Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,278,657	T→C	V60A (GTT→GCT)	ghxP →	guanine/hypoxanthine transporter GhxP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,278,657	0	T	C	100.0%	204.1 / NA	62	V60A (GTT→GCT)	ghxP	guanine/hypoxanthine transporter GhxP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/29);  total (33/29)

GTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTTCACAACGTGGAAGCA‑CTGT‑CCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAACCTGTCTGGTTGCCGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGT  >  NC_000913/4278411‑4278900                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    gTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGATTACTCGGCGCCTGGGTTAAAAATTTACAAAGAGGGAGCA‑CCGT‑ACCTCAGGAAGTGGGTGCCGGGGTTACAAAGTTTCTTGCGATGGGCTACTACGTCATCGTCGTTTCCGGGATGTTGGGTAAAGCCGGCTTTCCGCCTGCGGCAGCTTTc                                                                                                                                                                                                                                                 >  1:299667/1‑251 (MQ=255)     cGCTGGCAGTGGATTGTCCGCCACGCATCTTAACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTTCACAACGTGGAAGCC‑CTGT‑CCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTg                                                                                                                                                                                                                                             <  2:325809/251‑1 (MQ=255)           cAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTTCACAACGTGGAAGCA‑CTGT‑CCGTCAGGAAGTGGGTGCCGGGTTAACAACGTTTCTGGGGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCt                                                                                                                                                                                                                                       >  1:507188/1‑251 (MQ=255)             ggggATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTTCACAACGTGGAAGCA‑CTGT‑CCGTCAGGAAGGGGTTGCCGGGTTAAAAAAGTTTCTGGCGGTGGTCTACTCGGGCATCGTCGGTCCCGGGATGTTGGGTAAAGCAGGGTTCCCGCCCGCGGCAGCTTTCGTTGCAAccgtt                                                                                                                                                                                                                                     >  2:234833/1‑248 (MQ=255)                 aTTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTTCACAACGTGGAAGCA‑CTGT‑CCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTgg                                                                                                                                                                                                                                 <  1:32419/251‑1 (MQ=255)                          cACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTTCACAACGTGGAAGCA‑CTGT‑CCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTAGGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGAc                                                                                                                                                                                                                        <  2:507188/251‑1 (MQ=255)                             gCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTTTACTCGCCGGCTGGTTTAAAATTTCACAACAGGGAAGCA‑CTGG‑CCGTCAAGAAGGGGTTGCCGGGGTAACAACGTTTCTGGCGATGGGGTACTCGGGCCTCGTCCGTCCAGGTATGTTGGGGAAAGCAGGCTTCCCCCCTGCGGCCGCATTCGTCTCAACCCGTCTGGtttgtggactcg                                                                                                                                                                                                                     >  1:359153/1‑241 (MQ=255)                                     aCGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTTCACAACGTGGAAGCA‑CTGT‑CCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATc                                                                                                                                                                                                             <  1:91114/251‑1 (MQ=255)                                              aaaCTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTTCACTGGACGCCTGGTTTAAAATTTCACAACGTGGAAGCA‑CTGT‑CCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGTTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGt                                                                                                                                                                                                    <  2:96593/251‑1 (MQ=255)                                               aaCTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTTCACTCGACCCCTGGTTTAAAATTTTCCAACGTGGAAGCA‑CTGTTCCG‑CCGGAAGAGGTTTCCGGGTTTACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAAGGATTTTGGGTAAAAGAGGCTTCCCGCCCGCCGCAGCTTTCGTTGGCACCCGTCTGGTTGGTGGGCTCGGTTCTATCggggtggggt                                                                                                                                                                                                   >  2:328131/1‑242 (MQ=255)                                               aaCTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGCTCACTCGTCGCCTGGTTTTAAATTTTACCACCTGGAAGCA‑CTTT‑CCGTCAGGGAGTGGGTGCCCGGGTAACAACGGTTCTGGCGATGGTCTACTCGGTCATCGTCGTTTCCGGGATGTTGGGTAAAGCAGGGTTCCCGCCCTCGGCAGCTTTCTTTGCAACCTGTCTTGTTGCGTGACTCGGTTCTATAGTGGTggggg                                                                                                                                                                                                   >  2:394957/1‑249 (MQ=255)                                                aCTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTTCACTCCACGCCTGGTTTAAAATTTCACAACGTGGGAGGA‑CTGT‑CCCTCAGGAAAAGGGTGCCGGGATAACAACGGTTTTGGGGATGGTCTACTCGGTCATCGTCGCTCCAGGGATGTTGGGTAAAACAGGCGTCCCGCCTGCGGCGGCTTTCGTTGCAACCTGTCTGGTGGCTGGACTCGGTTCTATCGTGAGGGGTCt                                                                                                                                                                                                  >  1:165138/1‑251 (MQ=255)                                                aCTCTCAGGGGATGTTTTCTTATGTCTACGCCAGCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTTCACAACGTGGAAGCA‑CTGT‑CCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGGGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTAGCGTGATGGGTCt                                                                                                                                                                                                  <  1:384535/251‑1 (MQ=255)                                                   ttcAGTGGATTTTTTTTTATTTCTACGCCATCAGCTGTTACGGGCGTTTCTCTCGACGCCGGTTTTAAAATTTCACACCGTGGAAGCA‑CTGT‑CCGCCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTCCTCGGTCATCGTCGTTCTAGGTATGTTGGGTAAAGCAGGTTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGTTTGCTGGACTCGGTTCTATCGTGATGGGTCtgt                                                                                                                                                                                                <  2:399333/249‑1 (MQ=255)                                                   ctcAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGGTTTAAATTTCACAAAGTGGAAGCA‑CTGT‑CCGTCAGGGAGAGGTTGCCGGGGTAACAACGTTTCTTGCGATGGTCTACTCGGGCATCGTCGTTTCAGGTATGTTGGGTAAAGCAGGGTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGGCGGACTCGGTTCTATCGTGTTGGGGCtggg                                                                                                                                                                                               >  1:176249/1‑249 (MQ=255)                                                        gggATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTTCACCACGTGGGAGCA‑CTGT‑CCGTCAGGAAGTGGGTGCCGGGTTAACAACGTTTCTTGCGCTGGTCTACTCGGTCATCGTCGTTCCAGGTATTTTTGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTTGTTGCAAACTGTCTGGTTTCTGGACCCGGGTCTCTCGTGATGGGTCTGTGGGCt                                                                                                                                                                                           >  1:5603/1‑250 (MQ=255)                                                               tttCTTATGTCTACGCCATCAGCGCGTACCGGCGGTTCACTCCACGCCCGGTTTAAAATTTCACAACGTGGAAGCA‑CTGT‑CCGTCAGGGAGTGGGTTCCCGGTTAACAACGTTTCTGGCGATGGTCTACTCGGGCATCGTCCTTCCAGGGATGTTGGGTAAAGCAGGCTTCCCCCCTGCGGCCGCGTTTGTTGCAAACTTTCTGGTTGCTGGGCTCGGTTCTATCGTGATggggtgttggggtaatatgcc                                                                                                                                                                                   >  2:18219/1‑233 (MQ=255)                                                                     aTGTCTACGCCATCAGCGCGTACCGGCGGTTCAATCGACGCCTGGTTTAAAATTTCACAACGTGGAAGCA‑CTGT‑CCGTCAGGAAGTGGTTGCCGGGGTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCCGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGGCTCGGTTCTATCGTGGTGGGTGTGTGGGGTAATATGCCGTTggg                                                                                                                                                                             >  1:384641/1‑250 (MQ=255)                                                                        tCTACGCCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTTAAAATTCACAACCTGGAAGCA‑CCGT‑CCGTCAGGAAGGGGTTGCCGGGTTTAAAAAGTTTTTGGCGATGGTCTACTCGGGCATCGGCGTTCCCGGGAAGTTTAGGAAAGCAGGGTTCCCGCCTGCGGCCGCTTTCGTTGGAAACTGTCTGGTTGCTTGACTCGGTTTTATCGTGGTGGGGCTTTggggtcatcttgcgttgggggt                                                                                                                                                                          >  2:396908/1‑232 (MQ=255)                                                                                     cgcgTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTTCACAACGTGGAAGCA‑CTGT‑CCGTCAGGAAGTGGGTGCCGGGGTAACAACGTTTCTGGCGATGGTCTACTCGGGCATCGTCGTTCCCGGGATGTTGGGGAAAACAGGCTTCCCGCCTGCGGCAGCTTTTGTTGCAACCTGTCTGGTTGCTGGACTCGGGTCTATCGTGATGGGGCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCAtc                                                                                                                                                             >  1:350150/1‑251 (MQ=255)                                                                                                 gTTCACTCGACGCCTGGTTTAAAATTTCACAACGTGGAAGCA‑CTGT‑CCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCg                                                                                                                                                 >  1:6170/1‑251 (MQ=255)                                                                                                   tCACTCCACGCCTGGTTTAAAATTGCACAACGTGGAA‑CATCTTT‑CCCTCAGGAATTGGTTGCGTGGTTAACAACGTTTCTGGCGATGGTCTACGCGGACATCCTTGTCCCAGGTATGGTGGGTAAAGCAGCCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTTGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCGGACAGCGt                                                                                                                                                <  2:299667/250‑1 (MQ=255)                                                                                                    cACTCGACGCCTGGTTTAAAATTTCACAACGTGGAAGCA‑CTGT‑CCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGttt                                                                                                                                              <  1:427209/251‑1 (MQ=255)                                                                                                           cGCCTGGTTTAAAATTTCACAACGTGGAAGCA‑CTGT‑CCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCAt                                                                                                                                       >  1:211770/1‑251 (MQ=255)                                                                                                           cGCCTGGTTTAAAATTTCACAACGTGGAAGCA‑CTGT‑CCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCAt                                                                                                                                       >  2:427235/1‑251 (MQ=255)                                                                                                             ccTGGTTTACAATTTCACAACGTGGAAGCA‑CTGT‑CCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGCCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCATGACCGCGTTTACCGCATTc                                                                                                                                     >  2:155174/1‑251 (MQ=255)                                                                                                                          ttCACAACGTGGAAGCA‑CTGT‑CCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTCTGTTGGGCAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCAGTTGGCGATTGGTTGCGCCATCACCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTgg                                                                                                                        >  2:211444/1‑251 (MQ=255)                                                                                                                          ttCACAACGTGGAAGCA‑CTGT‑CCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTTAGCCTGGTGCTg                                                                                                                         <  1:394387/250‑1 (MQ=255)                                                                                                                           tCACAACGTGGAAGCA‑CTTT‑CCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGAGATGGTCTTCTCGGTCATCGTCGTTCCAGTTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTc                                                                                                                                                                                                   <  1:301913/175‑1 (MQ=255)                                                                                                                           tCACAACGTGGAAGCA‑CTGT‑CCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGCTGGGTc                                                                                                                                                                                                   >  2:301913/1‑175 (MQ=255)                                                                                                                              caACGTGGAGGCA‑CTGT‑CCGTCAGGAAGTGTTTGCCGGTTAAACAACGTTTCTGGCTATGGTCTACTCGGTCATCGTCGTTCCAGTTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCa                                                                                                                    <  2:136838/251‑1 (MQ=255)                                                                                                                              caACGTGGAAGCA‑CTGT‑CCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGcc                                                                                                                    >  2:428619/1‑250 (MQ=255)                                                                                                                                     gAAGCA‑CTGT‑CCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATAtt                                                                                                             <  1:427235/251‑1 (MQ=255)                                                                                                                                          a‑CTGT‑CCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGt                                                                                                        >  1:289992/1‑251 (MQ=255)                                                                                                                                              gT‑CCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTAcc                                                                                                     >  1:224617/1‑251 (MQ=255)                                                                                                                                                       ggAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCgcgc                                                                                             <  1:486675/251‑1 (MQ=255)                                                                                                                                                          aGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTATCTGTCGCGCTgg                                                                                          <  1:394957/251‑1 (MQ=255)                                                                                                                                                                 gCCGGGTTAACAACGTTTCTGGGGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGc                                                                                                                                  <  1:25993/204‑1 (MQ=255)                                                                                                                                                                 gCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGc                                                                                                                                  >  2:25993/1‑204 (MQ=255)                                                                                                                                                                    gggTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGtt                                                                                <  1:234833/251‑1 (MQ=255)                                                                                                                                                                            aacGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTc                                                                               >  2:521327/1‑244 (MQ=255)                                                                                                                                                                            aacGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTc                                                                               <  1:521327/244‑1 (MQ=255)                                                                                                                                                                            aacGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATgg                                                                        >  1:15713/1‑251 (MQ=255)                                                                                                                                                                                tttCTGGGGATGTTCTACTCGGTCATCTTCGTTCCAGGTATGTGGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGgtg                                                                     <  2:328340/250‑1 (MQ=255)                                                                                                                                                                                     ggCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGt                                                               >  2:348798/1‑251 (MQ=255)                                                                                                                                                                                           ggTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACgg                                                         >  1:495762/1‑251 (MQ=255)                                                                                                                                                                                             tCTACTCGGTCATCGTCGTTCCAGGTAGGTTGGGTAAAGCAGGCTGCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGt                                                        <  2:289992/250‑1 (MQ=255)                                                                                                                                                                                                        aTCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCAc                                            <  2:350150/251‑1 (MQ=255)                                                                                                                                                                                                           gtcgtTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACggg                                         >  2:365169/1‑251 (MQ=255)                                                                                                                                                                                                              gtTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTAt                                      >  2:283317/1‑251 (MQ=255)                                                                                                                                                                                                                 ccAGGTAGTTTGGGTAAAGCAGGTTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATcc                                    <  1:59005/250‑1 (MQ=255)                                                                                                                                                                                                                                 aaGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTgcgc                   >  1:388300/1‑251 (MQ=255)                                                                                                                                                                                                                                    cAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAAc                <  1:348798/251‑1 (MQ=255)                                                                                                                                                                                                                                         ttCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGcc           >  2:206717/1‑251 (MQ=255)                                                                                                                                                                                                                                          tCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGcc           <  1:18219/250‑1 (MQ=255)                                                                                                                                                                                                                                          ccccGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCt          <  2:264592/250‑1 (MQ=255)                                                                                                                                                                                                                                           ccggCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTGGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTc         <  2:384641/248‑1 (MQ=255)                                                                                                                                                                                                                                           cccGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCt          <  1:110595/250‑1 (MQ=255)                                                                                                                                                                                                                                           cccGCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTc         >  2:427662/1‑251 (MQ=255)                                                                                                                                                                                                                                              gCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCt                                                                                                    >  2:400444/1‑157 (MQ=255)                                                                                                                                                                                                                                              gCCTGCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCt                                                                                                    <  1:400444/157‑1 (MQ=255)                                                                                                                                                                                                                                                  gCGGCAGCTTTCGTTGCAACCTGTCTGGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGgtgt  <  1:310508/251‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    GTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTTCACAACGTGGAAGCA‑CTGT‑CCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAACCTGTCTGGTTGCCGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGT  >  NC_000913/4278411‑4278900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑