Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,474,425	T→C	K43R (AAA→AGA)	rpsL ←	30S ribosomal subunit protein S12

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,474,425	0	T	C	100.0%	248.6 / NA	66	K43R (AAA→AGA)	rpsL	30S ribosomal subunit protein S12
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/39);  total (27/39)

CTTAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACG‑CCGGAGCAGTCAAGCGCACCACGTACGG‑TGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGTCAAGAAATATACAACGA  >  NC_000913/3474184‑3474669                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     cTTAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACG‑CCGGAGCAGTCAAGCGCACCACGTACGG‑TGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTgg                                                                                                                                                                                                                                             >  1:115457/1‑251 (MQ=255)     ggACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACG‑CCGGAGCAGTCAAGCGCACCACGTACGG‑TGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGgtagt                                                                                                                                                                                                                                         >  1:42701/1‑251 (MQ=255)         gCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTCACG‑CCGGAGCAGTCAAGCGCACCACGTACGG‑TGTGGTAACGCACACCCGGGAGGTCTTTCACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTCGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTCGtatat                                                                                                                                                                                                                                     >  2:283502/1‑251 (MQ=255)            tCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACG‑CCGGAGCAGTCAAGCGCACCACGTACGG‑TGTGGTAACGAACACCCGGGAGTTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGtatata                                                                                                                                                                                                                                    <  2:115457/249‑1 (MQ=255)                         ggAACGAGCCTGCTTACGTTCTTTAACG‑CCGGAGCAGTCAAGCGCCCCACGTACGT‑TGGGGTAACAAACACCCGGGAGGTCTTTAACACGTCCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGACAGACGAACACGGCATACTTTACGCAGCGCGTAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGc                                                                                                                                                                                                                     <  1:208388/251‑1 (MQ=255)                           gaacggccttgttttgggtattAACGGCCGGAGCAGTCAACAGCACCACG‑ACGGGTGTGGTAACGACCCCGGGGGAGGTCTTTACCACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGGTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTGTACGCAGCGCGGAGTGCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGcc                                                                                                                                                                                                                    <  2:262805/231‑1 (MQ=255)                                    gCTTACGGTCTTTAACG‑CCGGAGCAGTCAAGCGCACCACGTACGG‑TGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGttttt                                                                                                                                                                                                            <  1:54182/249‑1 (MQ=255)                                       tACGGTTTTTAACG‑CCGGAGCAGTCAGGCGCGCCACGTACGG‑TGTGGGAACGAACGCCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGGCCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCAGACTGTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCgg                                                                                                                                                                                                        <  1:291849/250‑1 (MQ=255)                                          ggTCTTTAACG‑CCGGAGCAGTCAAGCGCACCACGTACGG‑TGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCAt                                                                                                                                                                                                    >  1:131884/1‑251 (MQ=255)                                             cTTTAACG‑CCGGAGCAGTCAAGCGCACCACGTACGG‑TGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCt                                                                                                                                                                                                 >  1:132248/1‑251 (MQ=255)                                               ttAACG‑CCGGAGCAGTCAAGCGCACCACGTACGG‑TGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCt                                                                                                                                                                                                 <  2:20706/249‑1 (MQ=255)                                                 aaCG‑CCGGCGCAGTCACGCGCACCACGTACGG‑TGTGGTAACGAACAACCGGGAGGTCTTTAACACGCCCGCCACGGATCACGATCACGGAGTGCTCCTGAAGGATGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACAGTTAGTCAGACGAACACGGCATCCTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTcc                                                                                                                                                                                              >  2:399926/1‑250 (MQ=255)                                                  aCG‑CCGGAGCAGTCAAGCGCCACACGTACGG‑TGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTcc                                                                                                                                                                                              >  1:88310/1‑249 (MQ=255)                                                       cGGAGCAGTCAAGCGCACCACGTACGG‑TGTGGTAACGAACCCCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCa                                                                                                                                                                                        <  1:434317/251‑1 (MQ=255)                                                          agcaagcAAGCGCACCACGTACGG‑GGTGGTAACGAACCCCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCTCTTCGAAACCGTTAGGCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGc                                                                                                                                                                                     <  1:201051/245‑1 (MQ=255)                                                            cagccAAGCGCACCACGTACGG‑TGTGGTAACAAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTGCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCAc                                                                                                                                                                                   <  2:167722/247‑1 (MQ=255)                                                                      caccacGTACGG‑GGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTc                                                                                                                                                                         <  2:132248/251‑1 (MQ=255)                                                                        ccacGGACGG‑TGGGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGc                                                                                                                                                                       <  1:290231/251‑1 (MQ=255)                                                                             gaCGG‑TGTGGTAACGAACCCCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACtt                                                                                                                                                                  <  2:131884/250‑1 (MQ=255)                                                                                    gtgGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCCCGGATCCGGATCACGGAGTGCTCATGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCg                                                                                                                                                              >  1:67061/1‑249 (MQ=255)                                                                                     gggTAACGACCCCCCGGCAGGTCTTAACCACGCCCGCCACGGATCAGGATCACGGAGTGCGCCTGCAGGTTGTGACCTTCTCCACCGAGGTAGGAAGGCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCGAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTGGCTTTTCGCATCGTTGCGAGc                                                                                                                                                           <  2:370776/250‑1 (MQ=255)                                                                                                ccacGGGAGCTCTTTACCACGACCTCCACGCATCAGGATCACGGAGTGCGCCTGCAGGTTGTGACCTTCCCCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGCCGAACACGGCATTCTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGc                                                                                                                                                <  2:255827/248‑1 (MQ=255)                                                                                                  ccGGGAGGTCTATAACACGACCGCAACGGATCCAGAACACGGAGGGCTCCTGCAGGTTGCGACCTTCAACAACGACGTAGGAAGTCACTTCGACACCGTTAGTCAGCCGAACACGGCATACTTTACGCAACGCGGAGATGGATTTTCTAGGAGTGGTAGTATAGACACGCGTACATACGCAACGTTTTTGCGGCCATGCTTACAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGCGCACGTggcgtgcgt                                                                                                                                              >  2:221857/1‑244 (MQ=255)                                                                                                     ggAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTAcc                                                                                                                                           >  2:148378/1‑251 (MQ=255)                                                                                                      gcggTCTTTAACACGACCGCCACGGATCATGGTCACGGAGTGCTCCTGCAGGTTGTGACCTTCTCCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCATACGAACACGGCATACTTTACGCAGCGCGGAGTTGTGTTTTCTAGGAGTGGTAGTATAGACACGAGTACATGCGCCACTTTTTTGCGGGCATGCTTCCAGCGCGAGTTTGGTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCa                                                                                                                                          <  1:73386/249‑1 (MQ=255)                                                                                                                     aCCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTGGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTATGCGTACCAGCTGGTTAACTGTTg                                                                                                                           >  2:396487/1‑251 (MQ=255)                                                                                                                     aCCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTCACTGTTg                                                                                                                           >  2:106014/1‑251 (MQ=255)                                                                                                                         ccACGGATCAGGATCACGGAGTGCTCCGGCAGGGTGTGACCTTCACCACCGATGTAGGAAGGCACTTCGCAACCGTTAGACAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCGCGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCAt                                                                                                                       <  2:357561/251‑1 (MQ=255)                                                                                                                               atcaggatcaCGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAAt                                                                                                                  >  1:445746/1‑250 (MQ=255)                                                                                                                                     atcaCGGAGTGCTCCTGCAGGTTGTGACCTGCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAAGAGCTCCt                                                                                                           <  2:300987/251‑1 (MQ=255)                                                                                                                                          ggAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGttt                                                                                                      <  2:59443/251‑1 (MQ=255)                                                                                                                                               gCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGc                                                                                                  <  2:128496/250‑1 (MQ=255)                                                                                                                                                       aGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCGCCTGGTTTTAGCTTTTGCTTc                                                                                         <  2:187489/251‑1 (MQ=255)                                                                                                                                                        ggTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCg                                                                                        >  1:86358/1‑251 (MQ=255)                                                                                                                                                         gTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGt                                                                                       <  1:50112/251‑1 (MQ=255)                                                                                                                                                           tgtgACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACACCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTa                                                                                      >  1:419192/1‑250 (MQ=255)                                                                                                                                                             tgACCTTCACCACCGATGTAGGAAGTCACTTCTAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTaaa                                                                                    <  2:86358/250‑1 (MQ=255)                                                                                                                                                                     cccaccGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTATCTTTTGCTTCGTAAACACGtaata                                                                           <  2:323522/250‑1 (MQ=255)                                                                                                                                                                      ccaccGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGtaata                                                                           >  2:381524/1‑250 (MQ=255)                                                                                                                                                                         ccGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAAc                                                                       >  1:185872/1‑251 (MQ=255)                                                                                                                                                                          cGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACg                                                                      >  1:106007/1‑251 (MQ=255)                                                                                                                                                                          cGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGGTCGGTTTTCTAGGAGTGGTAGTATAGACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTGTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCAGTAAAGAGCGCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACg                                                                      <  2:42701/251‑1 (MQ=255)                                                                                                                                                                            aTGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTc                                                                    >  2:26242/1‑251 (MQ=255)                                                                                                                                                                            aTGTAGGAAGGCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCTGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAGCTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTc                                                                    <  2:362109/251‑1 (MQ=255)                                                                                                                                                                              gTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCt                                                                  >  1:433934/1‑251 (MQ=255)                                                                                                                                                                                       cACTTCGAAACGGTTAGTCGGACGAACACGGCATACTTTACGCAGCGAGGTGTTAGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATAc                                                                                                                                                                                                                       >  2:313144/1‑93 (MQ=255)                                                                                                                                                                                       cACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGTAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATAc                                                                                                                                                                                                                       <  1:313144/93‑1 (MQ=255)                                                                                                                                                                                            cGAAACCGTTAGTCAGACGAACACGGCATACGTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATGCACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGGGCAGGCCCGTTGCTTTTCGCAACTTTGCGATCACGTGGTTTGCGTACCTGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGGTTTAGCTTGTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAg                                                     <  1:71185/250‑1 (MQ=255)                                                                                                                                                                                              aaaCCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGAc                                                  <  1:106014/251‑1 (MQ=255)                                                                                                                                                                                               aaCCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACg                                                 <  1:148378/251‑1 (MQ=255)                                                                                                                                                                                                 ccGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTTGTTAACTGTTGCCATTAAATAGCTCCTGGTTGTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCGCACACAATATGAGGACGcc                                               <  2:449705/251‑1 (MQ=255)                                                                                                                                                                                                 ccGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGcc                                               <  2:106007/251‑1 (MQ=255)                                                                                                                                                                                                      aGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTGCGTAAACACGTAATAAAACGTCCTCACACAATGTGAGGACGCCGAAt                                           <  2:67061/250‑1 (MQ=255)                                                                                                                                                                                                         cAGACGAACGCGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTGTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCATCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGGAAACACGtaa                                                                             <  2:387022/213‑1 (MQ=255)                                                                                                                                                                                                         cAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGtaa                                                                             >  1:387022/1‑213 (MQ=255)                                                                                                                                                                                                          aGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAAtttt                                        >  2:245417/1‑249 (MQ=255)                                                                                                                                                                                                              gAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGAAGCCGAATTTTAGGGCg                                  >  1:161270/1‑251 (MQ=255)                                                                                                                                                                                                               aaCACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCa                                                                                                                                          <  2:40961/146‑1 (MQ=255)                                                                                                                                                                                                               aaCACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCa                                                                                                                                          >  1:40961/1‑146 (MQ=255)                                                                                                                                                                                                                   cGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGCCGCCGAATTTTAGGGCGATGcc                             <  1:251931/251‑1 (MQ=255)                                                                                                                                                                                                                    ggCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCg                            >  1:11475/1‑251 (MQ=255)                                                                                                                                                                                                                            ttACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCATGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGgt                     <  1:245417/250‑1 (MQ=255)                                                                                                                                                                                                                            ttACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACAGACGCCACGTTTTTGCGGGCATGCGTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACTCAATATGAGGACGCCGAATTTTATGTCGATGCCGAAAAggg                     <  2:268221/250‑2 (MQ=255)                                                                                                                                                                                                                                       ggAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGTCAAGAAatat         >  2:390224/1‑251 (MQ=255)                                                                                                                                                                                                                                             cGGTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCGCGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCGGGTTTGAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAAGTTTAGGGCGATGCCGAAAAGGTGTCAAGAAATATACAACg   <  2:121314/251‑1 (MQ=255)                                                                                                                                                                                                                                              ggTTTTCTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAAGATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGTCAAGAAATATACAACGa  <  1:170381/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     CTTAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACG‑CCGGAGCAGTCAAGCGCACCACGTACGG‑TGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGTCAAGAAATATACAACGA  >  NC_000913/3474184‑3474669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑