcgcGGGTGCCGGCATTAGACCCC‑CCGCCGCCAAGGCTTGTACAGGCCCGCCAGAAGGATGCCGCAGCCCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCG‑CCCCAAAGGCGTGGTCGCGTTTACCACGCTGGTGCAGGGATc < 2:247528/242‑1 (MQ=255) gAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGCCCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCG‑CCCCAAAGGCGTGGTCGCGTTTACCACGCTGGTGCAGGGATc < 1:522965/251‑1 (MQ=255) aaCGTCACGCGCCGGTGACGGCCTAAGATCTC‑TCGCCGCCAATGATTGTTCAGGCACGCCAGAAGGATGCCGCCGACCATTATCTGGCGGGCGATATCGCATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTCTCCACGGAACTCCGCGAGCTGTCTCGGGTGGTGCG‑CCCCAAAGGCGTGGTCGGGTTTGCCACGCTGGTGCAGGGAt > 2:39440/1‑249 (MQ=255) cGTCACGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCAAGGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCG‑CCCCAAAGGCGTGGTCGCGTTTACCACGCTGGTGCAGGGATCGtt < 1:373405/251‑1 (MQ=255) cgggTGACGGCCTTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAACCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCG‑CCCCAAAGGCGTGGTCGCGTTTACCACGCTGGTGCAGGGATCGTTACCCGAACt < 1:5981/249‑1 (MQ=255) | CTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGG‑ACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTC‑TCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCG‑CCCCAAAGGCGTGGTCGCGTTTACCACGCTGGTGCAGGGATCGTTACCCGAACT > NC_000913/811477‑811967 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |