Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,279,012	T→C	D178D (GAT→GAC)	ghxP →	guanine/hypoxanthine transporter GhxP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,279,012	0	T	C	100.0%	175.8 / NA	49	D178D (GAT→GAC)	ghxP	guanine/hypoxanthine transporter GhxP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/29);  total (20/29)

AGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCA  >  NC_000913/4278770‑4279255                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    aGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCGGGGTGACTTCGCGAc                                                                                                                                                                                                                                             <  2:133600/251‑1 (MQ=255)      ggTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGGTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTcc                                                                                                                                                                                                                                        <  1:438318/251‑1 (MQ=255)      ggTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCtt                                                                                                                                                                                                                                          <  2:64564/249‑1 (MQ=255)                 aaCATATTAGCGTACCTGTCGCCCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTGTAGCTGGGTGACTTCGCGACCTTCCCGGGGGTTATg                                                                                                                                                                                                                             >  2:65130/1‑251 (MQ=255)                  aCATATTAGCGTACCTGTCGAGCTGGTTGCCCTGCTCCTGATCGGTGTGCTGCTTACGGTAATTTCTGCCACGGGAATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGAGATTGGTATCGGTCTGTTCCTGATGCTCATTGCCGCTAACGGTTTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGCCCTTCCCGGTggctatgt                                                                                                                                                                                                                            >  2:510563/1‑244 (MQ=255)                   cATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTc                                                                                                                                                                                                                           <  1:508527/251‑1 (MQ=255)                          gCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTa                                                                                                                                                                                                                    <  1:528207/251‑1 (MQ=255)                                       gcTGGGTGCCGGGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGGCACTGGTAGGTCatacctaac                                                                                                                                                                                                       <  2:285763‑M1/251‑10 (MQ=255)                                         tGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacg                                                                                                                                                                                                      >  1:522544‑M1/1‑240 (MQ=255)                                           ggTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgaca                                                                                                                                                                                                   <  2:557426‑M1/251‑14 (MQ=255)                                              gCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaac                                                                                                                                                                                                 >  2:65255‑M1/1‑235 (MQ=255)                                               ccGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTTCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacag                                                                                                                                                                                               <  1:467347‑M1/251‑18 (MQ=255)                                               ccGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacag                                                                                                                                                                                               <  1:182124‑M1/251‑18 (MQ=255)                                                                        tACGGTATTTTCGGCCCCGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCCCGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTACCGGTGTCGGTCGGGTGATTAAAACCCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGCCTTCGAGACCTCCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggt                                                                                                                                                                       <  2:361506‑M1/250‑42 (MQ=255)                                                                           ggTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCGCGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGGCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtg                                                                                                                                                                   <  2:232024‑M1/251‑46 (MQ=255)                                                                              aaTTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaa                                                                                                                                                                 <  2:299162‑M1/250‑48 (MQ=255)                                                                               aTTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatc                                                                                                                                                               <  1:250720‑M1/251‑50 (MQ=255)                                                                                                  gTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGCCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaacggtagtgaatcgtttgcttttaactgggg                                                                                                                                             >  2:562136‑M1/1‑183 (MQ=255)                                                                                                    aGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggatt                                                                                                                                          <  1:578621‑M1/251‑71 (MQ=255)                                                                                                         gATTTTGCGCAACTTGCCTCACGTTGTGGTGCCCGGCACGGTGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAGCGGTGTCTGTCTGGTGAGTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCGGGGTGGCTTCGCTACCTTCCCGGTGGTTATTTCACTGGTAGGTCataccttacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttca                                                                                                                                     <  2:57532‑M1/251‑76 (MQ=255)                                                                                                          aTTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTAatacctaacgccaacaggaaaaacaaccataaccaaaaggtagtgaatcgtttgcttttaactggggattgttc                                                                                                                                      >  1:515783‑M1/1‑175 (MQ=255)                                                                                                                cgcAACTTGCCTCACTGTTTGTCGCTCGGCACGGGTAGTGGTATCGGTCTTTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGTTCTGCCCGTTGCGCTGGGTGACTTCTCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaatgtagtgaatcgtttgcgtttaacgggggattgttcatgtggct                                                                                                                              <  2:515783‑M1/251‑83 (MQ=255)                                                                                                                  cAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgtt                                                                                                                            >  1:362772‑M1/1‑167 (MQ=255)                                                                                                                   aaCTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttg                                                                                                                           >  1:15178‑M1/1‑166 (MQ=255)                                                                                                                    aCTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgc                                                                                                                          <  1:445579‑M1/251‑87 (MQ=255)                                                                                                                         ccTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgt                                                                                                                     <  2:522544‑M1/251‑92 (MQ=255)                                                                                                                           tCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgt                                                                                                                                                             >  1:351363‑M1/1‑158 (MQ=255)                                                                                                                           tCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgt                                                                                                                                                             <  2:351363‑M1/209‑52 (MQ=255)                                                                                                                            cACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgtttt                                                                                                                  >  2:492764‑M1/1‑157 (MQ=255)                                                                                                                             aCGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttg                                                                                                                 >  2:257412‑M1/1‑156 (MQ=255)                                                                                                                                    ggCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgtta                                                                                                          <  1:257412‑M1/251‑103 (MQ=255)                                                                                                                                     gcgcACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaa                                                                                                         <  1:493546‑M1/251‑104 (MQ=255)                                                                                                                                                  ggATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggaggg                                                                                            >  1:542016‑M1/1‑135 (MQ=255)                                                                                                                                                    aTTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGGCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGGCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggcggcgttgcggtgttttgaaggttaagactgggggaggg                                                                                            >  2:301897‑M1/1‑133 (MQ=255)                                                                                                                                                          aTCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTTCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTTGTAGGTCatacctaacgacaacatgaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtgacgttgcggtgttttgaatgttaagactgtgggagggagaatct                                                                                     <  1:543489‑M1/250‑124 (MQ=255)                                                                                                                                                                cTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggc                                                                                >  1:539946‑M1/1‑121 (MQ=255)                                                                                                                                                                        gctgctCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggaaccgcc                                                                      <  2:362772‑M1/251‑139 (MQ=255)                                                                                                                                                                           gctCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggaaccgcct                                                                     <  2:542016‑M1/249‑140 (MQ=255)                                                                                                                                                                             tCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtt                                                                                                                                                            >  2:253964‑M1/1‑108 (MQ=255)                                                                                                                                                                             tCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtt                                                                                                                                                            <  1:253964‑M1/160‑53 (MQ=255)                                                                                                                                                                                 tGCCCCTAACGGTGTGGGTTTGGTGATTAAAACCCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatccccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggctttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggaaccgcctctggtat                                                              <  1:492764‑M1/250‑147 (MQ=255)                                                                                                                                                                                                  gTCTGGTGATTAAAAACCCGCTTGTTGGTCTGCCCGTGGCGCGGGGTGACTTCGCGACCTTCCGGGGGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaacccatacccaaaaggtagtgaatcgtttgcttttaacgggggattgttcaggtggcgttgggtttttttgaatgttaagactgtgggggggagaatctgtggcggaaaccgcctctggtatagggggaggcgaagatag                                            <  1:65255‑M1/251‑165 (MQ=255)                                                                                                                                                                                                         gATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggaaccgcctctggtatagggggaggcgaagatagcattatt                                     >  1:80595‑M1/1‑80 (MQ=255)                                                                                                                                                                                                                       ttGATGGTCTGCCCGTTGCGCTGGGTGACCTCGCAACCTTCCCGGTGTTTATGTCACTGGTAGGTCatacctaacgacacaagaaaaaaaaaccatcccaaaaaggtagtgaatcgtttgcttttacctggggattgttcaggtggcgttgcggttttttgaatgttaagtctttgggagggagaatctgtggcaggaaccgcctctggtatagggggaggcgaagatagcattatttcctctccctct                         <  1:65130‑M1/249‑184 (MQ=255)                                                                                                                                                                                                                          aTGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggaaccggctcttgtattgggggaggggaagatagcattatttactctccctcttcttc                    >  2:89386‑M1/1‑63 (MQ=255)                                                                                                                                                                                                                            ggtcgcccctttgcgctgggtgACTTCGCGCCCTTCCGGGTGTTTATGTCACTGGTAGGTCatacctaacgacaacagaaaaaaaaaccataccaaaaaggtagtgaatcttttgtttttaactggggattgttcaggtggcgttgcggtgttttgaatgtaaagactgtgggagggagaatcggtgcagggaaccgcctctggtatagggggaggcgaagatagcattatttcctctccctcttcttcg                   <  1:89386‑M1/240‑190 (MQ=255)                                                                                                                                                                                                                                          gcTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggaaccgcctctgggatagggggaggcgaagatagcattatttcctctccctcttcttcgctgtaccagataacc    >  1:239280‑M1/1‑47 (MQ=255)                                                                                                                                                                                                                                           cTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggaaccgcctctggtatagggggaggcgaagatagcattatttcctctccctcttcttcgctgtaccagataaccg   >  1:141729‑M1/1‑46 (MQ=255)                                                                                                                                                                                                                                            tGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggaaccgcctctggtatagggggaggcgaagatagcattatttcctctccctcttcttcgctgtaccagataaccgg  >  1:151174‑M1/1‑45 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    AGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCA  >  NC_000913/4278770‑4279255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑