Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,177,7480CA33.3% 67.3 / 17.2 49E55* (GAG→TAG) nudFADP‑sugar pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base C (23/9);  new base A (0/16);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.01e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGTGCCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTT‑GGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGG‑CAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTC‑AAAGGGTAGCAAGACTGCGGC‑GTG‑ACCGCGCTCAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTT  >  NC_000913/3177500‑3177988
                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                 
ggcgCCCCCCGGGCTTGCCAGGAATCTTAAC‑CCGGTTTTGGTCCGTTTGACTAGCTTTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCGCCCTCTTCAATCATCCCGG‑CAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCAGCACGCACTGGGTC‑AAAGGGTAGCAAGTCTGCGGC‑GTG‑ACCGCGCTCaa                                                                                                                                                                                                                                                 <  1:532965/248‑1 (MQ=255)
       cacGGGGTTGCGAGAAAACTAAACGCCTGTTG‑GGCCCGTGTGACAATCAGTCACGCATCTTCAATAGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTGCAATCATCCCGG‑CAACCATCTCCAGTAGCCAAGGGGGTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGATCAATCAGCACAACTGCATCACGCACTGGGTC‑AAAGGGTAGCAAGACTGCGGC‑GTG‑ACCGCGCTCAAAAAttta                                                                                                                                                                                                                                          <  2:427/249‑2 (MQ=255)
                  cAGGAAACTTAACACCGGTTT‑GGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGG‑CAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTC‑AAAGGGTAGCAAGACTGCGGC‑GTG‑ACCGCGCTCAAAAATTTCCC‑GCCGCAccc                                                                                                                                                                                                                              >  2:4043/1‑250 (MQ=255)
                             acacCGGTTT‑GGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGG‑CAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTC‑AAAGGGTAGCAAGACTGCGGC‑GTG‑ACCGCGCTCAAAAATTTCCC‑GCCGCACCTCCTGACTCAtt                                                                                                                                                                                                                   >  2:312370/1‑251 (MQ=255)
                                             tttgacctcccccccccGCCTCTTCAATCGCTTCGCGCCGGCCACCATCTTCCACACTTTCCCCCTCTTCAATCACCCCGG‑CAACCATCCCCAGTAGCCAGGGGTTTTCGCTGGTGTCGTCCGCGGCAATCCGAATCTGCTCAACCAGCACAACTTCACCCCACACTGGGTC‑AAAGGGTAACAAGACTGCGGC‑GTG‑CCCGCGCTCAAAAATTCCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAAt                                                                                                                                                                                                       <  2:433103/234‑1 (MQ=255)
                                                tGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGG‑CAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTC‑AAAGGGTAGCAAGACTGCGGC‑GTG‑ACCGCGCTCAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACg                                                                                                                                                                                                  >  1:421949/1‑250 (MQ=255)
                                                 gACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGG‑CAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTC‑AAAGGGTAGCAAGACTGCGGC‑GTG‑ACCGCGCTCAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGAt                                                                                                                                                                                                >  1:555485/1‑251 (MQ=255)
                                                    tATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGG‑CAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTC‑AAAGGGTAGCAAGACTGCGGC‑GTG‑ACCGCGCTCAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGAc                                                                                                                                                                                             >  2:147658/1‑251 (MQ=255)
                                                          ccccGCCGCTCCAACCGCTCCGCGCCGGCCACCTTCTCCCACCCTTTCCCCCTCTTCAATCACCCCGG‑CACCCATCCCCAGTAGCCAGGGGGTTTCGCTGGTGCCCTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCACCACGCACGGGGTC‑AAGGGGTAGCAAGACTGCGGC‑GTG‑ACCGCGCTAAAAAATTCCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATc                                                                                                                                                                                       <  2:254661/250‑1 (MQ=255)
                                                           cccGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGG‑CAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTC‑AAAGGGTAGCAAGACTGCGGC‑GTG‑ACCGCGCTCAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCt                                                                                                                                                                                      >  2:469918/1‑251 (MQ=255)
                                                             cGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGG‑CAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTC‑AAAGGGTAGCAAGACTGCGGC‑GTG‑ACCGCGCTCAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCtat                                                                                                                                                                                    >  1:535260/1‑251 (MQ=255)
                                                             cGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGG‑CAACCATCTCCAGTAGCAAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTC‑AAAGGGTAGCAAGACTGCGGC‑GTG‑ACCGCGCTCAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCtat                                                                                                                                                                                    >  1:468605/1‑251 (MQ=255)
                                                              ccctctccacccGCTCCGCGACGGACAACCTCTTCCACACTTTCCCCCTCTTCAATCACCCCGG‑CAACCATCCCCAGTAGCCAAGGGTTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTC‑AAGGGGTAGCAAGACTGCGGC‑GTG‑CCCGCGCTAAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCtata                                                                                                                                                                                   <  1:102691/240‑1 (MQ=255)
                                                              cccccttaattccctccgcgCCGGGCAACATCTCCCCCCCTTCCACCCTCTTCAACCACCCCGG‑CAACCACCCCCAGTAGCCAGGGGTTTTCGCTGGTGCCGTCCGCGGCAACCCAAATCTGCTCAATCACCACAACTCCATCACGCACTGGGTA‑AAAGGGTAGCAAGCCTGCGGC‑GTG‑CCCGCGCTAAAAAATTCCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCtata                                                                                                                                                                                   <  2:30028/235‑1 (MQ=255)
                                                                    tCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGG‑CAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTC‑AAAGGGTAGCAAGACTGCGGC‑GTG‑ACCGCGCTCAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATaa                                                                                                                                                                                  >  2:593863/1‑246 (MQ=255)
                                                                                  gcggccaataTCTTCCACTCTCTCACCATTTTCTATCATCCCGG‑CACCGACCTCCAGTGGCCAAGGGGTTCCGCTGGTGTCGTGCTGTGCAATCCTAATTTGCTAAATCAACACAACTTAATCCCTCCGTGGGTC‑AAAGGGTAGCAAGCCTGCGGC‑TTG‑ACCGCG‑TTAAAAATTTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGc                                                                                                                                                               <  1:423548/242‑1 (MQ=255)
                                                                                               cccacccttccccccTCTTCAACCGTCCCGG‑CACCCACCCCCAGTCGCAAAGGGGTTTCGGGGTTGTCGTCGGCGGCAACCCGATTCTCCTCAACCACCACACCTTCATCACGCACGGGGTC‑AAGGGGTAGAAAGCCTGCGGC‑GTG‑CCCGCGCTAAAAATTTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAgt                                                                                                                                                    <  1:104029/237‑1 (MQ=255)
                                                                                                  ccctttccACCCTCTTCAATCA‑CCCGGTCAACCACCTCCGGTAGCGAGGGGTTTTCGCTGTTGTCGTACGCGCCAATCCGATTCTGCTCAATCAGCACACCTTCTTCACGCTTTGGGTC‑AAAGGTTAGAAATACTGGGGC‑GGG‑ACGGCGCTCAAAAATTGCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTtc                                                                                                                                               <  1:258184/244‑1 (MQ=255)
                                                                                                  ccacTTTCACCCTCTAAAAGCATCCCGG‑AAACCTCCTCCATTAGCCAGGGGGTTTCGTGGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCCCAACTTCACCACGCCCTGGGTC‑AAAGGGGAGCAAGACTGGGGG‑GTG‑CCCGCGCTAAAAAATTCCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTtc                                                                                                                                               <  1:584096/250‑1 (MQ=255)
                                                                                                         cACCCTCTTCAATCATCCCGG‑CAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTC‑AAAGGGTAGCAAGACTGCGGC‑GTG‑ACCGCGCTCAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGc                                                                                                                                         >  1:624536/1‑250 (MQ=255)
                                                                                                              tctTCAATCATCCCGG‑CAACCATCTACAGTAGACAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTC‑AAAGGGTAGCAAGACTGCGGC‑GTG‑ACCGCGCTCAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGCTGACGAAATCTATAACGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTATCGTGCaataat                                                                                                                                   >  1:497300/1‑251 (MQ=255)
                                                                                                                      caccccGG‑CAACCAGCACCAGTAGCCAAGGTGTTGCGCTGTTGTCGTACGCGACAATCATAATCTGCACAATCAGCACAACTTCATCACGCACTGGGTA‑AAAGGATAGCAAGACTGCG‑T‑GTGGACCGCTCAAAAAAATTTCCC‑GCCGCACCTCATGACTCATTTTCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACAt                                                                                                                           <  2:241032/248‑1 (MQ=255)
                                                                                                                       atcCCGG‑CAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTC‑AAAGGGTAGCAAGACTGCGGC‑GTG‑ACCGCGCTCAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATc                                                                                                                          >  1:104786/1‑251 (MQ=255)
                                                                                                                             g‑CAACCATCCCCAGTAGCCAAGGGGTTTCGCTGGTGTCTTACGCGGCAATCCGAATCTGCCAAACCACCACAACTTCATCCCGCACTGGGTC‑AAGGGGTAGCAAGACTGCGGC‑GTG‑ACCGCGCTCAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGttttt                                                                                                                    <  1:487868/251‑1 (MQ=255)
                                                                                                                                aaCCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTC‑AAAGGGTAGCAAGACTGCGGC‑GTG‑ACCGCGCTCAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGCATAGACGATGACGAAATCTATAAAGATCTAATGCAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGc                                                                                                                  >  1:204278/1‑251 (MQ=255)
                                                                                                                                aaCCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTC‑AAAGGGTAGCAAGACTGCGGC‑GTG‑ACCGCGCTCAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCacgacatctcggaactggttcgt                                                                                                                  >  1:207987/1‑229 (MQ=255)
                                                                                                                                 aCCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTC‑AAAGGGTAGCAAGACTGCGGC‑GTG‑ACCGCGCTCAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGcc                                                                                                                 >  2:327841/1‑251 (MQ=255)
                                                                                                                                   cATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTC‑AAAGGGTAGCAAGACTGCGGC‑GTG‑ACCGCGCTCAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCa                                                                                                                >  2:11085/1‑250 (MQ=255)
                                                                                                                                    aTCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTC‑AAAGGGTAGCAAGACTGCGGC‑GTG‑ACCGCGCTCAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGCTCTa                                                                                                                                                                           >  2:431324/1‑190 (MQ=255)
                                                                                                                                    aTCTCCAGTAGCCAAGGGGTTTCGCGGGGGTCGTACGCGGCAATCCGAATCTGCTCAATCGGCACACCTCCATCACGCACGGGGTC‑AAAGGGTGGCACGCCTGCGGC‑GTG‑ACCGCGCTCAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATTTATAAAGATCTa                                                                                                                                                                           <  1:431324/190‑1 (MQ=255)
                                                                                                                                                          cGCTGGTGTCGTACGCGGCAATCCGATTCTGATCAATCAGCACAACTTCACCCCGCCCGGGGTA‑AAAGGGTAGCAAGACTGGGGC‑GTG‑ACCGCGCTAAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGc                                                                                        <  2:101192/251‑1 (MQ=255)
                                                                                                                                                            gtgtggccgaaCGCGGCAATCCAAATCTGCTCAACCAGCACAACTCCACCCCGCCCGGGGTA‑AAGGGGCAGCAAGACTGCGGC‑TTA‑CCCGCGCTAAAAAATTCCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCtt                                                                                      <  2:421949/241‑1 (MQ=255)
                                                                                                                                                                  ccGTACGCGGCAACCCGAATCTGCTCAACCAGCCCACCTCCACCCCGCACTGGGTC‑AAAGGGTACCAAGACTGCGGC‑GTG‑CCCGCGCTAAAAAATTCCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCAt                                                                                <  1:27072/250‑1 (MQ=255)
                                                                                                                                                                      aggcggcaccccaaatttGCTCAATCAGCCCAATTCCACCACGC‑CTTGGTCCAAGGGGTAGAAAG‑CCGCGGCCGTG‑ACCTCGCACAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGt                                                                             <  1:599171/233‑1 (MQ=255)
                                                                                                                                                                                        gCTCAAACAGCACAACTTCACCCCGCCCTGGGAC‑AAAGGTGAGCAAGACGGCGGC‑GTG‑CCCGCGCTCAAAATTTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATa                                                          <  2:376977/251‑1 (MQ=255)
                                                                                                                                                                                                   caaactccatcccccacggggac‑AAAGGGTAGAAAGCCGGCGGC‑GTA‑CCCGCCCTAAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAAt                                               <  2:613346/229‑1 (MQ=255)
                                                                                                                                                                                                   acaACTTCATCACGCACTGGGTC‑AAAGGGTAGCAAGACTGCGGC‑GTG‑ACCGCGCTCAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAAt                                               >  1:339058/1‑251 (MQ=255)
                                                                                                                                                                                                    caacaccaccacccacggggTC‑AAGGGGTAGCAAGACGGCGGC‑GTC‑ACCGGCCTAAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCAGTGTTAATGTCCGGGCAGTAATAGTGAATTAAAtg                                              <  2:484338/234‑1 (MQ=255)
                                                                                                                                                                                                    caACTTCATCACGCCCGGGGTC‑AAAGGGTAGAAAGACTGCGGC‑GTG‑ACCGCGCTAAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCt                                                                                                                                                                            <  1:460025/125‑1 (MQ=255)
                                                                                                                                                                                                    caACTTCATCACGCACTGGGTC‑AAAGGGTAGCAAGACTGCGGC‑GTG‑ACCGCGCTCAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCt                                                                                                                                                                            >  2:460025/1‑125 (MQ=255)
                                                                                                                                                                                                                           aaaGGGTAGCAAGCCGGCGGC‑TTG‑ACCGCCCTAAAAAATTCCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACt                        <  1:11085/251‑1 (MQ=255)
                                                                                                                                                                                                                                      aGACTGCGGC‑GTG‑ACCGCGCTCAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGc             >  2:532664/1‑251 (MQ=255)
                                                                                                                                                                                                                                        ccTGCGGC‑GTC‑CCCGCCCTAAAAAGTTCCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGATTAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAg           <  2:514120/250‑1 (MQ=255)
                                                                                                                                                                                                                                         cTGCGGC‑GTG‑ACCGCGCTCAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCAt                                                                                >  1:243284/1‑181 (MQ=255)
                                                                                                                                                                                                                                             ggc‑gTG‑ACCGCGCTCAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCAtg      >  2:453777/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 gTG‑ACCGCGCTCAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTg                                                                                          >  2:346372/1‑164 (MQ=255)
                                                                                                                                                                                                                                                 gTG‑ACCGCGCAAAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTg                                                                                          <  1:346372/164‑1 (MQ=255)
                                                                                                                                                                                                                                                   agcccGCTCTCAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAATATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCTTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGtt  <  1:251079/248‑1 (MQ=255)
                                                                                                                                                                                                                                                    gcccGCGCTAAAAAATTCCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGtt  <  2:535260/248‑1 (MQ=255)
                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                 
GGTGCCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTT‑GGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGG‑CAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTC‑AAAGGGTAGCAAGACTGCGGC‑GTG‑ACCGCGCTCAAAAATTTCCC‑GCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTT  >  NC_000913/3177500‑3177988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑