Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,279,031	A→G	I185V (ATT→GTT)	ghxP →	guanine/hypoxanthine transporter GhxP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,279,031	0	A	G	100.0%	100.8 / NA	31	I185V (ATT→GTT)	ghxP	guanine/hypoxanthine transporter GhxP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/16);  total (15/16)

ATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGA  >  NC_000913/4278791‑4279274                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    atTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTTATGGTCTGCCCGTTGCGCTGGGTGACTTCTCTACCTTCCCGGTGGTTATGTCAc                                                                                                                                                                                                                                            <  2:283899/250‑1 (MQ=255)      cgttccTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTGCTTCCACGGGTATCCGTAGCTGGATGTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTGCCCGGTGGTTATGTCACTGGTAg                                                                                                                                                                                                                                      <  2:319416/247‑1 (MQ=255)      cGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTTTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAg                                                                                                                                                                                                                                      <  2:382205/251‑1 (MQ=255)            tGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCat                                                                                                                                                                                                                                 >  1:141709‑M1/1‑248 (MQ=255)               cgcgCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTTGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacct                                                                                                                                                                                                                             <  1:107373‑M1/251‑7 (MQ=255)                  gcTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaac                                                                                                                                                                                                                          <  1:403745‑M1/251‑10 (MQ=255)                  gcTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGCTAATTTCTGCCACGGGTATACGTAGCTGGATTTTGCGCAACTTGCCTCACGGAGCCGCGCTCGGCACGGGGATTGCTATCGGTCCGTTCCTGCTGCTCATTGCCGCTAACGGCGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCCGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGGCatacctaac                                                                                                                                                                                                                          >  1:427514‑M1/1‑242 (MQ=255)                            gtgtTCCTGATGGGTGTCATGTCTTCGGTACACTCTGTCTCGGGTATCCGTCGCTGGTTTTTGCTCACCTCGCCTCCCGGTGTGGCGCGCGGCACGGTGATTGGTATCGGTCTATTCCTGCTGCTCATTGCCTCTTCCGGTGTCGGTTTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTGGCGCTGGGTTAGTTCGCGACTTTCTCGGTGGCTATGTCACTGGTAGGTcatccccaacgacaacaaga                                                                                                                                                                                                                <  2:249542/251‑20 (MQ=255)                               ttCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGGGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaaaaggaaa                                                                                                                                                                                                              >  1:347398‑M1/1‑229 (MQ=255)                                              cTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCACATTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaa                                                                                                                                                                                               >  1:406757‑M1/1‑214 (MQ=255)                                                   tACGGTAATTTCTGCCACGGGTATCCGTCGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggt                                                                                                                                                                                          >  2:294495‑M1/1‑209 (MQ=255)                                                    aCGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACCTGCCTCACGGTGTGGCGCACCACACGGAGATTGGTATCGGTCTGTTCCTGCTGCTCATTACCGCTAACCGTCTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaacaaaccatacccaaacggtcg                                                                                                                                                                                        >  1:184151‑M1/1‑208 (MQ=255)                                                                gCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtt                                                                                                                                                                               <  2:235092‑M1/248‑53 (MQ=255)                                                                           ccGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactgg                                                                                                                                                                  <  1:294778‑M1/250‑66 (MQ=255)                                                                                    gATTTTGCGCAACTTGCCTCACGGGGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCGGCCCGTTGCGCTGGGTGACTGCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttca                                                                                                                                                        <  1:294495‑M1/251‑76 (MQ=255)                                                                                         tGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtg                                                                                                                                                    <  1:247119‑M1/250‑80 (MQ=255)                                                                                                       cACGGGGTGGCGCACGGCACGGGGATTGGTTTCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaagcgtttgcttttaactggggattgttgaggtggcgttgcggtgtt                                                                                                                                       <  2:421638‑M1/249‑93 (MQ=255)                                                                                                         cGGTGTGGCGAACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccacaaggtagtgaatcgtttgcttttaactggggattgatccgggggcgttgcggtgttttg                                                                                                                                    >  2:342306‑M1/1‑155 (MQ=255)                                                                                                                  gcacggcacgGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgtta                                                                                                                             >  1:186464‑M1/1‑146 (MQ=255)                                                                                                                         acgaggattgGTATCGGGCTGTTCCTTCCTCTCATTGCCGCTTACGGTGTCGGTCTTGTTATTAAAACCCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtggg                                                                                                                   <  1:364420‑M1/247‑113 (MQ=255)                                                                                                                                   gTATCGGTCTGTTCCTGCTGCTCATTGCCGCTCACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgactgttaagactgtgggagggagaatc                                                                                                         >  2:268794‑M1/1‑129 (MQ=255)                                                                                                                                   gTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTGCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgtttttaatgttaagactgtgggagggagaatc                                                                                                         <  1:172298‑M1/251‑123 (MQ=255)                                                                                                                                     aTCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctg                                                                                                       <  1:448693‑M1/251‑125 (MQ=255)                                                                                                                                                         cATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCAc                                                                                                                                                                                                                                            <  2:440367/98‑1 (MQ=255)                                                                                                                                                         cATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCACGGTGGTTATGTCAc                                                                                                                                                                                                                                            >  1:440367/1‑98 (MQ=255)                                                                                                                                                                    aCGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTGGCGCTGGGTGACTGCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCataccgaacggcaacaggaaaaaagaccagacccaaaaggtagtgaatcgtttgcttggaacgggggagtgttcaggtggcgttgcggtgttttgaatgttaagac                                                                                                                         <  1:453734‑M1/202‑107 (MQ=255)                                                                                                                                                                       gtgtCGGTCTGGTGATTAAAAACCCGCTTTATGGTCTGCCCGGTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccataaccaacaggaagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggaaacgcatcttgtataggggggggggg                                                                     >  2:353925‑M1/1‑93 (MQ=255)                                                                                                                                                                              tCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggacaaaaaaccatacccaaaaggtagtgcatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggaaccgcctctggtatagggggaggcgaagatagc                                                              >  1:402822‑M1/1‑86 (MQ=255)                                                                                                                                                                                                                           tgacttcgcgACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggaaccgcctctggtatagggggaggcgaagatagcattatttcctctccctcttcttcgctgtaccagataaccggcacc                 >  1:75915‑M1/5‑41 (MQ=255)                                                                                                                                                                                                                                    gACCTTCCCGGTGGTTATGTCACTGGTAGCTCatacctaaccacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggaaccgactctgggatagggggaggcgaagatagcattatttcctctccctcttcttcgctgtaccagataaccggcaccattaattt         >  1:384328‑M1/1‑32 (MQ=255)                                                                                                                                                                                                                                          cccGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggaaccgcctctgggatagggggaggggaagatagcattatttcctctccctcttcttcgctgaaccagataaccggcaccatttatttatagccc  >  2:180018‑M1/1‑26 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    ATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGA  >  NC_000913/4278791‑4279274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑