Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,449,779	G→A	S35S (AGC→AGT)	rpsC ←	30S ribosomal subunit protein S3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,449,779	0	G	A	100.0%	278.7 / NA	70	S35S (AGC→AGT)	rpsC	30S ribosomal subunit protein S3
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/40);  total (30/40)

CAGTTTTGCGTCCAGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCGCTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGT  >  NC_000913/3449539‑3450020                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  cAGTTTTGCGTCCAGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGt                                                                                                                                                                                                                                         >  2:543897/1‑251 (MQ=255)     tttGCGTCCAGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGt                                                                                                                                                                                                                                      <  2:487839/250‑1 (MQ=255)      ttGCGTCCAGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTc                                                                                                                                                                                                                                     >  1:8916/1‑250 (MQ=255)      ttGCGTCCAGTGCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTc                                                                                                                                                                                                                                     <  2:239429/250‑1 (MQ=255)        gCGTCCAGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAgn                                                                                                                                                                                                                                  <  2:265605/251‑2 (MQ=255)            ccAGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAAt                                                                                                                                                                                                                              >  2:173588/1‑251 (MQ=255)             cAGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAAtt                                                                                                                                                                                                                             >  1:557838/1‑251 (MQ=255)             cAGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAAtt                                                                                                                                                                                                                             <  1:513720/251‑1 (MQ=255)                  cAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTg                                                                                                                                                                                                                        <  2:240085/251‑1 (MQ=255)                     gCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGgtg                                                                                                                                                                                                                     <  1:543897/251‑1 (MQ=255)                      cTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGgtgt                                                                                                                                                                                                                    >  2:312994/1‑251 (MQ=255)                       ttACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGtt                                                                                                                                                                                                                   >  2:421513/1‑251 (MQ=255)                        tACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGtt                                                                                                                                                                                                                   <  2:493300/250‑1 (MQ=255)                                    cGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGt                                                                                                                                                                                                       >  1:216134/1‑250 (MQ=255)                                     gATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTag                                                                                                                                                                                                     <  2:557838/251‑1 (MQ=255)                                          tGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGtt                                                                                                                                                                                                 <  2:3533/250‑1 (MQ=255)                                           gagcTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGGTACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTcc                                                                                                                                                                                               <  2:39495/248‑1 (MQ=255)                                                  gCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGtt                                                                                                                                                                                         <  1:168839/250‑1 (MQ=255)                                                     ggAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGCTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTAc                                                                                                                                                                                     <  1:325356/251‑1 (MQ=255)                                                          gCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATAc                                                                                                                                                                                >  1:172315/1‑251 (MQ=255)                                                           ccAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCAATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATAcc                                                                                                                                                                               <  2:213501/251‑1 (MQ=255)                                                            cAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATAccc                                                                                                                                                                              <  2:173969/251‑1 (MQ=255)                                                             aGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATAccc                                                                                                                                                                              >  1:118195/1‑250 (MQ=255)                                                                      cGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATAc                                                                                                                                                                    <  1:42216/251‑1 (MQ=255)                                                                        cTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATAcc                                                                                                                                                                   >  1:84599/1‑250 (MQ=255)                                                                         tACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCAt                                                                                                                                                                 >  1:234376/1‑251 (MQ=255)                                                                         tACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCAt                                                                                                                                                                 >  1:292329/1‑251 (MQ=255)                                                                          aCGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCAtt                                                                                                                                                                <  1:64167/251‑1 (MQ=255)                                                                            gACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAg                                                                                                                                                              <  2:501346/251‑1 (MQ=255)                                                                                  aCGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGt                                                                                                                                                         <  1:282059/250‑1 (MQ=255)                                                                                   cGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTAc                                                                                                                                                       >  1:398643/1‑251 (MQ=255)                                                                                      aGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACttt                                                                                                                                                    <  1:249781/251‑1 (MQ=255)                                                                                       gTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTc                                                                                                                                                   <  1:173588/251‑1 (MQ=255)                                                                                         ttttCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCt                                                                                                                                                  <  1:249849/250‑1 (MQ=255)                                                                                            tCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGAc                                                                                                                                               <  2:292329/250‑1 (MQ=255)                                                                                              tACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGCGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGAccc                                                                                                                                             >  2:418016/1‑250 (MQ=255)                                                                                                   cTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCt                                                                                                                                       >  1:53390/1‑251 (MQ=255)                                                                                                     tCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCGGACCCATTGCTa                                                                                                                                      <  1:421513/250‑1 (MQ=255)                                                                                                      cACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGt                                                                                                                                    <  2:122554/251‑1 (MQ=255)                                                                                                         cTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGtctc                                                                                                                                 >  1:408874/1‑251 (MQ=255)                                                                                                                     aaaCGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGc                                                                                                                     <  2:234376/250‑1 (MQ=255)                                                                                                                        cGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGAt                                                                                                                  <  1:315910/251‑1 (MQ=255)                                                                                                                         gATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATc                                                                                                                 >  1:168148/1‑251 (MQ=255)                                                                                                                                gggCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGacac                                                                                                           >  1:566276/1‑250 (MQ=255)                                                                                                                                  gCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCTCAAACCGGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAAc                                                                                                        <  1:360752/251‑1 (MQ=255)                                                                                                                                   cGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAAcc                                                                                                       >  1:288155/1‑251 (MQ=255)                                                                                                                                    gAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAAcc                                                                                                       >  1:195686/1‑250 (MQ=255)                                                                                                                                                tGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGc                                                                                          >  1:505301/1‑251 (MQ=255)                                                                                                                                                   ttACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTgg                                                                                       >  2:442506/1‑251 (MQ=255)                                                                                                                                                           aTGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTc                                                                               >  1:277130/1‑251 (MQ=255)                                                                                                                                                                 ttAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGAt                                                                          <  2:358242/250‑1 (MQ=255)                                                                                                                                                                   aGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGc                                                                        <  2:8916/250‑1 (MQ=255)                                                                                                                                                                   aGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAAGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCg                                                                       >  1:182308/1‑251 (MQ=255)                                                                                                                                                                     ccGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGAt                                                                     <  2:417904/251‑1 (MQ=255)                                                                                                                                                                       ggACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCt                                                                   <  1:186955/251‑1 (MQ=255)                                                                                                                                                                        gACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTAAGGATGCGAtatg                                                                  >  1:573163/1‑248 (MQ=255)                                                                                                                                                                            cTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACg                                                              >  1:194331/1‑251 (MQ=255)                                                                                                                                                                                 tAACGATACGAGATACGGCCGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCCGGATGCGATCTGCCCGACCtt                                                         >  2:161492/1‑251 (MQ=255)                                                                                                                                                                                            gaTACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCATACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGc                                              <  2:172315/251‑1 (MQ=255)                                                                                                                                                                                                        ttAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTa                                                                                                                                                                                                      >  2:384984/1‑87 (MQ=255)                                                                                                                                                                                                        ttAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTa                                                                                                                                                                                                      <  1:384984/87‑1 (MQ=255)                                                                                                                                                                                                          aGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATTATGCGCTTCAt                                <  1:481474/251‑1 (MQ=255)                                                                                                                                                                                                               gTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCg                           >  1:14330/1‑251 (MQ=255)                                                                                                                                                                                                                  ccTTAGTCAGGTACTGACGTCCTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGCGTTCAATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTACGCTTCCGGCTGCGATCTGCACGCCCTTTTGCCCGCGGCATAATGCGATTCCTGCTCGGGc                        >  2:348000/1‑251 (MQ=255)                                                                                                                                                                                                                     tAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCt                      <  2:408874/250‑1 (MQ=255)                                                                                                                                                                                                                      aGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTc                    <  2:97558/251‑1 (MQ=255)                                                                                                                                                                                                                       gTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCg                   <  1:54296/251‑1 (MQ=255)                                                                                                                                                                                                                       gTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAAGTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCg                   <  2:194331/251‑1 (MQ=255)                                                                                                                                                                                                                           ggTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGTCCTTCGTCTa               <  2:505466/251‑1 (MQ=255)                                                                                                                                                                                                                                         ttAAAATCACTGTCCAGGTTGTCAGCGAATTCCTGGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGt  <  2:118195/250‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  CAGTTTTGCGTCCAGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCGCTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGT  >  NC_000913/3449539‑3450020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑