| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 288,382 | 0 | A | C | 31.8% | 81.7 / 10.6 | 65 | T532P (ACC→CCC) | yagH | putative xylosidase/arabinosidase |
| Reads supporting (aligned to +/- strand): ref base A (24/19); new base C (1/19); total (25/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.99e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGAGCAGCCCATTCCGGTGCCGGAACATGCGGAGAGCGTCTGGCTGCGGGTGGACGTGGATACGCTGGTCTACCGCTACAGCTACTCGTTTGATGGCGAGACGTGGCACACCGTGCCGGTGACGTATGAGGCGTGGAAGCTGTCGGACGACTACATCGGCGGGCGCGGCTTCTTCACCGGCGCG‑TTTGTGGGCCT‑GCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCAC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCAC‑ACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCG > NC_000913/288135‑288632 | cGAGCAGCCCATTCCGGTGCCGGAACATGCGGAGAGCGTCTGGCTGCGGGTGGACGTGGATACGCTGGTCTACCGCTACAGCTACTCGTTTGATGGCGAGACGTGGCACACCGTGCCGGTGACGTATGAGGCGTGGAAGCTGTCGGACGACTACATCGGCGGGCGCGGCTTCTTCACCGGCGCG‑TTTGTGGGCCT‑GCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCAC‑Ct < 1:375823/251‑1 (MQ=255)cGAGCAGCCCATTCCGGTGCCGGAACATGCGGAGAGCGTCTGGCTGCGGGTGGACGTGGATACGCTGGTCTACCGCTACAGCTACTCGTTTGATGGCGAGACGTGGCACACCGTGCCGGTGACGTATGAGGCGTGGAAGCTGTCGGACGACTACATCGGCGGGCGCGGCTTCTTCACCGGCGCG‑TTTGTGGGCCT‑GCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCAC‑Ct < 1:272100/251‑1 (MQ=255) ggtcgcGGACCATGCGGAGGGCGTCGTGCGGCGGGTGGACGGGGTTACGTGGGGCTCCCGCTACAGCTACTCGTTTGAGGGGGAGACGGGGCACACCGTGCCGGTGCCGTATGAGGCGTGGAAGCGGGCGGACGACTACACCGGCGGGCGCGGCTTCTTCCCCGGCGCG‑TTTGTGGGCCT‑GCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGATTTCGACTACTTCAC‑CTACGAGCCGGTCTAAc < 2:27898/246‑1 (MQ=255) ggcggcGGAACGTGGGGAGTGCGTCTGGCGGCGGGGGGACGGGGACACGCTGGTCTACCGCTACAGCTACTCGTTTGAGGGGGAGACGTGCCCCACCGTGCCGGTGCCGTTGGAGGCGTGGAAGCTGTCGGACGACTCCACCGGCGGGCCCGGTTTCTTCACCGGCGCG‑TTTGTGGGCCT‑GCACTGCGAGGCCAGCACCGGCGACGGCTGCTCCGCGGCCTTCGACTACTTCCC‑CTCCGAGCCGGTCTAAc < 2:507615/246‑1 (MQ=255) ggTGCCGGATCATGCGGACGGCGTCTGGCGGCGGGTGGACGTGGATCCGCTGGTCTCCCGCTACAGCTACTCGTTGGAGGGCGAGACGTGGCACACCGTGCCGGTGACGTAGGAGGCGGGGAAGCTTTCGGACGACTACACCGGCGGGCGCGGCTTCTTCCCCGGCGCG‑TTTGTGGGCCT‑GCACTGCGAGGACAGCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCAC‑CTCCGAGCCGGTCTAAc < 2:240362/251‑1 (MQ=255) aCATGCGGAGAGCGTCTGGCTGCGGGTGGACGTGGATACGCTGGTCTACCGCTACAGCTACTCGTTTGATGGCGAGACGTGGCACACCGTGCCGGTGACGTATGAGGCGTGGAAGCTGTCGGACGACTACATCGGCGGGGGCGGCCTCTTTACCGGCGCGGTTTTTGGGC‑T‑GCACCGCGGGGGCACCCGCGGGGCCGGGTGCTACCCGGGCTTCGCATCCTTCAC‑Ctcccagacgggcttaaagccccagc > 1:458319/1‑227 (MQ=255) caggcggggggCGTCGGGCGGCGGGGGGACGTGGATACGCTGTTCACCCGCTACAGCCACCCGTTGGATGGCGAGAGGTGGCCCACCGTGCCGGTGACGTATGAGGCGTGGAAGCTGTCGGACGACTACATCGGGGGGCGCGGCTTCTTCACCGGCGCG‑TTTGGGGGCCT‑GCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCAC‑CTGCGAGCCGGTCTAACGGCTCAGGcc < 2:248555/241‑1 (MQ=255) ggggggACGTGGATACGCGGTTCTACCGCTACAGCTACTCGTTTGATGGCGGGACGTGGCACCCCGTGCCGGTGACGTATGAGGCGTGGAAGCTGTCGGACGACTACATCGGCGGGCGCGGCTTCTTCCCCGGCGCG‑TTTTTGGGCCT‑GCACGGCGAGGACATCAGCGGCGTCGGTTGCTACGCGGACTTCGACTACTTCTC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑TGGTAGCCCAGCGCGCGCgaga < 1:429233/247‑1 (MQ=255) gACGGGGATCCGCGGGTCTCCCGCTACAGCTCCTCGTTTGAGGGCGAGACGGGCCACCCCGGGCCGGTGCCGTATGAGGCGTGGAAGCTGTCGGACGACTACATCGGCGGGCGCGGCTTCTTCACCGGCGCG‑TTTGTGGGCCT‑GCACTGCGGGGACATCAGCGGCGACGGCTGCTACGCGGATTTCGACTACTTCAC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGcgcg < 2:340718/251‑1 (MQ=255) gCTGGTCTCCCGTTACAGCTACTCGTTGGATGGCGAGACGTGGCCCCCCGTGCCGGTGACGTATGAGGCGTGGAAGCTGTCGGACGACTACATCGGGGGGCGCGGCTTCTTCCCCGGCGCG‑TTTGTGGGCCT‑GCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCCC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCctg < 1:61654/251‑1 (MQ=255) gCTGGTCTACCGCTACAGCTACTCGTTTGATGGCGAGACGTGGCACCCCGTGCCGGTGACGTATGAGGCGTGGAAGCTGTCGGACGACTACATCGGCGGGCGCGGCTTCTTCACCGGCGCG‑TTTGTGGGCCT‑GCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCCC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCctg < 1:227542/251‑1 (MQ=255) gttCTACCGCTACAGCTACCCGTTTGATGGCGAGACTTGGCACACCGTGCCGGTGTCGTAGGAGGCGTGGAAGCTGGCGGACAACTACATCGGCGGGCCCGGCTTCTTCACCGGCGCG‑TTGGTGTGCCT‑GCACTGCGAGTGCATCAGCGGCGGGGGCTGCTACGCGCACTGCGCCTTCTTCCC‑CGACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCctgc < 1:407723/247‑1 (MQ=255) tACAGCTACTCGTTTGATGGCGAGACGTGGCACACCGTGCCGGTGACGTATGAGGCGTGGAAGCTGTCGGACGACTACATCGGCGGGGGCGGGCTCTTCTCCCGCGCG‑GTTTTGGGGCT‑GCCCTGCGAGGGAATCAACGGCGGCGGCCGCTACGCCGGCGTCGGCCACCTCAC‑CTACCAACCGAGCCAACGGCCCAGGCCCGGGT‑GCCCCCCGCGCGCGAGAGGGCGGGCCCGGccccctgacgaaccccc > 1:561154/1‑236 (MQ=255) tCGTTTGATGGCGGGACGGGGCCCCCCGGGCGGGTGACGTATGAGGCGGGGAAGCTGTCGGACGACTACATCGGCGGGCGCGGCTTCTTCACCGGCGCG‑TTTGGGGGCCT‑GCACAGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGAATACTTCCC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAgct < 1:307487/237‑1 (MQ=255) tgttggATGGCGAGACGGGGCACACGGCGCCGGTGACGTATGAGGCGGGGAAGTGGTCGGACTCCTACACGGGCGGTCGCGGCTTCTTCCCCGGCGGG‑TTTGTGGGCCT‑GCACTGCGAGTACTCCAGGGGCGATGGCTGCTACGCGGACTTCGACTCCTTCCC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCTGAAAtt < 1:331082/244‑1 (MQ=255) gttggatggggaggggaggCACGCCGTGCGGGTGCCGTATGGGGCGGGGAAGCTCTGGGCCGGCAAGTTGGGCGGGCGGGGCTTGTGCTCGGGCGCG‑TTTGGGGGCCT‑GCACTGCGGGCAGTTCGGCGGGGACGGCTGTGACGGGGACTTCTACTGCTTCAC‑TTGCGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGGGCGAGAGCGCGAGCCCGGCCTGCGGAAGCGGCTCGCGGAAATTAGc < 1:575286/234‑1 (MQ=255) ccGTGGCACCCCGTGCCGGTGACGTATGAGGCGTGGAAGCTGTCGGACGACTACATCGGCGGGCGCGGCTTCTTCACCGGCGCG‑TTGGTGGGCCT‑GCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTGCAC‑CTCCGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGcgtcg < 1:303792/250‑1 (MQ=255) gggCACACCGTGCCGGTGACGTATGAGGCGTGGAAGCTGTCGGACGACTACATCGGCGGGCGCGGCTTCTTCACCGGCGCG‑TTTGTGGGCCT‑GCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCAC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTcc < 1:447461/250‑1 (MQ=255) ggCCGGTGACGTATGAGGCGGGGAAGCTGTCGGACGACTGCATCGGCGGGCGGGGCTTCTTCACCGGCGCG‑TTTGTGGGCGT‑GCGCGGGGAGGACATCAGCGGCGACGGCTGCTACGCGGATTTCGACTACTGCAC‑CTCCGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCGTCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAgg < 1:597131/250‑1 (MQ=255) cGTAGGAGGCGTGGAAGCTGGCGGACGACTACATCGGCGGGCGCGGCTTCTTCCCCGGCGCG‑TGTGTGGGCCT‑GCACTGCGAGGACATCAGCGGCGCCGGCTGCTACGCGGACTTCGACTACTTCCC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCg < 1:385260/251‑1 (MQ=255) ggCTGTCGGGCGCCTCCATCGGCGGGCGCGGCTTCTTCCCCGGCGGG‑TGTGTGGGCCT‑GCACTGGGAGGACACCAGCGGCGAGGGCGGCTACGCGGACTTGGTCTACTTCAC‑CAACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGggcgg < 1:129554/250‑1 (MQ=255) cGGACGACTACATCGGCGGGCGCGGCTTCTTCACCGGCGCG‑TTTGTGGGCCT‑GCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGTCTTCGACTACTCCAC‑CTCCGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTcc < 1:339152/211‑1 (MQ=255) ggACGCCTACAGCGGCGGGCGCGGCTTTTTCACCGGCGCG‑TTTGTGGGCCT‑GCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTCCAC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGAc < 1:512501/251‑1 (MQ=255) acgacTACATCGGCGGGCGCGGCTTCTACACCGGCGCG‑TTGTTGGGCCT‑GCACTGCGAGGACATCAGCGGCGACGGCTGCTCCGCGGACTTCGACTACTTCCC‑CGACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGAcgc < 1:26422/251‑1 (MQ=255) gacTACACCGGCGGGCGCGGTTTCTCCCCCGGCGCG‑TTTGTGGGCCT‑GCCCTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTCCGACTACTCCAC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCgg < 1:542743/251‑1 (MQ=255) gttaccgcggcggGCCGGGCTTCTTCCCGGGCGCG‑TTTGTGGGCCT‑GCTCTGCGGGGGCATCAGCGGGTGCGGCTGGTGCGCGGACTGTGACTCCTTCCC‑CTACGAGCCGGTCTAACGGCGCAGTCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGTCCTTCTGAAGCTGCTCGCGGAAAGTAGCCGGCTCCGCGTCGTCCACGCGTGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCggg < 1:623203/244‑1 (MQ=255) ccATCGGCGGGCGCGGCTTCTTCCCCGGCGCG‑TTTGGGGGCCT‑GCACTGCGGGGACATCAGCGGCGGCGTCGGCTACGCGGACTTCGACTACTTCGC‑CTGCGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGa < 1:462814/248‑1 (MQ=255) aTCGGCGGGCGCGGTTTCTTCACCGGCGCG‑TTTGGGGGCCT‑GCACTGCGAGGACATCAGCGGCGCCGGCTGCTACGCGGATTCCGACTCCTCCCC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCg < 1:1495/251‑1 (MQ=255) cggcggGCGCGGCTTCTTCACCGGCGCG‑TTGGGGGGCCT‑GCACGGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCCC‑CTACGAGCCGGTCTAACAGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTg < 1:529793/251‑1 (MQ=255) cggcggGCGCGGCTTCTCCACCGGCGCG‑TTTGTGGGCCT‑GCATTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGATTTCGACTACTTCCC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTg < 1:91471/251‑1 (MQ=255) gggggcgCGGCTTCTTCCCCGCCTCG‑TTTGTGGGCCTCGC‑CTGCAAGCCCTCCAGCGGCGAGGGCTGCTCCGCGGACTTCAACTCCTTCCC‑CTCCGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTTAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGTATGTCATCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGt < 1:643686/249‑1 (MQ=255) gggcgCGCCTTCGTCCCCGGCGGG‑TTTGTGGTACT‑GCTCCGCGAGTATAGCGGTGGCGACGGCTCCAACGCGGACTTCGACGACCTCACGCT‑CGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGTGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTc < 1:356568/251‑1 (MQ=255) gcgGCTTCTTCACCGGCGCG‑TTTGTGGGCCT‑GCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCAC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCcac > 1:545416/1‑250 (MQ=255) gcgGCTTCTTCACCGGCGCG‑TTTGTGGGCCT‑GCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCAC‑CTACGAGCCGGTCTAACGGCGCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGACCTTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCCGCGCCGACAGGCTCCGGGCGGGGGTGACGCGGGACCCGTGGTTCcaac > 2:25554/1‑249 (MQ=255) ttcACCGGCGCT‑TTTGTGGGCCT‑GCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCCC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCTTGGTTCCAC‑ACCGGCAc < 1:543266/250‑1 (MQ=255) gcgcG‑TTTGTGGGCCT‑GCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCAC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGCGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACCGGCCCAGTGCGGCGATGACGCGGGTCTCGGGGTTCCCC‑ACCGGGCCCGCCACGc > 2:4058/1‑251 (MQ=255) gcg‑TTTGGGGGCCT‑CCACTGCGAGGACATCGGGGGCGCCGGCTGCTACGCGGACTTCGACTCCTCCCC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGGGATGACGCGGGACTCGTGGTTCCAC‑ACCGGCACCGCCACGCAg < 1:68939/251‑1 (MQ=255) g‑TTTGTGGGCCT‑GCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCAC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCCC‑ACCGGCCCCGCCACGCcgcg > 2:130260/1‑251 (MQ=255) gtgGGCCT‑GCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCAC‑CTACGAGCAGGTCTAACGGCTCAGGCC‑GGGTAGCCCCGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCAC‑ACCGGCACCGCCACGCAGCGCACg > 1:645609/1‑251 (MQ=255) ccT‑GCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCAC‑CTACGCGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTACCC‑ACCGGCCCCGCCCCGCAGCGCACGCCCTg > 2:245405/1‑251 (MQ=255) t‑GCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCCC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGCAGCTGCTCGCGGGAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGGGGTTCCCC‑ACCGGCACCGCCACGCCGCGCACGCCCTGcc > 2:409574/1‑250 (MQ=255) gcccggCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTCCTTCCC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCAC‑ACCGGCACCGCCACGCAGCGCACGCCCTGCTc < 1:145761/246‑1 (MQ=255) gCACGGCGAGGACATCAGCGGCGCCGGCTGCTACGCGGACTTCGACTCCTTCCC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCAC‑ACCGGCTCCGCCACGCAGCGCACGCCCTGCTc < 1:162489/251‑1 (MQ=255) cccTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCAC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAg < 1:277673/170‑1 (MQ=255) cACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCAC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAg > 2:277673/1‑172 (MQ=255) ggAGGACACCAGGGGCGCCGGCTGCTACGCGGACTTCGATTACTTCCC‑CTCCGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCAC‑ACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTtctc < 1:333319/250‑1 (MQ=255) aGGACACCGGCGGCGACGGCGGCTCCGCGGACTTCGACTCCTCCAC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCAC‑ACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCtt < 1:95916/251‑1 (MQ=255) ggACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCAC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCAC‑ACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTc > 1:415003/1‑251 (MQ=255) accggcggcgACGCCTGCTCCGCGGCCGTCGACTACTCCAC‑CGACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTc < 2:433109/154‑1 (MQ=255) aTCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCAC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTc > 1:433109/1‑158 (MQ=255) aGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCAC‑CTACGAGACGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGCGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGCAATTCGCCCGCTACGCGTCGTCCACGCGGGAGGTCAGCGTCGCCAGGCTCCGGGCGGCGATGACGCGGGACTCGTGGGTCCACCACGGGC‑CCGCCACGCCGCGCCAGCCCTGCTCGTTCTCTTGGCTGTcc > 2:352883/1‑251 (MQ=255) ggcgACGGCTGCTACGCGGACTTCGACTACTTCAC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCAC‑ACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAgg > 1:157064/1‑251 (MQ=255) gcgACGGCTGCTACGCGGACTTCGACTACTTCAC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCAC‑ACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAggg > 1:251738/1‑251 (MQ=255) gACGGCTGCTACGCGGACTTCGACTACTTCAC‑CTACGAGCCGGTCTAAAGGCTCAGGCC‑GGGTAGCCCAGCGCGCGAGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCAC‑ACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCg > 1:41984/1‑251 (MQ=255) ggCTGCTACGCGGACATCGACTAATTCAC‑CTACGAGCCGGTCTAACGCCTCAAGCC‑GGGGAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGACATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAACGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCCC‑ACCGGCACCGCCACGCCGCGCACGCCCTGCCCGCGCTCTTCGCTGTCCCGGGCgg > 2:437449/1‑248 (MQ=255) gACTTCGAATACTTCAC‑CTCCGAGACGGTCTCACGGCTCAGGCA‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTACTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCAC‑AACGGCACCGCCACGCCGCGCAAGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCgg > 2:661170/1‑251 (MQ=255) aCTTCGACTACTTCAC‑CTAAGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGGTCCac‑ac > 2:94476/1‑183 (MQ=255) aCTTCGACTACTCCAC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCac‑ac < 1:94476/183‑1 (MQ=255) tCGACTACTTCCC‑CTCCGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCAC‑ACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTc < 1:206657/250‑1 (MQ=255) cGACTACTTCAC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCAC‑ACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTg > 1:148744/1‑251 (MQ=255) gACTACTTCAC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGCCTCGTGGTTCCAC‑ACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTgc > 2:154535/1‑251 (MQ=255) gACTACTTCAC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCCC‑ACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTgg > 2:639255/1‑250 (MQ=255) actTCAC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCACAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCAC‑ACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGccc > 1:55488/1‑250 (MQ=255) actTCAC‑CTACGAGCCGGTCTAACGGATCAGGCA‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGACCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGGGGTTCCAC‑ACCGGCTCCGCCACGCAGCGCACGCCCTGCGCGTTCTCTTCGCGGTCCAGGGCGTAGCCTTGCTCGCGGGTCCGCGcc > 2:269601/1‑250 (MQ=255) tCAC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGGCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCAC‑ACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCTggg > 2:215580/1‑251 (MQ=255) aC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCAC‑ACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGgcg > 1:118233/1‑251 (MQ=255) | CGAGCAGCCCATTCCGGTGCCGGAACATGCGGAGAGCGTCTGGCTGCGGGTGGACGTGGATACGCTGGTCTACCGCTACAGCTACTCGTTTGATGGCGAGACGTGGCACACCGTGCCGGTGACGTATGAGGCGTGGAAGCTGTCGGACGACTACATCGGCGGGCGCGGCTTCTTCACCGGCGCG‑TTTGTGGGCCT‑GCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCAC‑CTACGAGCCGGTCTAACGGCTCAGGCC‑GGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCAC‑ACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCG > NC_000913/288135‑288632 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |