| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,550,663 | 0 | A | C | 33.3% | 153.4 / 13.4 | 75 | N68T (AAC→ACC) | fdnH | formate dehydrogenase N subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (42/8); new base C (0/25); total (42/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.64e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTTAGTCAACATCGAGAAGGCGTAAGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGC‑AACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAAC‑TTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCATTGCCGGGTGTCCGTTTAATATTCCG > NC_000913/1550424‑1550904 | cTTAGTCAACATCGAGAAGGCGTAAGGGGGCGAACAGATGGCTATGGCAACGCAGGACATTATCAAAAGATCCGC‑AACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAATCCCAAAAC‑TTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGATGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATc > 1:324472/1‑251 (MQ=255)cTTAGTCAACATCGAGAAGGCGTAAGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGC‑AACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAAC‑TTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATc > 2:326495/1‑251 (MQ=255) ttAGTCAACATCGAGAAGGCGTAAGGGGGCGAACAGATGGCTAGGAAAACGCAGAACATTATCAAAAGGTCCGA‑AACTAACCCCATCACGCCGCTTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAAC‑TTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCGGGTGGCGTGTTCGGAG‑TGGAACGACACCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATc < 2:185290/250‑1 (MQ=255) aGTCAACATCGAGAAGGCGTAAGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGC‑AACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAAC‑TTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTg > 1:247925/1‑250 (MQ=255) aaCATCGAGAAGGCGTAAGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGC‑AACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAAC‑TTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAgcgc > 2:193974/1‑251 (MQ=255) agaAGGCGTAAGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGC‑AACTACCTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGAAAAAC‑TTATCGACTTTTCCCCCTGTATCGGTTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGTTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTcc < 1:409492/251‑1 (MQ=255) gaAGGCGTAAGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAGGGTCCGA‑AACTAACCCCATCACGCCGCCTTCTCAGGTGCGTGATTCCAAAGCAGAAGTCGCAAAAC‑TTATCGACTTTTCCCCCTGTATGGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑GGGAACGCCATCCGGGATGAAGTGGGGCACTGCGTGGGGGTTT‑ACGATACCCCCGCCGATCTGAGCGCCAAGTCCt < 1:310174/251‑1 (MQ=255) gaAGGCGTAAGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAACGGTCCGA‑AACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAAC‑TTATCGACGATTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGGTTTAGGAG‑AGGAACGACCTCCGTGAAGAAGTGGGGCACTGCGTCGGGGGTT‑ACGATAACCCAGCCGCTCAGAGCGCCaatgcct > 2:82479/1‑246 (MQ=255) aaGGCGTAAGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGC‑AACTAACTCCATCACGCAGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAAC‑TTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAAACCCGCCAATCTGAGCGCCACGTCCTg > 1:34842/1‑251 (MQ=255) gCGTAAGGGGGCGAACAGATGGCTATGAAAACGCAGAACATTATCAAAAGGTCCGC‑AACTACCCCCATCCCGCCGCTTTCTCAGGTGCGTGATTAAAAAGCAGAAGTCGCAAAAC‑TTATCGCCGTTTCCCCCTGTATCGGCTGTAAGGCCT‑TTCAGGTGGCGTGTTCGGAG‑GGGAACGACACCCGTGATGAAGTGGGGCCCTGCGTCGGGGTTT‑ACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGAc < 1:258770/251‑1 (MQ=255) gaggggggCGACAAGAGGGCGATGGAAACGCAGTACATTATCAAA‑GGTCCCCCATCTAACCCCACCACGCCCCCTTCTCTGGGGCGTTATAACAAAGCAGAACTCGAAATAC‑GTTTCGCTGTTCCCCCTTGTCTGGGTTGTAA‑GCCTTGTCGGGGGGCGTGTTCGGGG‑TGGAACCCCACCTGTGATGAAGTGGGGCCCTG‑GTGGGGGTTTTGCTATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGt < 2:333401/248‑1 (MQ=255) aGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGC‑AACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAAC‑TTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTg > 1:216613/1‑250 (MQ=255) gccGAACAGGTGGCTGTGGAAACGCAGGAAATTATCAAAAGGCCCCC‑ACCCAACTCCATCCCGCCGCCTTCTCAGGTGCGTGTTTACAAGGAAGAAGTCAAAAACC‑TTATCGACGTTTCCCCCTTTATCGGCTGTAAGGCCT‑GTGGGGTGGGGGGTTGGGAG‑GGGAACGACATCCGTGATGAAGTGGGGCGCTGCGGGGGGGTTT‑ACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATgcg < 2:379553/249‑1 (MQ=255) cGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGC‑AACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAAC‑TTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCt > 1:488830/1‑251 (MQ=255) gAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGC‑AACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAAC‑TTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCtt > 1:533908/1‑251 (MQ=255) gAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGC‑AACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAAC‑TTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGGGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCtt > 2:507044/1‑251 (MQ=255) aGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGC‑AACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAAC‑TTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGc > 1:295584/1‑251 (MQ=255) aGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGC‑AACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAAC‑TTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGACGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGc > 2:297997/1‑251 (MQ=255) aggtcTATGGAACCGCAGGACATTATAAAAGGGTCCGC‑ACATAACTCCATCACGCCGCTTTCTCAGGTTCGTGATTAAAAAGCAGAGGTCGAAAAAT‑TTATGGCCGTTCCCGCCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTTTTGGGAG‑TGGACCGACACCCGTGATGAGGTGGGGCCGTGGGTGGGGGTTT‑CCAATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGa < 2:411861/247‑1 (MQ=255) gCTATGGAAACGCAGGACATAAACAAAAGGTCCGC‑AACTACCCCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGAAAAAC‑TTACCGCCGTTTCCACCTTTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATAAAGTGGGGCACTGCTTCGGGTTTT‑ACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAAc < 1:328068/251‑1 (MQ=255) tATGGAAACGCAGGACATTATCAAAAGGTCCGC‑AACAAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAAC‑TTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCg > 2:117270/1‑251 (MQ=255) agggAACCGCAGAACATTATCAAAAGGTCCGC‑AACTAACCCCACCACGCCGCCTTCTCAGGTGGGTGATAAAAAAGCAAAAGTCGAAAAAC‑TTATCGCCGTTCCCACCTGTATCGGCTGTAAAGCCT‑GTCGGGGGGGGTGTTCGGGG‑TGAAACGACACCCGTGATGAAGTGGGGCACTGCGTGGGGTTTT‑ACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGc < 2:413483/241‑1 (MQ=255) aTGGAAACGCAGGACATTATCAAAAGGTCCGC‑AACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAAC‑TTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGc > 1:413483/1‑243 (MQ=255) ggaaaccgcggaacataataaaagggTCCGC‑AACAAACCCCATCACGCCCCTTTCTGAGGTGTGTGATTACAAAGCAGAAGTCGCAAAAC‑TTATGGACGTTCCCCCCTGTATCGGTTGAAAAGCCT‑GGCAGGGGGCGTTTTCGGGG‑TGGAACGACACCCGTGATGAGGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATATGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGaa < 1:440524/227‑1 (MQ=255) cgcgggACATAATCAAAAGGTCCGC‑AACTAACCCCACCACGCCGCTTTCTCAGGTGCTTGATTACAAGGCAGAAGGC‑CAAAACCTTATCGTCGTTTCCACCTGTACCGGCTGTAAAGCCT‑GTCAGGGGGCGTGTTCGGAG‑TGGAACGACATCCGTGAGGAAGGGGGGCACTGCGTGGGGTTTT‑ACGAAACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAAc < 1:46078/247‑1 (MQ=255) gCAGGACATTAACAAAAGGTCCGA‑AACTAACCCCATCCCGCCGCTTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGAAAAAC‑TTATCGACGTTTCCCCCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACg < 1:193974/251‑1 (MQ=255) aaaGGTCCGC‑AACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAAC‑TTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTgg > 1:127554/1‑251 (MQ=255) tCCGC‑AACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAAC‑TTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGGGGCTggt > 2:291940/1‑249 (MQ=255) ccGC‑AACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTAAAAAGCAGAAGTCGAAAAAC‑TAATCGACGTTCCCACCTGTATCGGCTGAAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGGGGGGCACTGCGTCGGGTTTT‑ACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATc < 2:134881/251‑1 (MQ=255) aacccccATCACGCCGCCTTCGCAGGGGTTTGTTTACAAAGCAGAAGTCGAAAACC‑TTATCGCGGTTTCCACCTGTAGCGGCTGTAAAGCCT‑GCCAGGTGGCGTGTGCGGGT‑TGGAACGACTGCCGTGATGAAGGGGGGCCCTGCGCCGGGGTTG‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAg < 2:174304/246‑1 (MQ=255) accccaacccgccgcCTTCTAAGGTGCTTAATTAAAAAGAAGAAGTCGAAAAAC‑TAATCGACTTTTCCCCCTGTATCGGCTGAAAAGCCT‑GTGGGGTGGCGTGTTCGGAG‑GGGAACGACATCGGTGATGAAGTGGGGCACTGCGTCGGGTTTT‑ACGAAACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGAc < 2:151674/242‑1 (MQ=255) cATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAAC‑TTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCt > 1:340462/1‑251 (MQ=255) cgccTTCTCAGGTGGGTAATTACAAAGCAGAAGTCGCAAAAC‑TTATCGCCTTTTCCCCCTGTATCGGCTGAAAGGCCT‑GTCGGGTGGCGTGTTCGGAG‑TGGAACGACACCCGTGATGAAGGGGGGCCCTGCGTCGGGTTTT‑ACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCAc < 1:103644/251‑1 (MQ=255) gccTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAAC‑TTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGGGATGAAGTGGGGCACTGCGTCGGGGGTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGGGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGAACCGTAAAGAAGGCTGTATGCACt > 2:55458/1‑251 (MQ=255) cTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAAC‑TTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACtgt > 1:553451/1‑251 (MQ=255) ggTGCGTGATTACAAAGCAGAAGTCGCAAAAC‑TTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGa > 2:500523/1‑246 (MQ=255) gtaTTAAAAAGCAAAAGTCACAAAAT‑TTACCGTCGTTCCCCCCTGTATCGGTTGTAAAGCCT‑GTCGGGTGGCGTGTTCGGAG‑GGGACCGACACCCGTGATGAGGGGGGGCCCTGGGTCGGGTTTT‑ACGAAACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCt < 2:396631/249‑1 (MQ=255) tACAAAGCAGAAGTCGCAAAAC‑TTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGGGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCCATGTGAAGATcacagctgact > 2:368165/1‑241 (MQ=255) aaGCAGAAGTCGCAAAAC‑TTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAAACGGCTGTATGCACTGTGAAGATCCCGGCCGCCttc > 2:535349/1‑247 (MQ=255) gCAGAAGTCGCAAAAC‑TTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGc > 1:173522/1‑251 (MQ=255) aGAAGTCGCAAAAC‑TTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCgg > 2:339029/1‑250 (MQ=255) aaaaC‑TTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGAGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTctg > 1:552903/1‑251 (MQ=255) aC‑TTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGGctgctg > 2:73511/1‑251 (MQ=255) acctgctttcccccttGTATCGGCTGAAAAGCGT‑GTGGGGTGGCGTGTTGGGAG‑TGGAACGACACCTTTGAGGAGGGGGGGCCCGGGGTGGGGTTTT‑ACAATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGc < 2:485485/236‑1 (MQ=255) aTCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGAAACGACATCCGGGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGCTCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGaa > 2:7912/1‑164 (MQ=255) tCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGc > 1:268739/1‑250 (MQ=255) ccgtttcccccgttatgggCTGTAAAGCCT‑G‑CAGGGGGGGGGTTGGGGGGTGGACCGACATCCGTGATGAAGGGGGGCATTGGGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATc < 1:55458/234‑1 (MQ=255) tcccccTGTATCGGCTGTAAAGCCT‑GTCGGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGGGGGGCACTGCGTGGGGGTTT‑ACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTc < 1:117270/246‑1 (MQ=255) cGGCTGAAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACGGCGTCGGGTTTT‑ACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACg < 1:339029/251‑1 (MQ=255) aaaGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAAAGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACACCGCCGATCTGAGCGCAAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATTCGTAAAGACGGCCGTATGCACTGCGAAGATCCCGGATGCATGACGGCGTGCCCGTCTGCTGGTGCAATCATTCAGCACGCTAACGGGATTgc > 2:101608/1‑250 (MQ=255) ccT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGAt > 1:351798/1‑251 (MQ=255) cT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGAtt > 2:191995/1‑251 (MQ=255) cT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGAACTGCGTCGGGGTGT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGGACGCTAACggggtgacccgcc > 1:14878/1‑241 (MQ=255) gtTCGGGG‑TGGACCGACATCCGTGATGAAGTGGGGCCCTGGGGCGGGGTTT‑ACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGaaaa < 1:326495/250‑1 (MQ=255) ttCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCCTTCAGTACGCTAACGGGATTGTCGATTTCCCGTCGGAAAACt > 2:472244/1‑251 (MQ=255) ggAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGc > 2:232512/1‑251 (MQ=255) gAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCt < 1:73511/251‑1 (MQ=255) gAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCAAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCt > 1:116020/1‑251 (MQ=255) aaCGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCtg > 1:307249/1‑251 (MQ=255) ccGACATCCGTGATGAAGTGGGGCACTGCGTGGGGTTTT‑ACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCtgt < 1:191654/250‑1 (MQ=255) caaCATCCGTGATGAGGGGGGGCCCTGCGTCGGGGTTT‑ACGAGACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCtgtg < 1:507044/249‑1 (MQ=255) acacccgtggagaAGGGGGGCCCGGCGTGGGGGTTT‑ACGAAACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTggn < 2:247925/240‑2 (MQ=255) cATCCGTGAGGAAGGGGGGCACTGCGGGGGGTTTT‑ACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGtt < 1:291940/251‑1 (MQ=255) cGTGAGGAGGTGGGGCTCGGGGTCGGGGTTT‑ACTATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTAcat < 1:82479/251‑3 (MQ=255) gatgaAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCAt > 1:146817/1‑251 (MQ=255) atgaAGTGAGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCAAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGAGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGGACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGGTACTGccat > 2:489766/1‑248 (MQ=255) gaAGGGGGGCCCTGCGTCGGGGTTG‑ACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCATTg < 1:297997/250‑1 (MQ=255) aGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCATTGCCg > 1:503532/1‑251 (MQ=255) ggggCACTGCGTCGGGGTTT‑ACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCATTGCCGGgt < 1:199223/251‑1 (MQ=255) ggCACTGCGTAGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGGGGTTACTGCATTGCCgggggg > 2:174197/1‑248 (MQ=255) ggcggcGGGTTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCATTGCCGGGTGTCCGtt < 1:470300/246‑1 (MQ=255) gTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGGGGTTACTGCATTGCCGGGGGTCCGGTTa > 2:353497/1‑250 (MQ=255) ggTTT‑ACGATACCCCAGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCATTGCCGGGTGTCCGTTTAATATTc < 1:523904/251‑1 (MQ=255) ggTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCATTGCCGGGTGTCCGTTTAATATTc > 1:339138/1‑251 (MQ=255) tt‑ACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCATTGCCGGGTGTCCGTTTAATATTCcg < 1:191995/250‑1 (MQ=255) | CTTAGTCAACATCGAGAAGGCGTAAGGGGGCGAACAGATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGC‑AACTAACTCCATCACGCCGCCTTCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAAC‑TTATCGACGTTTCCACCTGTATCGGCTGTAAAGCCT‑GTCAGGTGGCGTGTTCGGAG‑TGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTT‑ACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCATTGCCGGGTGTCCGTTTAATATTCCG > NC_000913/1550424‑1550904 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |