Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913599,5760TG48.6% 33.1 / 16.3 35V288G (GTG→GGG) cusAcopper/silver export system RND permease
Reads supporting (aligned to +/- strand):  ref base T (1/17);  new base G (17/0);  total (18/17)
Fisher's exact test for biased strand distribution p-value = 4.19e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GATGCTTCAAACCAGGAAGCGGGCGGTTCGTCGATCGAACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGC‑‑AAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACCTCAGCGG‑CAAGTTGCTGGAAGAG‑TTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGC  >  NC_000913/599344‑599824
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                            
gATGCTTCAAACCAGGAAGCGGGCGGTTCGTCGATCGAACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCgg                                                                                                                                                                                                                                            >  1:68007/1‑251 (MQ=255)
 aTGCTTCAAACCAGGAAGCGGGCGGTTCGTCGATCGAACTGGCGGCAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGATCCTGCGAGCCGGc                                                                                                                                                                                                                                           >  1:359885/1‑251 (MQ=255)
      tCAAACCAGGAAGCGGGCGGTTCGTCGATCGAACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGTTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCTAAGGCGAAGTGGCGGGCGGGGTGGTTATCCTGCGATCCGGC‑‑aaa                                                                                                                                                                                                                                      <  1:22735/249‑1 (MQ=255)
                        ggTTCGTCGATCGAACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCCGCGATCCCGC‑‑AAAAAAGCCCGAGAAgggaatgc                                                                                                                                                                                                                  >  1:89106/1‑244 (MQ=255)
                                   cgaAGTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGGCTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGTTGGTGATCCTGCGATCCGGC‑‑AAAAGCGCCCGAGAAGTGATCGCCGCCGTGAAgg                                                                                                                                                                                                       <  2:575587/251‑1 (MQ=255)
                                    gaACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGGCCTGCGATCCAGCGAAAAAACCCCCgagagggacgcagccccggaggg                                                                                                                                                                                                        >  2:155782/1‑232 (MQ=255)
                                         ggCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGATCCTGCGGTCCGGC‑‑AAAAAAGCCCGCGAAGGGAACGCCGCCGCGGAGGACaaa                                                                                                                                                                                                  >  1:410829/1‑250 (MQ=255)
                                                 ccttcctatctgTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGC‑‑AAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACg                                                                                                                                                                                         <  2:506434/240‑1 (MQ=255)
                                                       atatGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGATTCCGC‑‑AAAAAAGCCCGAGAAGGGGTTGCCGCCGCGGAGGGCAAACCGGGAACGCTGaa                                                                                                                                                                                    >  1:214811/1‑250 (MQ=255)
                                                         atGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGC‑‑AAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGaaaa                                                                                                                                                                                  <  2:314505/250‑1 (MQ=255)
                                                            gTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGAACCGGC‑‑CAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTAGAAACg                                                                                                                                                                                         >  1:506434/1‑240 (MQ=255)
                                                                       cGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGC‑‑AAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAg                                                                                                                                                                   <  2:84103/251‑1 (MQ=255)
                                                                                   aaCGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGC‑‑AAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAg                                                                                                                                                       <  1:155782/251‑1 (MQ=255)
                                                                                               cTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGGTCCGGG‑‑AAAAACGCCCGGGAAGGGGTCGCCGCCGGGGAGGGCAAAATGGAAAAGCTGGAAAGGAGGCTGCCGGGAGGCGGGGAGGGAGTTACAACATACg                                                                                                                                           >  1:156210/1‑251 (MQ=255)
                                                                                                tttAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGGGATCCTGCGGTCCGGC‑‑AAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGGAGTCTGCCGGAAGGCGGGGGGATAGTTACAACATACg                                                                                                                                           >  1:193416/1‑250 (MQ=255)
                                                                                                  tAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGC‑‑AAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGAt                                                                                                                                         <  2:359885/250‑1 (MQ=255)
                                                                                                      cacaTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGAGTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGC‑‑AAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGcag                                                                                                                                    <  2:24634/251‑1 (MQ=255)
                                                                                                       acaTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGC‑‑AAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGcagc                                                                                                                                   <  2:10173/251‑1 (MQ=255)
                                                                                                                         gTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGC‑‑AAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGcc                                                                                                                 <  1:338/251‑1 (MQ=255)
                                                                                                                             aaaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTTCTGCGGTCCGGC‑‑AAAAAAGCCCGAGAAGGGATCGCCGCCCGGGAGGACAAACTGGGAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGGGATAGGTACAACATACGATCCCAGCCCGCTCACTGACCGCGCCaccg                                                                                                             >  2:404195/1‑248 (MQ=255)
                                                                                                                                       cccGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGC‑‑AAAAAAGCCCGCGAAGTGATCGCCGCCCTGGAGGACAAACTTGAAACGCTGTAAAGGAAGCTGGCGGGAAGCGGGGTGATAGTTACAACATACGCACGCATCCAGGACATTGACAGCGGCCTCTACAACCTCac                                                                                                   >  1:440259/1‑250 (MQ=255)
                                                                                                                                        ccGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGAGCCGGGGCAGTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGTGGTGATCCTGCGATCCGGC‑‑AAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACCTCAGc                                                                                                  <  1:356328/251‑1 (MQ=255)
                                                                                                                                                  gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGC‑‑AAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACCTCAGCGG‑CAAGtt                                                                                         <  1:6102/249‑1 (MQ=255)
                                                                                                                                                  gcgcGAGGTTGCGAAGGGCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGC‑‑AAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACCTCAGCGG‑CAAGtt                                                                                         <  2:433216/249‑1 (MQ=255)
                                                                                                                                                      gATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGACCTGCGATCCGGC‑‑AAAAACGCCCGCGAAGGGGTAGCCGCCGTGAAGGGCAAACTGGGAACGCTGGAAAATAGTCTGCCGGGAGGCGGGGGGAAGGTTACAAAAAACGAACCCCGCCAGCTCCATGACCCCGCCATCGACaaaccccacggcaaggtcttggg                                                                                    >  2:547940/1‑230 (MQ=255)
                                                                                                                                                              gAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGC‑‑AAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACCTCAGCGG‑CAAGTTGCTGGAaga                                                                                <  2:454580/246‑1 (MQ=255)
                                                                                                                                                              gAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGGTCCTGCGATCCGGC‑‑AAAAACGCCCGAGAAGTGATCGCACCCGTGAAGGACAAACTGGAAACGCTGAAAAATAGTCTGCCGGGAGGCGTGGAGAAAGTTTCAACATACGATCGCAGCCAGCTCATTTACCGCGCCATCGACAAACTCAGCGGGCAAGT‑GCAGGAagaggttt                                                                           >  1:292593/1‑247 (MQ=255)
                                                                                                                                                              gAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTGCGATCCGGC‑‑AAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAAAGCTGAAAAGTAGTCTGCCGGGAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCCTTGACCGCGCCATCGACAACCTCAGCGG‑CCAGTTGCTGGAaga                                                                                >  1:454580/1‑246 (MQ=255)
                                                                                                                                                                      aGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCCGCGGTTCGGC‑‑CAAAACGCCCGCGAAGGGATCGCCGCCGTGGAGGGAAAACAGGAAACGCTGGAAAATAGTCTGCCCGGAGGCGGGGGGATAGTTACAAAAAACGATCGCAGCCAGCTCCTTTACCGCGCCCTCGCCAACCCCCGCGG‑CACGTTGGTGGAAGAA‑TTTTTTttggtg                                                                  >  2:391814/1‑251 (MQ=255)
                                                                                                                                                                           ggCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGC‑‑AAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACCTCAGCGG‑CAAGTTGCTGGAAGAG‑TTTATTGTGGTGGCgg                                                              <  2:193416/250‑1 (MQ=255)
                                                                                                                                                                           ggCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGC‑‑AAAAACGCCCGGGAAGGGATCGCCGCCGGGAAGGACAAACTGGAAAAGCTGAAAAGTAGTCTGCCGGAAGGGGGGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACCTCAGCGG‑CAAGTTGCTGGAAGAG‑GTTATTGTGGGGGCggg                                                             >  1:457875/1‑250 (MQ=255)
                                                                                                                                                                                           ggggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGC‑‑AAAAAAGCCCGAGAAGTGAACGCCGCCGTGAAGGACAAAATGGAAACGCTGAAAAGTAGTCTGCCGGAAGGGGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGAACGCGCCATCGACAACCTCAACGG‑CAAGGTGGTGGAAGAGGTTTAT‑GTGGTGGCGGGAGGCTTTGCGCTGttt                                             >  1:178988/1‑251 (MQ=255)
                                                                                                                                                                                                                     ggcgaagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGC‑‑AAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACCTCAGCGG‑CAAGTTGCTGGAAGAG‑TTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCtggt                   <  1:535440/251‑1 (MQ=255)
                                                                                                                                                                                                                       cgaagTGGCGGGCGGGGGGGGGATCCCGCGATCCGGC‑‑AAAAACGCCCGAGAAGTGATCGCCGCCGGGAAGGAAAAACTGGAAACGCTGAAAAGAAGTCTGCCGGGAGGGGGGGAGGTAGTTACAACATAAGATCGCAGCCAGCTCATTTACCGCGCCCTCGACAACCTCAGCGG‑CAAGGTGCTGGAAGAG‑GTTTTTGTGGTGGCGGGAGTCTTTGCGCGGTTTttctcggctgaaagcggcaaaggcgca                  >  1:557929/1‑227 (MQ=255)
                                                                                                                                                                                                                         aagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGC‑‑AAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACCTCAGCGG‑CAAGTTGCTGGAAGAG‑TTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCg               <  1:226158/251‑1 (MQ=255)
                                                                                                                                                                                                                               ggggggggttggtgATCCTGAGATCCGGC‑‑AAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACCTCAGCGG‑CAAGTTGCTGGAAGAG‑TTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGattatt         <  2:459768/242‑1 (MQ=255)
                                                                                                                                                                                                                                      ggtggtGATCCTGCGTGCCAGA‑‑AAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGACGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCCCATTGACCGCGCCATCGACAGCCTCAGCGG‑CAAGTTGCTGGAAGAG‑TTTATTGTGGTGGTGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGc  <  2:436986/251‑1 (MQ=255)
                                                                                                                                                                                                                                      ggtggtGATCCTGCGATCCGGC‑‑AAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACCTCAGCGG‑CAAGTTGCTGGAAGAG‑TTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGc  >  2:148079/1‑251 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                            
GATGCTTCAAACCAGGAAGCGGGCGGTTCGTCGATCGAACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGC‑‑AAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACCTCAGCGG‑CAAGTTGCTGGAAGAG‑TTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGC  >  NC_000913/599344‑599824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑