Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,123,930	A→T	V176E (GTA→GAA)	cytR ←	DNA‑binding transcriptional repressor CytR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,123,930	0	A	T	100.0%	261.3 / NA	66	V176E (GTA→GAA)	cytR	DNA‑binding transcriptional repressor CytR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (26/40);  total (26/40)

AGCGCCATCACATCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTACTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTG  >  NC_000913/4123684‑4124176                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        aGCGCCATCACATCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGc                                                                                                                                                                                                                                                     <  1:167519/251‑1 (MQ=255)          acaTCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGCCGCAGCGCCTGAACATAGCCTTGCAGGCGGTTGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATgc                                                                                                                                                                                                                                            <  2:335793/251‑1 (MQ=255)                cTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCCTCTCTTCGGGACCGGCTATACAGCCAAATCGTTTATGCCCTTGCTCATATAAATAATTTTCTTCATCAAATTCGGCGGt                                                                                                                                                                                                                                      >  2:48232/1‑251 (MQ=255)                  aTGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTc                                                                                                                                                                                                                                     >  1:106232/1‑250 (MQ=255)                      cAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGAtt                                                                                                                                                                                                                                <  2:72538/251‑1 (MQ=255)                                        ggTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCCGATTGTCGATATGAACTGTAgg                                                                                                                                                                                                              >  1:296558/1‑251 (MQ=255)                                            gtgGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGGTCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCGGGCGGAAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGGTTATGCCCTTGCTCATCTAAATAATTTTCTGCATCAAATGCGGCGGGTAGATTGTTGGAATGAACTCTAGGcaac                                                                                                                                                                                                          >  1:66481/1‑249 (MQ=255)                                            gtgGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGcag                                                                                                                                                                                                           <  2:490525/250‑1 (MQ=255)                                             tgGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAAATGTAGGcagcc                                                                                                                                                                                                         >  1:433962/1‑250 (MQ=255)                                              ggAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGcagctc                                                                                                                                                                                                        <  2:296558/251‑1 (MQ=255)                                                     cAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGcagctccagctc                                                                                                                                                                                                  <  1:35167/250‑1 (MQ=255)                                                        gcagcTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGt                                                                                                                                                                                              <  2:65005/251‑1 (MQ=255)                                                               ctgcATCGCTTTACTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGAGTGTCGATATGAACTGTAGGCAACTCCAGCTCCGGGGCAAAtt                                                                                                                                                                                       >  1:381720/1‑251 (MQ=255)                                                                tgcATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTTGATATGAACTGTAGGCAGCCCCAGCTCCGGGGCAAAttt                                                                                                                                                                                      >  1:432281/1‑250 (MQ=255)                                                                  cATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGGCAGATTTTCGATATTAAATGTAGGgagctccagccaccgtgcaaaattgt                                                                                                                                                                                    >  2:261385/1‑235 (MQ=255)                                                                   aTCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAAttcgtt                                                                                                                                                                                   <  1:142081/251‑1 (MQ=255)                                                                   aTCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGGGACACAGCGGCATCTCTTCGGGACCGGCTATTCAGCCAATCCGTTTATGCCCTTTCTCATATAAATAATTTTCTGCATCAAATGCGGCGGGCCGATTGTCGATAAGAACTTTTGGCAACTCCAGCTCCGGTGCaaaattggt                                                                                                                                                                                   >  2:66597/1‑245 (MQ=255)                                                                     cGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAAttcgttc                                                                                                                                                                                  <  1:325443/250‑1 (MQ=255)                                                                              tCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCAc                                                                                                                                                                          <  2:118979/249‑1 (MQ=255)                                                                                cGGCTTCGAAGGGGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATc                                                                                                                                                                      <  2:73702/251‑1 (MQ=255)                                                                                 ggCTTCGAAGGTGAAGTCCTCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTTCCGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTGTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATcg                                                                                                                                                                     <  2:375689/251‑1 (MQ=255)                                                                                         aGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGACGCAGCGACGCAGCGCCTGAACATAGCCTTGAAGGCGGTAGTGACACAGCGGCATCTATTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATTAAAt                                                                                                                                                                                                                                              >  1:529161/1‑170 (MQ=255)                                                                                         aGGTGAAGGCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAAt                                                                                                                                                                                                                                              <  2:529161/170‑1 (MQ=255)                                                                                             gAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑Ac                                                                                                                                                        <  1:214402/251‑1 (MQ=255)                                                                                                gTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCt                                                                                                                                                     <  2:500830/251‑1 (MQ=255)                                                                                                   gCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGtt                                                                                                                                                  <  2:263481/251‑1 (MQ=255)                                                                                                    ccACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTc                                                                                                                                                 <  1:297862/251‑1 (MQ=255)                                                                                                         ggCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCt                                                                                                                                                     <  1:3508/242‑1 (MQ=255)                                                                                                             aTGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCt                                                                                                                                        <  2:500417/251‑1 (MQ=255)                                                                                                               gTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCCCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCCGTTCCTCAATGCTgg                                                                                                                                      >  1:34338/1‑251 (MQ=255)                                                                                                                  ttGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGAAGTGACACAGCGGCATCTCTTTGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGGCCGATTGTCGCTATTAACAGTAGGCAGCTCCAACTCCGGTGCAAATTCGATCGCCATCACCATCGGCGGCAGGTT‑ACGCTGTTCCTCAATgattgcct                                                                                                                                   >  2:111137/1‑244 (MQ=255)                                                                                                                        aTCAACCATAATGCCGCAGCGACGCCGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGGCAGATTGTCGATATGAACTGTAGGCCGCTCCAGCTCCGGGGGAAATTCGGTCGCCATCACCCTCGGCGGCAGATT‑ACGCTGTTTCTCAATGCTGGcctaaaa                                                                                                                               >  2:143348/1‑243 (MQ=255)                                                                                                                                 aaTGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTTTCGATATGAAATGGAGGGAGCCCCCGCTCCGGGGCCAATTTGGTCCCCATCCCCATCGGCGGCAGATTTAGCCTGTTC‑TCCATTCTGGCCTCAAACCGCAGccctt                                                                                                                    >  2:101790/1‑248 (MQ=255)                                                                                                                                 aaTGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCtt                                                                                                                     <  2:106232/250‑1 (MQ=255)                                                                                                                                      cGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAAccc                                                                                                               <  1:549012/251‑1 (MQ=255)                                                                                                                                      cGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAAccc                                                                                                               <  2:432281/251‑1 (MQ=255)                                                                                                                                            gACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCCCCGGGGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCCATTCTGGCATCAAACGGCAGCCCTTAACCCAGccac                                                                                                         >  1:91629/1‑248 (MQ=255)                                                                                                                                                   cgcCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGc                                                                                                  <  2:34338/251‑1 (MQ=255)                                                                                                                                                    gcCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATg                                                                                                   <  1:24963/249‑1 (MQ=255)                                                                                                                                                    gcCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGcc                                                                                                 <  2:201442/251‑1 (MQ=255)                                                                                                                                                       tGAACATAGCCTTGCAGGCGGTAGGGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCCGATTTTCGCTATGAACTGTTGGCCGCTCCCGCTCCCGGGCCAATTTGGTTGCCATCCCCCCCGGCGGCAGATT‑TTCCCGTTCCCCAATTCTGGCCTCAAAACGCCGCCTTGTACCCACCAACAACCTGccctc                                                                                              >  2:429428/1‑248 (MQ=255)                                                                                                                                                        gAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATc                                                                                              <  1:51219/250‑1 (MQ=255)                                                                                                                                                         aaCATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCaa                                                                                            <  1:182870/251‑1 (MQ=255)                                                                                                                                                                         gggTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTgatgat                                                                            <  2:436215/250‑1 (MQ=255)                                                                                                                                                                         cGGTAGTGACACGGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACCGTAGGCAGCTCCAGCTCCGGGGCAAATTCGTTCGCCATCACCATCGGCGGGAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCCTGAACCCAGCAACAACATGCCATCAATTTGCTTGGtgattat                                                                            >  1:382311/1‑248 (MQ=255)                                                                                                                                                                         cGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTgatgat                                                                            <  1:470514/251‑1 (MQ=255)                                                                                                                                                                              gTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAAt                                                                       <  2:191662/251‑1 (MQ=255)                                                                                                                                                                                gACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCg                                                                     <  2:232922/251‑1 (MQ=255)                                                                                                                                                                                      gCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTTAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGGTaaa                                                                >  2:8525/1‑250 (MQ=255)                                                                                                                                                                                      gCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAg                                                               <  1:15948/251‑1 (MQ=255)                                                                                                                                                                                       cGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGATCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAgg                                                              >  1:289295/1‑251 (MQ=255)                                                                                                                                                                                        ggCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCCTGAAACCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAggg                                                             >  2:402567/1‑250 (MQ=255)                                                                                                                                                                                             ctctTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGtttttt                                                        >  1:316047/1‑251 (MQ=255)                                                                                                                                                                                               ctTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGGTCGCCATCACCATCGGCGGCAGATT‑ACGCTGGTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGttttt                                                         <  1:241876/248‑1 (MQ=255)                                                                                                                                                                                                 tCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGGGATGATCAAATCGATAAAGGTTTTTTCctg                                                    >  2:404044/1‑251 (MQ=255)                                                                                                                                                                                                 tCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTGTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTcc                                                      <  2:187204/249‑1 (MQ=255)                                                                                                                                                                                                      aCCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGAt                                               >  1:110164/1‑251 (MQ=255)                                                                                                                                                                                                          gCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTc                                                       <  1:155990/239‑1 (MQ=255)                                                                                                                                                                                                          gCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAAACCAGCAAAAACATGCCATCAATTTGCTTGGGGATGATCAAATCGATAAAGGTTTTTTc                                                       >  2:155990/1‑239 (MQ=255)                                                                                                                                                                                                               aCAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATgc                                       <  1:95388/250‑1 (MQ=255)                                                                                                                                                                                                                  gcctattcGTTTATGCCCTTGCTTATGTCAATAATGTTCTGCTTCAGATGCGGCGGTCAGATTGTCGAGATGAACTGTAGGCAGCACCAGCGCCGGTGCAAACTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTTGCATCAAACGGCAGCCGTGAACCCAGCATCAACATGCCATCATTTTGCTTGGTGAAGATCAAATCGCTAAAGGTTTTTGCCTGCTGATTTTGATGCGcaa                                   <  2:224990/244‑2 (MQ=255)                                                                                                                                                                                                                             tATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAAt                         <  2:433962/250‑1 (MQ=255)                                                                                                                                                                                                                                 cccTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGc                     >  2:28153/1‑250 (MQ=255)                                                                                                                                                                                                                                         cATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGata             <  1:462811/250‑1 (MQ=255)                                                                                                                                                                                                                                          atatAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATc           <  1:554017/251‑1 (MQ=255)                                                                                                                                                                                                                                                   atTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTg  >  1:90262/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        AGCGCCATCACATCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTACTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATT‑ACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTG  >  NC_000913/4123684‑4124176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑