Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,725,393	G→A	intergenic (‑75/+20)	glyQ ← / ← ysaB	glycine‑‑tRNA ligase subunit alpha/putative lipoprotein YsaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,725,393	0	G	A	100.0%	165.2 / NA	46	intergenic (‑75/+20)	glyQ/ysaB	glycine‑‑tRNA ligase subunit alpha/putative lipoprotein YsaB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/23);  total (23/23)

ATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTT  >  NC_000913/3725158‑3725637                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     aTCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAAAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGAAGCCCTGGCGAGCCCAGTAATCCTGTCAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGc                                                                                                                                                                                                                                        >  1:317020/1‑250 (MQ=255)              ccAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCAt                                                                                                                                                                                                                          <  2:57780/251‑1 (MQ=255)              ccAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAAGGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAATCTTTTGCATATTATTTCGTGCTGGATACGTGTGGGTGTATAGGTAGTGATCAGTATACCCGCGGGAGTGATGATAGACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCAt                                                                                                                                                                                                                          <  1:23206/251‑1 (MQ=255)                      cgcAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCGGGCGAGCCCAGTAATCCTGTAATGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTTCAAAACGGGAAAAAGGAGGGCTTGACGCATGGAAAGAt                                                                                                                                                                                                                  <  2:195547/251‑1 (MQ=255)                            cAGGTCCTTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCaaa                                                                                                                                                                                                             >  2:390183/1‑250 (MQ=255)                             aGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCaaaa                                                                                                                                                                                                            >  1:4364/1‑250 (MQ=255)                              ggTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCaaaaa                                                                                                                                                                                                           >  2:349020/1‑250 (MQ=255)                                                 cccGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCaac                                                                                                                                                                                         >  2:90548/1‑248 (MQ=255)                                                     cgcCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGCGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAAc                                                                                                                                                                                    >  1:327827/1‑250 (MQ=255)                                                                aTGTACAATGGGTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACCTTTGCATATTACTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGCGCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAATGCATGGAAAGATGTAAAAAATGGTCATCCGCGTCAAAAGAACAGACAAACt                                                                                                                                                                           <  2:222754/248‑1 (MQ=255)                                                                    ccAATGGTTGAACAATGGTGCAGCCATGGCGAGCCCAGTAATCCCATAAGGTCAGGATCAAGCCCTGGAAGAAACTGGTATCAAACTTTTGAATATAATCTCGTGCTGGATACGTGCGGATTTAAAGGACGGGATCAGTATACCCGCTGGACGGAAGATCTACAGTACAAAACGGGCCAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCttt                                                                                                                                                                    >  1:235132/1‑251 (MQ=255)                                                                      aaTGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGTGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTAc                                                                                                                                                                  <  1:65460/251‑1 (MQ=255)                                                                          gTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGaa                                                                                                                                                                                                                                             <  1:335680/172‑1 (MQ=255)                                                                          gTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGaa                                                                                                                                                                                                                                             >  2:335680/1‑172 (MQ=255)                                                                            tGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATCCAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCGAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAg                                                                                                                                                             <  1:200438/250‑1 (MQ=255)                                                                              aaCAATGGGGCAACCCTGGCGAGCCCAGTAATCCTGGAAGGGCAGGATCAAGCCCTGGAAGGTCCTTGTATCAAACGTTTGCATATTAGTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGGATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAATGCAGGGCTGAACGCATTGAAAGATGCAAAAAATGGCCAGCCGCGTCAAAAGTACAGACaagctagtcgtgaccgaaggt                                                                                                                                                           <  2:418031/250‑20 (MQ=255)                                                                                            ccTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAATCTGCTCTTGACGGAAGGTATAACCGCGCATTTc                                                                                                                                            <  2:327827/251‑1 (MQ=255)                                                                                                 cGAGCACAGCAATCCAGTAAGATCAGGATCACGCCCTGGAAGGCCCTGGTATCCAACTTTCGCATATTCTTTCGTGCTGGATACGCGTGGATTTAAAGGACGGGATCCGTATACCCGCTGGCTGGAAGATATACAGTACAAAACGGGCAACAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCAGCGTCAACCGAACAGACACACTGCTCTTTACGGACGGTCTAACCGCGCATTTCATAGc                                                                                                                                       >  2:26743/1‑251 (MQ=255)                                                                                                  gAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCt                                                                                                                                      >  2:135937/1‑251 (MQ=255)                                                                                                            aTCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAAc                                                                                                                            <  1:349020/251‑1 (MQ=255)                                                                                                                 gTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAGCTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGt                                                                                                                         <  1:261501/249‑1 (MQ=255)                                                                                                                    aGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGaa                                                                                                                    <  2:82696/251‑1 (MQ=255)                                                                                                                    aGGTCAGGATAAAGCCCTGGCAGGCCCTGGTATCACACTTTTGCATATTATTTCGTGCTGGATACGTGTGGCTTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAACAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGCACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCCTAGCTTCCATGGAACTGTTCGa                                                                                                                     >  2:46894/1‑250 (MQ=255)                                                                                                                      gTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAgg                                                                                                                  >  1:42541/1‑251 (MQ=255)                                                                                                                              cAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGAc                                                                                                           <  2:320003/250‑1 (MQ=255)                                                                                                                               aaGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACgg                                                                                                         >  2:250033/1‑250 (MQ=255)                                                                                                                                   ccTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGAt                                                                                                     <  2:4364/251‑1 (MQ=255)                                                                                                                                         aGGTCCTGGCATCAAACTTTTGCATATTATTTCGCGCTGGATACGTGTGGATTTAAAGGCAGGGATCAGTATACCCGCTGGCTGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAACATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTg                                                                                               >  2:178704/1‑251 (MQ=255)                                                                                                                                          ggTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGc                                                                                              >  1:382746/1‑251 (MQ=255)                                                                                                                                                                               ggATACGTGTGGATTTAAAGGACGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTg                                                          >  1:393525/1‑250 (MQ=255)                                                                                                                                                                                     gtgtGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACg                                                   >  2:349369/1‑251 (MQ=255)                                                                                                                                                                                      tgtgGATTTAAAGGAAGGGTTCCGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGc                                                  >  1:364138/1‑251 (MQ=255)                                                                                                                                                                                          gATTTAAAGGAAGGGATCAGAATACCCGCTGGATGGAAGATATCCAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGGGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcg                                              >  1:62269‑M1/1‑247 (MQ=255)                                                                                                                                                                                               aaaGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctc                                         >  1:357161‑M1/1‑242 (MQ=255)                                                                                                                                                                                               aaaGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctc                                         <  1:390183‑M1/251‑10 (MQ=255)                                                                                                                                                                                               aaaGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctc                                         <  1:414654‑M1/251‑10 (MQ=255)                                                                                                                                                                                                aaggaaggGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTg                                                                                                                                <  2:176118/163‑1 (MQ=255)                                                                                                                                                                                                aaggaaggGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTg                                                                                                                                >  1:176118/1‑163 (MQ=255)                                                                                                                                                                                                  ggaaggGATCAGTATACACGCTGGCTGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcgg                                       >  2:420871‑M1/1‑239 (MQ=255)                                                                                                                                                                                                     aggGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTAGAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattc                                   <  1:420871‑M1/251‑16 (MQ=255)                                                                                                                                                                                                               ataCCCGCTGGATGGAAGATAGACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCAGGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGAGTTTTTGCTCGCCGGTGTTATAACGTTTTGCTTCCTGATCCTTGACTTTCGCatcggcctcggattcacgtttatc                          <  2:60456‑M1/250‑25 (MQ=255)                                                                                                                                                                                                                              aaGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATATCCGCGCATTTCATAGCTTCCCTGGAACGGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTAGAACGTTTTGCCGCTTGATCCTTGAATAACGCagcggcctcggattcacgtttagcagggttttttgtgggc          <  2:317020‑M1/251‑41 (MQ=255)                                                                                                                                                                                                                                 atatACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctgg       <  2:382746‑M1/251‑44 (MQ=255)                                                                                                                                                                                                                                      cAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagcc  <  1:138131‑M1/251‑49 (MQ=255)                                                                                                                                                                                                                                       aGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgttt                <  1:134077‑M1/236‑35 (MQ=255)                                                                                                                                                                                                                                       aGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAAATGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgttt                >  2:134077‑M1/1‑202 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     ATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTT  >  NC_000913/3725158‑3725637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑