Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,123,930	A→T	V176E (GTA→GAA)	cytR ←	DNA‑binding transcriptional repressor CytR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,123,930	0	A	T	100.0%	168.2 / NA	50	V176E (GTA→GAA)	cytR	DNA‑binding transcriptional repressor CytR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (14/36);  total (14/36)

ATCACATCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTACTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTC‑AATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGT  >  NC_000913/4123690‑4124170                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  aTCACATCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGGGACACAGCGGCATCTCTTCGGGACCGGCTATACCGCCCATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCaa                                                                                                                                                                                                                                          >  1:204358/1‑250 (MQ=255)      aTCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGGCACAGCGGCCTCTCTTCGGGGCCGGCTATACAGCCAAACCGTTTATTCCCTTGCTCATATAAATAATTTTTTTCCTCAAATgcgg                                                                                                                                                                                                                                    >  2:352507/1‑251 (MQ=255)          cTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTg                                                                                                                                                                                                                                                <  1:327562/235‑1 (MQ=255)                    agaCAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCAACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGCGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCCATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTTCATCAAATGCGGCGGtgaggttttcg                                                                                                                                                                                                                      >  1:230918/1‑241 (MQ=255)                         gcagTAGGCGGTTGTGGAAAATCAAGCAGCTGCTGCCTCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGGGACACAGCGGCATCTCTTCGGGACCCGCTATACAGCCCATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTTCATTAAATGcggcgggccggttttcggtatt                                                                                                                                                                                                                 >  1:168536/1‑235 (MQ=255)                              aGGCGGTTGTGGAAGATCAAGCATCTGCGGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACt                                                                                                                                                                                                             <  2:342484/250‑1 (MQ=255)                                     tgtgGAAGATCAAGCAGCTGTGGCATCGCTTTGCTTCCGGCTTCGAAGGGGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTGGCAGGCGGTAGTTACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCGTGCTCATATAAAGAATTTTCTGCATCAAATGCGGCGGTCAGATTGGCGATATGAACTTTAGGca                                                                                                                                                                                                      <  2:218760/250‑1 (MQ=255)                                     tgtgGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCCAATGCGGCGGTCAGATTGTCGATATGTACTTTAGGc                                                                                                                                                                                                       >  1:155925/1‑249 (MQ=255)                                          aaGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCCTCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGGCAGATTGTCGCTATGGAATGTTGGcagctccc                                                                                                                                                                                                >  2:104428/1‑250 (MQ=255)                                           aGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCCTCGGGACCGGCTATACAGCCAATCCGTTTATACCCTTGCTCATATAAATAATTTTCTGCATCAAATTCGGCGGGCAGGATGTCGATATTAACTGTTGGcaggctca                                                                                                                                                                                                >  1:260852/1‑245 (MQ=255)                                             aTCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCGTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCGGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGcagctccagct                                                                                                                                                                                             <  1:264165/251‑1 (MQ=255)                                               cAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCagctccagctcc                                                                                                                                                                                           <  1:134952/251‑1 (MQ=255)                                               cAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCCCTTCGGGACCGGCTATACAGCCAAACCGGTTATGCCCTTGCTCATATAAATAATTTTCTGTCTAAAATGCGGCGGGCAGGTTGTGGCTTTTAACTGTAgggcgcgcccgcccc                                                                                                                                                                                           >  2:335059/1‑238 (MQ=255)                                                        gctgcATCGCTTTGCTTCCGGCGTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGAGCAACCATAATGCCGCAGCTACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATGTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCaa                                                                                                                                                                                    <  1:300884/249‑1 (MQ=255)                                                          gtcagcGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAAttc                                                                                                                                                                                <  2:139469/246‑1 (MQ=255)                                                           gcATCGCTTGACTTGCGGCTTAGAAGGGGAAGTCTCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCACTCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAAtt                                                                                                                                                                                 <  2:114396/249‑1 (MQ=255)                                                                          cGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATc                                                                                                                                                                <  1:209932/251‑1 (MQ=255)                                                                           ggCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATcg                                                                                                                                                               <  2:7647/251‑1 (MQ=255)                                                                                                      gATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAAATGTAGGCAGCTCCAGCTCCGGGGCAAAATCGTTTGCCCTCACCATCGGCGGCCGATTTCCCTGTTcccccatgc                                                                                                                                    >  1:115608/1‑244 (MQ=255)                                                                                                             tGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTTTCGATATGAACTGTAGGCAGCGCCAGGTCCGGTGCAAATTCGTGCTGCATCACCATCGGCGGCAGATTACGCTGTTCCTC‑AATGCTGGCAt                                                                                                                             <  1:455897/250‑1 (MQ=255)                                                                                                              gCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCACGCAGGCGGTAGTGACACAGCGGCATCTCTTCGTGACCGGCTATACAGCCAATCCGATTATGCCCTTGCTCATATAAATAATTGTCTCCATCAAATGCGGCGGTCAGATTGGCGATATGAACTGTAGTCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCTTCACCATCGGCGGCAGAGTACGCTGTTCCTC‑AATGCTGGCATCa                                                                                                                           <  1:398361/251‑1 (MQ=255)                                                                                                                 gATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTC‑AATGCTGGCATCAAAc                                                                                                                        <  1:462902/251‑1 (MQ=255)                                                                                                                      aCCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCTGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTC‑AATGCTGGCATCAAACGGCAg                                                                                                                   <  1:255716/251‑1 (MQ=255)                                                                                                                       ccATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGGCAGATTGTCGATATGAACTGTAGGGCGCTCCAGCTCCGGGGCAAAATCGTTCGCCCTCACCCTCGGCGGCAGATTACGCTGTTCCTCCAAT‑CTGGCATTAAACgggcgc                                                                                                                  >  1:297362/1‑247 (MQ=255)                                                                                                                         aTAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTATGCATCAAATGCGGCGGGCAGATTGTCGATATTAAATGTTGGCAAATCCCGCTCCCGTGCAAATTTGTTCGCCATCACCAACGGCGGGCGAATAACCTGTTTCTC‑AATGGTGGCCTCAAAAGGGAGccc                                                                                                                >  1:403048/1‑250 (MQ=255)                                                                                                                           aaTGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTC‑AATGCTGGCATCAAACGGCAGCCtt                                                                                                               <  1:323267/250‑1 (MQ=255)                                                                                                                                      gACGCAGCGCCTGAACATAGCCTGGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTC‑AATGCTGGCATCAAACGGCAGCCTTGAACCCAGcaac                                                                                                   <  2:297362/251‑1 (MQ=255)                                                                                                                                         gCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTC‑AATGCTGGCATCAAACGGCAGCCTTGAACCCAGCaacaac                                                                                                <  2:148001/251‑1 (MQ=255)                                                                                                                                                  gAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTC‑AATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATc                                                                                        <  1:346430/250‑1 (MQ=255)                                                                                                                                                  gAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCAACTCTTCGGGCTCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTC‑AATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCAt                                                                                         <  1:244344/249‑1 (MQ=255)                                                                                                                                                              gCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATGTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTC‑AATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTGGCTTGGt                                                                            <  2:168536/250‑1 (MQ=255)                                                                                                                                                               cAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCGTGCTCATATAAATAATTGTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCGCCAGCTCCGGTGCAAATTCGTTCGTCATCACCATCGGCTGCAGATTACTCTTGTCCTC‑AATGCTGGGATCAATCTTCAGGCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGtga                                                                          <  1:241177/251‑1 (MQ=255)                                                                                                                                                                             acaGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTC‑AATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATTATCAAATCGATa                                                            <  2:62993/251‑1 (MQ=255)                                                                                                                                                                                             gggACCGGCTATACATCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCTGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTC‑AATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTCCctgct                                            <  1:63887/251‑1 (MQ=255)                                                                                                                                                                                                aCCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTC‑AATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGAt                                         <  1:331263/251‑1 (MQ=255)                                                                                                                                                                                                  cGGCTATACAGCCGATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCCGATTGTCGCTCTGAACTGTCGGCAGCTCCAGCTCCGGGGCAAATTCGTTCGCCCTCCCCATCCGCGGCAGGTTACGCTGGTCCTC‑ACTGCTGGCATCAAACGGCAGCCCTGAACCCCGCCACAACCTGCCATCCATTTTCGTGGTGGTGATTCAATCGCTAAAGGGTTTTTCCTTCTTAttt                                       >  2:305361/1‑251 (MQ=255)                                                                                                                                                                                                  cGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTC‑AATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGAttt                                       <  1:352507/251‑1 (MQ=255)                                                                                                                                                                                                    gCTATACAGCCAATCCGTTTAAGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGAGTGTCGATATGAACGTTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCGGTTCCTC‑AAGGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGTTGATGATCAAATCGATAAAGGTATTTTCCTGCTGATTTTg                                     <  2:436519/251‑1 (MQ=255)                                                                                                                                                                                                      tataCAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCACATGCGGCGGTCAGATTGTCGATATGAACTGTAAGACGCTCCAGATCCGGTGCAAATTCGTTCGACATCACCCTCGGCGGCAGATTACGCTGTTCCTC‑AATGCTGGCATCAAACGGCAGCCTTGAACCCAGCCACAAAATGCCATCAATTTGCTTGGTGATGATCAAAACGCTAAAGGTTTTTTCCTGCTGCTTTTGAt                                   >  1:224620/1‑251 (MQ=255)                                                                                                                                                                                                       ataCAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTC‑AATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTGCCTGCTGATTTTGAt                                   <  1:113961/250‑1 (MQ=255)                                                                                                                                                                                                           aGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTC‑AATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGca                              <  1:104428/251‑1 (MQ=255)                                                                                                                                                                                                             ccAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTC‑AATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGcaca                            <  1:18416/251‑1 (MQ=255)                                                                                                                                                                                                               aaTCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTC‑AATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGt                          <  1:188591/251‑1 (MQ=255)                                                                                                                                                                                                                    gTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTACTC‑AATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCa                     <  1:286015/251‑1 (MQ=255)                                                                                                                                                                                                                     tttATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGTCCACTCCAGCTCCGGTGCAAATTCGTTCGCCCTCACCATCGGCGGCAGATTTCGCTGTTCCTC‑AATTCTGGCATCAAAAGGCAGCCCTGCACCCAGCACCAACATGCCATCACTTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATgcgccccgtccccc                     >  2:179046/1‑240 (MQ=255)                                                                                                                                                                                                                       tATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCATCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCTGCAGATTACGCTGTTCCTC‑AATGCTGGCAGCAAACGGCAGCCTTGAACCCAGCAACAACATTCCATCAATTTGCATGTTGATGATCAAATCGATAAAGGTTTTTTCctg                                              <  2:307460/223‑1 (MQ=255)                                                                                                                                                                                                                          gCCCTTGCTCATATAAATAAGTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTC‑AATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTGTTTCCTGCTGATTTTGATGCTCACAGTCGCCAATCAg                <  1:382924/250‑1 (MQ=255)                                                                                                                                                                                                                            ccTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTC‑AATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCAc             <  1:137481/251‑1 (MQ=255)                                                                                                                                                                                                                                   cATATAAATAATTTTCGGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTC‑AATGCTGGCAGCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGat        <  1:229407/249‑1 (MQ=255)                                                                                                                                                                                                                                        aaataaTTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTC‑AATGCGGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAAGCGATAAAGGTTGTGTCCGGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGt  <  1:459026/250‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  ATCACATCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTACTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTC‑AATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGT  >  NC_000913/4123690‑4124170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑