Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,014,2220CA21.7% 99.2 / 10.5 60Q332K (CAA→AAA) fliFflagellar basal‑body MS‑ring and collar protein
Reads supporting (aligned to +/- strand):  ref base C (34/13);  new base A (0/13);  total (34/26)
Fisher's exact test for biased strand distribution p-value = 2.01e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAG‑CTTAATGAGAG‑CGAGCAAA‑GCGGTT‑CC‑GG‑TTATCCGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTC  >  NC_000913/2013973‑2014472
                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                           
aTCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTAGTTCACGCCAG‑CTTAATGAGAG‑CGAGCACA‑GCGGTT‑CC‑GG‑TTATCCGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATc                                                                                                                                                                                                                                                             >  2:136931/1‑250 (MQ=255)
       cGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGGCTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCGCTATCGGTGATGATTCGCAAGCGGCGCTTCGTTCACGCCAG‑CTTGATGAGAG‑CGAGCAAA‑GCGGTT‑CC‑GG‑TTATCCGGG‑CGGCGCACCGGGGGCGTGGT‑C‑GAATCAACTGGCGCCTGCG‑AAGAAC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAAc                                                                                                                                                                                                                                                    <  1:452338/251‑1 (MQ=255)
            aTTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAG‑CTTAATGAGAG‑CGAGCAAA‑GCGGTT‑CC‑GG‑TTATCCGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGcc                                                                                                                                                                                                                                                >  1:94630/1‑250 (MQ=255)
               gttgGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAG‑CTTAATGAGAG‑CGAGCAAA‑GCGGTT‑CC‑GG‑TTATCCGGG‑CGGCGTACCGGGGGCGTGGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCcagc                                                                                                                                                                                                                                             >  1:255881/1‑250 (MQ=255)
                          tAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAG‑CTTAATGAGAG‑CGAGCAAA‑GCGGTT‑CC‑GG‑TTATCCGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAgac                                                                                                                                                                                                                                 >  2:279137/1‑249 (MQ=255)
                          tAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCAAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAG‑CTTAATGAGAG‑CGAGCAAA‑GCGGTT‑CC‑GG‑TTATCCGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGc                                                                                                                                                                                                                                  >  2:52838/1‑250 (MQ=255)
                                     cccgggTTCCGGCGCAGCTGGACTTCGCAAGAAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAACCTCATGCGGCGCTTCGTTCACGCCAG‑CTAAAGGAGGG‑CGAG‑AAA‑GGGGTTTCC‑GG‑TTATCGGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCCCCTGCG‑AAAACC‑GCGCAAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAAt                                                                                                                                                                                                                      <  1:326786/247‑1 (MQ=255)
                                        aGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAG‑CTTAATGAGAG‑CGCGCAAA‑GCGGTT‑CC‑GG‑TTATCCGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATCAC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCCATAGt                                                                                                                                                                                                                   >  2:126707/1‑251 (MQ=255)
                                        aGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAG‑CTTAATGAGAG‑CGAGCAAA‑GCGGTT‑CC‑GG‑TTATCCGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAgg                                                                                                                                                                                                                   >  2:182602/1‑250 (MQ=255)
                                        aGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAG‑CTTAATGAGAG‑CGAGCAAA‑GCGGTT‑CC‑GG‑TTATCCGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGt                                                                                                                                                                                                                   >  1:107389/1‑251 (MQ=255)
                                        aGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAG‑CTTAATGAGAG‑CGAGCAAA‑GCGGTT‑CC‑GG‑TTATCCGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGt                                                                                                                                                                                                                   >  1:359661/1‑251 (MQ=255)
                                         ggTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGCAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAG‑CTTAATGAGAG‑CGAGCAAA‑GCGGTT‑CC‑GG‑TTATCCGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTg                                                                                                                                                                                                                  >  2:347181/1‑251 (MQ=255)
                                            accggcgcggcgGGACTCCGCCAGAAAAGAAAAAACGGAAGAACAGTATCGCCCTAAGGGGGATGAATCCCTGGCGGCGCTTCGTTCACGCCAG‑CTTAAGGAGAG‑CGAGCAAA‑GCGGTT‑CC‑GG‑TGACCCGGG‑CGGCGTCCCGGGGGCGTTGT‑C‑GAAGCAACCGGCAGCTGCA‑AATAAC‑GCGCCAATCAGCCCGCCTCCGGAAAATA‑AAAATATCCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGc                                                                                                                                                                                                               <  1:46778/249‑1 (MQ=255)
                                             aCGGCGCAGCTTGCCTTCGCCAGTAAAGAACAAACGGAAAAACAGTGCCGCCCGAAGGGTGATGAATCTCATGCGGCGCTTCGTCCCCGCCTG‑CTTAATGAGAG‑CGAGCAAA‑GGGGTT‑CC‑GG‑TTATCGGGG‑CGGCGTACCGGGGGCGTGGG‑C‑GAACCACCCGGC‑CCTCCGGAATAAC‑GCGCCAATCAGCCCGCCTCCGGAAAATA‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGcc                                                                                                                                                                                                              <  1:10391/251‑1 (MQ=255)
                                                        ggatgtcgcaagaaaaGAACAAAAGGAAGAACAGTACCGCCCTACCGGTGATGAATCTCATGGGGCGTTTCGTTCACGCCAG‑CTTAATGAGAG‑CGAGCAAG‑GCGGTT‑CC‑GT‑TTATCCGGG‑GGGCGTCCCGGGGGCTTTGT‑C‑GAATCACCCGGCCCCTTCG‑AAAACC‑GCGCCATTCAGCACGCCTCCGGAAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGcaca                                                                                                                                                                                                    <  1:41731/237‑1 (MQ=255)
                                                         gtcTTCGACAGAAAAAAACAAACGAAACAACAGTATCCCCCTAACGGTGATGACTCTCATGCGGTGCTTCGTTCGCGCCAG‑CTTAATGAGAG‑CGAGAAAA‑G‑GGTT‑CCCGG‑TTACCCGGG‑CGGCTTACGGGGGTCTTTGT‑C‑AACTCACCCGCCACCTGCA‑AATAAC‑GCGCCAATCATCACGCTCCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACTAGCAATAGTGGGCCGCGTAGcacaca                                                                                                                                                                                                  <  1:136931/249‑1 (MQ=255)
                                                           cTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAG‑CTTAATGAGAG‑CGAGCAAA‑GCGGTT‑CC‑GG‑TTATCCGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCACATC‑AAAATCACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGc                                                                                                                                                                                                >  1:223267/1‑251 (MQ=255)
                                                                  ataaaaaaaaaacagaaagaaCATTACCGCCCTAACGGTGTTGAATCTCAGGCGGGGCTTCGTTCACCCGAG‑TTTAAGGAGAG‑CGAGAAAA‑GCGTTT‑CC‑GG‑TTATCCGGG‑GGGCTTACCGGGGGTGTTGT‑C‑AAATCACCCGGCCCTTGCA‑AAAACC‑GCGCCAATCAGCGCGCCCCCGG‑AAATCCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGa                                                                                                                                                                                         <  1:66500/235‑1 (MQ=255)
                                                                  aGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAG‑CTTAATGAGAG‑CGAGCAAA‑GCGGTT‑CC‑GG‑TTATCCGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATg                                                                                                                                                                                          >  2:100332/1‑250 (MQ=255)
                                                                   gTAAAGATCAACCGGGAGAACCGTATCACCCTAACGGTGATGAATATCATGAAGCGCTTCGTTCACGCCAG‑CTTAATGAGAG‑CGAGCAAA‑GCGGTT‑CC‑GG‑TTATCCGCG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGAACCTGCG‑AATACC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCCCCACCAGCAATCGGCGGCCGCGTAGCACACAGCGGAATGa                                                                                                                                                                                         >  2:235955/1‑250 (MQ=255)
                                                                   gTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAG‑CTTAATGAGAG‑CGAGCAAA‑GCGGTT‑CC‑GG‑TTATCCGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCaccacc                                                                                                                                                                                                                            >  1:72224/1‑215 (MQ=255)
                                                                       aGAACAAACGGAAGAACAGAATCCCCCTAACGGTGATGAATCTAATGCGGCGATTCGTTCACGCCAG‑CTTAATGAGAG‑CGAGCAAA‑GCGGTT‑CC‑GG‑TTATCCGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCCCGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAAcc                                                                                                                                                                                     >  2:33616/1‑250 (MQ=255)
                                                                              aCGGAAGAGCAGTATCGCCAAAGCGGTGATGAACCGCATGCGGCGCTTCGTCCCCGCCGG‑CTTAATGAGAG‑CGAGCAAG‑GCGGTT‑CC‑GG‑TTACCCGGG‑CGGCGTACCGGGGGCGTTGT‑C‑AAATCAACCGGCCCCTG‑G‑AAAAACCGCTCAAACCAGCACGCCTCCGGAAAAAA‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTa                                                                                                                                                                             <  1:294883/251‑1 (MQ=255)
                                                                               cGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAG‑CTTAATGAGAG‑CGAGCAAA‑GCGGTT‑CC‑GG‑TTATCCGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTAc                                                                                                                                                                            >  2:115217/1‑251 (MQ=255)
                                                                                 gaaaaaacaacaccaccTAAGGGTAATGATTCTCATGGGGCGCTGC‑TTCACCCCAGAGTTAATGAGGG‑CGAGAAAA‑GGGGTT‑CC‑GT‑TTATCCGGG‑GGGCGACCCGGGGGCTTTCA‑C‑TAAACGCCCGCCCCCGAAG‑AAAACC‑GCCCCAATCACCACGCCACCGGAAAATA‑AAAATACCCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGa                                                                                                                                                                          <  2:255881/236‑1 (MQ=255)
                                                                                    aaaCAGTATCCCCCAAACGGTGATAACTCTCAGGCGGCGGTTCTTTCACCCCAG‑CTTAATGAGAG‑CGACCAAA‑GCGTTT‑CC‑GG‑TTACCCGGG‑GGGCGTACCGGGGGCGTTGT‑C‑AAATCAACCGCCCCCTGCG‑AAAAAC‑GCGCCAATCACCCCGCCTCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGt                                                                                                                                                                       <  1:112490/250‑1 (MQ=255)
                                                                                     aaCAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAG‑CTTAATGAGAG‑CGAGCAAA‑GCGGTT‑CC‑GG‑TTATCCGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTc                                                                                                                                                                      >  2:107901/1‑251 (MQ=255)
                                                                                     aaCAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAG‑CTTAATGAGAG‑CGAGCAAA‑GCGGTT‑CC‑GG‑TTATCCGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGAGGAATGAAACCAGTAACTACGAAGTc                                                                                                                                                                      >  1:90874/1‑251 (MQ=255)
                                                                                     aaCAGTACCGCCAAAAGGGTGATGAATCTCAGGCGGCGCTTCGTCCACGCCAG‑CTTAATGGGAG‑CGAGCAAG‑GCGGTT‑CC‑GT‑TTATCCGGG‑GGGCGTACCGGGGGCGTTGT‑C‑GAATCACCCGGCACCTGCA‑AATAAC‑GCGCAAACCAGCCCGCCTCCGGAAAATA‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTc                                                                                                                                                                      <  1:236767/251‑1 (MQ=255)
                                                                                         gtctcgccaaagcGGTGATGAAACTCAGGCGGCGTTTCGTTCACGCCAG‑CTTAAGGAGGG‑CGAGCAAG‑GGGGTT‑CC‑GT‑TAACCCGGG‑GGGCGT‑CCGGGGGGGTTGTTC‑GAATCAACCGGCACCTGGA‑AATAAC‑GCGCCAATCACCCCGCCCCCGGCAAATA‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATc                                                                                                                                                                  <  2:12297/239‑1 (MQ=255)
                                                                                         gtctCGCCCTAACGGGGATGAATCCCAGGCGGCGCTTCGTTCACGCCAG‑CTTAAGGAGAG‑CGAGCAAA‑GCGGTT‑CC‑GG‑TTATCGGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGAAAATA‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATc                                                                                                                                                                  <  2:107389/248‑1 (MQ=255)
                                                                                             cccccTAACGGTGATGAATCCCAGGCGGCTCTTCGTTCCCGCCAC‑CTTAA‑GGGAGGCGAGCAAC‑GCGGTT‑CC‑G‑‑TTTTCCGGGGCGGCGTCCCGGGGTCTTTGC‑C‑GAATCACCCGGCTCCTGCA‑AATAAG‑GCGCCAACCACCCCGCCCCCGGTAAAAC‑AAAAGAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGTGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGc                                                                                                                                                                <  1:24011/247‑1 (MQ=255)
                                                                                                ccTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAG‑CTTAATGAGAG‑CGAGCAAA‑GCGGTT‑CC‑GG‑TTATCCGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCAt                                                                                                                                                           >  1:19291/1‑251 (MQ=255)
                                                                                                  aaagggggagaattctaaggcggcgCTTTGTTCCCGCCAG‑CTTAATGAGAG‑CGAACAAG‑GCGGTT‑CC‑GG‑TTACCCGGG‑CGGCGTACCGGGGGCGTTGG‑C‑GAACCACCGGGCACCGGCA‑AAAACC‑GCGCCAATCAGCCCGCCTCCGGAAAATA‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTc                                                                                                                                                         <  2:322097/232‑1 (MQ=255)
                                                                                                    cgggggagaaatcccgtGCGCCGCTTCGTTCACCCCGG‑CGTAATGAGGG‑CG‑GCAAAAGCGGTT‑CC‑GT‑TTACCGGGG‑CGGCGTCCCGGGGGCGTTGT‑C‑GAAACAACCGGCCCCTGCG‑AATAAC‑CCGCCAACCAGCACGCCCCCGGCAAATC‑AAAAAAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGt                                                                                                                                                       <  2:141422/235‑1 (MQ=255)
                                                                                                                         cgcTTCGTTCCCCCCAG‑CTTAATGAGGG‑CAGGAAAA‑GGGGTT‑CC‑GG‑TTATCCGGG‑GGGCGTACCGGGGGCGTTAT‑C‑GAATCAACCGGCACCTACG‑AAAAAC‑TCGCCAATCAGCCCGCCTCCGGGAAATA‑AAAATAGCCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAGCTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGc                                                                                                                                  <  1:411580/251‑1 (MQ=255)
                                                                                                                              cGTTCACGCCAG‑CTTAATGAGAG‑CGAGCAAA‑GCGGTT‑CC‑GG‑TTATCCGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGAtgt                                                                                                                             >  2:411958/1‑251 (MQ=255)
                                                                                                                               gTTCACGCCAG‑CTTAATGAGAG‑CGAGCAAA‑GCGGTT‑AA‑GG‑CTATCCGCG‑CGGCGTACCGGGCGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAACACGCCTCCGGCAAATC‑ACAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAAACAGTCACTACGAAGTCGATCGCACCATTCGTCATCACAAAATGAACGTGGGCGAtgtg                                                                                                                            >  1:460878/1‑251 (MQ=255)
                                                                                                                                cgttccccccgtgttaaggagggggagcAAA‑GCGTTG‑CG‑GG‑TTACCCGGG‑CGGCGTACCGGGGGCTTTGT‑C‑AAACCAACCGACCCCGGCG‑AACACC‑GCCCCAATCAGCCCCCCCCGGGAAAATA‑AAAAAAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGAtgt                                                                                                                             <  2:94630/227‑1 (MQ=255)
                                                                                                                                   ccGCCAG‑CTTAATGAGAG‑CGAGCAAA‑GCGGTT‑CC‑GG‑TTACCCGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCCCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAAc                                                                                                                        <  1:361642/250‑1 (MQ=255)
                                                                                                                                     cccAG‑TTTAATGAGAG‑CGAGCAAG‑GCG‑TT‑CC‑GGGTTATCCGGG‑CGGCGTCCCGGGGGTGTTGC‑C‑GAATCACCCGGCCCCTGCG‑AATACC‑CCGCCAACCAGCCCGCCTCCGGAAAATA‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGt                                                                                                                      <  1:219653/250‑1 (MQ=255)
                                                                                                                                      ccAG‑CTAAAGGAGGG‑CGAGCAAA‑GGGGTC‑CC‑GG‑TTACCCGGG‑GGGCGCCCGGGGGGCGTT‑T‑CAGAACCACCGGGCACCGGCG‑AAAACC‑GCGCCAACCAGCACGCCTCCGGCAAATA‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTc                                                                                                                     <  1:279137/251‑1 (MQ=255)
                                                                                                                                       cAG‑CTTAATGAGAG‑CGAGCAAA‑GCGGTT‑CC‑GG‑TTATCCGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAGCGTCt                                                                                                                    >  2:98151/1‑251 (MQ=255)
                                                                                                                                        aG‑CTTAATGAGAG‑CGAGCAAA‑GCGGTT‑CC‑GG‑TTATCCGGG‑CGGCGTACCGGGGGAGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGACTGAAACCAGTAACTACGAAGACGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGtatg                                                                                                                   >  2:319922/1‑248 (MQ=255)
                                                                                                                                             tAATGAGAG‑CGAGCAAA‑GCGGTT‑CC‑GG‑TTATCCGGG‑CGGCGTCCCGGGGGCGTTGT‑C‑GAATCAAACGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AACATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATCGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTc                                                                                                                 >  1:200410/1‑249 (MQ=255)
                                                                                                                                                        gagcAAG‑GCGTTC‑CC‑GG‑TTATCCGGG‑CGGCTTCCCGGGGGCGTTGT‑C‑GAATCAACCGGCCCCGGCA‑AATAAC‑CCGCCAATCAGCACCCCCCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCgt                                                                                                     <  1:115217/251‑1 (MQ=255)
                                                                                                                                                                gCGGTT‑CC‑GG‑TTATCCGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAAt                                                                                              >  2:84299/1‑251 (MQ=255)
                                                                                                                                                                          gg‑TTATCCGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAAc                                                                                      >  1:143948/1‑251 (MQ=255)
                                                                                                                                                                              tATCCGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCACATGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCAATTC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTAGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCtt                                                                                   >  1:45929/1‑251 (MQ=255)
                                                                                                                                                                               aTCCGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTACGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTg                                                                                  >  1:391940/1‑251 (MQ=255)
                                                                                                                                                                                 ccGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GAGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGcc                                                                                >  1:381587/1‑251 (MQ=255)
                                                                                                                                                                                              ccGGGGGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAAcc                                                                    >  1:46221/1‑251 (MQ=255)
                                                                                                                                                                                                            c‑GAAACAACCGGCCCCGGCG‑AATAAC‑CCGCAAATCAGCACGCCTCCGCAAAATA‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGc                                                       <  1:417770/251‑1 (MQ=255)
                                                                                                                                                                                                                        gCACCTGCG‑AATAAC‑GCGCCAAACAGCCCGCCTCCGGCAAACC‑AAAATACCCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGAt                                            <  1:100332/251‑1 (MQ=255)
                                                                                                                                                                                                                         cACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATg                                           >  2:318778/1‑251 (MQ=255)
                                                                                                                                                                                                                            cTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGaa                                         >  1:94189/1‑250 (MQ=255)
                                                                                                                                                                                                                                          cccaaaTCAGCACGCCTCCGGAAAACC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGAt                            <  1:347181/247‑1 (MQ=255)
                                                                                                                                                                                                                                           gcCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATc                           <  1:348116/251‑1 (MQ=255)
                                                                                                                                                                                                                                               aTCAGCACGCCTCCGGCAAATC‑AAACTAACCGCCAGCAGAAGGCGAGTACCACCAGCAATAATGGGCCGCGTAACACACCGCGGAATAAAACCAGTAACTACGATGTCGATCGCACCATTCGTCAAACCAACATGAACGTGGGCGATGAGCACCGTCTGTCAGTCGCGGTCGCGGCGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGAc                       >  1:232846/1‑251 (MQ=255)
                                                                                                                                                                                                                                                             ggCAAATC‑AAAATAACCGCCAGCAGAAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACACAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCACATTGAAGATCTGCCCCGAGAGGCGATgg         >  2:334377/1‑251 (MQ=255)
                                                                                                                                                                                                                                                                aaaTC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAAt                                                                                                                                            >  1:148449/1‑117 (MQ=255)
                                                                                                                                                                                                                                                                    c‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTc  >  2:305692/1‑251 (MQ=255)
                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                           
ATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAG‑CTTAATGAGAG‑CGAGCAAA‑GCGGTT‑CC‑GG‑TTATCCGGG‑CGGCGTACCGGGGGCGTTGT‑C‑GAATCAACCGGCACCTGCG‑AATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATC‑AAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTC  >  NC_000913/2013973‑2014472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑