Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,449,779	G→A	S35S (AGC→AGT)	rpsC ←	30S ribosomal subunit protein S3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,449,779	0	G	A	100.0%	213.1 / NA	60	S35S (AGC→AGT)	rpsC	30S ribosomal subunit protein S3
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/29);  total (31/29)

CCAGTTTTGCGTCCAGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCGCTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACT  >  NC_000913/3449538‑3450024                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      ccAGTTTTGCGTCCAGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTc                                                                                                                                                                                                                                                  <  2:145313/247‑1 (MQ=255) ccAGTTTTGCGTCCAGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTc                                                                                                                                                                                                                                                  >  1:145313/1‑247 (MQ=255)   aGTTTTGCGTCCAGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATTTCCTCTACGACCTTACGCAGTTTTTCTACGTCTTCTCCTTTTTTACCGATAACGATTCCCGGGCGTGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGTACTCTCGATAACGATACGGGATACGGACGCTTTATTCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGtt                                                                                                                                                                                                                                            <  1:234989/251‑1 (MQ=255)                     ggCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTGTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACTCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGgt                                                                                                                                                                                                                          <  2:60966/251‑1 (MQ=255)                      gCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTTCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGgtg                                                                                                                                                                                                                         >  1:455709/1‑251 (MQ=255)                        ttACGAACTTCGGCGTTGTTGATCTGTGCCGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCCCCTTTTTTACCGCTAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGCTAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCCGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGtt                                                                                                                                                                                                                       >  2:237840/1‑251 (MQ=255)                          aCGAACTTCGGCGATGTTGATCTGTGCAAGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCGTTTTTACCTATAACGATACCCGGGCGAGCAGTGGGAATGGTTACACGGATGCTCTTAGCCGGACTCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTATTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCTAATTCTTTGGTGTTc                                                                                                                                                                                                                      <  2:100045/250‑1 (MQ=255)                               cTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAAc                                                                                                                                                                                                                >  2:31274/1‑251 (MQ=255)                                  cGGCGATGTTGCTCTGTGCAGGAAAGCTAGCGATGTACGCTACGACCTTAAGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATACCGATACCCGGGCGCGCAGTGTGACTGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGAAAAcc                                                                                                                                                                                                               >  2:286555/1‑249 (MQ=255)                                                     aGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTa                                                                                                                                                                                          <  2:109573/251‑1 (MQ=255)                                                           gCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATAc                                                                                                                                                                                    <  1:67126/251‑1 (MQ=255)                                                             cAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTGGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATAccc                                                                                                                                                                                  <  1:70288/251‑1 (MQ=255)                                                              aGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAACCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCACACCAGGTAGAGTTCCATGGTTTTACAATAccc                                                                                                                                                                                  >  2:400221/1‑250 (MQ=255)                                                                cGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAgg                                                                                                                                                                               >  1:356515/1‑251 (MQ=255)                                                                cGATGTCCGCTACGACCGTCCGCAGTTTGTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTGACACGGATGCTCTTAGCCGGACGCTAGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAATCCAGGTAGAGTTCCATGGTGTTACAATGCCCAgg                                                                                                                                                                               <  2:200792/251‑1 (MQ=255)                                                                      ccGCTACGACCTTACGCAGTTTTTCTACGTCTTCACATTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGact                                                                                                                                                                          >  2:278433/1‑248 (MQ=255)                                                                        gCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTGTACCGATAGCGATTCCCGGGCGAGAAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCGTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATAcc                                                                                                                                                                       <  2:187892/251‑1 (MQ=255)                                                                           aCGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCAt                                                                                                                                                                     <  1:420/250‑1 (MQ=255)                                                                                      cAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACtt                                                                                                                                                         <  1:144842/251‑1 (MQ=255)                                                                                      cAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTGTTACAATACCCAGGCGAATACCATTAGGATGTACtt                                                                                                                                                         <  2:239069/251‑1 (MQ=255)                                                                                           tttCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTg                                                                                                                                                     >  1:18566/1‑250 (MQ=255)                                                                                                   tCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGc                                                                                                                                            >  1:371594/1‑251 (MQ=255)                                                                                                                 aCCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGtc                                                                                                                              <  2:329944/251‑1 (MQ=255)                                                                                                                       aaCGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGACTACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCg                                                                                                                        >  1:416251/1‑251 (MQ=255)                                                                                                                       aaCGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCg                                                                                                                        >  1:222340/1‑251 (MQ=255)                                                                                                                                 gggCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTGGGTGTTCGCAAACCAGGTAGAGTTCCATGGTGTTGCAATACCCAGGCGAATACCATTATTATGTACGGTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGAcaca                                                                                                              <  1:286555/251‑1 (MQ=255)                                                                                                                                     gAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGc                                                                                                                                            <  2:114535/217‑1 (MQ=255)                                                                                                                                     gAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGc                                                                                                                                            >  1:114535/1‑217 (MQ=255)                                                                                                                                      aGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGCTACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTa                                                                                                                                                            >  1:264820/1‑200 (MQ=255)                                                                                                                                      aGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACGTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTa                                                                                                                                                            <  2:264820/200‑1 (MQ=255)                                                                                                                                       gCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAACTCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCATCGATCGGACACAAccccc                                                                                                        >  2:206401/1‑248 (MQ=255)                                                                                                                                       gCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCAt                                                                                                                                                                     <  2:3420/190‑1 (MQ=255)                                                                                                                                       gCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCAt                                                                                                                                                                     >  1:3420/1‑190 (MQ=255)                                                                                                                                                  ggTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCt                                                                                             >  1:253904/1‑251 (MQ=255)                                                                                                                                                       cacGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTgc                                                                                        >  1:100534/1‑251 (MQ=255)                                                                                                                                                        acGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTgcg                                                                                       >  1:46493/1‑251 (MQ=255)                                                                                                                                                            aTGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGATGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGAGGCTGGTGCGCTTc                                                                                   >  1:442752/1‑251 (MQ=255)                                                                                                                                                                        ggACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCg                                                                                                                    <  1:215081/206‑1 (MQ=255)                                                                                                                                                                        ggACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCg                                                                                                                    >  2:215081/1‑206 (MQ=255)                                                                                                                                                                               cGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGCATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGGGCTTCAGGATGCGATCTGCACGAc                                                                >  2:360360/1‑251 (MQ=255)                                                                                                                                                                                gATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGAcc                                                               <  2:18566/251‑1 (MQ=255)                                                                                                                                                                                 aTAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCt                                                              <  2:455709/251‑1 (MQ=255)                                                                                                                                                                                   aaCGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCtt                                                             <  1:117144/250‑1 (MQ=255)                                                                                                                                                                                     cGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCACGGTTGTCAGCGACTTCTTTGGTGTTCGCAACCCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTCCTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACCGTGATGTGGCTGGGGCGCTTCAGGATGCGATCTGCACGCCCTTTTg                                                          >  1:26559/1‑251 (MQ=255)                                                                                                                                                                                         aCGAGATACGGACGCTGTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCAc                                                       <  1:237840/250‑1 (MQ=255)                                                                                                                                                                                                aCGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCATCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATa                                               <  2:222340/251‑1 (MQ=255)                                                                                                                                                                                                         ttAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCt                                        <  2:46493/249‑1 (MQ=255)                                                                                                                                                                                                           aGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCa                                     <  2:349999/250‑1 (MQ=255)                                                                                                                                                                                                                   ccTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCggg                             <  2:100534/250‑1 (MQ=255)                                                                                                                                                                                                                     ttAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCt                          >  1:306534/1‑251 (MQ=255)                                                                                                                                                                                                                      tAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGACTTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGCTTTACAATACCCAGGCGCATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGGGCTTCATGCTCGGGCCtt                         >  2:228159/1‑251 (MQ=255)                                                                                                                                                                                                                       aGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTc                        <  1:3538/251‑1 (MQ=255)                                                                                                                                                                                                                               aCTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACg                 >  1:71956/1‑250 (MQ=255)                                                                                                                                                                                                                                 tGACGTACTTTAAACTCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGaa               >  2:305178/1‑250 (MQ=255)                                                                                                                                                                                                                                   aCGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGTGCCGTCGTCTACGaaaa             <  2:205816/250‑1 (MQ=255)                                                                                                                                                                                                                                    cGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAAtt           >  1:32163/1‑251 (MQ=255)                                                                                                                                                                                                                                     gTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGCTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATAACCCGGCGACTACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGAACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAAttt          >  1:281096/1‑251 (MQ=255)                                                                                                                                                                                                                                       aCTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGGCTTCGTCTACGAAAAttttt        >  2:58338/1‑250 (MQ=255)                                                                                                                                                                                                                                             aaaTCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACt  <  1:5725/251‑1 (MQ=255)                                                                                                                                                                                                                                             aaaTCACTGTCCAGGTTGCCAGCGGATTCTTTGCTGTTCGCAAACCCGGTAGAGTTCCATGGTTTTACAATACCCAGGCGCATACCATTAGGATGTACTTTCTGACACATTGCTAGTCTCACGAGTCTCAGCGATCGGACACCACCACAGTGATGTGGCTGGGTCGCTTCAGGATGCGATCTGCACGACCTTTTGCAACCGGCATAATGCGCTTCATGCTCGGGCCTACGTCTACGCACATTTTCGTCACt  >  2:441812/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      CCAGTTTTGCGTCCAGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCGCTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACT  >  NC_000913/3449538‑3450024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑