Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4350CT34.4% 38.0 / 12.3 32intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (3/18);  new base T (11/0);  total (14/18)
Fisher's exact test for biased strand distribution p-value = 2.82e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGACAAATACAAAAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGA‑CAGAGAT‑TGTCGCTAACTTTACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGAC  >  NC_000913/3206188‑3206681
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                          
aaaaCTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAg                                                                                                                                                                                                                                                         <  1:20872/251‑1 (MQ=255)
            gtgAACGATTTCTACCAGTGCCTGCATGTCCTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTAGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTTATTACATATTCACGGTGGCAAAAAATATAAAACCACACTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGACAAATACaaa                                                                                                                                                                                                                                              <  2:380842/250‑1 (MQ=255)
              gAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGACAAATACAAAAAc                                                                                                                                                                                                                                           <  1:453652/251‑1 (MQ=255)
                         aCCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGACAAATACAAAAACTGGAGTT‑Gcc                                                                                                                                                                                                                                <  1:366697/250‑1 (MQ=255)
                               aCCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑ATTTTTCAGAcaaaaacaaaaaaaggaggtggccaga                                                                                                                                                                                                                             >  1:169681/1‑233 (MQ=255)
                                                  aaGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGACAAATACAAAAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACa                                                                                                                                                                                                      <  2:585919/251‑1 (MQ=255)
                                                  aaGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGACAAATACAAAAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACa                                                                                                                                                                                                      <  2:482810/251‑1 (MQ=255)
                                                    ggCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCCGAAAAATACAAAAAATGGAGTT‑GCCATCATGAgggacaggaaacaacaaca                                                                                                                                                                                                      >  2:321290/1‑231 (MQ=255)
                                                        aGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCC‑GAACAATATAAAAACAGGAATT‑TCCATAAAGAGCGAAAGtagtaattaacatgc                                                                                                                                                                                                   >  1:392975/1‑239 (MQ=255)
                                                                      gTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGACAAATACAAAAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGA‑Can                                                                                                                                                                                 <  2:269478/251‑2 (MQ=255)
                                                                               ccGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTGTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGACAAATACAAAAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGA‑CAGAGAT‑TGTCg                                                                                                                                                                       <  2:589041/251‑1 (MQ=255)
                                                                                    gTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCAGAAAAAAACAAAAAATGGGGTT‑GCCATGATGAGCGAAAATAATAAGCAACAGGCAGTGAAAAAATTGA‑CAAAGAA‑TGTCGCTaa                                                                                                                                                                   >  1:163318/1‑250 (MQ=255)
                                                                                          aTAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCCGACAAATACAAAAACTGGAGGT‑GCCATGATGAGCGAAAg                                                                                                                                                                                                                  >  1:475878/1‑199 (MQ=255)
                                                                                          aTAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGACAAATACAAAAACTGGAGTT‑GCCATGATGAGCGAAAg                                                                                                                                                                                                                  <  2:475878/199‑1 (MQ=255)
                                                                                                    tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCAGACAAATACAAAAACTGGAGTT‑GCCATGATGAGCGAAAGAAATAAACAAAAAGCAGTGAATAAGTTGA‑AAGAGAT‑TGTCGCTAACTTTACCGCCATGAttt                                                                                                                                                  >  1:187766/1‑251 (MQ=255)
                                                                                                           cGCAAAACATTGCCGTTCCCTGCTTTGGCGCAATATTCATTCTTTTTTTCGCGTTGATTCAAGATCTGTTAATTACATATTCCCGGTGGAAAAAAAAAAAAACCCACATTTTTAGTGTTAGTTTGTGGGGGTGAT‑TTTTCCCAGACAAATACAAAAACTGGAGTT‑GCCATGATGAGGTAAATTAATAAGAAACGGGCGGGAAATAGGTTGA‑CAGAGAT‑TGTGCCTAACTTTTCCGCCATGATTTCTA‑CCCg                                                                                                                                          <  2:157899/251‑1 (MQ=255)
                                                                                                            gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCCGACAAAAACAAAAAAAGGAGTTTGCC‑TGATTAGCGGAAGGAAAAAGCAAAAAGCAGTGGATAAATTTA‑AAGAGAT‑TGTTGCTAACTTTACCGCCATGGttttttcccc                                                                                                                                           >  1:340521/1‑243 (MQ=255)
                                                                                                                aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑ATTTTTCAGAAAAATACAAAAAATGGAGTT‑GCCATGATGAGCGAAAGAAATAAACAACAAGCAGGGAATAAATTGA‑CAGAGAA‑TGTTGCTAACTTTACCCCCCTGATTTTTT‑CCCCAATGc                                                                                                                                     >  1:583295/1‑251 (MQ=255)
                                                                                                                          ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCC‑GAAAAAAAAAAAAAATGGGGTT‑GCCCTGATGAGGGAAAGAAATAAAAAAAAGGCAGTGAATAAAATGG‑CAGAGAT‑TGTCGCTAAATTTAACGCCATGAATTTTA‑CCCGAATGCCtgatggcgg                                                                                                                           >  1:159448/1‑247 (MQ=255)
                                                                                                                                       cacAATATTCATTCTTATTTTCGCTTTGATCCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTGAA‑TTTTTCCAGAAAAATAAAAAAACGGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGA‑CAGAGTT‑TGTCGCTATCTTTACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTa                                                                                                              <  2:583295/249‑1 (MQ=255)
                                                                                                                                        gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑ATTTTTCACACAAATAAAAAAAAAAGAGGT‑GGCATGAATAGCGAAAGGAATAAACAAAAAAAAGCGAATAAAATTA‑CAGAGATATG‑CGCTAAATTTAACGTCATGAATTCTA‑TCCGAATGCCTGATGACGTGGGGGAGaaaatt                                                                                                              >  2:434097/1‑247 (MQ=255)
                                                                                                                                              ttcattcTTATTTTCGCGTTGATTCACTAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAAATTTTTAGTGGTATTTTGTGGCGGTGAA‑TTTTTCAAGACAAATAAAAAAACGGGAGTT‑GCCATGATGAGCAAAAGTAATAAGCAACGGGCAGTGAATAATTGGA‑CAGAGAT‑GGTCGCTAATTTTACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAg                                                                                                        <  2:187766/250‑1 (MQ=255)
                                                                                                                                                    cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑ATTTTTTAGACAAATACAAAAAATGGAGGT‑GCCATGATGAGCGGAAGAAATAAACAAAAGGCAGGGAATAAATTGA‑CAGAGAA‑TTTCGCTAACTTTAACGCCATGGTTTTTA‑CCCGAATGGCTTATGACGTGGGGGGTAAACTAAAACAGCTCAAgg                                                                                                 >  1:127192/1‑251 (MQ=255)
                                                                                                                                                     ttattttcgattgtaTTCACGAATTTTTAATGACAATTTCAGGGTGGCAAAAAATATAACACAACTTTTTTAGGGGTATTTGGTGGCGGTGAA‑TTTTTCAAGAAAAATACAAAACCTGGGTTT‑GCCATGATGAGCAAAAGTAATAAGCAACAGGAGGTGAATAAGTTGA‑AAGATAT‑TGTCGCTAATTTTGCCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAgg                                                                                                 <  2:390657/236‑1 (MQ=255)
                                                                                                                                                                               ttAATTACATATTCACGGTGGCAAAAAATATAAAACCATATTTTTAGTGGTAGTTTGGGGCGGTGAA‑TTTTTCCAGACAAATAAAAAACCTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGA‑CAGAGAT‑TGCCGCTAACTTTACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGaaa                                                                       <  1:331980/250‑1 (MQ=255)
                                                                                                                                                                                tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGAGGTAGTTTGTGGCGGTGAA‑TTTTTTCACACAAAAAAAAAAACTCAGGTT‑TGCATGGTGCGGGGAAGCAATAAACAACAGGACGTGAATAAAATGA‑CAGAGAA‑TGTCGCTAACTTTACCGCCCTGATTTTTTTCCCCAA‑GCCTGCTGGCGGGGGGGATAAACTAAAAAAGATAAAGGATGCCGAAAccttgatgaagggggaaa                                                                      >  1:300461/1‑233 (MQ=255)
                                                                                                                                                                                               ggTGGAAAAAAAAATAAAACCAGATTTTTGTTGGTTGTTTTTGGCGGTGAT‑TTTTTCCAGAAAAATCCAAAAACGGGAGTT‑GCCATTAGGAGCAAAATTAAAAAGCAACAGGCAGT‑AAAAAATTGAACGGAGAT‑TGGCGCTAACTTTACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATTGGGAAAATTATCTACCATACGAt                                                      <  2:257924/251‑1 (MQ=255)
                                                                                                                                                                                                         aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCCCACAAAAAAAAAAAATGGAGAT‑GCCACTATGAGCGGAACCAATAAACAACAAGGAGTGAATAAATTGA‑CAGAGAA‑TGGTGGAAACTTTTCTGCCCTGATTTTTA‑CACGAAAGCCTGATGACGTGGTGGGTAAAATAAAAAAGCTAAAGGATGCCGAAACGGAGTCGGCGGGGaaaaattattaacacacgaagttcgcaaa                                             >  1:53228/1‑225 (MQ=255)
                                                                                                                                                                                                                  ccACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCAAGACAAATACAAAAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGA‑CAGAGAT‑TGTCGCTAACTTTACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCaaaaa                                   <  1:321290/251‑1 (MQ=255)
                                                                                                                                                                                                                                               gAA‑TTTTTCCAGACAAATACAAAAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGA‑CAGAGAT‑TGTCGCTAACTTTACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTc       >  2:241737/1‑250 (MQ=255)
                                                                                                                                                                                                                                                aa‑TTTTTCCAAACAAATACAAAAACGGGATTT‑GCCATGATGAGCAAAAGTAATAAGAACCAGGCAGTAAATAAGTTGA‑CAAAGAT‑GGTCGCTAACTTTACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAg     <  2:340521/251‑1 (MQ=255)
                                                                                                                                                                                                                                                     tttCCAAACAAAAACAAAAACTGGAGTT‑GCCATGATGAGCGAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGA‑CAGAGAT‑TGTCGCTAACTTTACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGac  <  1:483505/250‑1 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                          
AAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGACAAATACAAAAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGA‑CAGAGAT‑TGTCGCTAACTTTACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGAC  >  NC_000913/3206188‑3206681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑