Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,174,041	A→T	E320D (GAA→GAT)	birA →	DNA‑binding transcriptional repressor/biotin‑[acetyl‑CoA‑carboxylase] ligase BirA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,174,041	0	A	T	100.0%	170.1 / NA	43	E320D (GAA→GAT)	birA	DNA‑binding transcriptional repressor/biotin‑[acetyl‑CoA‑carboxylase] ligase BirA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (19/24);  total (19/24)

CGGCCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATGAAAATAGGAATCCGGGTCG  >  NC_000913/4173797‑4174268                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                    cggcCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGCTGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAAtc                                                                                                                                                                                                                                >  2:427353/1‑249 (MQ=255)                       aCGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATACCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTcccc                                                                                                                                                                                                         >  2:405250/1‑251 (MQ=255)                                            cGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCt                                                                                                                                                                                    <  1:26881/251‑1 (MQ=255)                                                            gCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGc                                                                                                                                                                     >  2:153306/1‑250 (MQ=255)                                                             cACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCAt                                                                                                                                                                   <  2:581167/251‑1 (MQ=255)                                                                  tATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTGCTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGAt                                                                                                                                                                <  2:322497/249‑1 (MQ=255)                                                                                      gCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTaa                                                                                                                                          <  2:194938/251‑1 (MQ=255)                                                                                      gCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCAGTTGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTaa                                                                                                                                          <  2:42757/251‑1 (MQ=255)                                                                                       cTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTaa                                                                                                                                          >  2:612548/1‑250 (MQ=255)                                                                                              aTTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGATTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGgctc                                                                                                                                  <  2:94033/251‑3 (MQ=255)                                                                                                          gCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAgt                                                                                                                        >  2:419663/1‑249 (MQ=255)                                                                                                          gCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAgt                                                                                                                        >  2:328786/1‑249 (MQ=255)                                                                                                           cccAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAgtagg                                                                                                                     <  2:196236/251‑1 (MQ=255)                                                                                                              aGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAgtaggt                                                                                                                    >  2:149489/1‑249 (MQ=255)                                                                                                                  aaaCTTATCAGTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAata                                                                                                              <  2:210489/251‑1 (MQ=255)                                                                                                                       tATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATAtttt                                                                                                          <  1:419663/250‑1 (MQ=255)                                                                                                                          cATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGAGGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCt                                                                                                       <  2:625808/250‑1 (MQ=255)                                                                                                                              ggTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAg                                                                                                  <  1:194133/251‑1 (MQ=255)                                                                                                                                 gATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTc                                                                                               >  1:306924/1‑251 (MQ=255)                                                                                                                                  aTAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTaa                                                                                                   >  1:524228/1‑246 (MQ=255)                                                                                                                                  aTAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTaa                                                                                                   <  2:524228/246‑1 (MQ=255)                                                                                                                                             tttGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGAtc                                                                                   >  1:107932/1‑251 (MQ=255)                                                                                                                                                  cATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCttt                                                                              <  1:86155/251‑1 (MQ=255)                                                                                                                                                   aTTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTc                                                                             >  1:180332/1‑251 (MQ=255)                                                                                                                                                                    cAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAAGATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCATGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGt                                                            <  1:427353/251‑1 (MQ=255)                                                                                                                                                                             ggCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGc                                                   <  1:328786/251‑1 (MQ=255)                                                                                                                                                                              gCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGct                                                  <  2:580348/251‑1 (MQ=255)                                                                                                                                                                                  tattaCTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGcttct                                               <  2:306924/250‑1 (MQ=255)                                                                                                                                                                                   attaCTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGcttctt                                              <  2:180332/250‑1 (MQ=255)                                                                                                                                                                                          gAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGtt                                      >  1:98203/1‑251 (MQ=255)                                                                                                                                                                                                gATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTc                                <  1:516910/251‑1 (MQ=255)                                                                                                                                                                                                 aTGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTcg                               >  1:471316/1‑251 (MQ=255)                                                                                                                                                                                                           acagcccTGGATGTGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTTCGTAGTCTGACCTCTTCTACCGCATGATTAGCACGTTGCGTCAGGATTAAACTGGCGCGCTCACGAGTAGTTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATg                     <  2:349592/247‑1 (MQ=255)                                                                                                                                                                                                           aaaaCCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTAt                      <  2:486637/250‑1 (MQ=255)                                                                                                                                                                                                            aaaCCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATg                     >  2:224564/1‑250 (MQ=255)                                                                                                                                                                                                            aaaCCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATg                     >  1:96547/1‑250 (MQ=255)                                                                                                                                                                                                                      tGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATGAAAATAGGAAt          >  2:346095/1‑251 (MQ=255)                                                                                                                                                                                                                       gggCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATGAAAATAGGAATc         >  2:504831/1‑251 (MQ=255)                                                                                                                                                                                                                            gTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATGAAAATAGGAATCCGGGt    <  1:346095/251‑1 (MQ=255)                                                                                                                                                                                                                            gTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTGAGTTAATTTCGCGTAGTTATTAAAATAGGAATCCGGGt    <  2:269169/251‑1 (MQ=255)                                                                                                                                                                                                                              gAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATGAAAATAGGAATCCGGGTCg  >  1:509042/1‑251 (MQ=255)                                                                                                                                                                                                                                        tGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAAt                                                      >  2:562281/1‑189 (MQ=255)                                                                                                                                                                                                                                        tGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAAt                                                      <  1:562281/189‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                    CGGCCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATGAAAATAGGAATCCGGGTCG  >  NC_000913/4173797‑4174268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑