| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,001,911 | 0 | G | A | 61.5% | ‑4.1 / 10.2 | 13 | intergenic (‑122/+1) | fliA/fliX | RNA polymerase sigma factor FliA/small RNA FliX |
| Reads supporting (aligned to +/- strand): ref base G (5/0); new base A (0/8); total (5/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.77e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCC‑AGG‑CAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGG > NC_000913/2001687‑2002149 | gtgaagccggcggccgcATTGCTTGGGGACGCACCAGGGGGACATAACGCTGCCACAGCGGGTGTTTATCCATTACCCCTTCAGGGATATAGAGTGAATTCCAGATAAACAGCCCTGCGTTATATGAGTTATCGGAATAATTTTCCTTTTCTGCAGGTTTTTTAACGGGACGATTGGGGGGTGAAAGGAGGGGTTATTGGGGGGTTACGGGTAAATACC‑AGG‑CAAAAAAAACCCCCGCCGGTGGCGGGGaa < 1:555889/237‑1 (MQ=255) ggaccccccAGCGGGCAATAACGCTGCCACAGCGAGTGTTTATCAATTCCACCTTGAGCTGTATAGAGTAAATTCGCAATAAACAGCCCTGCTTTATATGATTTTTCGGCATGATTTCCCTTTTCTGCGGGGTTTTTAATCGGACGATTAGTGGGTAAAATGAGGGGTTATTTGGGGTTTACAGGAAAATCCC‑AGG‑CAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCttt < 1:43567/243‑1 (MQ=255) agggtaatttcccGATAAACGCCCCTTCTTTATATGGTTTTTCGGCAGGTTTTTCCGTTTCTGCAGGTTTTTTAATCGAAGAATTGGTGGGTAAAATGGTGGGTTTTTTGGGGGTTA‑AGGTTAATTCCCAGG‑AAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCTGCCTTCAAAAATGTGCAAATTCAATAAATTGc < 1:211499/239‑1 (MQ=255) ccccTGCGTTATATGAGTTTTCGGCATGATTATCCGTTTCTGCAGGTTTTTTAATCGGACGATTAGGGGGTAAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCA‑AGG‑CAAAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGAt < 1:316492/250‑1 (MQ=255) tCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGAGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCC‑AGG‑CAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCa > 1:228651/1‑250 (MQ=255) gttttttAAGCGGACAATGAGTGGAGGAAATGAGGGTTTGTTGGGGGGTTA‑AGGTTAACTCC‑CGGACAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGtt < 2:119131/249‑1 (MQ=25) gATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCC‑AGG‑CAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACg > 1:56079/1‑251 (MQ=255) gggTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCC‑AGG‑CAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAAcc > 2:300192/1‑251 (MQ=255) aagggggggTTTTTGGGGGGTAACAGGTAAATCCC‑AGG‑AAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTgcag < 1:165353/246‑1 (MQ=38) aTTTGGGGGTTACAGGTAAATTCC‑AGG‑CAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGa > 2:304433/1‑250 (MQ=255) gggTTACAGGTAAATGCA‑AGG‑CAAAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACctgct < 1:369418/251‑1 (MQ=255) gaaaaTCCC‑AGG‑CAAAAAAACCCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCt < 1:389609/249‑1 (MQ=255) aaaTTCC‑AGG‑CAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTgg > 2:115194/1‑251 (MQ=255) | GTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCC‑AGG‑CAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGG > NC_000913/2001687‑2002149 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |