Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,600,215	+C	intergenic (‑26/+23)	yjjA ← / ← dnaC	DUF2501 domain‑containing protein YjjA/DNA replication protein DnaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,600,214	1	.	C	100.0%	255.1 / NA	71	intergenic (‑25/+24)	yjjA/dnaC	DUF2501 domain‑containing protein YjjA/DNA replication protein DnaC
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (37/34);  total (37/34)

TGTTCATGTTATCTGCGCTTAAGGCCTGGTTTCCGCTGCTAAGCAAGTTAGTTAATGACGCGAGCGACCAACCGCCTTCCTGTGTCGTGCTGTTTTGGTTGCCAAGTTCGCTGGCGGCGCTGGAGAGCGCATCTTTCCAGGACGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCG‑CAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGTATTTC  >  NC_000913/4599970‑4600445                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                        cgtgcgtgtggtcaccggtatcagtttcggttttgtcttctacgtactggaccagatcttcggcccgctgacgttggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTgtcg‑ccagt                                                                                                                                                                                                                                    >  1:105066‑M2/239‑246 (MQ=255)        gtggtcaccggtatcagtttcggttttgtcttctacgtactggaccagatcttcggcccgctgacgttggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaacgaatgaacgaataaagggagcgaaagctccattttttattgttagcaaagtgtgcttcgttcattcctgaTGGGTCGCCAGTATACCGcc                                                                                                                                                                                                                             >  2:265018‑M2/232‑251 (MQ=255)          ggtcaccggtatcagtttcggttttgtcttctacgtactggaccagatcttcggcccgctgacgttggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggatcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCa                                                                                                                                                                                                                            <  1:76770‑M2/21‑1 (MQ=255)          ggtcaccggtatcagtttcggttttgtcttctacgtactggaccagatcttcggcccgctgacgttggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGcc                                                                                                                                                                                                                             >  1:618211‑M2/230‑249 (MQ=255)                 ggtatcagtttaggttttgtcttctacgtactggaccagctcttcggcccgctgacgttggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttattaatcagcctgtggctgttaatgagacaatcgtaaccgaatgaacgaatcaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTTTCGCCAGGATACCGACAGAAACggg                                                                                                                                                                                                                    >  2:677197‑M2/223‑251 (MQ=255)                     tcagtttcggttttgtcttctacgtactggaccagatcttcggcccgctgacgttggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGAtt                                                                                                                                                                                                                >  1:572413‑M2/219‑251 (MQ=255)                        gtttcggttttgtcttctacgtactggaccagatcttcggcccgctgacgttggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAAt                                                                                                                                                                                                             >  2:282681‑M2/216‑251 (MQ=255)                          ttcggttttgtcttctacgtactggaccagatcttcggcccgctgacgttggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATa                                                                                                                                                                                                            <  1:308556‑M2/37‑1 (MQ=255)                                tttgtcttctccctcctggaccaaatcttcggccccgtgccgttggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaagaaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCttt                                                                                                                                                                                                      <  2:221730‑M2/43‑1 (MQ=255)                                tttgtcttctacgtactggaccagatcttcggcccgctgacgttggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCttt                                                                                                                                                                                                      >  1:532199‑M2/208‑250 (MQ=255)                                tttgtattctacgtactggcccagatcttcggcccgctgacgttggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCttt                                                                                                                                                                                                      >  1:506318‑M2/208‑250 (MQ=255)                                   gtcttctacatactggcccagatcttcggcccgctgccgttggtttatggcataccgccgatcatcggcgcactgttgcccagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggcgcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCt                                                                                                                                                                                                  >  2:323405‑M2/205‑251 (MQ=255)                                      ttctacgtactggaccagatcttcggcccgctgacgttggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaataagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGt                                                                                                                                                                                                <  1:468304‑M2/49‑1 (MQ=255)                                          acgtactggaccagatcttcggcccgctgacgttggtttatggcaccccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTAccc                                                                                                                                                                                           <  1:453959‑M2/54‑1 (MQ=255)                                                           ttcggcccgctgacgttggtttatggcatcccgccgatcatcggagcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATcc                                                                                                                                                                          >  1:67462‑M2/181‑251 (MQ=255)                                                            tcggcccgctgacgttggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcatcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTAt                                                                                                                                                                            <  1:325285‑M2/69‑1 (MQ=255)                                                            tcggcccgctgacgtgggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaatctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATc                                                                                                                                                                           <  1:610502‑M2/70‑1 (MQ=255)                                                               gcccgctgacgttggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGt                                                                                                                                                                      >  1:355368‑M2/177‑251 (MQ=255)                                                                     tgacgttggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGa                                                                                                                                                                   >  1:195032‑M2/171‑248 (MQ=255)                                                                                tatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACaa                                                                                                                                                      >  1:7185‑M2/160‑250 (MQ=255)                                                                                  tggcttcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcgtcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGAGCACCCACAAAc                                                                                                                                                    <  2:654910‑M2/93‑1 (MQ=255)                                                                                    gcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttactgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGACGATCACCCACAAACTGt                                                                                                                                                 >  2:260766‑M2/156‑251 (MQ=255)                                                                                    gcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGt                                                                                                                                                 >  2:16646‑M2/156‑251 (MQ=255)                                                                                     catcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGtt                                                                                                                                                <  1:135789‑M2/97‑1 (MQ=255)                                                                                        cccgccgatcatcggcgccctgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTAcc                                                                                                                                             <  2:355368‑M2/100‑1 (MQ=255)                                                                                         ccgccgatcatcggcgctctgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTGCCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTAccc                                                                                                                                            <  2:234397‑M2/101‑1 (MQ=255)                                                                                                    cggcgcacggttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATAc                                                                                                                                  <  2:198132‑M2/111‑1 (MQ=255)                                                                                                     ggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATc                                                                                                                                                              <  1:78464‑M2/83‑1 (MQ=255)                                                                                                     ggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATc                                                                                                                                                              >  2:78464‑M2/139‑221 (MQ=255)                                                                                                       cgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgcatgaacgaataaagggagcgaaagctccctttattattgatagcaaagtgtgcttcgttcattcctgaTGTGTCGCCGGTATACCGCCAGAAACGGGGATTAATAATCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACCAACTGTTACCCAGGCGCAtacgttc                                                                                                                              >  2:28839‑M2/137‑248 (MQ=255)                                                                                                       cgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGGATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTc                                                                                                                              <  1:420530‑M2/115‑1 (MQ=255)                                                                                                        gcactgttgccaagcgccagcttctgcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctcccgttattattgttagcaaagtgtgcttcgttcagtccggaTGTGTCGCCAGGATACCGCCAGAAACGGGGATTAATACTCTTTTCCTGTTACCCGGCTACGGTAGCTATGCCAGTTGAATATCACCCACAAACTGTTACCCAGGCGCATACGGTcc                                                                                                                             <  1:620311‑M2/116‑1 (MQ=255)                                                                                                         cactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTcc                                                                                                                             >  1:625467‑M2/135‑250 (MQ=255)                                                                                                               tgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGTCTCATACGGTCCATCAcg                                                                                                                       <  2:618544‑M2/122‑1 (MQ=255)                                                                                                                   aagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTc                                                                                                                  <  1:260766‑M2/127‑1 (MQ=255)                                                                                                                          agcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCAAGc                                                                                                           >  2:491049‑M2/118‑251 (MQ=255)                                                                                                                            cttcttcttaatcagcctgtggctgctaatgagaaactcgtaaccgaatgaacgactaaagggagcgaaagctccctttattattgttagcaaagtgagcttcgttcattcctgcTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGATATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGcac                                                                                                         >  2:60684‑M2/116‑250 (MQ=255)                                                                                                                                 tcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACt                                                                                                       <  2:532199‑M2/138‑1 (MQ=255)                                                                                                                                    taatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGt                                                                                                   <  2:431190‑M2/142‑1 (MQ=255)                                                                                                                                         agcctgtgactgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctcccgttattattgttagcaaagtgttcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCGTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTGGTAGATCACCCACAAACTGTTGCCCAGGCGCATACGTTCCATCACGCGTTCGCTCAGCAACGTGGTCATctct                                                                                            <  2:350573‑M2/149‑1 (MQ=255)                                                                                                                                              gtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCat                                                                                       <  1:13180‑M2/154‑1 (MQ=255)                                                                                                                                                ggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCatat                                                                                     >  1:483312‑M2/96‑251 (MQ=255)                                                                                                                                                 gctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGGCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATAtt                                                                                    <  1:677197‑M2/157‑1 (MQ=255)                                                                                                                                                         tgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTgg                                                                            >  1:277731‑M2/87‑251 (MQ=255)                                                                                                                                                          gagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGt                                                                           >  1:266077‑M2/86‑251 (MQ=255)                                                                                                                                                                        cgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcatgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTACTACTCATTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACCAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATACCGGTTg                                                             >  1:52167‑M2/72‑251 (MQ=255)                                                                                                                                                                         gaatgaacgaataaagggagcgaaagctccctttattattgttagcaacgtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTgg                                                            >  2:596263‑M2/71‑251 (MQ=255)                                                                                                                                                                           atgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGc                                                          <  2:195032‑M2/183‑1 (MQ=255)                                                                                                                                                                                cgaataaagggagcgaaagctccctttcttattgttagcacagtgtgcttcgatcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTCCTGTTGGTCAGCATCCCGGTTGGGCGTTTg                                                     >  1:584928‑M2/64‑251 (MQ=255)                                                                                                                                                                                    taaagggagcgaacgctccctttattattgttagcaacgtgtgcttcgttcattactgaTGTGTCGCCAGTATACCGCCAGACACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACACACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGga                                                   >  2:630016‑M2/60‑249 (MQ=255)                                                                                                                                                                                       agggagcgaaagctctccttattattgttagaaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCGAGAAGCGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTATCTATCCCAGTGGAAGATCACCCCCAAACTGTTACCCATGCGCAGACGGTCCATCACGCGTTCGCACCGCAACTAGTTCATCTCTTCCATAGTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGaagaag                                              <  2:360932‑M2/195‑1 (MQ=255)                                                                                                                                                                                           agcgcaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAgcg                                          >  2:531354‑M2/53‑251 (MQ=255)                                                                                                                                                                                           agcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTTCCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCTACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTGCTCCCAGCAGCTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAgcg                                          <  1:672717‑M2/199‑1 (MQ=255)                                                                                                                                                                                               aaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAgcggcg                                       >  1:23958‑M2/49‑250 (MQ=255)                                                                                                                                                                                                     ccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATcgac                                 <  1:485036‑M2/208‑1 (MQ=255)                                                                                                                                                                                                       ctttattattgttagcaaagtgtgcttagttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGAt                              >  2:655586‑M2/41‑251 (MQ=255)                                                                                                                                                                                                        tttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATc                             >  1:281404‑M2/40‑251 (MQ=255)                                                                                                                                                                                                            ttattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAgcg                                          >  1:196857‑M2/36‑234 (MQ=255)                                                                                                                                                                                                            ttattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAgcg                                          <  2:196857‑M2/199‑1 (MQ=255)                                                                                                                                                                                                              attgttagcaaagtgtgcttcgttcattccagaTGTGTCGCCCGTATACCGCCAGAAACGGGCACTCATAATCTTTACCTGTTACCCGGCACCGATAGCCATCCCAGTTGAAGATCACACACACACTGTTACCCAGGCGCATACGGTCAATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATCTTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGCAGAAGAGCGGCGATCGAAGATCTGGTtg                       >  1:66110‑M2/34‑251 (MQ=255)                                                                                                                                                                                                                 gttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTtgat                     <  1:655586‑M2/220‑1 (MQ=255)                                                                                                                                                                                                                   tagcaaagcgtgcttcgttcattcctgaTGTGTCGCCAGTCTACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCACCTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTgatgat                  >  2:407430‑M2/29‑251 (MQ=255)                                                                                                                                                                                                                    agcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATc                 <  2:514607‑M2/224‑1 (MQ=255)                                                                                                                                                                                                                         agtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACttt            >  2:376175‑M2/23‑251 (MQ=255)                                                                                                                                                                                                                         agtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACttt            <  1:122101‑M2/229‑1 (MQ=255)                                                                                                                                                                                                                            gtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACTTTTTc         >  1:165286‑M2/20‑251 (MQ=255)                                                                                                                                                                                                                            gggcttcgttcattccttaTGTGTCGCCAGTATTCCGCCAGAAACGGGGATTAATACTCTTTGCCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACttttt          <  2:506318‑M2/231‑1 (MQ=255)                                                                                                                                                                                                                                  cgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGtt                                                                              >  2:331179‑M2/14‑176 (MQ=255)                                                                                                                                                                                                                                  cgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGtt                                                                              <  1:331179‑M2/163‑1 (MQ=255)                                                                                                                                                                                                                                  cgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGTAtt    <  2:105066‑M2/237‑1 (MQ=255)                                                                                                                                                                                                                                   gttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCCGTTGAAGATCACCCACACACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGTATTTc  >  2:644172‑M2/13‑251 (MQ=255)                                                                                                                                                                                                                                   gttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGTATTTc  <  2:281404‑M2/239‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                        TGTTCATGTTATCTGCGCTTAAGGCCTGGTTTCCGCTGCTAAGCAAGTTAGTTAATGACGCGAGCGACCAACCGCCTTCCTGTGTCGTGCTGTTTTGGTTGCCAAGTTCGCTGGCGGCGCTGGAGAGCGCATCTTTCCAGGACGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCG‑CAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGTATTTC  >  NC_000913/4599970‑4600445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑