Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	499,060	T→G	D305A (GAC→GCC)	aes ←	acetylesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	499,060	0	T	G	100.0%	198.7 / NA	54	D305A (GAC→GCC)	aes	acetylesterase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (28/26);  total (28/26)

CGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATGCGCCGCTAACGTCTGGTAAAGCAGACGGCTGTCATCCAGCAGCGGATCGAACTCCGCCCCGGCAATAAAACAGGGCGGAACTTCGCGAGTGAGATCATTATTAAACAGACAGTAATACGGCGACTCGCGGTCCGCGTCGTTGCTTAAATATGCCTCTTCG  >  NC_000913/498816‑499302                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   cGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCggcggc                                                                                                                                                                                                                                                <  2:609324/249‑1 (MQ=255)  ggAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGGAGAGCCTcggcggcggg                                                                                                                                                                                                                                             >  1:487401/1‑250 (MQ=255)  ggAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGt                                                                                                                                                                                                                                             <  1:251797/251‑1 (MQ=255)             cGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTTCGGCGCTTAATGCCACGCCGGAACAAATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAACTGAGCGGGAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGGTTTCATCATcc                                                                                                                                                                                                                                  >  2:110350/1‑251 (MQ=255)                aGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTTTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAAATGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGGTTTCCTCATCCGTg                                                                                                                                                                                                                               >  1:558888/1‑251 (MQ=255)                    cATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagt                                                                                                                                                                                                                           <  1:551663‑M1/251‑5 (MQ=255)                         tCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCAGTGtagtcgta                                                                                                                                                                                                                       <  1:160322‑M1/250‑9 (MQ=255)                                         ggCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtg                                                                                                                                                                                                      <  2:163826‑M1/251‑26 (MQ=255)                                         ggCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCCCTAAAGCTGAGCGGTAAAGAAATGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGGTTTCATCATCCGTGtaggcggaggcatttgacatttttg                                                                                                                                                                                                      >  1:202033‑M1/1‑226 (MQ=255)                                               gctgcGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgat                                                                                                                                                                                                <  1:617708‑M1/251‑32 (MQ=255)                                                ctgcGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCCGCGCTTAATGCCACGCCGGAACATAACGAAAAGATGGCTAATCTTGTTGGCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGCCGCGAAGAGCCTCGGCGGCGGGTTTCATCATCCGTGtagtcgtaggcattagaaatttttgcctga                                                                                                                                                                                                 >  1:567262‑M1/1‑219 (MQ=255)                                                  gcGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatg                                                                                                                                                                                               <  2:389879‑M1/249‑33 (MQ=255)                                                    ggATTGTCTGGAGACGCTGGAAGAGAATGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGTCGTCGCGAAGAGCCTCGGCGGCGGTTGTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgtc                                                                                                                                                                                           <  2:564951‑M1/251‑37 (MQ=255)                                                        tGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgct                                                                                                                                                                                        <  1:352211‑M1/250‑40 (MQ=255)                                                              gagaCGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGGTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcg                                                                                                                                                                                 >  1:85047‑M1/1‑205 (MQ=255)                                                                           agaTTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATTATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAAAACTGAGCGCCGTCGCGAAAAGCCTCGGCGGCGGGTTTCATCATCCGTGtagtcgtaggcattagacatttgtggctgatgcgacgcttgacgcgtcttatcaggcct                                                                                                                                                                    >  1:406768‑M1/1‑192 (MQ=255)                                                                             aTTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAAATATGGCTTATCTTGTTGCCCCGTATCGCTAAAGCTGAGCGGGAAAGAACCGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCCTCCGGGtagtcggaagccttagacctttgttcccggtgcgacgcttgacgcgtcttatcaggcctac                                                                                                                                                                  >  1:126230‑M1/1‑190 (MQ=255)                                                                                  cGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAAGATGAATAGATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccg                                                                                                                                                             <  1:334594‑M1/251‑67 (MQ=255)                                                                                             cggagGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatcc                                                                                                                                                  <  2:318430‑M1/249‑78 (MQ=255)                                                                                                      tCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaatt                                                                                                                                           <  1:578171‑M1/249‑85 (MQ=255)                                                                                                       ccTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggt                                                                                                                                        <  2:303315‑M1/251‑88 (MQ=255)                                                                                                              agcgaaattgtggtgttctagggaattccggcgcttaatgccacgccggaACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaat                                                                                                                                 <  1:563627‑M1/227‑95 (MQ=255)                                                                                                                cggcggGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgc                                                                                                                               <  1:425791‑M1/251‑97 (MQ=255)                                                                                                                     ggAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTCATCTTGTTGCCGCGTATCACTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCCTCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgat                                                                                                                          >  1:591740‑M1/1‑150 (MQ=255)                                                                                                                       aaaaaaTATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgaagc                                                                                                                        >  2:16997‑M1/1‑148 (MQ=255)                                                                                                                          aaaTATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgat                                                                                                                     <  2:458504‑M1/251‑107 (MQ=255)                                                                                                                              atGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgctt                                                                                                                 <  2:348461‑M1/251‑111 (MQ=255)                                                                                                                                   tatatTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgc                                                                                                            >  1:610159‑M1/1‑136 (MQ=255)                                                                                                                                      atgccggcGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgactcttgacgcgtcttatcaggcctacatccggtgccgcatccggcaattggtgcacaattcctgatgcgatgcttgacgcatc                                                                                                         <  2:581479‑M1/248‑119 (MQ=255)                                                                                                                                      atTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatc                                                                                                         <  1:110350‑M1/251‑119 (MQ=255)                                                                                                                                         ccGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatctt                                                                                                       <  2:487401‑M1/250‑121 (MQ=255)                                                                                                                                           ggCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGGTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatc                                                                                                    >  2:58431‑M1/1‑128 (MQ=255)                                                                                                                                            gcgcTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtag                                                                                                                                                                                                                            <  1:74501‑M1/130‑4 (MQ=255)                                                                                                                                            gcgcTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtag                                                                                                                                                                                                                            >  2:74501‑M1/1‑127 (MQ=255)                                                                                                                                               cTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggc                                                                                                >  1:211883‑M1/1‑124 (MQ=255)                                                                                                                                                  aaTGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcct                                                                                              >  1:96749‑M1/1‑121 (MQ=255)                                                                                                                                                        aCGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgg                                                                                       <  2:558888‑M1/251‑137 (MQ=255)                                                                                                                                                         cGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatggg                                                                                      >  1:597222‑M1/1‑114 (MQ=255)                                                                                                                                                             ggAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtacc                                                                                  >  1:134295‑M1/1‑110 (MQ=255)                                                                                                                                                                       gAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggt                                                                          >  2:572234‑M1/1‑100 (MQ=255)                                                                                                                                                                         aaTGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggctcggtaggc                                                                      >  2:86565‑M1/1‑98 (MQ=255)                                                                                                                                                                            gatgGCTAATCTTGTTGCCGCGTATCTCTAAAGCTGCGAGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgctggacgcatcttatcaggcctacaaggggtaccggatcggtaggccgg                                                                   <  2:176463‑M1/251‑157 (MQ=255)                                                                                                                                                                             atgGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccgg                                                                   >  1:588974‑M1/1‑94 (MQ=255)                                                                                                                                                                                    aTCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaataggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcg                                                           >  1:127538‑M1/1‑87 (MQ=255)                                                                                                                                                                                        tgttgCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgttt                                                        <  1:462163‑M1/250‑168 (MQ=255)                                                                                                                                                                                         gttgCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttac                                                      <  1:343304‑M1/251‑170 (MQ=255)                                                                                                                                                                                           tgCCGCGCATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgc                                                    >  2:575770‑M1/1‑80 (MQ=255)                                                                                                                                                                                              cgcgTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgc                                                 >  2:4993‑M1/1‑77 (MQ=255)                                                                                                                                                                                                 gTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatc                                              >  1:180502‑M1/1‑74 (MQ=255)                                                                                                                                                                                                 gTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATAATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctaccaccggtgccgcatccggcaattggggcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggattaggcgtttccgccgcatc                                              >  2:427699‑M1/1‑74 (MQ=255)                                                                                                                                                                                                      gCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccggc                                          >  1:33302‑M1/1‑69 (MQ=255)                                                                                                                                                                                                                aGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccggcaagaatagagc                               >  1:138366‑M1/1‑59 (MQ=255)                                                                                                                                                                                                                          aaCTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccggcaagaatagagcaccagttaac                     >  1:418945‑M1/1‑49 (MQ=255)                                                                                                                                                                                                                              gAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccggcaagaatagagcaccagttaaccg                   <  2:102009‑M1/249‑205 (MQ=255)                                                                                                                                                                                                                                            agagCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccggcaagaatagagcaccagttaaccgaacttactctgcgccc   <  1:280535‑M1/251‑221 (MQ=255)                                                                                                                                                                                                                                              agCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccggcaagaatagagcaccagttaaccgaacttactctgcgccca  >  2:73234‑M1/1‑29 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   CGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATGCGCCGCTAACGTCTGGTAAAGCAGACGGCTGTCATCCAGCAGCGGATCGAACTCCGCCCCGGCAATAAAACAGGGCGGAACTTCGCGAGTGAGATCATTATTAAACAGACAGTAATACGGCGACTCGCGGTCCGCGTCGTTGCTTAAATATGCCTCTTCG  >  NC_000913/498816‑499302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑