Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,174,041	A→T	E320D (GAA→GAT)	birA →	DNA‑binding transcriptional repressor/biotin‑[acetyl‑CoA‑carboxylase] ligase BirA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,174,041	0	A	T	100.0%	118.1 / NA	34	E320D (GAA→GAT)	birA	DNA‑binding transcriptional repressor/biotin‑[acetyl‑CoA‑carboxylase] ligase BirA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (18/16);  total (18/16)

CGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATGAAAATAGGA  >  NC_000913/4173811‑4174258                                                                                                                                                                                                                                       |                                                                                                                                                                                                                          cGTGAATTACGTGATGCGCTGGAACTCTTCGACCAAGAAGGCTTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCACAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGCATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTAtt                                                                                                                                                                                                       >  2:204018/1‑251 (MQ=255)                        aCTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCAGAGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCg                                                                                                                                                                                >  1:434738/1‑251 (MQ=255)                         ctctTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGt                                                                                                                                                                               >  1:53590/1‑251 (MQ=255)                              cGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACATGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTc                                                                                                                                                                           <  1:38714/250‑1 (MQ=255)                               gAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTg                                                                                                                                                                         >  1:429997/1‑251 (MQ=255)                                   aagaagGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGAAAATCTTATTAATCGCCCAGTGAACCTTATCCTTGGAGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGCATTTCTTACTTGAGCAGCACGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCCGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAACTTATTTGCGTAGTCTGCCct                                                                                                                                                                     >  1:71016/1‑251 (MQ=255)                                            tGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTAcc                                                                                                                                                             <  1:11313/250‑1 (MQ=255)                                                        tgtcgccacgggAAAAACCGGATAATTTTAATAATCGCCCAGTGAAACTTATGAGAGGAGATAAAGAAATATTTGGCATTTCACGCGGAATAGACACACCGGGGGCTTTATTACTTGAGAAGAATGGAATAATAAAACTCTGGATGGGCGGAGAAATATCCCTGCGTAGTGCAGATAAATCAGAAAGGGGAGTATTAGCTCCCCTGCAAATTATTTGCGTAGTCTGAACTCTTCTAACGCATGATTAgaac                                                                                                                                                >  2:47718/10‑248 (MQ=255)                                                         gTCGCGCTGGGAAAAGCTGGATAATTTTATTAAGCGCCCAGTGAAACTTATCATTGGTGATAAAGAAAGATTTGGCAGTTCACGCGGAATAGACAAACAGGGGGTTTTATTACTTGAGCAGGATGGAATAATAAATCTCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATACAGAAGAAAGGGGAGTAGTCGCTCCCCTGAAAATTATTTGCGTAGTCTGACCTCTTCCACCGCATGATTAGc                                                                                                                                                  <  2:174044/248‑1 (MQ=255)                                                           cgtgcTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGTCCTCTGCTACCGCATGATTAGCACtt                                                                                                                                              <  1:396747/247‑1 (MQ=255)                                                                 gggAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGt                                                                                                                                         <  2:53590/249‑1 (MQ=255)                                                                                        aaTCGCCCAGTGAACCTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGCATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTAtt                                                                                                                                                                                                       >  2:433046/1‑164 (MQ=255)                                                                                        aaTCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTAtt                                                                                                                                                                                                       <  1:433046/164‑1 (MQ=255)                                                                                        aaTCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGa                                                                                                                <  1:219669/251‑1 (MQ=255)                                                                                        aaTCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGAATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCCGGATGGAATAATAAACACCTGGATGGGCGGTGAAATATCCCTGAGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTCTTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACg                                                                                                                 >  1:276275/1‑250 (MQ=255)                                                                                                 gTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAgtaggt                                                                                                          >  1:254753/1‑248 (MQ=255)                                                                                                               tGGTGATAAAGAAATATTTGGTATTTCACGCGGACTAGACAAACCGGGGGCTTTATTACTTGCGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATCTCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAACATGGCGCGATCACGAGTAGGTAGAATAtt                                                                                                  >  2:421695/1‑242 (MQ=255)                                                                                                               tGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGTGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTGCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATAtt                                                                                                  <  1:421695/242‑1 (MQ=255)                                                                                                                      aaaGAAATATTTGGCATTTCACGCGGAATAGACAAAACGGGGGCGTTATTACTTGAGCAGGATGGAATAATAAAACCATTGATTGGCGGTTAAATATCCCTGCGTAGTGCAGATATATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTATGACCTCTTCTACCGCATGATGAGCACTTTACGTCATGATTAAACTGGCGCGCGCATGAGGAGGTAGAATATTTGGCTTTAAGTTCAGc                                                                                  <  2:57124/251‑1 (MQ=255)                                                                                                                               tttGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAGAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGAtc                                                                         >  1:22622/1‑251 (MQ=255)                                                                                                                                  ggCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTATTTAAGAAGGCTGGAATAATAAAACCCTGGATGGGGGGGGAAATATCCCGGAGTAGTGCAGATAAATAAGAAAGGGGAGTATTGGCCCCCCTGAAAATTATTTGCGTGGTTTAACCTCTCCTACCGctt                                                                                                                                                         >  1:314293/1‑166 (MQ=255)                                                                                                                                  ggCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATctct                                                                      <  1:355452/251‑1 (MQ=255)                                                                                                                                    cATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGGCCTCTTCTACCGCAGGATTAGCACGTTTCGTCTGGATTAAACTGGCGGGCTCACGATTATGTAGAATATTGTGCCTTAGGTTCAGCCAGTTGATTTCttt                                                                    <  2:268498/251‑1 (MQ=255)                                                                                                                                              ggAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATAGCCCTGCGTAGTGCAGATAAATAAGAAAGTGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACGGTGCGTCAGGATTAAACTTGCGCGCTCACGAGTAGGTATAATAGTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTc                                                          <  1:209918/251‑1 (MQ=255)                                                                                                                                                   aGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGc                                                     >  1:33388/1‑251 (MQ=255)                                                                                                                                                                    tattaCTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTGTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCAGGGCAGTCGTAATCGcttct                                     <  2:193198/250‑1 (MQ=255)                                                                                                                                                                       taCTTGAGCAGGATGGACTAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTATGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCCCGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGcttcttctt                                 >  2:460503/1‑251 (MQ=255)                                                                                                                                                                             aGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTa                           <  2:121884/251‑1 (MQ=255)                                                                                                                                                                                 ggATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAAttt                       <  2:129268/251‑1 (MQ=255)                                                                                                                                                                                   aTGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTcg                     >  1:53886/1‑251 (MQ=255)                                                                                                                                                                                     ggAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGCAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGACTATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGGCTTAATCGCTTCTTCTTTAGTTCATTTcgcg                   >  2:286710/1‑251 (MQ=255)                                                                                                                                                                                      gAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGt                  <  2:429997/251‑1 (MQ=255)                                                                                                                                                                                                  ccTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATGAAAAt      >  1:447646/1‑251 (MQ=255)                                                                                                                                                                                                       aTGGGAGGTGACATATCCCTGCGGAGTGCAGCTAAATAAGACCGGGGAGTATTCACTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTAACGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGCGTAGGTAGAATATTTTGCTTTTAGTTCAGCCCGTTGATATCTTTCCACAATGTCATGGCCGTCTTCATCGCTTTTTCTTTAGTTAATTTCGGGTAGGTATGCAAATAGGa  >  2:182747/1‑250 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                          CGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATGAAAATAGGA  >  NC_000913/4173811‑4174258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑