| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,397,830 | 0 | T | C | 31.6% | 116.6 / 10.0 | 57 | I265V (ATT→GTT) | uspE | universal stress protein E |
| Reads supporting (aligned to +/- strand): ref base T (31/8); new base C (0/18); total (31/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.03e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCCCGTTGGGAATTTATTTCCTTGGCGGCGAAGAGCAGTAAGTAAAAAATAGGCCCGATAACTCGGGCCTTGTCAGTTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGG‑TTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTCGGTATGGTTGA > NC_000913/1397581‑1398063 | aTCCCGTTGGGAATTTATTTCCTTGGCGGCGAAGAGCAGTAAGTAAAAAATAGGCCCGATAACTCGGGCCTTGTCAGTTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGG‑TTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATc < 1:37161/251‑1 (MQ=255) ccGTTGGGAATTTATTTCCTTGGCGGCGAAGAGCAGTAAGTAAAAAATAGGCCCGATAACTCGGGCCTTGTCAGTTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGG‑TTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATcccc < 2:313778/251‑1 (MQ=255) cGTTGGGAATTTATTTCCTTGGCGGCGAAGAGCAGTAAGTAAAAAATAGGCCCGATAACTCGGGCCTTGTCAGTTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGG‑TTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCg > 1:114138/1‑251 (MQ=255) tttCGTTGGCGGCAAAGGACAGTAAGAAAAAAATGGGCCCGATACCTCGTCCTTTGTCAGTTATGGAAGCGTCGTTAATCGTCTTCTTCGTCATCCAGTCCACCGGGTGTCTGATACTGGTGAGT‑TTTAATGCCCAGCAGGTCGCGGCAAAGATGCTCAATCCCCTTTC‑CCGCCGTGTTCCCGAGGAATGCTGCTGAAATCCCGGGGC‑G‑TCCTCCCGTCCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCt < 2:434684/250‑1 (MQ=255) ttGGCGGCGAAGAGCAGTAAGTAAAAAATAGGCCCGATAACTCGGGCCTTGTCAGTTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCCACGGGTGTCTGATACTGGTCAGG‑TTTAATGACCAGCAGGACGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTCCCGAGGAATGCTGCTGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGcc > 2:260542/1‑250 (MQ=255) aattaagaaaAAAATAGGCCCAAAAACTCGGGCTTTTTCATTTATTGAGGTGTCTTTATTCGTCTTTTTCGTCACCCAGTTCAGGGGGTTTCTGATCCTGGTCAGG‑TTAAACCACCAGCAGGTCGCAGCGAAGATGATAAACCCCCTGTC‑CCGCCGTTTGGCCGAGGAATGCTGCTAAAATCCCGG‑GC‑GGTCCTCCCGTCCCCAGACCC‑CCAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCAcc < 2:398172/248‑1 (MQ=255) agtaagtaAAAAATAGGCCCGATAACTCGGGCCTTGTCAGTTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGG‑TTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTg > 1:526388/1‑222 (MQ=255) agtaagtaAAAAATAGGCCCGATAACTCGGGCCTTGTCAGTTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGG‑TTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTg < 2:526388/222‑1 (MQ=255) aaagaaaaaaaTGGCCCCGATACCTGGGGCTTTGTCAGTTATAGAAGGTTCTTTAATCGTTTTCTTCGTAATCCATTGCAACGGGTTTCTGAT‑CCGGTTAGGGTTAAATGCCCAGCGGGTCGCGGGGAGGAGGATCAATCCCCTTTT‑CCGCCGTGTTGCCGGGGAGTGCTGTTAAAACCCGGGTGC‑G‑TCCTCCCGGGCCCAAACCC‑ACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACctct < 1:90267/246‑1 (MQ=255) aaggccccaaaaaccgggtctttGTCAGTTTTGGAAGATTCGTAAATCGTCTTCTTCGTCACCCAGTTCAAGGGGTGTCTGATCCTGGTCAGG‑TTAAATGACCAGCGGGCCGCGGCAAAGATAATCAATCCCCTGTC‑CCGCGGTTTTGCCGAGGAATGCTGCTAAAAT‑CCGGGGCCG‑CCCTCCCGTCCCCAAACCC‑ACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGAc < 2:522949/230‑1 (MQ=255) tAACTCGGGCCTTGTCAGTTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGG‑TTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTAc > 2:85791/1‑251 (MQ=255) ccTCGGGCTTTGTCAGTTTTTGAAGTGTCTATAAGCTTCTTCTGCGTCATCCAGTTCAAGGGTTGTCTGATACTGGTCTGG‑TTGAATGACCAGCGGGTCGCAGCGAATAGGACCAACCCCCTTTC‑CCGCGGTTTTCCCGGGGAATCCTGCTAAAATACCGGTGC‑G‑TCCTCCCGTCCCCAGAACC‑ACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGt < 1:216234/250‑1 (MQ=255) ggCCTTGTCAGTTATTGAAGAGTCTTTAATCGTCTTCTTCGCCACCCAGTTCAACGGGTTTCTGATACTGGTCAGG‑TTAAATGCCCAGCGGGTCGCAGCGAAGATGATCAATCCCCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTAAAATCCCGGTGC‑G‑TCCTACCGGGCCCAGAACC‑ACAACCCCCGCCTGTAAGTGCTCCGCCAAAt < 1:227871/210‑1 (MQ=255) ggCCTTGTCAGTTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGG‑TTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAAt > 2:227871/1‑210 (MQ=255) aatcggTCATTTATTGAAGAGTCTTAAATCTTTTTCTTCGTCATCCAGTTCAACGGGTTTCTGATACTGGTCAGG‑TTTAATGCCCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTC‑CCGCCGTGTTGCCGAGGAATGCTGCTAAAATCCCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCa < 1:185928/246‑1 (MQ=255) gTCATTTATTTAGGAGTCTTTAATCGTTTTTTTCGCCATCCAGTGCAGCGGGTGTCTGATACTGGTCAGG‑TTTAATGACCAGCAGGTCGCGGCAAAGATGATCAATCCCCTGTC‑CCGCCGTGTGGCCGAGGAATGCTGCTAAAATCCCGGTGC‑G‑TCCTCCCGTGCCCAGAACC‑ACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGtt < 2:114138/250‑1 (MQ=255) gTCAGTTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGG‑TTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCa > 2:185928/1‑246 (MQ=255) cgctctccctatctgtaattCGTCTTCTTCGTCACCCAGTACAACGGGGGTCTGATACTGTTCAGT‑TTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCCCCTTTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATCCCGGGGC‑T‑CCCTCCCGTGCCCAAACCC‑ACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCAt < 2:590096/232‑1 (MQ=255) agagTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGG‑TTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCg > 1:573969/1‑251 (MQ=255) agagGCGTTAAGCGTCTTCTTCGTCATCCAGTTAAACGGGTGTCTGATACTGGTCAGG‑TTTAATGACCGGCAGGGCGCAGCGAAGATGATCAATCCCCTGTT‑CCGCCGTGTTGCCGAGGAAGGCTGCTGAAATCCCGGTGC‑G‑TCCTACCGTGCCAAGACCC‑CCAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTCTCATTAATGCCg < 1:443778/251‑1 (MQ=255) agTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGG‑TTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGa > 2:346991/1‑250 (MQ=255) gTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGG‑TTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCAt > 1:590096/1‑237 (MQ=255) gTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGG‑TTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTc > 2:347895/1‑251 (MQ=255) tAATCGTCTTCTTCGTCACCCAGTTCAGCGGGTGTCTGATACGGGTCGGG‑TTTAATGACCAGCGGGGCGCAGCGAAGATAATCAATCCCCTGTC‑CCGCCGTTTTGCCGAGGAATGCTGCTAAAATCCCGGTGC‑G‑CCCTACCGTCCCCAAACCC‑ACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCt < 2:159945/250‑1 (MQ=255) tgtcaacccAGTTCAACGGGTGTCGGATACTGGTAAGG‑TTTAATGCCCAGCAGGTCGCGGCGAAGATGACCAATCCCCTTTC‑CCGCCGTGTTCCCGAGGAATGCTGCTAAAATCCCGGGGC‑G‑TCCACCCGT‑CCCAAAACCCACACTCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCt < 2:74880/242‑1 (MQ=255) aGTTCAACGGGTGTCTGATACTGGTCAGG‑TTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGcc > 2:333955/1‑249 (MQ=255) gtgtCTGATACTGGTCAGG‑TTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGACTTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGc > 1:517620/1‑251 (MQ=255) gtCTGATATTGGTCAGT‑TTTAATGCCCAGCGGGTCGCAGCGAAGATGATCAATCCCCTGTC‑CCGCCGTGTTGCGGAGGATGGCTGCTGAAATCCCGGTGC‑G‑CCCTCCCTTCCCCAGACCC‑CCAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGccc < 1:587621/251‑1 (MQ=255) cTGGTCAGT‑TTAAATGACCAGCGGGTCGCAGCGAAGATGATCAACCCCCTGTC‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATCCCGGTGC‑G‑TCCTCCCGTGCCCAGACCC‑ACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCAtt < 1:10982/226‑1 (MQ=255) cTGGTCAGG‑TTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCAtt > 2:10982/1‑226 (MQ=255) ccggcagGTCGAAGCGAAGATGTTAAATCCCCTGTT‑CCGCGGTGTGGCCGAGAATTGCTGCTAAAATACCGGTGC‑G‑TCCTACCGGGCCCAGACCC‑CCAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTc < 1:61208/248‑1 (MQ=255) cagcagGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCg > 1:427695/1‑251 (MQ=255) ggTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTc > 1:384947/1‑250 (MQ=255) aGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATACACGCTCGGGTCAAATTcc > 2:233342/1‑251 (MQ=255) aGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTcc > 2:466965/1‑251 (MQ=255) ggAAGATGATCAATCCCCTGTC‑CGGCCGTGTTGCCGAGGAAGGCTGCTGAAATACCGGGGC‑G‑CCCTACCGTGCCCAGACCC‑ACACCCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCTCCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCgg < 1:347895/250‑1 (MQ=255) aatcaCCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATcg > 2:97753/1‑251 (MQ=255) tcccTGTTGCCGCCGTGTTGCCGGGGAATGCTGCAGAAATCCCGGTGC‑C‑CCCTACCGTGCCCAGACCC‑CCAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATcgc < 2:407942/248‑1 (MQ=255) caCCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATcgcg > 1:414321/1‑250 (MQ=255) ccTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGat > 1:122248/1‑250 (MQ=255) cgccgTGTTGCCGAGGAATGCTGCTGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTg > 1:494683/1‑251 (MQ=255) cGAGGAATGCTGCTGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCgg > 1:585504/1‑250 (MQ=255) gAGGAATGCTGCTGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGAt > 1:143305/1‑251 (MQ=255) gctgctGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGcc > 2:233395/1‑251 (MQ=255) gctaaaaTACCGGTGC‑G‑TCCTACCGTGCCCAGACCC‑ACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCa < 1:333955/245‑1 (MQ=255) aaatcccgggcggccctcccGTCCCCAGAACC‑AAAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGa < 2:122248/233‑1 (MQ=255) tACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATg > 1:177692/1‑250 (MQ=255) ggTGC‑G‑TCCTCCCGTGCCCAGACCC‑ACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACtt < 1:97753/251‑1 (MQ=255) ggTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACtt > 1:282315/1‑251 (MQ=255) c‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTCGGt > 2:608137/1‑251 (MQ=255) ntCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTg > 2:290771/2‑213 (MQ=255) ccTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATgg > 2:138990/1‑157 (MQ=255) ccTACCGCGCCCAGACCC‑AAAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTCGGTAt < 1:346991/250‑1 (MQ=255) cTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTCGGTATgg > 2:587367/1‑251 (MQ=255) cgcgCCCAGACCC‑ACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCCACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTCGGTATGGTTGa < 1:544017/248‑1 (MQ=255) aaTCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGtt > 2:10255/1‑173 (MQ=255) aaTCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGtt < 1:10255/173‑1 (MQ=255) | ATCCCGTTGGGAATTTATTTCCTTGGCGGCGAAGAGCAGTAAGTAAAAAATAGGCCCGATAACTCGGGCCTTGTCAGTTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGG‑TTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGC‑G‑TCCTACCGTGCCCAGAACC‑ACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTCGGTATGGTTGA > NC_000913/1397581‑1398063 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |