Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,123,930	A→T	V176E (GTA→GAA)	cytR ←	DNA‑binding transcriptional repressor CytR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,123,930	0	A	T	100.0%	262.1 / NA	66	V176E (GTA→GAA)	cytR	DNA‑binding transcriptional repressor CytR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (26/40);  total (26/40)

CGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTACTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTG  >  NC_000913/4123697‑4124176                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                         cGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGGGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATgcggcg                                                                                                                                                                                                                                         >  1:530937/1‑251 (MQ=255)       ggCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTc                                                                                                                                                                                                                                      >  1:104123/1‑248 (MQ=255)          agaagaCAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGGCACAACGGCATCTCTTCGGGACCGGCTATACAGCCAATCCCGTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATCCGGCGGttcacttt                                                                                                                                                                                                                                >  2:252207/1‑244 (MQ=255)          agaagaCAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTg                                                                                                                                                                                                                                <  1:125756/251‑1 (MQ=255)                         gCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCCGCTATACAGCCAATCCCGTTATGCCCTTGCTCCTATAAATAATTTTCTGCCTCAAAAGCGGCGGTCAGATTTTCGgtattgacctt                                                                                                                                                                                                                  >  2:237647/1‑243 (MQ=255)                            gTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATg                                                                                                                                                                                                                        <  1:217861/241‑1 (MQ=255)                            gTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGagataggaa                                                                                                                                                                                                               >  2:217861/1‑242 (MQ=255)                                 ggAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGcagctc                                                                                                                                                                                                        <  1:379355/251‑1 (MQ=255)                                 ggAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGcagct                                                                                                                                                                                                         <  1:393725/250‑1 (MQ=255)                                  gAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTTTTGATATTAAAT‑GTAGGcagctcc                                                                                                                                                                                                       >  2:478213/1‑251 (MQ=255)                                             agcTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGGTATGAACT‑TTAGGCAGCTCCAGCTCCggggc                                                                                                                                                                                            >  2:120193/1‑248 (MQ=255)                                                 gctgcATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAAt                                                                                                                                                                                        <  2:294259/251‑1 (MQ=255)                                                    gcATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGAATGTCGATATGAAAT‑GGTGGCAGGTCCAGCTCCGGGGCAAAttcg                                                                                                                                                                                     >  1:320197/1‑251 (MQ=255)                                                     cATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAAttcgt                                                                                                                                                                                    <  2:327909/251‑1 (MQ=255)                                                      aTCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCCGCTCCGGGGCAAAttcgtt                                                                                                                                                                                   >  1:16554/1‑251 (MQ=255)                                                            ttGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCa                                                                                                                                                                              <  2:219051/250‑1 (MQ=255)                                                             tGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCAt                                                                                                                                                                             <  1:253292/250‑1 (MQ=255)                                                             tGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCAt                                                                                                                                                                             <  1:498821/250‑1 (MQ=255)                                                              gCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATc                                                                                                                                                                            <  1:120193/250‑1 (MQ=255)                                                                 tCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑cc                                                                                                                                                                        <  2:332172/250‑1 (MQ=255)                                                                          gAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGGGCAAATTCGGTTGCCATCA‑CCAACggcggc                                                                                                                                                               >  1:300716/1‑250 (MQ=255)                                                                           aaGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGcagctcc                                                                                                                                                                                                       <  1:203604/210‑1 (MQ=255)                                                                           aaGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAg                                                                                                                                                             <  1:31958/251‑1 (MQ=255)                                                                                        cACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCGCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTcc                                                                                                                                                <  1:598659/251‑1 (MQ=255)                                                                                            ggCGGTGTATGGCGGAGCAACCATAAGGCCGCAGCGAAGCAGCGCCTGAACGTAGCCTTGCAGTCGGTAGTGACCCATCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGGTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATTAACT‑GTAGGCAGCTCCAGCTTCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCATATTACGCTGTTCCTc                                                                                                                                              <  2:117655/249‑1 (MQ=255)                                                                                             gCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAAt                                                                                                                                           <  1:566725/251‑1 (MQ=255)                                                                                               gATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTTTCGATATTTACTTGTGGGC‑GCTCCAGCTCCGGTGGAAATTCGGTCGCCATCA‑CCATCCGCGGCAGATTaacctttttctccattc                                                                                                                                         >  1:196368/1‑235 (MQ=255)                                                                                                      tGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCAt                                                                                                                                   <  2:84001/250‑1 (MQ=255)                                                                                                       gCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCCGATTTTTGGTATTAACT‑GTAGGCAGCTCCAGCTCCGGGGCAAATTTCTTTGCCATCAACCA‑CGGCGGCAGATTAAGCTGTTTCTCCATGCCGGCAtac                                                                                                                                 >  2:212509/1‑249 (MQ=255)                                                                                                                        ccGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAAcc                                                                                                                <  2:16554/251‑1 (MQ=255)                                                                                                                               cgaaacttcccTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCAGCAAACGGCAGCCTTGAACCCAGcaac                                                                                                         <  2:555511/242‑1 (MQ=255)                                                                                                                                   cAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAacaaca                                                                                                     <  2:6780/251‑1 (MQ=255)                                                                                                                                      cgcCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGACCCCAGCAACAACATGc                                                                                                  <  1:241959/251‑1 (MQ=255)                                                                                                                                        ccTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGGAGCTCCAGCTCCGGGGCAAAATTGTTCGCCCTCC‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCCTGAACCCCGCCAAAACATGccc                                                                                                >  2:27171/1‑250 (MQ=255)                                                                                                                                        ccTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCa                                                                                                <  1:589760/251‑1 (MQ=255)                                                                                                                                           gAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAAGGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCa                                                                                             <  2:220958/251‑1 (MQ=255)                                                                                                                                               aTAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAAttt                                                                                         <  2:100113/251‑1 (MQ=255)                                                                                                                                                 aGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAAttt                                                                                         <  2:441912/249‑1 (MQ=255)                                                                                                                                                 aGCCTTGCAGGCAGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGGGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCAGTTCCTCAATGCTGGCCTCAAACGGCAGCCTTGAACCCAGCAACAACATGCCAACAAttt                                                                                         >  1:441912/1‑249 (MQ=255)                                                                                                                                                         aGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGtgat                                                                               <  2:104123/251‑1 (MQ=255)                                                                                                                                                          ggCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGtgatg                                                                              <  2:139752/251‑1 (MQ=255)                                                                                                                                                             ggTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGAGGATc                                                                           <  1:100945/251‑1 (MQ=255)                                                                                                                                                                      acaGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTTCGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAAAATGCCATCAATTTGCTTGGTGATGATCAAATCGAt                                                                   >  2:247297/1‑250 (MQ=255)                                                                                                                                                                      acaGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATa                                                                  <  1:359443/251‑1 (MQ=255)                                                                                                                                                                       caGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGGCGTTATGAACT‑GGAGGCACCTCCAGCCCCGGTGCAAATTCGTTCGCCATCA‑CCATCCGCGGCCGATTACGCTGTTCCTCAATGCTCGCATCAAAAGGCAGCCTTGAACCCAGCAACAACATGCCCTCAATTTGCTTGGCGATGAGCAAATccgaa                                                                  >  2:597616/1‑246 (MQ=255)                                                                                                                                                                        aGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATaa                                                                 >  1:206491/1‑250 (MQ=255)                                                                                                                                                                        aGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGGGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGGTGATCAAATCGATaa                                                                 >  1:371828/1‑250 (MQ=255)                                                                                                                                                                          cGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTTTCCATATGAACT‑GTAGGCAGCTCCAGCTCCGGGGCACATTCGTTCGCCATCA‑CCATCGGCGGCCGATTACGCTGTTCCTCaa                                                                                                                                            >  1:406325/1‑173 (MQ=255)                                                                                                                                                                          cGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCaa                                                                                                                                            <  2:406325/173‑1 (MQ=255)                                                                                                                                                                              aTCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGtttt                                                          >  1:486899/1‑251 (MQ=255)                                                                                                                                                                                     cGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTTAACCCAGCAACAACATTCCATCAAGTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCctg                                                    <  1:614147/250‑1 (MQ=255)                                                                                                                                                                                     cGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTTGTGATGATCAAATCGGTAAAGGTTTTTTTctgc                                                   >  2:313248/1‑251 (MQ=255)                                                                                                                                                                                     cGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCctg                                                    <  1:478374/250‑1 (MQ=255)                                                                                                                                                                                      gggACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCctgct                                                  <  1:353578/251‑1 (MQ=255)                                                                                                                                                                                       ggACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCctgctg                                                 <  1:277961/251‑1 (MQ=255)                                                                                                                                                                                          ccGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGGGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCCGCAACAACATGCCATCAATTTTCTTGGGGATTATCAAATTGTTAAAGGTTTTTTCCTGCTGAtt                                              >  2:630749/1‑251 (MQ=255)                                                                                                                                                                                             gCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTg                                           <  2:16022/251‑1 (MQ=255)                                                                                                                                                                                                     gCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGcac                                   <  1:473370/251‑1 (MQ=255)                                                                                                                                                                                                         aTCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAAAT‑GTAGGCAGCTCCAACTCCGGGGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTTCTGCTGATTTTGATGCGCACAGTc                               >  2:54655/1‑251 (MQ=255)                                                                                                                                                                                                                    cccTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCa                    <  1:78623/251‑1 (MQ=255)                                                                                                                                                                                                                         gCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATTAACT‑GTAGGCAGCTCCAGCTCCGGGGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATTCTGGCATCAAACCGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGGGATGATCAAATCGATAAAGGGTTTTTCCTGCTGATTTTGATGCGCCCAGTCACCAATCAGCaac                  >  2:511798/1‑246 (MQ=255)                                                                                                                                                                                                                         gCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAg                <  2:73563/250‑1 (MQ=255)                                                                                                                                                                                                                                 aaataaTTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGt        <  1:401674/250‑1 (MQ=255)                                                                                                                                                                                                                                   ataatTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGGAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGAt     <  1:365786/251‑1 (MQ=255)                                                                                                                                                                                                                                      atTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTTAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGGGAtttt  >  2:24527/1‑250 (MQ=255)                                                                                                                                                                                                                                      atTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCAACAGATATCCGTGATTTg  >  1:493289/1‑251 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                         CGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTACTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACT‑GTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCA‑CCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTG  >  NC_000913/4123697‑4124176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑