Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,375,5280CA30.8% 54.5 / 12.0 40D220E (GAC→GAA) dcuDputative transporter DcuD
Reads supporting (aligned to +/- strand):  ref base C (22/5);  new base A (0/12);  total (22/18)
Fisher's exact test for biased strand distribution p-value = 1.58e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GATGGTCACCTTATTTCCGACGCTGGTGAGTCTGGGAGTAAGTCGTCTCTCTGC‑GGTGGCAGTTATCGCAACCACGATGTCCATTGAGTGGGGGATTCTGGAAACGAACTCCATTTTTGCTGCCCAGGTAGCGGGAATGAAAATTGCCACATACTTCTTCCACTACCAGCTTCCGGT‑CGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTT‑CGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACG  >  NC_000913/3375291‑3375769
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 
gATGGTCCCCTGATTTCCGACGCTGGGGAGTCTGGGAGTGATTCGTCTCTCTGC‑GGTGGCAGTTATCGCAACCACGATGGCCATTGAGTGGGGGATTCTGGAAACGAACTCCATTTTTGCTGCCCAGGTAGCGGGAATGAAAATTGCCACATACTGCTTCCACTACCAGCTTCCGGT‑CGCCTCTTGCGGCATTATCTCGGTGGCGATCTCCCACCTTTT‑CGTGCAACGCGCTTTTGACAAAAAAGATaa                                                                                                                                                                                                                                        <  2:366297/249‑1 (MQ=255)
   ggTCACCTTATTTCCGACGCTGGTGAGTCTGGGAGTAAGTCGTCTCTCTGC‑GGTGGCAGTTATCGCAACCACGATGTCCATTGAGTGGGGGATTCTGGAAACGAACTCCATTTTTGCTGCCCAGGTAGCGGGAATGAAAATTGCCACATACTTCTTCCACTACCAGCTTCCGGT‑CGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTT‑CGTGCAACGCGCTTTTGACAAAAAAGATAAAAat                                                                                                                                                                                                                                    >  2:364389/1‑250 (MQ=255)
   ggTCACCTTATTTCCGACGCGTGTGAGTCTGGGAGGAAGTCGTTGCTCTTC‑GGTTGCAGTTATCGCAACCACGATGTCCATTGATGGGGGGATGCTGGAAACGAACTTCATTTGTGCTGCCCAGGTAGCGGGAATGAAAATTGCCACATTCTTCTTCCACTGCCAGCTTCCTGT‑CGCCTCTTGCGTCATTATCGCGGTGGCGATCTCCCACTTTTT‑CGTGCAACGCGCTTTTGACAAAAAAGATAAAAat                                                                                                                                                                                                                                    <  2:154243/250‑1 (MQ=255)
            aTTTCCGACGCTGGTGAGTCTGGGAGTAAGTCGTCTCGCTGC‑GGTGGCAGTTATCGCAACCACGATGTCCATTGAGTGGGGGATTCTGGAAACGAACTCCATTTTTGCTGCCCAGGTAGCGGGAATGAAAATTGCCACATACTTCGTCCACTACCAGCTTCCGGT‑CGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTT‑CGGGCAACGCGCTTTTGACAAAAAATATAAAAATATCAATCAc                                                                                                                                                                                                                           <  2:265958/250‑1 (MQ=255)
               cccGACGCTGGTGAGTCTGGGAGGTAGTCGTCTCTCTGCTGGTG‑CAGTTAACGCAACCACGATATTCATTGAGTGGGGGATTCTGGAAACGAACTCCAGTTGTGCTGCCCAGGTAGCGGGAATGAAAATTGCCACATACGTCTTCCGCTACCAGCTTCCGGT‑CGCCTCTGGCGTCATTAGCACGGTGGCGAGCTCCCACTTTTT‑CGTGCAACTCGCTTTGGACAAAAAAGAGAAAAATATCAATCACGAAc                                                                                                                                                                                                                       <  2:314682/250‑1 (MQ=255)
                        ggTGAGTCTGGGAGTAAGTCGTCTCTCTGC‑GGTGGCAGTTATCGCAACCACGATGTCCATTGAGTGGGGGATTCTGGAAACGAACTCCATTTTTGCTGCCCAGGTAGCGGGAATGAAAATTGCCACATACTTCTTCCACTACCAGCTTCCGGT‑CGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTT‑CGTGCAACGCGCTTTTGACAAAAAAGATAAAAAGATCAATCACGAACAGGCagag                                                                                                                                                                                                               <  1:413626/250‑1 (MQ=255)
                                ggggAGTAAGTCGTCTCTCTGC‑GGTGGCAGTTATCGCAACCACGATGTCCATTGAGTGTGGGATTCTGGAAACGAACTCCATTTTTGCTGCCCAGGTAGCGGGAATGAAAATTGCCACATACTTCTTCCACTACCAGCTTCCGGT‑CGCCTCTGGCATGGTGAGCTCGGGGTCGATCGCCCTCTTGTT‑CGTGCAACGCGCTTTTGAGAAAATAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGct                                                                                                                                                                                                      <  1:212550/250‑1 (MQ=255)
                                       aaGTCGTCTCTCTGC‑GGTGGCAGTTATCGCAACCACGATGTCCATTGAGTGGGGGATTCTGGAAACGAACTCCATTTTTGCTGCCCAGGTAGCGGGAATGAAAATTGCCACATACTTCTTCCACTACCAGCTTCCGGT‑CGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTT‑CGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGAt                                                                                                                                                                                                >  1:101643/1‑250 (MQ=255)
                                                       ggTGGCAGTTATCGCAACCACGATGTCCATTGAGTGGGGGATTCTGGAAACGAACTCCATTTTTGCTGCCCAGGTAGCGGGAATGAAAATTGCCACATACTTCTTCCACTACCAGCTTCCGGT‑CGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTT‑CGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGctct                                                                                                                                                                                >  1:53263/1‑251 (MQ=255)
                                                                     cAACCACGATATCCATTGAGTGGGGGATTCTGGAAACGTACTCCATTTATGATGCCCAGGTAACGGGAATAACAATTGCCACATACTTCTTCCACTACCCGCTTCCGGT‑CGCCTCTTGCGTCATCATCTCGGTGGCGATCTCCCCCTTTTT‑CGTGCAACGCGCTTTTGACAAAACAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCAtttt                                                                                                                                                                   >  1:296042/1‑250 (MQ=255)
                                                                           cGATGTCCATTGAGTGGGGGATTCTGGAAACGCCCTCCCTTTTTGCTGCCCAGGTAGCGGGAATGAAAATTGCCACATACTTCTTCCACTACCAGCTTCCGGT‑CGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTT‑CGTGCAACGCGCTTTTGACAAAAAAGATACAAATATCAATCACGAACAGGCAGTGCAAAACGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCtgtg                                                                                                                                                            >  2:220565/1‑251 (MQ=255)
                                                                            gATGTCCATTGAGTGGGGGATTCTGGAAACGAACTCCATTTTTGCTGCCCAGGTAGCGGGAATGAAAATTGCCACATACTTCTTCCACTACCAGCTTCCGGT‑CGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTT‑CGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCtgtg                                                                                                                                                            >  2:329161/1‑250 (MQ=255)
                                                                            gATGTCCATTGAGTGGGGGATACTGGAAACGAACTCCATTTTTGCTGCTCAGGTAGGGGAAATGAAAATTGCCACATCCTTCTCCCACTCCCAGCTTCCGGT‑CGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTG‑CGTGCACCGCGCTTTTGAAAAAAAAGATAAAAATACCAACCACGAACAGGCAGAGCAAAAAGCTCGCTATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGa                                                                                                                                                           <  1:281363/251‑1 (MQ=255)
                                                                               gTCCATTGAGTGGGGGATTCTGGAAACGAACTCCATTTTTGCTGCCCAGGTAGCGGGAATGAAAATTGCCACATACTTCTTCCACTACCAGCTTCCGGT‑CGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTT‑CGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGc                                                                                                                                                        >  1:146304/1‑251 (MQ=255)
                                                                                                    gaaaaCCAACTCCTTTTTCGCTGCCCAGGTAGGGGGAATGAAAATTGCCACATACTTCCCCCCCCCCCAGCTCCCGGT‑CGCCTCTTGCGTCATTATCGCGGTGGCGATCCCCCACTTTTT‑CGTGCAACCCGTTTTTGAAAAAAAAGAAAAAAATACCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATgctcggc                                                                                                                                    <  2:91531/248‑1 (MQ=255)
                                                                                                      aaaCGAACTCCATTTTTGCTGCCCAGGTAGCGGGAATGAAAATTGCCACATACTTCTTCCACTACCAGCTTCCGGT‑CGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTT‑CGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATgctcggctcg                                                                                                                                 >  1:408796/1‑251 (MQ=255)
                                                                                                           aaCTCCATTTTTGCTGCCCAGGTAGCGGGAATGAAAATTACCACATACTTCTTCCACTACCAGCTTCCGGT‑CGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTT‑CGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGtt                                                                                                                            >  1:165997/1‑251 (MQ=255)
                                                                                                            accccttttttGCTGCCCAGGTAGCGGGAATGAAAATGGCCACATACTTCTCCCACTCCCAGCTTCCGGT‑CCCCTCTGGCGTCATTATCGCGGTGGCGATCCCCCCCTTTTT‑CGTGCAACGCGCTTTTGAAAAAAAAAATAAAAATATCAACCACAAACAGGCAGAGAAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTc                                                                                                                           <  1:348650/245‑1 (MQ=255)
                                                                                                                cATTTTTGCTGCCCAGGTAGCGGGAATGAAAATTGCCACATACTTCTTCCACTACCAGCTTCCGGT‑CGCATCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTT‑CGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTCAATGTGATGCCGTTACTCCTGATGCCCGGCTCGATGTTCCTCg                                                                                                                       >  2:233942/1‑251 (MQ=255)
                                                                                                                         ggccacggaaGCGGAAATGAAAATTGCCCCATTCTTCTTCCCCTCCCGGCTTCCGGT‑CGCCTCTTGCGTCTTTATCTGGTTGGCTATCCCCCCCTTTTT‑GTTGCACCCCCTTTTTAAAAAAAAAAATAAAAAAACCACTCAGGACCGGACAGAGAAAAAAGCTCTCGATAATCTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCg                                                                                                              <  1:80285/242‑1 (MQ=255)
                                                                                                                           cccgggtggCGGAAATGAAAATCGCCACATTCTTCTTCCCCCCCCAGCT‑CCGGTTCCCCTCTTGCCCCATTACCTGGGTGGCGACCCCCCACTTTTT‑CTTGCGCCGCGCGCTTGCAAAAAAAAAAAAAAAAATCATCCACGAGCAGGCAAAGAAAAAGGCTCTCAAGAATTTCCCTCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCggg                                                                                                            <  1:35660/243‑1 (MQ=255)
                                                                                                                            ccAGGTAGCGGGAATGAAAATTGCCACATACTTCTTCCACTACCAGCTTCCGGT‑CGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTT‑CGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGc                                                                                                           >  1:62361/1‑251 (MQ=255)
                                                                                                                                          tGAAAATTGCCACATACTTCTTCCACTACCAGCTTCCGGT‑CGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTT‑CGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCCGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCTCCCACGTCGGGCTGATGCAGTccga                                                                                              >  1:253908/1‑247 (MQ=255)
                                                                                                                                           gAAAATTGCCACATACTTTTTCCACTACCAGCTTCCGGT‑CGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTT‑CGTGCCACGCGCTTTTGACAAAAAAGCTAACAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTACCGCCGCTCTATTAAGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTAGGCTCGCTGTTCATCCCCTACGTCGGGCAGATGCAGTccgcac                                                                                            >  2:299850/1‑246 (MQ=255)
                                                                                                                                            aaaaTTGCCACTTCCTTCTCCCACTCCCAGCTTCCGGT‑CGCCTCTTGCGTCATTATCTCGGTGGCGATCCCCCCCTTTTT‑CGTGCAACGCGCTTTTAAAAAAAAAGAAAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACt                                                                                           <  1:364389/251‑1 (MQ=255)
                                                                                                                                            aaaaTTGCCACATACTTCTTCCACTACCAGCTTCCGGT‑CGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTT‑CGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACt                                                                                           >  2:347854/1‑251 (MQ=255)
                                                                                                                                             aaaTTGCCACATACTTCTTCCACTACCAGCTTCCGGT‑CGCATCTTGCGTCATTATCTCGGAGGCGCTCTCCCACTTTTT‑CGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGATCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCCGAACTg                                                                                          >  1:277188/1‑251 (MQ=255)
                                                                                                                                                      caTACTTCTTCCACTCCCAGTTTCCGGT‑CCCCTCTGGCGTCATTAGCTCGGTGGCGATCTCCCATTTTTT‑CGTGCAACGCGTTTTTGAAAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGAAAAAAGCTCTGGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCtggtg                                                                                 <  1:329084/251‑1 (MQ=255)
                                                                                                                                                          cttcttCCACTACCAGCTTCCGGT‑CGCCTCTTGCGTCATTATATCGGTGGCGATCTCCCACTTTTT‑CGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTg                                                                                                                              >  1:77439/1‑202 (MQ=255)
                                                                                                                                                                   cTACCAGCTTCCGGT‑CGCCTCTTGCGTCATTATCTCGGTCGCGATCTCCAACTTTTT‑CGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATACTGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTat                                                                    >  1:222075/1‑250 (MQ=255)
                                                                                                                                                                              cGGT‑CGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTT‑CGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACt                                                         >  1:371739/1‑251 (MQ=255)
                                                                                                                                                                               ggT‑CGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTT‑CGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACtg                                                        >  1:234384/1‑251 (MQ=255)
                                                                                                                                                                                             gTCATTATCTCGGTGGCGATCTCCTACTTTTT‑CGTGCAACGCGCTTTTGCCAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTc                                                                                                               >  2:264099/1‑183 (MQ=255)
                                                                                                                                                                                             gTCATTATCGCGGTGGCGACCCCCCACTTTTT‑CTGGCAACGCGTTTTTGAAAAAAAAAAAAAAAAAATCAACCACGACAAGGCAGAGAAAAAAGCTCTCGAAAATTCCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTc                                                                                                               <  1:264099/183‑1 (MQ=255)
                                                                                                                                                                                                tttaccggggTGGCGATCTCCCTCTTTTC‑CGTGCAACGTGCTTTAGAAAAAAAAGATAAAAATATAAACCACGAACGGGCAAAGAAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTttgttg                                         <  2:62361/242‑1 (MQ=255)
                                                                                                                                                                                                 ttATCTCGGTGGCGATCTCCCACTTTTT‑CGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGCACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTttgttg                                         >  1:28508/1‑249 (MQ=255)
                                                                                                                                                                                                  taccgcGGTGGCGATCTCCC‑CTTTTTGCGTCAAACGCGCTTTGGAAAAAAAAAATAAAAATATCAATCCCAAACAGGCAGAGAAAAAGGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGTCTGTGACGATGTTTGTTGAg                                       <  1:176274/245‑1 (MQ=255)
                                                                                                                                                                                                       cGGTGGCGATCTCCCACTTTGT‑CGTGCAACGCGCTTTTGAAAAAAAAGAAAAAAATATCAACCCCAAACAGGCAGAGAAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGttctt                                  <  1:329161/250‑1 (MQ=255)
                                                                                                                                                                                                                    cctcTTTTT‑CGTGCAACGCGCTTTTGAAAAAAAAAAAAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTAGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACt                    <  1:110697/248‑1 (MQ=255)
                                                                                                                                                                                                                                        gcTTTTGACAAAAAAGATAAACATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGCTGTTTGTTGCGTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACg  >  2:188380/1‑250 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 
GATGGTCACCTTATTTCCGACGCTGGTGAGTCTGGGAGTAAGTCGTCTCTCTGC‑GGTGGCAGTTATCGCAACCACGATGTCCATTGAGTGGGGGATTCTGGAAACGAACTCCATTTTTGCTGCCCAGGTAGCGGGAATGAAAATTGCCACATACTTCTTCCACTACCAGCTTCCGGT‑CGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTT‑CGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACG  >  NC_000913/3375291‑3375769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑