| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,782,919 | 0 | T | A | 28.0% | 69.1 / 10.1 | 53 | D248V (GAT→GTT) | gpsA | glycerol‑3‑phosphate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (18/18); new base A (13/1); total (32/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.95e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.70e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCC‑ACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTG > NC_000913/3782679‑3783157 | aGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGcc < 1:137762/250‑1 (MQ=255) tCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGGCTGGTTGTCGGTAAAGGGAAGCACCAGAACGCCAAGCCCCGCCATg > 1:267665/1‑251 (MQ=255) aTGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGccc < 1:258933/251‑1 (MQ=255) cggcctgccgcgggTTTTTCCCGAAATATAATACTTAATAATTTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCGGTCCGGGGCTTTCTTTCGTATTGCGGTAGCCTTCCCCCCCCGGCCCAATCTTCCCCTGCGCGCTTTGTACATCCATGCCCTCACCGAGCATCAGGCCAAAACGGCGGTTACGCGACTGGTTGTGGTTAAAGGTAATCCCAATAACGCAAAGCCCCGCCATGCCCA‑Ta < 2:391580/243‑1 (MQ=255) cTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGCCTGGTTGTCGGGACAGGGGAGCACCAGAACGCCAAACCCCGcccttcccctaaa > 2:46319/1‑238 (MQ=255) tttCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTAAGCGACTGGTTGTCGGGACAGGTGAGCACCAGATCGCCAAACCCCGCCCTGCCCA‑TAAAGGTGGAAGGGTCGGc > 1:218280/1‑251 (MQ=255) gCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGCCCGGGTGTCGGTTAAGGGAAGCACCACATCGCCCAACCCCCCCATGCCCA‑TCAAggggggcggggcggccaccc > 2:432863/1‑233 (MQ=255) aaTATAATACTTGATAAATTTCCTCGGTTATTGGAATTTCACCGCCGAAGCGAGGCGCCAGTTGGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCCCCTGCGCGTTTTGTACATCCAGGCCCGGACCGAGCATCATGCCAAAACGGCGTTTACGCGATTGTTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAgc < 2:211370/251‑1 (MQ=255) taataCTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACCCGACTGGTTGTCGGTACAGGTAAGCACCCGATCGCCAAGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGcgccg > 1:332330/1‑251 (MQ=255) ataCTTGATAAATTTCCTGGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCCCCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGCCTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTc < 2:316387/235‑1 (MQ=255) ataCTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGGTACGCGACTGGTTGTCGGTTCAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCA‑TAAAGGGGGCAGGGTc > 1:316387/1‑235 (MQ=255) tGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGGTTTCGGTACAGGGAAGCACCAGATCGCCCAGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCCCCaa > 1:11240/1‑250 (MQ=255) ccTCGTTTATTGCCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCTTATTGCGGTAGCCTTCCCCCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTCCGCGACTGGTTGGCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACAt < 1:101452/250‑1 (MQ=255) gTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGGACAGGGAAGCACCAGATCGCCAAGCCCCGCCATGCCCA‑TAAAGGTGGGAGGGTCGGGCCCCAGCGCCGCACCAAGAAGCGACATTTcagc > 1:453232/1‑251 (MQ=255) ggCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTCCCACCACCTGACCAATCTTCTCCTGCGCGCTTTTTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGGGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGccc < 1:488543/251‑1 (MQ=255) aTTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTAAGCGACTGGGTGTCGGTACAAGTAAGCACCAAACCGCCAAACCCCGCCATTCCCC‑TTAAAGTGGCAGGGTCCGCACCCCACCCCGCACCAAGACGCCACATTTctgcccacccacc > 2:109483/1‑243 (MQ=255) aaCGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACACCACCGGTTTTCGGCACAGGGAAGCACCAAAACGCCCAGCCCCGCCATTCCCA‑TAAAGGGGGCAGGGTCGGCACCCCCCGCCCCCCCAAAACGCGCCACTTCAGCCCGCCCCACgggg > 1:483907/1‑249 (MQ=255) aCGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTAAGCGACTCGTTGTCGGTACACGGAAGCACCAAAACGCCAAACCCCGCCATGCCCC‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCCCCAAGAAAGGACATTTCAGCCACCCC‑ACGGGTGAt > 1:336130/1‑251 (MQ=255) gCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGGAAGCACCAGATCGCCAAACCCCCCCCTGCCCA‑TAAAGGGGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCATCCAGCCC‑CCGGGGGATc > 1:490007/1‑250 (MQ=255) ggctaTGCGCCAGTTCGCGGCCTTCTTTCGTATTGCGGTACCTTTCCACCCCCTGACCAATCTTCTCCTGCCCGCTTTGTACATCCATGCCCTGACCGAGCATCATCCCAAAACGGGGTTTACGCGGCTGGTTTTCGGTACAGGTACGCACCAGATCGCCAAGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCC‑ACGGGTGATCAGCGcc < 1:307427/247‑1 (MQ=255) cGATGCGCCAGTTCGCGTACTTCTTTCGTATTGCGTTAGCTTTCCACCCCCTGACCAATCTTCCCCGGCCCTTTTTGTACATCCATGCCCTGACCGAACATCAGCCCAAAACGGCGTTTACGCGACTGGTTGTCGGTACAGGTAAGCCCCAGATCGCCAAGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCC‑ACGGGTGATCAGCGCCGt < 1:282960/251‑1 (MQ=255) ccAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACCGGGTTTCGGTACAGGTAAGCACCAAATCGCCCAGCCCCCCCATGCCCACAAAAGGGGGC‑GGGTGGGCCCCCCGCGCCCCCCCCCGACGCGGCAATTCAGCCAGCCC‑ACGGGGGAGCCGCGCCCTACgcgcc > 2:143311/1‑250 (MQ=255) aGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTAAGCGACTGGTTGTCGGGACAGGGAAGAACCAGAACGCCAAACCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGGACCCAGCGCCGCACCCAAAAGCGACATTTCAGCCAGCCC‑ACGGGGGATCAGCGCCGTACgcgcctt > 1:440596/1‑248 (MQ=255) gTTCGCGGCCTTCTTTCGTATTGCGGTAGCCTCCCCCCCCCTGACCAACCTTCTCCGGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTCCGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCC‑ACGGGTGATCAGCGCCGTACGCGCATTc < 2:490007/251‑1 (MQ=255) tCGCGGACTTCTTTCGTATTGCGTTAGCCTTCCACCCCCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGGGCATCATGCCAAAACGGCGGTTACGCGACTGGTTTTCGGTACAGGTAAGCCCCAGATCGCCAAGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCC‑ACGGGTGATCAGCGCCGTACGCGCATTCg < 1:387869/250‑1 (MQ=255) cgGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGCCTGGGTTTCGGTGCAGGGAAGCACCAGAACGCCAAGCCCCCCCACGCCCA‑TAAAGGTGGCAGGGGCGGGACCCCGCGCCGCACCCAGCCGCGGAATTTCAGCCAGCCC‑ACCGGTGATCAGCGCCGTCCGCGCACTCGcccc > 2:18568/1‑248 (MQ=255) ttCGTATTGCGGTAGCCTTCCACCCCCTGCCCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGGGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCC‑ACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAAcc < 1:109483/248‑1 (MQ=255) ttCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACCCGGCTGGTTTTCGGGACAGGGAAGCACCAGAACGCCAAGCCCCGCCATGCCCA‑TAAAAGTGGCAGGGGCGGGACCCCGCGCCCCACCAAAACGCGGCATTTCAGCCAGACC‑CCGGGGGATCAGCGCCGTACGCGCATTCGCACCACAAccca > 1:362847/1‑248 (MQ=255) aTTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGCTACGCGACTGGTTTTCGGTACAGGGAAGCACCAGAACCCCAAGCCCCGCCCTGCCCC‑TAAAGGGGGCAGGGGCGGCACCCCGCGCCGCACCAAGACGCGACATTTCAGCCCACCC‑ACGGGGGATCAGCGCCGTACGCGCATTTGCACCAAAACCGATACCGt > 1:285985/1‑251 (MQ=255) cGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACACGACTGGTTGTCGGTACAGGGAAGCAACAGAACCCCAAGCCCCCCCCTGCCCC‑TAAAAGGGGGAGGGGCGGCACCCAGCGCCGCACCCAGACCCGACATTTCCGCCCGCCC‑CCGGGGGATCAGCGCCGCACGCGCCTTCGCACCAAAAACGATACCGTCggg > 1:90675/1‑250 (MQ=255) gCCTCCCACCACCGGACCACTCTTCTCTTGCGCGCTTTGTACCTCCATGCCCGGACCGGGCATCATGCAAAAACGGCGGTCACGCGACTGTTGGTGGGTACAGGAAAGCACCAGATCGCCAAGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCC‑ACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATc < 2:218280/250‑1 (MQ=255) ccttcccacccCCTGACCAATCTTCTCCGGCGCGCTTTGTACATCCATCCCCTGACCGAGCATCATGCCAAACCGGCGGTTACGCGACTGGTGGTCGGTACAGGTAAGCACCAGATCGCCAACCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCC‑ACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATcc < 2:453232/246‑1 (MQ=255) accaccTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGAACGCCAAGCCCCGCCATGCCCA‑TAAAGGGGGCAGGGTCGGCACCCAGCGCCCCACCAAGACGCGGCCTTTCAGCCAACCC‑CCGGGGGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGcccc > 1:216148/1‑248 (MQ=255) tcCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGGTGTCGGTACAGGTAAGCACCAGAACGCCAAGCCCCCCCCTGCCCA‑TAAAGGTGGCAGGGGCGGCACCCAGCGCCGCACCAAAACGCGCCATTTTAGCCAGCCC‑ACGGGTGATCAGCGCCGTACGCGCATTCTCACCACAACCCATACCGTCGGACATCCCCGCACCAATGTCAAAAACGttttt > 1:362250/1‑251 (MQ=255) ccgctctttttcaacccATGCCCTGACCGACCATCATGCCAAAACGGCGGTTAGGCGACTGGTTGTCGGTACATGTAACCCCCAGATCGCCACGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCC‑ACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCAcc < 2:328743/236‑1 (MQ=255) ngcgcTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACCCGACTGGTTTTCGGCACAGGTAAGCACCAGAACCCCAAACCCCCCCATGCCCA‑CAAAAGTGGCAGGGGCGGCACCCCGCCCCCCACCCAAACGCGACATTTCCGCCCCCCC‑ACGGGTGAACAGCGCCGCCCGCGCACTCCCCCCCAAACCGATACCCGCCGACATCCCCCCACCCATGGCCACAACCGTTTTCAccc > 2:245722/2‑250 (MQ=255) gTACATCCAGGCCCTGACCGAGCATCATGCCAAACGGGCGGTTACGCGACTGGTTGCCGGTACAGGTAAGCACCAGATCCCCAAGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCC‑ACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCgccgcc < 1:143311/250‑1 (MQ=255) cATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGAACGCCAAACCCCGCCATGCCCA‑TAAAGGGGGCAGGGGCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCC‑ACGGGGGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCgg > 1:255067/1‑200 (MQ=255) accaggcaaaaaCGGCGGTTCGGCGACAGTTTGCCGGTACAGGTAGGCACGAGACCGCCATGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCC‑ACGGGGGATCAGCGcc < 1:65511/142‑1 (MQ=255) ggcggTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCC‑ACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAAcg > 2:390594/1‑251 (MQ=255) ggTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGACGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCC‑ACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATCACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCgg > 2:134589/1‑251 (MQ=255) gTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTcagcc < 1:88440/112‑1 (MQ=255) gTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTcagcc > 2:88440/1‑112 (MQ=255) gTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCAATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCC‑ACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCgg > 1:294609/1‑250 (MQ=255) tACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCC‑ACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGCTTGCTGTAAACGCGGaa > 1:399709/1‑250 (MQ=255) cgcgACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCC‑ACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCCATGAAATCCGGATTGCTGTAAACGCGGaaaat > 2:438624/1‑249 (MQ=255) gACTGGTTGTCGGTACAGGTAAGCCCCAGATCGCCAAGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCC‑ACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCAc < 1:330717/212‑1 (MQ=255) gACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCC‑ACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCAc > 2:330717/1‑212 (MQ=255) ggTTGTCGGTCAAGGTAAGCACCAGACCGCCAAGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCC‑ACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGcc < 1:541416/250‑1 (MQ=255) gTAAGCACCAGATCGCCAAGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCC‑ACGGGTGATCAgc > 2:549590/1‑105 (MQ=255) gTAAGCACCAGATCGCCAAGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCC‑ACGGGTGATCAgc < 1:549590/105‑1 (MQ=255) gTAAGCACCAGATCGCCAAGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCC‑ACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAgctg > 2:504501/1‑251 (MQ=255) aGCACCAGATCGCCAAGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCC‑ACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGcc > 2:191557/1‑194 (MQ=255) | AGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCA‑TAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCC‑ACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTG > NC_000913/3782679‑3783157 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |