Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913803,8690GT36.4% 42.6 / 11.0 33V123L (GTG→TTG) ybhJputative hydratase YbhJ
Reads supporting (aligned to +/- strand):  ref base G (5/16);  new base T (12/0);  total (17/16)
Fisher's exact test for biased strand distribution p-value = 2.26e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAACTTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCAT‑CAATATATGCG‑TGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACA‑CCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACACCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTG‑ACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGC  >  NC_000913/803623‑804115
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                          
aTTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTtgtg                                                                                                                                                                                                                                                          <  1:516833/249‑1 (MQ=255)
          cGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCatat                                                                                                                                                                                                                                               <  1:78207/250‑1 (MQ=255)
                     tCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGGTTTTTTTGTCTCCCCATCTTGTGGtcctccc                                                                                                                                                                                                                                   >  2:496716/1‑250 (MQ=255)
                       cGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTcatccat‑c                                                                                                                                                                                                                                <  1:256855/251‑1 (MQ=255)
                         gAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGAGTTTTTGTGCCttcccccattgcgggttttcatca                                                                                                                                                                                                                                >  1:104132/1‑227 (MQ=255)
                          aaaTATGGTAAAAATTAAAATTAAGTTTGAATCATTAGCCTCTCTCGATGTTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATGAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCAT‑CAat                                                                                                                                                                                                                             <  2:544806/251‑1 (MQ=255)
                                        ttAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCTTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTcatcc                                                                                                                                                                                                                                    <  2:92082/231‑1 (MQ=255)
                                        ttAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGGCTCCCCATATTGCGGGcatcccggtcgggagaacacaagac                                                                                                                                                                                                                >  1:92082/1‑231 (MQ=255)
                                                     tGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCAT‑CAATATATGCG‑TGAGATGATGGCAggcggc                                                                                                                                                                                                  <  1:477579/250‑1 (MQ=255)
                                                     tGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTTCCTTCACATATTGCGGGCATCCAT‑CCACATATGCGGTG‑GATGATggacggcgggg                                                                                                                                                                                                 >  1:323392/1‑242 (MQ=255)
                                                     tGATTCATTAGCCTCTCACGATATTACCGTTGTTGGTATTGTACAGTCCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCAT‑CAATATATGCG‑TGAGATGATGGCAggcggc                                                                                                                                                                                                  <  2:153836/250‑1 (MQ=255)
                                                                   ctcccGATATTTCCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCAT‑CAATATATGCG‑TGAGATGATGGCAGGCGGCGGCAAAATGATcc                                                                                                                                                                                     <  2:333847/245‑1 (MQ=255)
                                                                           atTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTTTGCCTCCGCCTATTGCGGGTATACAT‑CAATATATTCG‑TGAGATGATGGCAAGGGGCGGGAAAAAGATCCTCGGGTCAgc                                                                                                                                                                           >  1:249741/1‑250 (MQ=255)
                                                                           atTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCAT‑CAATATATGCG‑TGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAg                                                                                                                                                                            <  2:545983/250‑1 (MQ=255)
                                                                            ttACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTTTGCCTCCGCCTATTTCGGTCCTCCAA‑TAATATATGCG‑TGTGATGATGGCAGGGGGCGGGCAAATGTTCCTCGGGGCAGa                                                                                                                                                                           >  1:365451/1‑250 (MQ=255)
                                                                                        gTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTGCCTCCGCATATTTCGGGCATCCAT‑CAATATATGGG‑GGAGGTGATGGCAGGGGGGGGGAAAAAGATCCCCGGGGCAGACAGCCACA‑cccct                                                                                                                                                             >  1:62038/1‑249 (MQ=255)
                                                                                                   aCCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCAT‑CAATATATGCG‑TGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACA‑CCCGTTACGGTGCAtt                                                                                                                                                  <  1:300013/251‑1 (MQ=255)
                                                                                                                  ggTATGGACCTTTTCCCGCTGGCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCAT‑CAATATATGCG‑TGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACA‑CCCGTTACGGTGCATTAGGGACAATGGCAGt                                                                                                                                   <  2:187589/251‑1 (MQ=255)
                                                                                                                         aaCGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGGGCCTCCGCATTTTGCGGTCATCCATTCAA‑ATATTCG‑TGAGATGGAGGCAGGCGGCGGGAAAATGATCCTCGGGTCAGACAACCACA‑CCCGGTACGGTGGGTTAGGGaaaaagggaggcggtg                                                                                                                              >  1:193370/1‑234 (MQ=255)
                                                                                                                                cccGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCAT‑CAATATATGCG‑TGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACA‑CCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGgcggcg                                                                                                                     <  2:128424/251‑1 (MQ=255)
                                                                                                                                         gTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCAT‑CAATATATGCG‑TGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACA‑CCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTgg                                                                                                            <  1:128453/251‑1 (MQ=255)
                                                                                                                                               gCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTTTGCCTCCGCCTATTTCGGTCATCCAT‑CAATATATGCG‑TGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGGCAGACAGCCCCA‑CCCGTTACGGTGCATTTGGGGCAATGGCAGTCGGGGGGGGCGGCGGGGAGTTGGGaaaa                                                                                                       >  1:440130/1‑250 (MQ=255)
                                                                                                                                                     cAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTTTGGCTCCGCATATTTCGGTCCTCCAA‑CAAAATATGTG‑TGAGATGATGGCAGGGGGGGGGCAAATGATTCTCGGGTCAGAAAACCACAACCCGT‑ACGGGGCATTAAGGGCAATGGCAGGCGGCGAGGGCGGCGGGGGGTTGGGaaaaaagatgc                                                                                                >  1:169667/1‑244 (MQ=255)
                                                                                                                                                                      tctGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTTTGGCTCCGCATATTGCGGTCATCCAT‑CCAAATATGCG‑TGAGATGATGGGCGGGGGGGGCCAAATGATCCCCCGGTCAGACAGCCCCA‑CCCGGTACGGGGGCTTAGGGACAATTGCAGTCGGTGGGGGGGGCGGGGGGTTTGGAAAAAAGCTGGTTAATGAAACCTggggt                                                                               >  1:121349/1‑249 (MQ=255)
                                                                                                                                                                       ctGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTTTGCCTCCGCATATTGCGGTCATCCAT‑CAATATATGCG‑TGAGATGATGGCAGGCGGCGGCAAAAAGATCCTCGGGGCAGACAGCCACA‑CCCCTTACGGGGCATTAGGGACAAAGGCAGTCGGGGGGGGGGGCGGGGAGGTGGTAAAACAGCCGCTTTATGACACCTggggt                                                                               >  1:513719/1‑248 (MQ=255)
                                                                                                                                                                                   cggcACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTTTGGCTTCTCCTATTCCGGGCATCCCT‑CAATATATTCG‑GGAGATGATGGCACGATGTGGCCAAATGATCCTCCGGTCAGACAGCCACA‑CCCGTTACGGTGCGTTAAGGGCAAATGCAGTCGGATAGGGGGGCGGTGAGTTGGTAAAACCACTGCGTAATTACCACTGGGGTATGGGCTATCCgg                                                                  >  1:397943/1‑251 (MQ=255)
                                                                                                                                                                                      cACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCAAGCGTTATGGCGGGCTTTTTTTGCCTCCCCATTTTGCCGGCATCCCT‑TAATATATGCG‑TGAGATGAAGGAAGGCGGCGGCCAAATGAACCTCCGGTAAAAAAGCCACA‑CCCGTTACGGGGTATTAGGGGCAATGGCAGGCGGGGAGGGCGGGCGTGAGGTGGTAAAACAGGCGCTTAAAAAAACCCGGGGAATCGACTATCCggggg                                                               >  1:207912/1‑249 (MQ=255)
                                                                                                                                                                                                      gaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCAT‑CAATATATGCG‑TGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACA‑CCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACACCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCt                                               <  1:163661/251‑1 (MQ=255)
                                                                                                                                                                                                            gTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCAT‑CAATATATGCG‑TGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACA‑CCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACACCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTG‑ACCgg                                        <  1:489947/251‑1 (MQ=255)
                                                                                                                                                                                                                          ggcggcCCAGCGTTATGGCGGTATTTTTTTGCCTCCGCACATTGCGGTCATCCAT‑CAATATATGCG‑TGAGATGATGGGAGGCGGCGGCAAAATGATCCTCCGGTCAAACAGCCACA‑CCCGTTTCGGGGGCTTTGGGACAATGGCAATCGGGGGGGGCGGGGGGGGGGTGGGTAAACAACTGCTTAATGAAACCCGGGGTATCGACCATCCCGGCGGGGGTGCGGGGCCTCTGCACGGGAAAACC‑GCGCCgg                          >  2:524743/1‑250 (MQ=255)
                                                                                                                                                                                                                           gcggcCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCAT‑CAATATATGCG‑TGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACA‑CCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACACCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTG‑ACCGGAAAACCTGCGCCGTa                         <  2:62038/251‑1 (MQ=255)
                                                                                                                                                                                                                                       tATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCAT‑CAATATATGCG‑TGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACA‑CCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACACCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTG‑ACCGGAAAACCTGCGCCGTATGTGGGGCcac              <  1:208933/250‑1 (MQ=255)
                                                                                                                                                                                                                                           gCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCAT‑CAATATATGCG‑TGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACA‑CCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACACCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTG‑ACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGAt         >  2:287041/1‑251 (MQ=255)
                                                                                                                                                                                                                                                  ttttGTGCCTCCGCATATTGCGGTCATCCAT‑CAATATATGCG‑TGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACA‑CCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACACCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTG‑ACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGgcgc  >  1:8461/1‑251 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                          
ATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAACTTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCAT‑CAATATATGCG‑TGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACA‑CCCGTTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACACCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTG‑ACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGC  >  NC_000913/803623‑804115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑