Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,468,768	C→T	W362* (TGG→TGA)	chiA ←	endochitinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,468,768	0	C	T	100.0%	246.8 / NA	65	W362* (TGG→TGA)	chiA	endochitinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/36);  total (29/36)

CATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGA‑TCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTT  >  NC_000913/3468526‑3469014                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                           cATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGtt                                                                                                                                                                                                                                                   <  1:281355/251‑1 (MQ=255)          tGATCGACAGAAGTTTTATAATGGGCATCGTTATAATCTCCGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTaaa                                                                                                                                                                                                                                           <  2:191135/250‑1 (MQ=255)                  aGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACTCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCAt                                                                                                                                                                                                                                  <  2:173371/251‑1 (MQ=255)                  aGAAGTTTTAAAATGTGCATCGTTTTAATCTACGAGGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGGCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACGTTACCAt                                                                                                                                                                                                                                  <  2:490656/251‑1 (MQ=255)                           ataATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATa                                                                                                                                                                                                                          >  1:590082/1‑250 (MQ=255)                              atgttcatagtaataATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGTAAACTATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTTAACATTACCATTGAAAATAACAt                                                                                                                                                                                                                      <  2:252974/239‑1 (MQ=255)                              aTGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACAt                                                                                                                                                                                                                      <  2:533093/251‑1 (MQ=255)                                gtgCATCGTTATAATCTACGATGCCGTCCGCGCGGTACGTTTTGCTGGCCTGGAAACAATCCGCAACAAAGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATgg                                                                                                                                                                                                                    <  1:387959/251‑1 (MQ=255)                                    aTCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTaa                                                                                                                                                                                                                 >  2:107822/1‑250 (MQ=255)                                       gTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAAttt                                                                                                                                                                                                             <  1:107822/251‑1 (MQ=255)                                          ataATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGGGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGc                                                                                                                                                                                                          >  2:529682/1‑251 (MQ=255)                                           taATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGACGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCAAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCt                                                                                                                                                                                                         >  1:368673/1‑251 (MQ=255)                                            aaTCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCt                                                                                                                                                                                                         >  1:340091/1‑250 (MQ=255)                                                 aCGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCCTCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTc                                                                                                                                                                                                   <  2:170599/251‑1 (MQ=255)                                                 aCGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTc                                                                                                                                                                                                   >  1:456403/1‑251 (MQ=255)                                                 aCGATGCTGTCCGCACTGTACGCTTTGCTGGCCTGGAATCCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCATTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCt                                                                                                                                                                                                     <  2:67379/249‑1 (MQ=255)                                                         gTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGc                                                                                                                                                                                           >  1:75186/1‑251 (MQ=255)                                                              cgttgtccGCTTGGCTGGCCGGGAAACCTCCCGCTGTAATTACGCCGCCGTTATCAATTTCGCAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCTATACGCCATGTGTTATTGGATTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑TCGTTCTTTTAATCATTACCATTGAAAATAACATGGTAAATTTCGCTTCCTTCCTGGCCGCTTCAc                                                                                                                                                                                      <  2:153663/244‑1 (MQ=255)                                                               gcTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCGATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACg                                                                                                                                                                                     <  2:540605/251‑1 (MQ=255)                                                                    aCGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGc                                                                                                                                                                                >  1:273557/1‑251 (MQ=255)                                                                         ttGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCt                                                                                                                                                                                          <  1:444370/236‑1 (MQ=255)                                                                         ttGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAAt                                                                                                                                                                             <  1:106323/249‑1 (MQ=255)                                                                         ttGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATg                                                                                                                                                                            <  2:49885/250‑1 (MQ=255)                                                                         ttGCTGGACTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCt                                                                                                                                                                                          >  2:444370/1‑236 (MQ=255)                                                                          tGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGa                                                                                                                                                                           <  1:226396/250‑1 (MQ=255)                                                                                     aaaCCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAc                                                                                                                                                              <  1:536867/251‑1 (MQ=255)                                                                                       aCCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑Gc                                                                                                                                                           >  1:171797/1‑251 (MQ=255)                                                                                            ccGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATc                                                                                                                                                       <  1:46591/250‑1 (MQ=255)                                                                                               caacaaTGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTTCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATCCGCt                                                                                                                                                   <  2:340091/251‑1 (MQ=255)                                                                                                 acaaTGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCa                                                                                                                                                                               <  2:29730/223‑1 (MQ=255)                                                                                                 acaaTGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCa                                                                                                                                                                               >  1:29730/1‑223 (MQ=255)                                                                                                 acaaTGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATCCGCTgg                                                                                                                                                 >  1:499319/1‑251 (MQ=255)                                                                                                   aaTGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATc                                                                                                                                                       <  2:135822/243‑1 (MQ=255)                                                                                                   aaTGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATc                                                                                                                                                       >  1:135822/1‑243 (MQ=255)                                                                                                       aCGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATCCGCTGGTGTTg                                                                                                                                            >  2:280662/1‑250 (MQ=255)                                                                                                            gccgTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATCCGCTGGTGTTGATGGcc                                                                                                                                      >  2:12576/1‑250 (MQ=255)                                                                                                              cgTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGCGTAA‑TCGTTAAC‑GCTATCCGCTGGCGTTGATGGCTCg                                                                                                                                    >  2:71370/1‑251 (MQ=255)                                                                                                                      aTTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGa                                                                                                                                                                           <  1:482461/206‑1 (MQ=255)                                                                                                                           acaGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAg                                                                                                                       <  2:368673/251‑1 (MQ=255)                                                                                                                            caggtatgcGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAgn                                                                                                                      <  2:258985/243‑2 (MQ=255)                                                                                                                             aGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGtt                                                                                                                     >  2:208928/1‑251 (MQ=255)                                                                                                                                             gTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGttt                                                                                                     >  2:178782/1‑251 (MQ=255)                                                                                                                                                 tGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGCACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATCCGCTGGTGGTGATGGCTCGCTGTTATCAGGAGTTGGGGTCACCGGTGTTTCCgg                                                                                                 >  1:200438/1‑251 (MQ=255)                                                                                                                                                      cAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAAt                                                                                                                                                                             >  2:89843/1‑172 (MQ=255)                                                                                                                                                      cAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAAt                                                                                                                                                                             <  1:89843/172‑1 (MQ=255)                                                                                                                                                      cAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGt                                                                                              <  2:75186/249‑1 (MQ=255)                                                                                                                                                      cAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTg                                                                                            <  2:30187/251‑1 (MQ=255)                                                                                                                                                       aaTTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTgg                                                                                           >  1:223372/1‑251 (MQ=255)                                                                                                                                                           ccGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGA‑Tc                                                                                       <  1:164832/250‑1 (MQ=255)                                                                                                                                                              cAGCGGAAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTCGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGA‑TCAAcc                                                                                   >  2:525803/1‑251 (MQ=255)                                                                                                                                                                  ggTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGA‑TCAACCGGAg                                                                               <  1:280662/251‑1 (MQ=255)                                                                                                                                                                    gaGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTGTTCAGCGCTGGTACCCCGGGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCGTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGA‑TCAACCGGAGtn                                                                             <  2:273557/250‑2 (MQ=255)                                                                                                                                                                       cTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGCGTTGGA‑TCAACCGGAGTcac                                                                           >  2:563953/1‑250 (MQ=255)                                                                                                                                                                           aCGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTGAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGGAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATCCGCTGGTGTTGATAGCTCGCTGTTAGCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGA‑TCAACCGGAGTCCCAGCAc                                                                      <  2:3021/251‑1 (MQ=255)                                                                                                                                                                                      gCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATCCGCTGGTGTTg                                                                                                                                            <  1:478817/171‑1 (MQ=255)                                                                                                                                                                                      gCCATGGGTTATTGGAGTTTTCAGCGATGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATCCGCTGGTGTTg                                                                                                                                            >  2:478817/1‑171 (MQ=255)                                                                                                                                                                                             gTTATTGGAGTTTTCAGAGCTGGTACCCAGTGGGCAATCTTTAGACACAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGTGGTCACCGGTGTTTCCGGAGTTGGA‑TCAACCGGAGTCACAGCACCGCCATTATTATCCGGCt                                                    >  2:20816/1‑251 (MQ=255)                                                                                                                                                                                                       ttttCAGCGCTGGTCCCCCGTGGGCAATCTTTAAACCCAACCCATCAG‑GCGTTCTTGTAAACATTCCCATTGAAAATAACATGGTAATTTTCGCTCCCTTCCTGGCCGCTCCACGCTTGAAATGAGTAA‑TCGTTAAC‑GCTACCCGCTGGTTTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCCCCGGTTTTTCCGGGGTTGGA‑TCAACGGGAGTCAAAGCACCGCCATTATTATCCGGCTTAACGGAAc                                           <  1:146321/250‑1 (MQ=255)                                                                                                                                                                                                       ttttCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGA‑TCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAAc                                           >  1:296881/1‑250 (MQ=255)                                                                                                                                                                                                                      ccccGTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGA‑TCAACCGTAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTAcc                           >  1:353363/1‑251 (MQ=255)                                                                                                                                                                                                                          gTGGGCAATCTTTAGACCCAACCCATCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGA‑TCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATAc                       >  2:221644/1‑251 (MQ=255)                                                                                                                                                                                                                               cAATCTTTAGACCCAACCCAT‑AGTGCTTTCTTGTAAACATCCCCATTGAAAAAAACATGGTAATTTTCGCTCCCTTCCTGGCCTCCCCCCGTTTGCAAGGAGTAT‑TGTTTAAC‑GCTTTGCGCGGGTGTTGTGGGTTCGCTTTTATCGGGGGTGGGGGTCCCCGTTGTTGCCGGA‑TTGGAACCAACCGGAGTCCCAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCt                  <  2:223372/251‑1 (MQ=255)                                                                                                                                                                                                                               aaattttaaGACCCACCCCATCAG‑GCGTTTTTGAAAAAATCACCATGAAAAATAACATGTTAAATTCCGTTCCTTTCCGGGCCGCTCCACGCTTGCAGTGAGTAA‑TC‑TTAACCGTTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTGGGGGCCACCGGTGTTCCCGGATTTGAA‑TCAACCGGGGTCCCAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCt                  <  2:156163/243‑1 (MQ=255)                                                                                                                                                                                                                                          cccaacccaTCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGA‑TCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAg                                      >  1:138174/1‑220 (MQ=255)                                                                                                                                                                                                                                               cccaTCAG‑CCGTTCTTGAAAACTTTTCCATTGAAAATAACTTGGAAAATGGCGCTCCCTTCCTGCCGGCCCCCCGCTTGCAATG‑GTAAATC‑TTAACCGCTATCCGCTGGGGTGTATGCCCGGCTGTTATCCGTATTTGGGGTCCCCGTTTTTCCCGGAGTGGAA‑CCAGCCGGAGTCACAGCCCCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTgttgccgtt  <  1:97473/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                           CATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAG‑GCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAA‑TCGTTAAC‑GCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGA‑TCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTT  >  NC_000913/3468526‑3469014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑