Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,386,8620GA30.8% 40.1 / 12.2 26intergenic (+31/‑72)yfbL/yfbMputative peptidase YfbL/DUF1877 domain‑containing protein YfbM
Reads supporting (aligned to +/- strand):  ref base G (1/17);  new base A (8/0);  total (9/17)
Fisher's exact test for biased strand distribution p-value = 5.76e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGC‑AACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACA‑TTTTATTTT‑CCCTCATCTGTTTTAAGATGCGAAACATGAAATTC‑AACGGAGGAAAAATAAAT‑GGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTTACACT  >  NC_000913/2386634‑2387084
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                  
gggTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGC‑AACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGACAAGCACAATTTTTTTTT‑c                                                                                                                                                                                                             >  1:628721/1‑251 (MQ=255)
    ttATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGC‑AACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAACAACGACaatcccactttttttttcctcc                                                                                                                                                                                                          >  1:8790/1‑230 (MQ=255)
      aTTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGC‑AACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACACGAACa‑tattattttttctcctt                                                                                                                                                                                                       >  2:192674/1‑243 (MQ=255)
       gttctggtgtttttttCTCTAACCCCCTAAATT‑TTGGCCAACACAATATCCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACA‑TTTTATTTT‑CCCTCATCt                                                                                                                                                                                                     <  1:539950/238‑1 (MQ=255)
            ggTATTGATTTCTCTGACCACCTGAATTATTGGC‑AACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGGCAGAAAAAGAGACATACCAA‑ATTTATTTT‑TCCTCAtttatatt                                                                                                                                                                                                >  2:539950/1‑244 (MQ=255)
                                        tatgtgcacctatttatccccGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACA‑TTTTATTTT‑CCCTCATCTGTTTTAAGATGCGAAACATGAAATTC‑AACgg                                                                                                                                                                     <  2:417623/232‑1 (MQ=255)
                                                 cacGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTTCCGCAAAAAAGACAAAAAAA‑CTTTATTTT‑TCCTCCTCTTTTTTATGATGCGAAACATGAAAATA‑AACTGAGGaaaaaa                                                                                                                                                            >  2:484967/1‑249 (MQ=255)
                                                   atatactaccGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACA‑TTTTATTTT‑CCCTCATCTGTTTTAAGATGCGAAACATGAAATTC‑AACGGAGGAAaaataaa                                                                                                                                                         <  2:531637/243‑1 (MQ=255)
                                                         ccccGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACA‑TTTTATTTC‑CCCTCATCTGTTTTAAGATGCGAAACATGAAATTC‑AACGGAGGAAAAATAAAT‑GGGaa                                                                                                                                                  <  2:566573/250‑1 (MQ=255)
                                                           ccGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGCCATGCACA‑TTTTTTTTT‑TCCTCCTCTGTTTTTAAATGCGAAACAAGAAATTT‑AAAGGAGGGaaaaaaaaaaggaat                                                                                                                                                  >  1:109300/1‑243 (MQ=255)
                                                                  aaTGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTAAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCAAGGGGGGGCCAGAAAAAAGGAAAAGCACA‑TTTTTTTTTTCCCT‑TTTTTTTTTTAGATGCGAAAAAAGAAATTT‑AAAGGGGGAaaaaaaaaagtgaagattttggt                                                                                                                                          >  1:601766/1‑236 (MQ=255)
                                                                     gATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACA‑TTTTATTTT‑CCCTCATCTGTTTTAAGATGCGAAACATGAAATTC‑AACGGAGGAAAAATAAAT‑GGGAATGATTGGCTAtt                                                                                                                                      <  1:226263/251‑1 (MQ=255)
                                                                           tGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACATGCACA‑ATTTATTTT‑TCCTCTTCTTTTTTTTGATGCGGAACATGAAAATCTAACGG‑GGAAAAAAAAAT‑GGGGAAGATTGGGTAtttttc                                                                                                                                  >  1:367800/1‑247 (MQ=255)
                                                                            gACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACA‑TTTTATTTT‑CCCTCATCTGTTTTAAGATGCGAAACATGAAATTC‑AACGGAGGAAAAATAAAT‑GGGAATGATTGGCTATTTTGCa                                                                                                                                 <  2:498313/249‑1 (MQ=255)
                                                                                        tATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGGCATGCACA‑TTTGATTTT‑CCCTCATCTGTTTTAAGATGCGAAACATGAAATTC‑AACGGAGGAAAAATAAAG‑GGGAATGATTGGCTATTTTGCAGAAATTGACTCAg                                                                                                                    <  2:528848/250‑1 (MQ=255)
                                                                                                 aaaCAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTGCAGAAAAAAAAACAAGCAAA‑TTTTTTTTT‑TTCTCATTTTTTTTATAAAGCGACAAATGAAAATT‑AACGGAGGAAAAAAAAAAATGGAA‑GATTGGCTAtttttttgaaaatgactacaaaaaaatta                                                                                                           >  2:489575/1‑225 (MQ=255)
                                                                                                        aCCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACA‑TTTTATTTT‑CCCTCATCTGTTTTAAGATGCGAAACATGAAATTC‑AACGGAGGAAAAATAAAT‑GGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACt                                                                                                    <  2:138821/250‑1 (MQ=255)
                                                                                                                    gTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACA‑TTTTATTTT‑CCCTCATCTGTTTTAAGATGCGAAACATGAAATTC‑AACggagg                                                                                                                                                                  <  1:457777/177‑1 (MQ=255)
                                                                                                                      gATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACA‑TTTTATTTT‑CCCTCATCTGTTTTAAGATGCGAAACATGAAATTC‑AACGGAGGAAAAATAAAT‑GGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTg                                                                                      <  2:475910/250‑1 (MQ=255)
                                                                                                                                                                  ggATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGAGTCCAGAAAAAGCGACATGCACA‑TTTTATTTT‑CCCTCATCTGTTTTAAGATGCGAAACATGAAATTC‑AACGGAGGAAAAATAAAT‑GGGAATGATTTGCTATTTTGCAGAAATTGACTCTGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTAc                                         <  2:268852/251‑1 (MQ=255)
                                                                                                                                                                   gATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACA‑TTTTATTTT‑CCCTCATCTGTTTTAAGATGCGAAACATGAAATTC‑AACGGAGGAAAAATAAAT‑GGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACAGGACACGCTTTCAGGATTACg                                        <  1:527944/251‑1 (MQ=255)
                                                                                                                                                                           tataACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACA‑TTTTATTTT‑CCCTCATCTGTTTTAAGATGCGAAACATGAAATTC‑AACGGAGGAAAAATAAAT‑GGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTg                                 <  1:38593/250‑1 (MQ=255)
                                                                                                                                                                              aaCTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACA‑TTTTATTTT‑CCCTCATCTGTTTTAAGATGCGAAACATGAAATTC‑AACGGAGGAAAAATAAAT‑GGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGat                              <  2:381484/250‑1 (MQ=255)
                                                                                                                                                                               aCTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACA‑TTTTATTTT‑CCCTCATCTGTTTTAAGATGCGAAACATGAAATTC‑AACGGAGGAAAAATAAAT‑GGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCGTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGatat                            <  1:192674/251‑1 (MQ=255)
                                                                                                                                                                                           aacaGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACA‑TTTTATTTT‑CCCTCATCTGTTTTAAGATGCGAAACATGAAATTC‑AACGGAGGAAAAATAAAT‑GGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGTTATTGATAAACGCTg                <  2:221704/251‑1 (MQ=255)
                                                                                                                                                                                                         aaaaTTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACA‑TTTTATTTT‑CCCTCATCTGTTTTAAGATGCGAAACATGAAATTC‑AACGGAGGAAAAATAAAT‑GGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTTACACt  <  2:318990/251‑1 (MQ=255)
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                  
GGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGC‑AACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACA‑TTTTATTTT‑CCCTCATCTGTTTTAAGATGCGAAACATGAAATTC‑AACGGAGGAAAAATAAAT‑GGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTTACACT  >  NC_000913/2386634‑2387084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑