| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 354,288 | 0 | T | G | 32.3% | 131.9 / 24.2 | 65 | V528G (GTG→GGG) | prpE | propionyl‑CoA synthetase |
| Reads supporting (aligned to +/- strand): ref base T (2/42); new base G (21/0); total (23/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.17e-15 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCGCCGGGGTGTATTCAGACCATCTGGGGCGACGACGGCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGTGGACAGCCAGATTGG‑CAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATC > NC_000913/354044‑354533 | gtttgaagcggtATTCAGACCATCTGGGGCGACGACGGCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGaaa < 1:580239/239‑1 (MQ=255) ccgGGGTGTATTCAGACCATCTGGGGCGACGACGGCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATgc < 2:566618/251‑1 (MQ=255) cgGGGTGTATTCAGACCATCTGGGGCGACGACGGCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATgcg < 1:501810/251‑1 (MQ=255) aTTCAGACCCTCTGGGGCGACGACGGCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGG‑GTGGGGGGAAAggaggcccggaagg > 1:409660/1‑238 (MQ=255) aGACCATCTGGGGCGACGACGGCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGGTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGGGGaaggaggggcgcgagggg > 2:581719/1‑233 (MQ=255) gACCATCGGGGGCGTCGACGGCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAgg < 2:626914/251‑1 (MQ=255) cgacgGCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGCGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTg < 2:5607/251‑1 (MQ=255) gCCGGTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCAtt < 2:263938/251‑1 (MQ=255) ccGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTc < 2:258481/251‑1 (MQ=255) tgtgAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTTGGGGGAAAGATGCGCTGGAAGGGCGggtggggggggggtttttgttttcgaa > 2:39911/1‑227 (MQ=255) tACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACa < 2:587350/250‑1 (MQ=255) aCTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAg < 2:77846/250‑1 (MQ=255) aCTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGt < 1:602831/251‑1 (MQ=255) aCTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGGGGAAAGATGGGCTGAAAGGGCAGGGGGCGG‑GGGCGGTTGGCATTCCGAAagagaggggcagg > 1:51886/1‑244 (MQ=255) cTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGGGGGAAGATGGGCTGAAAGGGCAGGTGGGGG‑TGGCGGTTGTCATTCCGAAAGAGAGCGACAGTc > 1:290317/1‑251 (MQ=255) ttCCCGCCCGGTGTACGCCACCTTTGACTGGGGTATCCGTTACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGt < 2:503326/250‑1 (MQ=255) ccgGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCGGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgc < 1:283238/251‑1 (MQ=255) gtgtACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGAc < 2:588473/225‑1 (MQ=255) aCGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTc < 2:404589/250‑1 (MQ=255) aCGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑g < 2:551770/251‑1 (MQ=255) cACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGGGGAAGATGGGCCGGAAGGGGAGGGGGGGG‑GGGGGGTTGTTATTCCGAAAGAGAGAGACAAGCTGGAAGATCGTGGTGTgggggactccca > 1:6918/1‑239 (MQ=255) ccTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACGTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAagag < 2:395801/251‑1 (MQ=255) ttGACGGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAgaga < 1:610425/249‑1 (MQ=255) aCGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCtggtg < 1:559688/251‑1 (MQ=255) cGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCtggtgg < 2:155943/251‑1 (MQ=255) ggTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGTGGACAGCCa < 2:562882/251‑1 (MQ=255) ggTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGGGGAGAAATGCGCCGGAAGGGCAGGTGGGGG‑GGGCGGTTGTTAATCCGAAAGAGAGGGGAAGGAGGGGAGGTCGTGATTGGGGGCACCC‑GCAAGAGAAGG‑CGATT‑A‑TGgcgggggggggccgcac > 2:432768/1‑237 (MQ=255) ttATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGGGG‑TGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGGGCACTC‑GCAAGAGAAGG‑CGATTTA‑TG‑CGCTGGTGGGCAGCCAgg > 1:438714/1‑250 (MQ=255) aTCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGTGGACAGCCAGAtt < 2:588491/251‑1 (MQ=255) cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGTGGACAGCCAGATTg < 1:206427/250‑1 (MQ=255) aCTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGTGGACAGCCAGATTGG‑c < 1:134904/251‑1 (MQ=255) cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGTGGACAGCCAGATTGG‑CAACTTTg < 1:39911/248‑1 (MQ=255) cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGTGGACAGCCAGATTGG‑CAACTTTGgcc < 2:472697/251‑1 (MQ=255) gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGTGGACAGCCAGATTGG‑CAACTTTGGCCGCCCg < 1:328535/251‑1 (MQ=255) cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGTGGACAGCCAGATTGG‑CAACTTTGGCCGCCCgg < 1:471514/251‑1 (MQ=255) aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGGGGGAAGATGCGCTGGAAGGGGCGGGGGGGG‑TGGCGGTTGTCATTTCGAAAGAGAGCGACAGTCTGGGAGATCGTGATGTGGCGCACTC‑GCAAGAGAAAG‑CGGTT‑A‑TTGCGCTGGTGGACAGCCCGATTTG‑CCACTTTTGGCGCCCGGCGCAc > 1:604976/1‑251 (MQ=255) cGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGTGGACAGCCAGATTGG‑CAACTTTGGCCGCCCGGCGCACg < 2:484471/251‑1 (MQ=255) gATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGG‑GTGGGGTGAAAACGGCGCTGAAAAGGCAGGTGGGGG‑GGACGTTTTTCATTCCGAAAGAAAGCGACAGACTGGAAAATCATGATGTGGCGCACTCTGGAA‑AGAAAG‑CGATT‑AATGG‑GCTGGTGGCCAACCCGATTGG‑CGACTTTTGCCCCCCGGCGCCCGt > 1:167004/1‑251 (MQ=255) aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGGGAAAGAAGCGCTGAAAGGGGAGGTGGGGG‑GGGCGTTTGTCCTTTCGAAAGAGAGGGGCAGTCTGGAAGAACGTGATGTGGGGCCCTC‑GCAAGAGAAGG‑GGGTT‑T‑TGGCGCTGGTGGACAGCCCGATTGG‑CAAATTTTGGCGCCCCgcgcccgcc > 2:417511/1‑246 (MQ=255) tGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGGGGGAAGATGGGCTGAAAGGGGAGGGGGCGG‑GGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGATGTGGGGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGGGGACAGCCAGATTGG‑CAACTTTGGCCGCCCGGCGCACGTCt > 1:518639/1‑251 (MQ=255) aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGTGGACAGCCAGATTGG‑CAACTTTGGCCGCCCGGCGCACGTCTgg < 2:409660/250‑1 (MQ=255) aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGGGGAAAATGCGCTGGAAGGGGAGGGGGCGG‑GGGCGTTTGTCATTTCGAAAGAGAGCGACAGGCTGGGAGATCGTGGTGTGGCGCACTC‑GCAAGAGAAGG‑GGATT‑A‑TTGCGCTGGTGGACAGCCAGAATGG‑CAACTTTGGCCGCCCGgcgccagcctggtt > 1:56718/1‑241 (MQ=255) aaCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGCGG‑GGGCGTTTGTCATTCCGAAAGAGAGGGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGGGCTGGTGGGCAGCCAGACTGG‑CAACTTTTGCCGCCCCGCGCCCGTCTgggtttt > 1:113026/1‑246 (MQ=255) cGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGCGG‑GGGCGGTTGTCATTCCGAAAGAGAGCGACCGGCTGGAAGATCGGGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGGGGGCAGCCAGATTGG‑CAACTTTGGCCGCCCCGCGCACGTCTGGGTTGtct > 1:529543/1‑251 (MQ=255) tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCAGTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGTGGACAGCCAGATTGG‑CAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCg < 2:235870/250‑1 (MQ=255) ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGGGGAAAGATGGGCCGAAAAGGGAGGGGGCGG‑GGGGGGTTTTCATTCCGAAAGAGAGCGAAAGTCTGGGAGATCGGGAGGCGGCGCACTC‑GCAAGAGAAGG‑GGGAT‑A‑TGGGGCTGGGGGGCAGCCCGATTGGGCACTTTTGGCC‑CCCGGCGCACGTCTGGGTTGTGTCGct > 1:630363/1‑249 (MQ=255) gggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGGGGGAAGATGGGCTGGAAGGGCAGGTGGGGGCGGGGGTTTGTC‑TTTCGAAAGAGAGCGGCAGGCTGGGAGATCGTGGTGTGGGGCACCC‑CCAAGAGAAGGGCG‑TT‑A‑TGGCGCTGGTGGAGAGCCAGCTTCG‑GAACTTTGGCCGCCCCGCGCACGGCTgggtcgcatccccact > 2:523918/1‑237 (MQ=255) aTCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGTGGACAGCCAGATTGG‑CAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCa < 2:102999/251‑1 (MQ=255) ggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGGGGAAGATGCGCTGAAAGGGCCGGTGGCGG‑GGGCGGTTGTCATTCCGAAAAAGAGCGGCAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGGGGGCAGCCCGATTGG‑CAACTTTGGCCGCCCGGCGCACCTCTGGTTTGTCTCGCAATTGCCAAAAAagcgg > 1:280613/1‑250 (MQ=255) cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGTGGACAGCCAGATTGG‑CAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCgg < 2:438714/251‑1 (MQ=255) gcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGTGGACAGCCAGATTGG‑CAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGa < 1:432768/251‑1 (MQ=255) cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGGGAAAAATGCGCTGAAAGGGCAGGTGGGGG‑GGGCGTTTGTGATTCCGCAAAAGGGCGACAGGATGGAAGATCGGGATGTGGCGCACTC‑GCCAGAAAAGG‑CGGGT‑A‑TGGCGCTGGGGGGCAGCCCGATTTG‑CAACTTTGGCCGCCCGGCGCCCGTCTGGGTTGTCTCGCAAATGCCAAAAAAGCGAGCCggg > 1:406317/1‑249 (MQ=255) cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGG‑GTGGGGGGAAAGAAGCGCTGAAAGGGCGGGTGGGGG‑GGGCGGTTGTCATTCCGGAAGAGAGCGACAGTCTGGAAGATCGGGGTGGGGGGCACTC‑GCCAGAGAAGG‑CGATT‑A‑TGGCGCTGGTGGAAAGCCCGATTGG‑CAACATTGGCCGCCCGGCGCACGGCTGGTTTGTCTCGCAATTGCCAAAAACACGATCCGGa > 2:565033/1‑250 (MQ=255) gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGTGGACAGCCAGATTGG‑CAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAAtgc < 1:88698/250‑1 (MQ=255) gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGG‑GTGGGGGGGAAGAAGCGGTGAAAGGGCAGGGGGGGG‑GGGCGGTTGTCATTCCGAAAGAGAGCGACAGGCTGGGAGATCGTGGTGGGGGGCACTC‑GCAAGAGAAGG‑GGAGT‑A‑AGGCGCTGGTGGACAGCCAGAATGG‑CAACTTTTGCCGCCCGGCGCACGGCTGGGTTTTCTCGCCAATGCCAAAAACCCGATTCGGaaaaaagc > 2:88698/1‑247 (MQ=255) agagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGTGGACAGCCAGATTGG‑CAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTgcgc < 1:414522/251‑1 (MQ=255) aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGG‑GGGCGGTTGTCATTCCGAAAGAGAGCGACAGGCTGGGAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGGGGACAGCCAGATTGG‑CAACTTTGGGCGCCCGGCGCACGTCTGGGTTGTCTCGCAATTGCCAAAAAAGCCATCCGGAAAAATGCTGCGCCGCAc > 1:414102/1‑251 (MQ=255) ccAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGTGGACAGCCAGATTGG‑CAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGAt < 1:135637/250‑1 (MQ=255) aGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGTGGACAGCCAGATTGG‑CAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCa < 1:606018/251‑1 (MQ=255) gTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑GGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGTGGACAGCCAGATTGG‑CAACTTTTGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTTCCAAAAACGCGATCCGGGAAAATGCTGCGCCGCACGATCCAGGCGATTTGCg > 1:539546/1‑250 (MQ=255) gCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGTGGACAGCCAGATTGG‑CAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAg < 1:136993/250‑1 (MQ=255) ggCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGTGGACAGCCAGATTGG‑CAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGAtn < 2:290317/251‑2 (MQ=255) ggtgg‑tTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGTCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGTGGACAGCCAGATTGG‑CAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTg < 2:630363/251‑1 (MQ=255) ggtgg‑tTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGTGGACAGCCAGATTGG‑CAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTg < 1:523918/251‑1 (MQ=255) ggggTGAAAGATGAGCTGGAAGGGCAGGTGGCGG‑TGGCGTTGGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAGCGTGATGTGACGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGTGGACAGCCAGATTGG‑CAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATc < 1:540537/250‑1 (MQ=255) | CCGCCGGGGTGTATTCAGACCATCTGGGGCGACGACGGCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGG‑TGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTC‑GCAAGAGAAGG‑CGATT‑A‑TGGCGCTGGTGGACAGCCAGATTGG‑CAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATC > NC_000913/354044‑354533 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |