Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,603,7120AT24.0% 116.3 / 12.3 51I232F (ATC→TTC) lsrCAutoinducer‑2 ABC transporter membrane subunit LsrC
Reads supporting (aligned to +/- strand):  ref base A (9/29);  new base T (11/1);  total (21/30)
Fisher's exact test for biased strand distribution p-value = 4.25e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACAGCTCTCCGCCCCGCTGCTGCTTGGCGTTTCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGTTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTATCCCCAACCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGCAG‑CCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGG‑TTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGT‑ACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCT  >  NC_000913/1603465‑1603962
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                
aCAGCTCTCCGCCCCGCTGCTGCTTGGCGTTTCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGTTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTATcc                                                                                                                                                                                                                                                               <  1:527100/251‑1 (MQ=255)
             ccGCTGCTGCTTGGCGTTTCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGTTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGGTTGTTTCGC‑AGATT‑GGGTTTTTTCCCCA‑CCC‑GACCgg                                                                                                                                                                                                                                                 >  1:98853/1‑251 (MQ=255)
               gctgctgctTGGCGTTTCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGTTTGGACGCAGGTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTTGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTATCCCCAACCA‑GACCGG‑Ta                                                                                                                                                                                                                                              <  2:344978/251‑1 (MQ=255)
                    tgctTGGCGTTTCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGTTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGGATTGGGTTTGCTTCGC‑AGCTT‑GG‑GTTTATCCCCAACCA‑AACCGGGTACCgg                                                                                                                                                                                                                                          >  2:109002/1‑251 (MQ=255)
                         ttcttttCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTTGCAAAGACGGCGTTTGGACGCAGTTTTTATGCCACGGGCGATCCTTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGGACTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTTTCCCCCAccccaacggtacccggccggg                                                                                                                                                                                                                                      >  1:322717/5‑232 (MQ=255)
                                 aGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGTTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTATCCCCAACCA‑GACCGG‑TACCGGGCTGGAGATGAAAg                                                                                                                                                                                                                            <  1:293194/251‑1 (MQ=255)
                                         gttggttgACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGTTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGGATTGTGGTTTCTTCGC‑AGATT‑GG‑GTTTTTCCCCCACCA‑GAACGG‑GACCGGGCTGGAGAAGAAAGCAA‑TTGc                                                                                                                                                                                                                    >  1:321456/1‑250 (MQ=255)
                                                gACGAGAATTCTGGTGGCATTTATGGCCTGGCTGCTGGTAAAGACGGCGTTTGGACGCAGTTTTGATTCCACGTGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGC‑AAATT‑GG‑TTTTATCCCCACCCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGctg‑cctgc                                                                                                                                                                                                            <  2:144099/250‑1 (MQ=255)
                                                   gATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGTTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGGAATGTGTTTGGTTTGC‑AGATT‑GG‑TTTTTTTCCCCACCA‑GACCGG‑TACCGGGCTGGGGATGAAAGCAA‑ATGCAG‑CCcgcgggg                                                                                                                                                                                                        >  1:52408/1‑248 (MQ=255)
                                                             gtggCATTTATGGCCTGGCTGCTGGCAAAGACGGCGTTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGGATTGTGTTTTCTTTGCCGGTTT‑GG‑TTTTTTCCCCACACA‑‑ACCCG‑GACCGGGCTGGGGATGAAAACAA‑TTGTAG‑CCTGCGGGCTGggcggg                                                                                                                                                                                                >  2:38716/1‑248 (MQ=255)
                                                                        ggCCTGGCTGCTGGCAAAGACGGCGTTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTATCCCCAACCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGCAG‑CCTGCGTGCTGGGCGGCATTAGTTTGCTc                                                                                                                                                                                    <  2:221788/250‑1 (MQ=255)
                                                                        ggCCTGGCTGCTGGCAAAGACGGCGTTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTATCCCCAACCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGCAG‑CCTGCGTGCTGGGCGGCATTAGTTTGCTCg                                                                                                                                                                                   <  1:468345/251‑1 (MQ=255)
                                                                              gctgctGGCAAAGACGGCGTTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCCTTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGGATTGTTGTTGCTTCCC‑AGATTTGG‑TTTATTCCCCCCCCA‑‑CCCGG‑GACCGGGCTGGGGATGAAAGCAAATTGC‑G‑ACTGCGTGGTGGGGGGCGTTAGGTTTCTCggggggt                                                                                                                                                                             >  1:563734/1‑246 (MQ=255)
                                                                                 gctgGCAAAGACGGCGTTTGGGCGCAGTTTTTATGCCACGGGAGATACTTTACAGGGCGCTAGTCCACTGGGCGTTCGTACTGACGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCCCTGGCGGGAATTGTGGTTGCTTCGT‑AGATT‑GG‑TTTTATCCCCACCCA‑GACCGG‑TACCGGGTTGGAGATGAAAGCAA‑TTGCAG‑CCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGG‑TTc                                                                                                                                                                           <  2:375147/249‑1 (MQ=255)
                                                                                 gctgGCAAAGACGGCGTTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTATCCCCAACCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGCAG‑CCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGG‑TTCCg                                                                                                                                                                         <  1:325101/251‑1 (MQ=255)
                                                                                             ggCGTTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGGATTGTGTTTTCTTCGC‑AGATT‑GG‑GTTTTTTCCCACCCA‑GACCGG‑TACCGGGCTGGGGGTGGAAGCAA‑TTTCAG‑CCTGCGGGCTGGGGGGCGTTTGTTTTCTCGGTGG‑TTTCGGGGCggtagatg                                                                                                                                                             >  1:134144/1‑244 (MQ=255)
                                                                                             ggCGTTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGGATTGTGTTTGCTTCGC‑AGATT‑GG‑GTTTATTCCCAACCA‑GACCGG‑TACCCGGCTGGGGAAGAAAACAA‑TTGCAG‑CCCGCGGGCTGGGGGGCATTTGTTTTACCGGTGG‑TTCCGGGGCggtccttg                                                                                                                                                             >  1:274020/1‑244 (MQ=255)
                                                                                             ggCGTTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTATCCCCAACCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGCAG‑CCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGG‑TTCCGGTGCGAt                                                                                                                                                                  <  2:134144/246‑1 (MQ=255)
                                                                                                 tttGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGGATTGTGTTTGCTTCGC‑AGATT‑GG‑GTTTATCCCCAACCA‑GACCGG‑TACCCGGCTGGAGGTGGAAGCAA‑TTGCAG‑CCCGCGGGCCGGGGGGGCTTAGTTTGCTCGGTGG‑GTTCGGTGCGATCATTggggg                                                                                                                                                         >  1:294537/1‑248 (MQ=255)
                                                                                                    ggACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGGATTGTGTTTGCTTCGC‑CGATT‑GG‑TTTTTTCCCCCACCA‑GACCGG‑TAc                                                                                                                                                                                                                                             >  1:117491/1‑167 (MQ=255)
                                                                                                    ggACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTATCCCCAACCA‑GACCGG‑TAc                                                                                                                                                                                                                                             <  2:117491/167‑1 (MQ=255)
                                                                                                      aCGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAAATGTGTTTTCTTCGC‑AGATTTGG‑TTTT‑TCCCCAAACA‑GACCCG‑GACCGGGCTGGGGATGTAAGCAA‑ATGCAG‑CCCGCGGGCTGGGGGGCATTAGTTTTCTCGGTGG‑GTCCGGTGCGGTCCTTTGTGCgggac                                                                                                                                                    >  2:229406/1‑248 (MQ=255)
                                                                                                       cGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGGATTGGGGTTGCTTCGC‑CGATT‑GG‑TTTTTTCCCCAACCA‑GCCCGG‑GACCGGGCTGGGGATGAAAGCAA‑ATGCAA‑CCTGCGTGCTGGGCGGCATTAGTTTGCTCGGGGG‑TTCCGGGGCGATCATTGGTGCGGGACt                                                                                                                                                   >  1:543547/1‑251 (MQ=255)
                                                                                                       cGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTATCCCCAACCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGCAG‑CCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGG‑TTCCGGTGCGATCATTGGTGCGGTACt                                                                                                                                                   <  1:114878/251‑1 (MQ=255)
                                                                                                         cAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTATCCCCAACCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGCAG‑CCTGc                                                                                                                                                                                                            <  1:201560/193‑1 (MQ=255)
                                                                                                                  tGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAGTTGTGTTTTCTTCTC‑AGATT‑GGGTTTTTTCCCCA‑CCA‑CAACGG‑TTCCGGGGTGGGGATGGAAGCAA‑TTTCAG‑CCCGCGTGCGGGGCGGCCTTTGTTTTCTCGGCGG‑TTCCGGGGCGATcgattgtggggggatcggctcttggt                                                                                                                                        >  2:410380/1‑226 (MQ=255)
                                                                                                                   gCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTATCCCCAACCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGCAG‑CCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGG‑TTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGtt                                                                                                                                       <  2:45933/251‑1 (MQ=255)
                                                                                                                        gggCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTATCCCCAACCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGCAG‑CCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGG‑TTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGa                                                                                                                                  <  2:52408/251‑1 (MQ=255)
                                                                                                                                         ggtcttgttaaCTGGGCGATCGTACTGAATCCATTCGTATTGTGTAATTTTCGTTGAACGGTTTCATGGCGGCACTGGCGAGAATTGTGTTTGCGTCGC‑AGATT‑GG‑TTTTATCCCCAACCA‑GACCGG‑TTCCGGGCTGGAGATGAAAGTAA‑TTGCAG‑CCTTCGTGCTGGGCGGCATTAGTTTGCTCGGTGG‑TTCCGGTGCGATCATTGGTGCGGTACTCGGCGAATGGTTACTGACGCAGATCGATAGCGT‑a                                                                                                                <  2:321127/242‑1 (MQ=255)
                                                                                                                                           gcTCGTCAACTGGGCGTTCGTATTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTATCCCCAACCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGCAG‑CCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGG‑TTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGAt                                                                                                                       <  2:114002/243‑1 (MQ=255)
                                                                                                                                           gcTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTTGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTATCCCCAACCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGCAG‑CCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGG‑TTCCGGTGCGATCATTGGTGCGATACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGT‑ACt                                                                                                              <  2:340628/251‑1 (MQ=255)
                                                                                                                                           gcTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTATCCCCAACCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGCAG‑CCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGG‑TTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGT‑ACt                                                                                                              <  2:98853/251‑1 (MQ=255)
                                                                                                                                           gcTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTATCCCCAACCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGCAG‑CCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGG‑TTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGACCGATAGCGT‑ACt                                                                                                              <  1:408295/251‑1 (MQ=255)
                                                                                                                                                          gTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTATCCCCCACCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGAAG‑ACCGCGTGCTGGGGGGGCTTAGTTTGCTCGGGGG‑GTCCGGGGCGAACATTGGTGCGGGACTCAGCGCACTGTTCCTGGCGCAGATCGATAGCGT‑ACTGGGGCTGtttgggat                                                                                               >  1:445358/1‑245 (MQ=255)
                                                                                                                                                                    aaGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTATCCCCAACCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑tt                                                                                                                                                                                                                      <  1:582619/125‑1 (MQ=255)
                                                                                                                                                                          ttCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGGATTGGGTTTGCTTTGC‑CGATT‑GG‑GTTTATCCCCCACCA‑GACCCG‑GACCGGGGTGGAGATGAAAGCAA‑TTGCAGGCCT‑CGTGCTGGGCGGGATTAGTTTGCTCGGGGGGTTCCG‑TGCGATCATTGGGGCGGTACTCGGGGCCTGGTTCCTGACGCAGATCGATAGCGTGATTGGT‑CGGTTGCGCGTTCCGGCCtggggggat                                                                               >  2:168671/1‑245 (MQ=255)
                                                                                                                                                                              cATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTATCCCCAACCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGCAG‑CCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGG‑TTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATgg                                                                                                                                         <  1:66988/190‑1 (MQ=255)
                                                                                                                                                                                ttGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTTTGTTTGGTTCGC‑AGAGT‑GG‑GTTTTTCCCCAACCACAACCGG‑‑ACCGGGGTGGGGAGGAAAAGAA‑ATGCAG‑ACTGCGGGCTGGGCGGGATTAGGTTGCTCGGGGG‑GTCCGGGGTGAGCCTTGGGGCGGGACTCGGCGCATGGGTCCTGTAGCAAATCGATAGCGT‑ACTGGGGCTGGTgcgcctttcgggctggggggatgagttt                                                                         >  1:397564/1‑227 (MQ=255)
                                                                                                                                                                                    ggCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTATCCCCAACCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGCAG‑CCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGG‑TTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGT‑ACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATcg                                                                     <  2:321456/251‑1 (MQ=255)
                                                                                                                                                                                     gCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTATCCCCAACCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGCAG‑CCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGG‑TTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGT‑ACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATcgc                                                                    <  1:229406/251‑1 (MQ=255)
                                                                                                                                                                                         tttCGTTGAACGGCTGCATGGCGGCACTGGCGGGGATTGTGTTTGCTTCGC‑AGATT‑GG‑GTTTATCCCCAAACA‑GACCGG‑TACCGGGGTGGAGGTGGAAGCAA‑TTTCAG‑CCTGCGTGCTGGGCGGGCTTAGTTTGCTCGGGGG‑GTTCGGTGCGATCCTTGGTGCGGGACTTGGCGCATGGTTCCTGAAGCAGATCGATAGCCG‑ACTGGGGCGGTTGCGCATTCCGGCATGGGGGAATGATTTTATTGCgggg                                                                >  1:503638/1‑250 (MQ=255)
                                                                                                                                                                                                gAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTATCCCCAACCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGCAG‑CCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGG‑TTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGT‑ACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTc                                                         <  1:38716/251‑1 (MQ=255)
                                                                                                                                                                                                   cGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTATCCCCAACCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGCAG‑CCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGG‑TTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGT‑ACTGGTGCTGTTGCGCATTCCGGCAt                                                                                       <  2:405888/218‑1 (MQ=255)
                                                                                                                                                                                                            ggcggcACTGGCGGGAATTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTATCCCCAACCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGCAG‑CCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGG‑TTCCGGTGCGATCATTGGTGc                                                                                                                                                         <  1:182088/144‑1 (MQ=255)
                                                                                                                                                                                                                    tGGCGGGAATTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTATCCCCAACCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGCAG‑CCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGG‑TTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGT‑ACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGgtggtt                                     >  1:271188/1‑248 (MQ=255)
                                                                                                                                                                                                                      gCGGGAATTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTATCCCCAACCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGCAG‑CCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGG‑TTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGT‑ACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGa                                   <  1:150606/251‑1 (MQ=255)
                                                                                                                                                                                                                       cGGGAAGTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTTTCCCCAACCA‑GTCCGG‑TACCGGGCTTGAGATGAAAGCAA‑TTGCAG‑CTGGCGTGCTGGGCGGCATTAGTTTGCTCGGTGG‑TTCAGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGT‑ACTGGTGGTGTTGCGCATTCCGGGATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGAt                                  <  2:563734/251‑1 (MQ=255)
                                                                                                                                                                                                                                         cGC‑AGATT‑GG‑TTTTATCCCCAACCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGCAG‑CCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGG‑TTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGT‑ACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTgc                 <  1:101733/250‑1 (MQ=255)
                                                                                                                                                                                                                                             ngATT‑GG‑TTTTATGCCCAACAA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGAAG‑CCTGCGTGCTGGGTGGCATTAGTTTGCTCGGTGG‑TTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGT‑ACTGGGGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGAGCTGGAGTTTTATgagatcccgggttatgtggtg             >  2:277877/2‑231 (MQ=255)
                                                                                                                                                                                                                                                   gg‑TTTTATCCCCAACCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGCAG‑CCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGG‑TTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGT‑ACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAAc         <  2:294537/250‑1 (MQ=255)
                                                                                                                                                                                                                                                   gg‑TTTTATCCCCAACCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGCAG‑CCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGG‑TTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGT‑ACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGGGTTTGATGGGCGCCTGCGTTGTGCGCTGGAACg        >  1:337441/1‑251 (MQ=255)
                                                                                                                                                                                                                                                          aTCCCCAACCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGCAG‑CCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGG‑TTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGT‑ACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGGTGTGCGCTGGGACGTAATCt  >  1:81389/1‑251 (MQ=255)
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                
ACAGCTCTCCGCCCCGCTGCTGCTTGGCGTTTCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGTTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGC‑AGATT‑GG‑TTTTATCCCCAACCA‑GACCGG‑TACCGGGCTGGAGATGAAAGCAA‑TTGCAG‑CCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGG‑TTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGT‑ACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCT  >  NC_000913/1603465‑1603962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑