Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913728,0240TA28.6% 73.9 / 10.2 42N237Y (AAT→TAT) kdpAK(+) transporting P‑type ATPase subunit KdpA
Reads supporting (aligned to +/- strand):  ref base T (28/2);  new base A (0/12);  total (28/14)
Fisher's exact test for biased strand distribution p-value = 8.23e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCACGAAGTTGGTCAGTGCGGTTGGGTTTTCAAACGGATGCGACGAGTTGG‑CATTAAAGAAGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGCTACAGGCCCCATGGGTAACAGCTGTTGCGCTCCTTCAACGGTATTCACAGCCTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAGATCGACCCAGGCATTCCCGAGCGTGCTCATGCTCTGGCGGG  >  NC_000913/727776‑728266
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  
atTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCACGAAGTTGGTCAGTGCGGTTGGGTTTTCAAACGGATGCGACGAGTTGG‑CATTa                                                                                                                                                                                                                                                   >  1:205419/1‑250 (MQ=255)
    aTGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCACGAAGTTGGTCAGTGAGGTTGGGTTTTCAAACGGATGCGACGAGTTGG‑CATTAaaga                                                                                                                                                                                                                                               >  2:127837/1‑250 (MQ=255)
           tgTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCACGAAGTTGGTCAGTGCGGTTGGGTTTTCAAACGGATGCGACGAGTTGG‑CATTAAAGAAGCCAccg                                                                                                                                                                                                                                       >  2:484590/1‑251 (MQ=255)
                     cAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCACGAAGTTGGTCAGTGCGGTTGGGTTTTCAAACGGATGCGACGAGTTGG‑CATTAAAGAAGCCACCGCCGTTAGTAc                                                                                                                                                                                                                             >  2:12528/1‑251 (MQ=255)
                             gcagATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCACGAAGTTGGTCAGTGCGGTTGGGTTTTCAAACGGATGCGACGAGTTGG‑CATTAAAGAAGCCACCGCCGTTAGTACCGAGCATc                                                                                                                                                                                                                     >  2:384526/1‑251 (MQ=255)
                                gATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCACGAAGTTGGTCAGTGCGGTTGGGTTTTCAAACGGATGCGACGAGTTGG‑CATTAAAGAAGCCACCGCCGTTAGTACCGAGCATCTTg                                                                                                                                                                                                                  >  1:558765/1‑251 (MQ=255)
                                   gAGGATTACCCTGAACTTCTGCCCACATCCCCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCACGAAGTTGGTCAGTGCGGTTGGGTTTTCAAACGGATGCGACGAGTTGG‑CATTAAAGAAGCCACCGCCGTTAGTACCGAGAATCTTGATc                                                                                                                                                                                                               >  2:550015/1‑251 (MQ=255)
                                     ggATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCACGAAGTTGGTCAGTGCGGTTGGGTTTTCAAACGGATGCGACGAGTTGG‑CATTAAAGAAGCCACCGCCGTTAGTACCGAGCATCTTGATCGc                                                                                                                                                                                                             >  1:613333/1‑251 (MQ=255)
                                        ttccccTGAACTTCTCCCCACACCACCACGCCTCCGCAGATGACAAAAATCACTGCCACCCCCCACAGCAACAGGCGCCCCGGGCGGCGACCGCCCATCACTCCACCAAAGGCAAAGCACAGCCCCGTTGGAATCAAGAAGATCGCCAGCATTGGCACGGATTTGGTCAGTGCGGTTGGTTTTTCAAAGGGAGGCGACGATTTGT‑AAGTAAAAACGCCACCGCCGTTAGTACCGAGCATCTTGATCGCtt                                                                                                                                                                                                           <  1:361191/247‑1 (MQ=255)
                                          ccccTGAACTTCTGCCCACATCCCCCCGCCGACGCAGATGACAAAAATCACTGCCACCGCCCACAGCAACCGGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCACGAAGTTGGTCAGTGCGGTTGGGTTTTCAAACGGATGCGACGAGTTGG‑CATTAAAGAAGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCt                                                                                                                                                                                                        <  1:379309/250‑1 (MQ=255)
                                                aaCTTCTGCCCACATCACCACGCCTACGCAGATGACCAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCCGCGATAGCCCATCACTTCACCACAGGCAACGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCACGAAGTTGGTCAGTGCGGTTGGGTTTTCAAACGGATGCGACGAGTTGG‑CATTAAAGAAGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTgagagg                                                                                                                                                                                                  >  1:675478/1‑249 (MQ=255)
                                                  cTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCACGAAGTTGGTCAGTGCGGTTGGGTTTTCAAACGGATGCGACGAGTTGG‑CATTAAAGAAGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGCt                                                                                                                                                                                                >  1:328812/1‑251 (MQ=255)
                                                    ccTGCCCACAACCCCACGCCTACGAGGAGGACAAAAACCCCTGCCACCCCCCACAGCAACGGGCCCCCCGGGCGGCGATCGCCCATCCCTCCACAAAAGGCAAAGCACAGCGCGGTGGGGATCAAGAGGATCGCAGGCTCCGGCACGAAGTTGGTCGGTGGGGTTGGGTTTTCAAAGGGAGGCGACGAGTTGG‑CATAAAAGAGGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGCTAc                                                                                                                                                                                              <  1:12369/250‑1 (MQ=255)
                                                     cTGCCCACATCACCACGCCTCCGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCAATCACTTCACCAAAGCCAAAGCACAGCGCCGTTGGGATCAAGAAGCTCGCCAGCATCCGCACGAAGTTGGGCAGTGCGGTTGGGTTTTCAAACGGATGCGACGAGTTGG‑CATTAAAGAAGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGcgacc                                                                                                                                                                                             >  2:246691/1‑247 (MQ=255)
                                                                       cTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCACGAAGTTGGTCAGTGCGGTTGGGTTTTCAAACGGATGCGACGAGTTGG‑CATTAAAGAAGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGCTACAGGCCCCATGGGTAACAGc                                                                                                                                                                           >  1:115256/1‑251 (MQ=255)
                                                                               aTGACAAACATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCACGAAGTTGGTCAGTGCGGTTGGGTTTTCAAACGGATGCGACGAGTTGG‑CATTAAAGAAGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGCTACAGGCCCCATGGGTAACAGCTGTTgcgc                                                                                                                                                                   >  1:491990/1‑251 (MQ=255)
                                                                                    aaaaaTCACTGACACCGCCCACAGCAACAGGCCCCCCTGGCGGCGATCCCCCATCACTTCACAAAAGGAAAAGCACAGCGCCGTTGGGATAAAGAAGAGCGCCAGCATCTGCACGAAGTTGGTCAGTGCGGTTGGTTTTTAAAACGGATGCGACGAGTTGG‑CATAAAAGAAGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGCTACAGGCCCCATGGGTAACAGCTGTTGCGCTCCtt                                                                                                                                                              <  1:384526/251‑1 (MQ=255)
                                                                                       accccctgcccccccccACAGCACAATGCCCCCCGGGCGGCGACCGCCCATCCCTCCACCAAAGGAAAAGCACGGCGCCTTGGGGAAAAAGAAGATCGCCAGCTCCTCCACGAAGTGGGTCGGTGCGGTTGGTTTTACAAACGGAGGCGACGAGTTGG‑CATAAAAGAAGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGCTACAGGCCCCATGGGTAACAGCTGTTGCGCTCCTTCaa                                                                                                                                                           <  2:102515/237‑1 (MQ=255)
                                                                                          cactccctgcGCCCACAGAAACACCCCCCCCGGGCGGCGACCGCCCATCACTCCACCAAAGCAAAAGCACCGCGCCGTGGGGAAAAAGAAGACCGCCGGCATCTGCGCGAGGTTGGTAAGGGCGGTTGGTTTTCCAAAGGGAGGGGACGGGTGGG‑AATAAAAGAGGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGCTACAGGCCCCATGGGTAACAGCTGTTGCGCTCCTTCAACgg                                                                                                                                                        <  2:652591/242‑1 (MQ=255)
                                                                                            cTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCACGAAGTTGGTCAGTGCGGTTGGGTTTTCAAACGGATGCGACGAGTTGG‑CATTAAAGAAGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGCTACAGGCCCCATGGGTAACAGCTGTTGCGCTCCTTCAACGGt                                                                                                                                                       >  1:564571/1‑250 (MQ=255)
                                                                                                                     ccccTGGCGGCGACCGCCCAACACTACCCCAAAGGAAAACCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCACGAAGTGGGTCAGTGCGGTTGGTTTTTCAAACGGATGCGACGAGTTGG‑CATAAAAAAAGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGCTACAGGCCCCATGGGTAACAGCTGTTGCGCTCCTTCAACTGTATTCACAGCCTGATAAGGCAGAAAGt                                                                                                                             <  1:518558/251‑1 (MQ=255)
                                                                                                                                          cACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCACGCCGTTGGTCAGTGCGGTTGGGTTTTCAAACGGATGCGACGAGTTGG‑CATTAACGAAGCCACCGCCGTTCGTACCGAGCATCTTCATCGCTTCCTTCGCAGCTACAGGCCCCCTGGGTACCAGCTTTTTCGCTCCTTCAACGGTATTCACAGCCTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGa                                                                                                         >  1:260804/1‑250 (MQ=255)
                                                                                                                                          cACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCACGACGTTGGTCAGTGCGGTTGGGTTTTCAAACGGATGCGACGAGTTGG‑CATTAAAGAAGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGCTACAGGCCCCATGGGTAACAGCTGTTGCGCTCCTTCAACGGTATTCACAGCCTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGa                                                                                                         >  1:465943/1‑250 (MQ=255)
                                                                                                                                              tCACCAAAGGCAAAGCCAAGCGCCGTGGGGATAAAGAAAATCGCAAGTATCTGCACGAAGTTGGTCAGTGGGGTTGGGTTTTCAAACGGATGGGACTAGTTGG‑CATAAAAGAAGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGCTACAGGCCCCATGGGTAACAGCTGTTGCGCTCCTTCAACGGTATTCACAGCCTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATa                                                                                                      <  1:74089/249‑1 (MQ=255)
                                                                                                                                              tCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCACGAAGTTGGTCAGTGCGGTTGGGTTTTCAAACGGATGCGACGAGTTGG‑CATTAAAGAAGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGCTACAGGCCCCATGGGTAACAGCTGTTGCGCTCCTTCAACGGTATTCACAGCCTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATaa                                                                                                     >  1:574460/1‑250 (MQ=255)
                                                                                                                                               caccagagccaagggccggcgcCGTGGGGATCAAGAAGATCGCCACCATCGACGCGAAGTTGGTCAGTGCGGTGGGGTTTCAAAACGGATGCGGGGA‑TTGGACATAAAAAAAGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGCTACAGGCCCCATGGGTAACAGCTGTTGCGCTCCTTCAACGGTATTCACAGCCTGATAAGGCAGAAAGTGTTGCAGCGCACCTTGTTGAATaaaa                                                                                                   <  1:127837/233‑1 (MQ=255)
                                                                                                                                                   aaaGGCAAAGCACAGCGCCTGGGGGATCAAAAAGACCGCAAGCATCTGCACGAGGTTGGTCAGTGCGGTTGGGTTTCCAACCGGATGCGAGGAGTTGG‑CATAAAAGAACCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGCTACAGGCCCCATGGGTAACAGCTGTTGCGCTCCTTCAACGGTATTCACAGCCTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACa                                                                                               <  1:428841/251‑1 (MQ=255)
                                                                                                                                                              acaGCGCCGTTGGGATCCAGAAGATCGCAAGAATCTGCACGAAGTTGGTCAGTGCGGTTGGGTTTTCAAACGGATGCGACGAGTTGG‑CATTAAAGAAGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGACAAGCTACAGGCCCCATGGGTAACAGCTGTTGCGCTCCTTCAACGGTATTCACAGCCTGATAAGGCAGCAAGTTTTGCAGCGCACCTTGTTGAATCAAAACCAGTGCAATcaac                                                                                    >  2:675383/1‑251 (MQ=255)
                                                                                                                                                                                          cAGCATCTGCACGAAGTTGGTCAGTGCGGTTGGGTTTTCAAACGGATGCGACGAGTTGG‑CATTAAAGAAGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGc                                                                                                                                                                                                 >  2:627192/1‑114 (MQ=255)
                                                                                                                                                                                          cAGCATCTGCACGAAGTGGGTCAGTGGGGTTGGTTTTTCAAAGGGATGCGACGAGTTGG‑CATAAAAGAAGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGCTACAGGCCCCATGGGTAACAGCTGTTGCGCTCCTTCAACGGTATTCACAGCCTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACg                                                        <  1:12528/251‑1 (MQ=255)
                                                                                                                                                                                          cAGCATCTGCACGAAGTGGGTCAGTGCGGTTGGGTTTTCAACCGGAGGCGACGAGTTGG‑CATAAAAGAAGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGc                                                                                                                                                                                                 <  1:627192/114‑1 (MQ=255)
                                                                                                                                                                                                     cGAAGTTGGACAGTGAGGTTGGGTTTTCAAACTGATGCGACGAGTTGG‑CATTACAGAGGCCACCGCCGTTAGAACCGAGCATCTTGATCGCTTCCTGAGAAGCTACAGGCCCCATGGGTAACAGCTGTTGCGCTCCTTCAACGGTATTCACAGCATGATCAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATACAAAACAGTGCAATCAACAACGCCACAGTGACTAGCACCCATAACGTGATGCGTAGc                                             >  2:494742/1‑251 (MQ=255)
                                                                                                                                                                                                               cAGTGCGGTTGGGTTTTCAAACGGATGCGACGAGTTGG‑CATTAAAGAAGACACCGCCGTTCGTAACGAGCATCTTGATCGCTTCCTGAGAAGCTACCGGCCCCATGGGTAACAGCTGTTGCGCTCCTTCAACGGTATTCACAGCCTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAGATCGAccc                                   >  1:347300/1‑251 (MQ=255)
                                                                                                                                                                                                                      gTTGGGTTTTCAAACGGATGCGACGAGTTGG‑CATTAAAGAAGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGCTACAGGCCCCATGGGTAACAGCTGTTGCGCTCCTTCAACGGTATTCACAGCCTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAGATCGACCCAGGCAtt                            >  2:517113/1‑251 (MQ=255)
                                                                                                                                                                                                                        tGGGTTTTCAAACGGATGCGACGAGTTGG‑CATTAAAGAAGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGCTACAGGCCCCATGGGTAACAGCTGTTGCGCTCCTTCAACGGTATTCACAGCCTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAGATCGACCCAGGCATTcc                          >  2:438252/1‑251 (MQ=255)
                                                                                                                                                                                                                             tttCAAAAGGATGCGACGAGTTGG‑CATTAAAGAAGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGCTACAGGCCCCATGGGTAACAGCTGTTGCGCTCCTTCAACGGTATTCACAGCCTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAGATCGACCCAGGCATTCCCGAGc                     >  1:169080/1‑251 (MQ=255)
                                                                                                                                                                                                                              ttCAAACGGATGCGACGAGTTGG‑CATTAAAGAAGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGCTACAGGCCCCATGGGTAACAGCTGTTGCGCTCCTTCAACGGTATTCACAGCCTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGc                                                  >  1:248614/1‑221 (MQ=255)
                                                                                                                                                                                                                                  aacggatgcgtgttgTTGC‑CATAAAAGACGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGCTACAGGCCCCATGGGTAACAGCTGTTGCGCTCCTTCAACGGTATTCACAGCCTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAGATCGACCCAGGCATTCCCGAGCGTGCt                <  2:472775/237‑1 (MQ=255)
                                                                                                                                                                                                                                     ggATGCGCCGAGTTGG‑CATTAAAGAAGACCCCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGCTACAGGCCCCATGGGGAACAGCTGTTGCGCTCCTTCAACGGTATTCACAGCCTGATAAGGCAGAAAGTTTTGCAGCGAACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTCGCACACATAACGTGATGCGTAGCAGATCGACCCAGACATTCCAGAGCGTGCTCAt             >  2:400665/1‑251 (MQ=255)
                                                                                                                                                                                                                                              gaGTTGG‑CATTAAAGAAGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGCTACAGGCCCCATGGGTAACAGCTGTTGCGCTCCTTCAACGGTATTCACAGCCTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAGATCGACCCAGGCATTCCCGAGCGTGCTCATGCTCTGGCg    >  1:133062/1‑251 (MQ=255)
                                                                                                                                                                                                                                                gTTGG‑CATTACAGAAGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGCTACAGGCCCCATGGGTAACAGCTGTTGCGCTCCTTCAACGGTATTCACAGCCTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAGACCGACCCAGGCATTCCCGAGCGTCCTCATGCCCTTGCggg  >  2:252782/1‑251 (MQ=255)
                                                                                                                                                                                                                                                gTTGG‑CATAAAAGAAGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGCTACAGGCCCCATGGGTAACAGCTGTTGCGCTCCTTCAACGGTATTCACAGCCTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAGATCGACCCAGGCATTCCCGAGCGTGCTCATGCTCTGGCggg  <  1:227115/251‑1 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  
ATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCACGAAGTTGGTCAGTGCGGTTGGGTTTTCAAACGGATGCGACGAGTTGG‑CATTAAAGAAGCCACCGCCGTTAGTACCGAGCATCTTGATCGCTTCCTGAGAAGCTACAGGCCCCATGGGTAACAGCTGTTGCGCTCCTTCAACGGTATTCACAGCCTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAGATCGACCCAGGCATTCCCGAGCGTGCTCATGCTCTGGCGGG  >  NC_000913/727776‑728266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑