Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913357,8900CA47.6% 1.5 / 11.9 21L267F (TTG→TTT) cynRDNA‑binding transcriptional dual regulator CynR
Reads supporting (aligned to +/- strand):  ref base C (10/1);  new base A (0/10);  total (10/11)
Fisher's exact test for biased strand distribution p-value = 3.40e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.07e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGT‑AGGCCTGATAAGCGTAGCGCATCAGGCAA‑TTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATT‑CATTTCC‑GCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCG‑CCGCAACAAAACC‑GCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAG‑CTTTAAGCCCGTCATGTTGTGTGGC‑AATCGCTGCTGGTAACAATGTGG‑AAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGAGCAGGACCAGTTTTTCAT  >  NC_000913/357645‑358128
                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                  
aaaTGCCTTCGCAGCGGCTGGCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCCTCTCCGGCCTACGTAGAGCCCTGAACTCGT‑AGGCCTGATAAGCGTAGCGCATCAGGCAA‑TTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATT‑CATTTCC‑GCCAACAACCGCGCATTTATCCCACGCCATGTGCCAAAATGCC‑CTCGCGGCGGCTGTCTGCCCGCTCTTTTTCCC‑CCGCGACAAAAc                                                                                                                                                                                                                                               >  2:15106/1‑251 (MQ=18)
      cTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGT‑AGGCCTGATAAGCGTAGCGCATCAGGCAA‑TTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATT‑CATTTCC‑GCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCG‑CCGCAACAAAACC‑GCCGt                                                                                                                                                                                                                                        >  1:500813/1‑251 (MQ=255)
                gCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGT‑AGGCCTGATAAGCGTAGCGCATCAGGCAA‑TTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATT‑CATTTCC‑GCCAACAACCGCGCCTTTATCCAACGCCATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCG‑CCGCAACAAAACC‑GCCGTTCTCTCCagt                                                                                                                                                                                                                              >  1:45132/1‑251 (MQ=255)
                              gTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCCGCCTACGTAGAGCACTGAACTCGT‑AGGCCTGATAAGCGTAGCGCATCAGGCAA‑TTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGCTT‑CATTTCC‑GCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCG‑CCGCAACAAAACC‑GCCGTTCTCTCCAGTCGTGGCgggggggg                                                                                                                                                                                                                >  1:456694/1‑247 (MQ=255)
                                aGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGT‑AGGCCTGATAAGCGTAGCGCATCAGGCAA‑TTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATT‑CATTTCC‑GCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCG‑CCGCAACAAAACC‑GCCGTTCTCTCCCGTAGTGGCggggggtagg                                                                                                                                                                                                              >  2:172958/1‑245 (MQ=255)
                                    tATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGT‑AGGCCTGATAAGCGTAGCGCATCAGGCAA‑TTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATT‑CATTTCC‑GCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCG‑CCGCAACAAAACC‑GCCGTTCTCTCCAGTAGTGGCggggggaggagaa                                                                                                                                                                                                           >  1:126954/1‑241 (MQ=255)
                                          ggATGCGGGGTGACCGCTTTACCCGGCCTACGTAGAGCAC‑GAACCCGTTGGGCATAAAAGGCGTGGCCCATC‑GGCAAATCCCCGCCGCAGACTTGTGTCGGCGGCT‑CCGTTGTT‑TATTTCCCGCAAACACCCGCGCATTTACCCAACGCAATTTG‑AAAAATGCCCTCCGCGGCGGCTGTCTGCCGGTTTTTTTCCCG‑CCGCAAAAAAACC‑GCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAG‑Cttt                                                                                                                                                                                                   <  1:265889/251‑1 (MQ=255)
                                                   gTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGT‑AGGCCTGATAAGCGTAGCGCATCAGGCAA‑TTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATT‑CATTTCC‑GCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCG‑CCGCAACAAAACC‑GCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGaaaaaaattttaacccctc                                                                                                                                                                                           >  1:84037/1‑235 (MQ=255)
                                                   gTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGT‑AGGCCTGATAAGCGTAGCGCATCAGGCAA‑TTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATT‑CATTTCC‑GCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCG‑CCGCAACAAAACC‑GCCGTTCTCTCCAGTAGTGGCGGGGCAGGAGaaaaagattttaatccagc                                                                                                                                                                                           >  1:154796/1‑235 (MQ=255)
                                                                                                                     gCAA‑TTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATT‑CATTTCC‑GCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCG‑CCGCAACAAAACC‑GCCGTTCTCTCCAGTAGTGGCGGGGGAAGAGAAAAAA‑CTTTAAAACCCTCCTGGTTTGTGGG‑GATCGCTGCTGGTGACCAAGTGG‑GAAGGGGAGAgcgggggaagagcgccaca                                                                                                                        >  1:592308/1‑235 (MQ=255)
                                                                                                                                 cgaagtccTGTGTCGGCGGCTACC‑TGATTTCATTCCC‑ACCAACAACCGCGCATTTACCCACCGCCATGTGCAAAAATGCC‑TTCGCGGCGGCTTTCTGCCAGTTTTTTTCCCG‑CCGCAACAAAACC‑GCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAG‑CTTTAAGCCCGTCATGTTGTGTGGC‑AATCGCTGCTGGTAACAATGTGG‑AAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAAtt                                                                                                            <  1:383058/245‑1 (MQ=255)
                                                                                                                                                               tatttccc‑GCCAACACCCGCGCATTTATCCACCGCCATGTGCAAAATCCCC‑TGCGCGGCGGCTGTCTGCCAGTTTTTTTCCCG‑CCGAAAAAAAACC‑GCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAG‑CTTTAAGCCCGTCATGTTGTGTGGC‑AATCGCTGCTGGTAACAATGTGG‑AAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGAt                                                                                <  1:15106/244‑1 (MQ=255)
                                                                                                                                                                                                 cccttgtgAAAAAATCCC‑TCCGCGGCGGCTGTCGGCCACTTATTTTCCCG‑CCGCAAAAAAACC‑GCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAG‑CTTTAAGCCCGTCATGTTGTGTGGC‑AATCGCTGCTGGTAACAATGTGG‑AAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTg                                              <  1:381084/247‑1 (MQ=255)
                                                                                                                                                                                                    aTGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCG‑CCGCAACAAAACC‑GCCGTTCTCTCCAGTAGTGGCGGGGCCAGAGAAAAAAATTTTAACCCCG‑CCTTTTGTTTGGGGAAAC‑CTGCTGGTGACAATGTGGGAAAGGGA‑GGGCGGCGGATCAGCTCCCGAACCCCGCCCATTTAGTTTGCCCCCAAGACCACCCGTGGGTGTAGCCCCCCCTtctccccgcagtggggaattttttt                                           >  1:168620/1‑230 (MQ=255)
                                                                                                                                                                                                        acaaaactccc‑TTCGCGGCGGTTTTCTCCCAGTTTTTTTCCCG‑CCGAAAAAAAACC‑GCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAG‑CTTTAAGCCCGTCATGTTGTGTGGC‑AATCGCTGCTGGTAACAATGTGG‑AAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGctctct                                        <  1:36370/241‑1 (MQ=255)
                                                                                                                                                                                                                     cgggggcgttttcctccacttttttttcCCG‑CCGCAAAAAACCC‑GCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAG‑CTTTAAGCCCGTCATGTTGTGTGGC‑AATCGCTGCTGGTAACAATGTGG‑AAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTcc                           <  2:125422/228‑1 (MQ=255)
                                                                                                                                                                                                                                  cgccatttttttttcCCG‑CC‑CAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAG‑CTTTAAGCCCGTCATGTTGTGTGGC‑AATCGCTGCTGGTAACAATGTGG‑AAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGAGCAgg               <  2:168620/240‑1 (MQ=255)
                                                                                                                                                                                                                                   cgCCAGTTATTTTCCCC‑CCGCAAAAAAACC‑GCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAG‑CTTTAAGCCCGTCATGTTGTGTGGC‑AATCGCTGCTGGTAACAATGTGG‑AAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGAGCAGGAc             <  1:461306/250‑1 (MQ=255)
                                                                                                                                                                                                                                           ttttttCCGGCGGCAAAAAAACC‑GCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAG‑CTTTAAGCCCGTCATGTTGTGTGGC‑AATCGCTGCTGGTAACAATGTGG‑AAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGAGCAGGACCAGttt       <  2:375209/249‑1 (MQ=255)
                                                                                                                                                                                                                                            tttttccccccgaaacaaAACC‑GCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAG‑CTTTAAGCCCGTCATGTTGTGTGGC‑AATCGCTGCTGGTAACAATGTGG‑AAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTTGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGAGCAGGACCAGtt        <  2:537488/235‑1 (MQ=255)
                                                                                                                                                                                                                                             ttttccccccgcAAAAAACCC‑GCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAG‑CTTTAAGCCCGTCATGTTGTGTGGC‑AATCGCTGCTGGTAACAATGTGG‑AAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGAGCAGGACCAGttttt     <  1:560608/242‑1 (MQ=255)
                                                                                                                                                                                                                                               tcccg‑ccgCAAAAAAACC‑GCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAG‑CTTTAAGCCCGTCATGTTGTGTGGC‑AATCGCTGCTGGTAACAATGTGG‑AAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGAGCAGGACCAGTTTTtcat  <  1:172958/248‑1 (MQ=255)
                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                  
AAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGT‑AGGCCTGATAAGCGTAGCGCATCAGGCAA‑TTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATT‑CATTTCC‑GCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCG‑CCGCAACAAAACC‑GCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAG‑CTTTAAGCCCGTCATGTTGTGTGGC‑AATCGCTGCTGGTAACAATGTGG‑AAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGAGCAGGACCAGTTTTTCAT  >  NC_000913/357645‑358128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑