Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	498,116	C→T	P21L (CCT→CTT)	hemH →	ferrochelatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	498,116	0	C	T	98.6%	264.8 / ‑6.7	73	P21L (CCT→CTT)	hemH	ferrochelatase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (42/29);  total (43/30)
Fisher's exact test for biased strand distribution p-value = 4.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCGCACAAGCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGG  >  NC_000913/497885‑498362                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       cccGCACAAGCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTTAAGCGGTAAAACGCTAt                                                                                                                                                                                                                                     <  2:450038/251‑1 (MQ=255)          gCAAACTTATCAGCCATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTACTGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGCAGCGGTAAAACGCTATCTGAAACa                                                                                                                                                                                                                             >  2:126696/1‑250 (MQ=255)           cAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAAt                                                                                                                                                                                                                           >  2:167932/1‑251 (MQ=255)              aCTTAGCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAAtttt                                                                                                                                                                                                                        <  2:38601/251‑1 (MQ=255)                ttATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAAttttt                                                                                                                                                                                                                       >  1:412206/1‑250 (MQ=255)                ttATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAAttttt                                                                                                                                                                                                                       >  2:474609/1‑250 (MQ=255)                    cAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCg                                                                                                                                                                                                                  >  2:150794/1‑251 (MQ=255)                     aGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAAttt                                                                                                                                                                                                                         >  2:25672/1‑243 (MQ=255)                     aGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAAttt                                                                                                                                                                                                                         <  1:25672/243‑1 (MQ=255)                       cAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACa                                                                                                                                                                                                               <  1:119433/251‑1 (MQ=255)                           ctcAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGAcg                                                                                                                                                                                                           >  2:535888/1‑251 (MQ=255)                             cAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGAcgcg                                                                                                                                                                                                         >  1:104289/1‑251 (MQ=255)                                  cGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGtt                                                                                                                                                                                                    >  2:484963/1‑251 (MQ=255)                                   ggATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTTAAACCGGTATCCTGCTGACAAACCTGGGTACGCCCGATGCCCCCACCCATTAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACCTACGCGTGGTTg                                                                                                                                                                                                   >  2:555272/1‑251 (MQ=255)                                       attcattcGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATAc                                                                                                                                                                                               >  2:194274/1‑251 (MQ=255)                                          cattcGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCCGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTc                                                                                                                                                                                            <  1:546633/251‑1 (MQ=255)                                              cGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCttaccg                                                                                                                                                                                        >  1:590153/1‑246 (MQ=255)                                                gCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGgtt                                                                                                                                                                                      <  1:484963/251‑1 (MQ=255)                                                       aCAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACTCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTAtgg                                                                                                                                                                               >  1:528392/1‑251 (MQ=255)                                                          aaaaCCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTAtggtgg                                                                                                                                                                            >  1:256695/1‑251 (MQ=255)                                                              ccTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCAt                                                                                                                                                                        <  1:277270/251‑1 (MQ=255)                                                              ccTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCAt                                                                                                                                                                        <  2:104289/251‑1 (MQ=255)                                                                     cTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTgcg                                                                                                                                                                 >  1:384660/1‑251 (MQ=255)                                                                           tATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCgcggcgg                                                                                                                                                           >  1:509412/1‑250 (MQ=255)                                                                                cTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTAt                                                                                                                                                                                 >  2:502089/1‑224 (MQ=255)                                                                                cTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTAt                                                                                                                                                                                 <  1:502089/224‑1 (MQ=255)                                                                                        gATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCt                                                                                                                                               <  1:194274/250‑1 (MQ=255)                                                                                                       aGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGtgtg                                                                                                                               <  2:256695/251‑1 (MQ=255)                                                                                                       aGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGtgtg                                                                                                                               <  1:150794/251‑1 (MQ=255)                                                                                                       aGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGGTATGGTTGCCATTGCTGCGCGGCGGGATTTTGCCGCTGCGCTCGCCGCGtgtg                                                                                                                               >  2:52845/1‑251 (MQ=255)                                                                                                           aTTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGACGGAAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGAACGCCCGATGCCCCCCCACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCg                                                                                                                            >  1:377921/1‑250 (MQ=255)                                                                                                             tAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGaa                                                                                                                          <  1:58941/250‑1 (MQ=255)                                                                                                                ttcttcttcCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTGAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGGGGTTGATACCTCACGGGTGTTATGGTGGCCATTTCTGCGGGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTg                                                                                                                      >  2:148957/1‑251 (MQ=255)                                                                                                                 tcttcttcCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTg                                                                                                                      <  2:423151/250‑1 (MQ=255)                                                                                                                     cttcCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTCTGc                                                                                                                 >  2:585530/1‑251 (MQ=255)                                                                                                                      ttcCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTAAGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGcc                                                                                                                >  2:389388/1‑251 (MQ=255)                                                                                                                        ccTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCTTGTGGCGAAGCTGTATGCct                                                                                                               <  2:501105/250‑1 (MQ=255)                                                                                                                        ccTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGCAGCTGTATGCctc                                                                                                              >  2:1345/1‑251 (MQ=255)                                                                                                                        ccTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCctc                                                                                                              <  1:167932/251‑1 (MQ=255)                                                                                                                          tCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCtctg                                                                                                            >  1:528070/1‑251 (MQ=255)                                                                                                                            aCTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCtctgtt                                                                                                          >  2:81275/1‑250 (MQ=255)                                                                                                                                ttCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCtgg                                                                                                       <  2:412206/250‑1 (MQ=255)                                                                                                                                         aaTTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTg                                                                                             <  2:335754/251‑1 (MQ=255)                                                                                                                                          aTTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGAAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGCTACCTCACGGTTGTTATGGTGGCCATTTCGGAGCGGCGGGATTTTGCCGCTGCGGCCCCCGCGGGTGGCCGAGCTGTATGCCCCTGGCTGGATGGAAggggg                                                                                            >  2:42539/1‑248 (MQ=255)                                                                                                                                          aTTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGGATCCTGCTGGCAAACCTGGGGACGCCCGATGCCCCCACACTTGAAGCGGGAAAACGCTATCTGAAACAATTTTTAAGCGACAGGCGCGTGGTTGCTACCTCACGGTTTTTATGGTTGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTgg                                                                                            >  2:377002/1‑251 (MQ=255)                                                                                                                                                aacaaGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTcgcc                                                                                      <  1:568250/251‑1 (MQ=255)                                                                                                                                                  caaGTTGAAGCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGCAACCTGCAGGGAAAACAGGGCAAGCCCGACGCCCCCACACTTGACGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAAACGCGTGGGTGATACCTCACGGGTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCACCGCGTGTCGCGAAGCCGTATGCCTCCGTCTGGGTTGAAGGTGGCtagcagc                                                                                    >  2:87479/1‑245 (MQ=255)                                                                                                                                                    aGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGGGGCGAAGCTGTATGCCTCTGTCTGGATGGCAGGGGGCTCGCCGCTg                                                                                  >  2:240647/1‑251 (MQ=255)                                                                                                                                                    aGTTGAATCGATAAGAGGCGGTAATGCGTCAGAATAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGGTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGCGTGGCGAAGCTGTATGCCTCTGTCTGGGTGGCAGGCGGCTCGCCGCTg                                                                                  >  2:223518/1‑251 (MQ=255)                                                                                                                                                     gTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTg                                                                                  >  1:552909/1‑250 (MQ=255)                                                                                                                                                          aTCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGACGGTGGCTCGCCGCTGATGGtt                                                                            >  1:513/1‑251 (MQ=255)                                                                                                                                                            cGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGttt                                                                           <  1:126696/250‑1 (MQ=255)                                                                                                                                                             gATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAATCAATTTTTAAGCTACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTAc                                                                         <  2:129856/251‑1 (MQ=255)                                                                                                                                                                aaGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGAGTGTTTACAgc                                                                      >  2:213414/1‑251 (MQ=255)                                                                                                                                                                  gagGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACTCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAgccg                                                                    <  2:528392/251‑1 (MQ=255)                                                                                                                                                                  gagGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCt                                                                                                                                                                                             <  1:419641/130‑1 (MQ=255)                                                                                                                                                                  gagGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGCACGCCCGATGCCCCCACACTTTAAGCGGTAAAACGCTATCTGAAAAAATTTTTAAGCGACAGACGCGTGGTTGATACCt                                                                                                                                                                                             >  2:419641/1‑130 (MQ=255)                                                                                                                                                                        ntAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGGGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTAGGCCTCTGTCTGGATGGAAGGGGGTTCGCCGCTAATGGTTTCCAGCCGCCAGc                                                               >  2:263236/2‑250 (MQ=255)                                                                                                                                                                           aTGCGACAGACTAAAAACGGCATCCTGCTGGCAAACCTGGGTAAGCCCGATGCCCCCCCACTTGAAGCGGTAAAACGATATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCAATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGCGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAAc                                                            >  2:499083/1‑250 (MQ=255)                                                                                                                                                                                 cAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCGGgc                                                     >  2:310351/1‑251 (MQ=255)                                                                                                                                                                                 cAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGgc                                                     <  2:130335/251‑1 (MQ=255)                                                                                                                                                                                  aGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGgcg                                                    >  1:499339/1‑251 (MQ=255)                                                                                                                                                                                     cTAAAACCGGAATCCTGATGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGgcgc                                                   >  2:447703/1‑249 (MQ=255)                                                                                                                                                                                       caaaCCGCTATCCTGCTCGCAACCCTGGGTACGCCCGACCCCCCCACACTTGAAGCGGCAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGCGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGACGCTGTATGCCTCTGTCTGGATGGAAGGTGGCCCGCCGCTGATGGTTTACAGCCGCCAGCACCAGCAGGCGCTGGc                                               >  2:293818/2‑251 (MQ=255)                                                                                                                                                                                       aaaaCCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGCAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGc                                               <  1:1345/251‑1 (MQ=255)                                                                                                                                                                                       aaaaCCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGc                                               <  2:509412/251‑1 (MQ=255)                                                                                                                                                                                                aTCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGttt                                      <  2:150825/251‑1 (MQ=255)                                                                                                                                                                                                     gctgGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTAcc                                   <  2:377921/249‑1 (MQ=255)                                                                                                                                                                                                                       gCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTgg               >  2:294153/1‑251 (MQ=255)                                                                                                                                                                                                                           gATGCCCCCACACCTGAAGCGGTAAACCGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAAt           <  1:377002/251‑1 (MQ=255)                                                                                                                                                                                                                              gCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATg          >  1:177239/1‑249 (MQ=255)                                                                                                                                                                                                                                  ccACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTAc    <  2:552909/251‑1 (MQ=255)                                                                                                                                                                                                                                    acacTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACgg  >  1:224168/1‑251 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       CCCGCACAAGCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGG  >  NC_000913/497885‑498362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑