Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,362,045	T→C	intergenic (‑111/+506)	cadC ← / ← pheU	DNA‑binding transcriptional activator CadC/tRNA‑Phe

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,362,045	0	T	C	100.0%	113.0 / NA	30	intergenic (‑111/+506)	cadC/pheU	DNA‑binding transcriptional activator CadC/tRNA‑Phe
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/13);  total (17/13)

CGCCACTGTGTTGAGCAAAGAAAACCAGAAGATCGATTAATCTCGGCTCAAGGGTAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTTTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACC  >  NC_000913/4361799‑4362289                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     cGCCACTGTGTTGAGCAAAGAAAACCAGAAGATCGATTAATCTCGGCTCAAGGGTAAGTTGACGCTCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTAc                                                                                                                                                                                                                                                  >  1:388331/1‑251 (MQ=255)   ccACTGTGTTGAGCAAAGAAAACAAGAAGATCGATTAATCTCGGCTCAAGGGTAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTAGAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGAAAGACGTTATTCTTAcc                                                                                                                                                                                                                                                 >  2:178781/1‑250 (MQ=255)             gAGCAAAGAAAACCAGAAGATCGATTAATCTCGGCTCAAGGGTAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAAc                                                                                                                                                                                                                                       <  1:38921/250‑1 (MQ=255)                 aaaGAAAACCAGAAGATCGATTAATCTCGGCTCAAGGGTAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAActtctt                                                                                                                                                                                                                                  <  1:27337/251‑1 (MQ=255)                  aaGAAAAACAGAAGATCGATTCATCTCGGCTCAAGGGTAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTg                                                                                                                                                                                                                                 >  1:364836/1‑251 (MQ=255)                                aTCGATTAATCTCGGCTCAAGGGTAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTAtt                                                                                                                                                                                                                    >  2:330124/1‑250 (MQ=255)                                                  aaGGGTAAGTTCAAGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACCAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTgcg                                                                                                                                                                                                 >  2:184056/1‑249 (MQ=255)                                                          gTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAAAAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGaa                                                                                                                                                                                         <  2:388331/251‑1 (MQ=255)                                                                  ccATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATAtt                                                                                                                                                                                 >  1:98160/1‑251 (MQ=255)                                                                         ggCTAATTTGGCTTATGGACGGAGTCACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTAcc                                                                                                                                                                          >  1:381557/1‑251 (MQ=255)                                                                             aaTTTGGGTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATGGCCACAACCTATTTTGACCATCTATAGATGGGTTTATTTACATATTATTGTTGAATGCAAGACGTTATTCTTACCAGCCATAAGCTTCTTGATTACATATGATTACGAAATGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCa                                                                                                                                                                      <  1:184056/251‑1 (MQ=255)                                                                                        tGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTcatcat                                                                                                                                                           >  2:321388/1‑251 (MQ=255)                                                                                                  aaCAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCaaa                                                                                                                                                 <  1:159847/251‑1 (MQ=255)                                                                                                                           aCAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATGTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCAt                                                                                                                        <  2:46218/251‑1 (MQ=255)                                                                                                                               gttgttGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGa                                                                                                                     >  1:341924/1‑250 (MQ=255)                                                                                                                                              aaaCTCATTCGAAAAGGGAAGGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCAc                                                                                                     <  2:382097/251‑1 (MQ=255)                                                                                                                                               aaCTCATTCGAAAAGGGAATGATGCAATGAGAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACt                                                                                                    >  1:267304/1‑251 (MQ=255)                                                                                                                                                         aaaaGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAATTTGTCCGGAGCACTTACTATTTTa                                                                                          <  1:297009/251‑1 (MQ=255)                                                                                                                                                                aaTGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGTTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGAtt                                                                                   <  1:4502/251‑1 (MQ=255)                                                                                                                                                                        aaTGATAATTGCCACAACCTCTTTTTACCCTCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCt                                                                           >  1:322595/1‑251 (MQ=255)                                                                                                                                                                                    cacaTCCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCaa                                                               <  2:130130/251‑1 (MQ=255)                                                                                                                                                                                            aTTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGCAGTTATTCTTACCAGCCATAAACTTCTTGATTACATAATATTACGAAAGGATTTTACTGAGAACCAGCAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTg                                                       >  1:275907/1‑251 (MQ=255)                                                                                                                                                                                            aTTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAAc                                                         <  2:381557/249‑1 (MQ=255)                                                                                                                                                                                                         ataGATGGGTTTATTTACATATTATTTGTTAATGCAAAACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAAGCAGAAGTAATATTCCTTACCAGCAAAATTCATCAGCTTTGCCAAAGAAAAATGTTCAGAAAATAAGCCATGGAAAATGGTCCTTAGCACTTACTGATTTAAGGGATTGTTATTCTTTGCGTGAGCAGGCGAGCGGATACATTtct                                             <  2:416744/248‑1 (MQ=255)                                                                                                                                                                                                         ataGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGACGTAATATTCCTTAACATCAAAATTCATCATCTTTGCAAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGCGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGAAAGCGAACTGATACATTTCTCtt                                          >  2:32836/1‑251 (MQ=255)                                                                                                                                                                                                           aGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCttt                                         <  2:207879/250‑1 (MQ=255)                                                                                                                                                                                                                       ttaCATATTATTGGTGAATGCAAGACGTTATTCTTACCCGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTc                               >  1:436188/1‑248 (MQ=255)                                                                                                                                                                                                                           atatTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAAt                        >  2:139388/1‑251 (MQ=255)                                                                                                                                                                                                                                     tGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTc              >  1:132734/1‑251 (MQ=255)                                                                                                                                                                                                                                                 gTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGAcc  >  1:278525/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     CGCCACTGTGTTGAGCAAAGAAAACCAGAAGATCGATTAATCTCGGCTCAAGGGTAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTTTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACC  >  NC_000913/4361799‑4362289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑