Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,468,768	C→T	W362* (TGG→TGA)	chiA ←	endochitinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,468,768	0	C	T	100.0%	226.0 / NA	60	W362* (TGG→TGA)	chiA	endochitinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/26);  total (34/26)

CCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGT  >  NC_000913/3468523‑3469013                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                          ccccATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATATACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGc                                                                                                                                                                                                                                                      <  1:167331/251‑1 (MQ=255)  cccATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCg                                                                                                                                                                                                                                                     >  1:328349/1‑251 (MQ=255)       gCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCtt                                                                                                                                                                                                                                                <  1:28062/251‑1 (MQ=255)       gCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCtt                                                                                                                                                                                                                                                <  1:425615/251‑1 (MQ=255)             tGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTa                                                                                                                                                                                                                                             <  1:576900/248‑1 (MQ=255)                 cGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑Att                                                                                                                                                                                                                                      >  1:384101/1‑250 (MQ=255)                      gAAGTTTGATAATGTGCCGTGTTATAATCTACGATGCTGTCCGCGCTGTCCGCTTTGCTGGCCTGGAAACCACCCGCAACAATGACTCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGAGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTTCCAtt                                                                                                                                                                                                                                <  2:429919/251‑1 (MQ=255)                        aGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTg                                                                                                                                                                                                                               >  1:48700/1‑250 (MQ=255)                         gTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGa                                                                                                                                                                                                                              >  1:619226/1‑250 (MQ=255)                                aaTGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACa                                                                                                                                                                                                                      <  1:228026/251‑1 (MQ=255)                                 aTGTGCATCGTTATAATCTACGCTGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCAGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTAACATTGAAAATAACAt                                                                                                                                                                                                                     >  2:177381/1‑251 (MQ=255)                                    ggCATCGTTATAATCTACGATGCTGTCCGCGCTGAACGCTTTGCGGGCCTGGAAACCATCCGCAACAATGCCGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATgg                                                                                                                                                                                                                   <  2:432895/249‑1 (MQ=255)                                      cATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTa                                                                                                                                                                                                                 >  1:368175/1‑250 (MQ=255)                                      cATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTa                                                                                                                                                                                                                 >  2:195793/1‑250 (MQ=255)                                         cGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTTTTCTCTCGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAT‑ATTACCGTTAAAAATAACATggcatatt                                                                                                                                                                                                             >  2:52495/1‑245 (MQ=255)                                                      gATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑t                                                                                                                                                                                              >  2:351048/1‑251 (MQ=255)                                                      gATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGGAAATTTC‑GCTACCTTCC‑t                                                                                                                                                                                              >  2:620299/1‑251 (MQ=255)                                                       aTGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑Tg                                                                                                                                                                                             <  1:456480/251‑1 (MQ=255)                                                        tGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑Tgg                                                                                                                                                                                            >  1:592537/1‑251 (MQ=255)                                                                  gcTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACg                                                                                                                                                                                  >  2:114317/1‑251 (MQ=255)                                                                       aCGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGc                                                                                                                                                                             <  1:114317/251‑1 (MQ=255)                                                                                         aaCCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACg                                                                                                                                                           >  1:91706/1‑251 (MQ=255)                                                                                         aaCCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACg                                                                                                                                                           <  1:195793/251‑1 (MQ=255)                                                                                               ccGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑TCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATc                                                                                                                                                      <  1:133069/250‑1 (MQ=255)                                                                                                cGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCg                                                                                                                                                    <  2:253580/251‑1 (MQ=255)                                                                                                       aTGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAGC‑ATGTCCAGTGAGAATATCATGGTAAATTAC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGgtgt                                                                                                                                             <  1:576088/251‑1 (MQ=255)                                                                                                                       cAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTa                                                                                                                             <  1:528515/251‑1 (MQ=255)                                                                                                                       cAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTa                                                                                                                             <  1:428358/251‑1 (MQ=255)                                                                                                                           ttCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATcc                                                                                                                          <  1:620299/250‑1 (MQ=255)                                                                                                                                     gTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGt                                                                                                               >  2:597536/1‑251 (MQ=255)                                                                                                                                        gggTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCAc                                                                                                            >  1:55634/1‑251 (MQ=255)                                                                                                                                        gggTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCAc                                                                                                            <  1:318392/251‑1 (MQ=255)                                                                                                                                            tACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGg‑t                                                                                                       >  1:566789/1‑251 (MQ=255)                                                                                                                                                  aCTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTcc                                                                                                 <  2:592537/251‑1 (MQ=255)                                                                                                                                                  aCTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTcc                                                                                                 <  1:597536/251‑1 (MQ=255)                                                                                                                                                  aCTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTcc                                                                                                 >  1:556284/1‑251 (MQ=255)                                                                                                                                                      aCTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGCGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCgg                                                                                               >  2:316717/1‑249 (MQ=255)                                                                                                                                                         cAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGtt                                                                                           <  2:562450/250‑1 (MQ=255)                                                                                                                                                              ccGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGTAGTTGGATc                                                                                      <  2:556284/250‑1 (MQ=255)                                                                                                                                                                    cGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGt                                                                                                                     <  2:234622/214‑1 (MQ=255)                                                                                                                                                                    cGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGt                                                                                                                     >  1:234622/1‑214 (MQ=255)                                                                                                                                                                     ggTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCgg                                                                                >  1:359146/1‑249 (MQ=255)                                                                                                                                                                     ggTAGCTGTAAGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGAAATGCGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑GGTTTCCGGAGTTGGATCAACCgg                                                                                >  2:356255/1‑249 (MQ=255)                                                                                                                                                                         gCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTcac                                                                          >  2:194556/1‑251 (MQ=255)                                                                                                                                                                              aCGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGCTGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCAc                                                                     >  2:130192/1‑251 (MQ=255)                                                                                                                                                                                     agaCGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCatt                                                              >  2:550323/1‑251 (MQ=255)                                                                                                                                                                                       aCGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCattat                                                            >  2:236040/1‑251 (MQ=255)                                                                                                                                                                                            aTGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAAt                                                                                                                                                                   >  2:533606/1‑144 (MQ=255)                                                                                                                                                                                            aTGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTGGCAATGAGTAAt                                                                                                                                                                   <  1:533606/144‑1 (MQ=255)                                                                                                                                                                                                   nttGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTa                                                 >  2:280766/2‑250 (MQ=255)                                                                                                                                                                                                         gTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAAc                                          >  2:357600/1‑251 (MQ=255)                                                                                                                                                                                                               aGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGc                                    >  1:335573/1‑251 (MQ=255)                                                                                                                                                                                                                  gcTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGcc                                   >  2:609031/1‑249 (MQ=255)                                                                                                                                                                                                                       tACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGtt                             >  2:310410/1‑250 (MQ=255)                                                                                                                                                                                                                        ccccggtggccattttttAGCCCCAACCCATCGGGCGTTTTTGAAACC‑ATTACCATTAAAAATAACAGGTTAATTTTC‑TCTCCCTTCC‑TGGCCGCTCCCCGTTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTGGATGGCTCGCTTTTA‑CCGGGGGTGGGGGCCCCGGGTGTTTCCGGGGTTGGATCAACGGGGGTCACAGCTCCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTAc                           <  2:48700/236‑1 (MQ=255)                                                                                                                                                                                                                         ccccGTGGGCAATTTTTAGCCCCACCCCATCAGGCGTT‑TTTTAAACCATTCCCATAGAAAAAAAAATGG‑AAATTTCCGCT‑CCTTCCCGGGCCTCTCCGCGCTTGCAATGGTTAAGCTTTACCGTTTCCCGTTGGGTTTGATGGCGCGCTTTTACCGGGAGTGGGGCCCCCCGT‑TTTTTCGGGGGTTGGACCACCCGGATCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTAcc                          <  2:107972/251‑1 (MQ=255)                                                                                                                                                                                                                           ccGTGGGCAATCTTTAGACCCACCCCATCGGGCGTTCTTGTAAAC‑ATTCCCATTGAAAATAACATGGAAAATTTC‑GCTACCTCCC‑GGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCCCCGG‑TGTTCCCGGAGTTGGATCACCGGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCAt                        <  1:410429/251‑1 (MQ=255)                                                                                                                                                                                                                             gTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATAc                      >  1:52735/1‑251 (MQ=255)                                                                                                                                                                                                                                      cTTTAGACCCAACCCATCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATACGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGAtt             >  2:264838/1‑251 (MQ=255)                                                                                                                                                                                                                                       tttaaccccaccccacCGGGCGTTTTGGTAAAC‑ATTACAATAAAAAATAACAGGGTAAATTTC‑GCTCCCTCCC‑GGGCCGCTCCACGCTTGAATTGAGTAATCGTTACCGCTCCCCGCGGGTGTTGATGGCTCGCTGTTACCCGGAGTGGGGGTCCCGGG‑TTTTTCCGGAGTTGGATCACCGGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGAtt             <  2:566789/244‑1 (MQ=255)                                                                                                                                                                                                                                                 acccaTCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTgttgccgt  >  2:55033/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                          CCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAAC‑ATTACCATTGAAAATAACATGGTAAATTTC‑GCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGT  >  NC_000913/3468523‑3469013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑