Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,123,930	A→T	V176E (GTA→GAA)	cytR ←	DNA‑binding transcriptional repressor CytR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,123,930	0	A	T	100.0%	248.2 / NA	63	V176E (GTA→GAA)	cytR	DNA‑binding transcriptional repressor CytR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (26/37);  total (26/37)

GAGCGCCATCACATCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTACTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGAT  >  NC_000913/4123683‑4124173                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       gAGCGCCATCACATCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTg                                                                                                                                                                                                                                                     <  1:76300/251‑1 (MQ=255) gAGCGCCATCACATCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGAAGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATAAAGCCAATCCGTTTATGCCCTTGCTCCTATAAATAATTTTCTg                                                                                                                                                                                                                                                     >  1:37104/1‑251 (MQ=255)  aGCGCCATCACATCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCCTCTCTTCGGGACCGGCTATACCGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGc                                                                                                                                                                                                                                                    >  1:319077/1‑251 (MQ=255)                       cAGAAGACAGCAGTAGGCGGTTGTTGAAGATCAAGCAGCTGCTGCATCGCTTTGCTGCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGAtt                                                                                                                                                                                                                               <  2:189202/251‑1 (MQ=255)                          aagaCAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTc                                                                                                                                                                                                                            >  1:248988/1‑251 (MQ=255)                          aagaCAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGGGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCCTGCTCATATAAATAATTTTTTGCATCAAATGCGGCGGGCAAATTGtt                                                                                                                                                                                                                            >  2:293012/1‑250 (MQ=255)                                 cagTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCCGATTGTCGATAT‑g                                                                                                                                                                                                                     >  1:191214/1‑250 (MQ=255)                                        cGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAg                                                                                                                                                                                                             <  1:170956/251‑1 (MQ=255)                                               ggAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGcagct                                                                                                                                                                                                       <  2:127784/250‑1 (MQ=255)                                                      cAAGCAGCTGCTGCATCGCTCTGCTCCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGcagctccagctc                                                                                                                                                                                                <  2:345201/250‑1 (MQ=255)                                                           agcTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCCGATTTTCCATAT‑GAAATGTAGGGAGCTCCCACTCC‑ggggg                                                                                                                                                                                         >  2:368272/1‑248 (MQ=255)                                                            gctgctGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCa                                                                                                                                                                                        <  1:198743/251‑1 (MQ=255)                                                                  gcATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAAttc                                                                                                                                                                                   <  2:117560/250‑1 (MQ=255)                                                                          ttGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGc‑c                                                                                                                                                                           <  1:141003/249‑1 (MQ=255)                                                                               tCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCAc                                                                                                                                                                      <  2:129402/249‑1 (MQ=255)                                                                                ccGGCTTCGAAGGTGACGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGCGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCCTATAAATAATTTTCTGCATCAAATGCGGCGGTCCGATTGTTGATATTAAACTGTGGGCA‑CTCCAGCCCCCGGTGCAATttcgtctccccttccat                                                                                                                                                                     >  2:415854/1‑239 (MQ=255)                                                                                 cGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATc                                                                                                                                                                  <  1:3507/251‑1 (MQ=255)                                                                                  ggCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCCGCTCC‑GGGGCAAATTTGTTCGC‑CATCCCCATcc                                                                                                                                                                 >  1:488467/1‑250 (MQ=255)                                                                                      tCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCCGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGGGCAAATTCGTTCGC‑CATCACCATcggcgg                                                                                                                                                             >  1:567536/1‑251 (MQ=255)                                                                                       cGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCggcggc                                                                                                                                                            <  2:473381/251‑1 (MQ=255)                                                                                               aaGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACg                                                                                                                                                    <  1:385437/251‑1 (MQ=255)                                                                                                aGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATc                                                                                                                                                                  <  2:420522/236‑1 (MQ=255)                                                                                                aGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCCATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTTTCGGTTT‑GAACTGTAGGCAACTCCCGCTCC‑CGTGCAAATTCGCTTGC‑CATCAccctc                                                                                                                                                                  >  1:420522/1‑233 (MQ=255)                                                                                                    gCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGtt                                                                                                                                               <  2:560111/251‑1 (MQ=255)                                                                                                                    tGCGGATCAACCATAAGGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCGCTTCGGGACCGGCTATACAGCCTATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCCAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCAt                                                                                                                                <  2:102603/250‑1 (MQ=255)                                                                                                                       ggATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTTTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCCGTTCCTCAATGCTGGCATCaa                                                                                                                             >  1:118437/1‑250 (MQ=255)                                                                                                                        gATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑TAACTTTAGGGAACTCCAGCTCC‑GGTGGAAATTCGTTCGC‑CATCACCATCCGCGGCAGATTTCGCTGTTTCTCCAAGCTGGCATCaaa                                                                                                                            >  1:356628/1‑250 (MQ=255)                                                                                                                                aTAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCCGATTGTCGATAT‑GAACTGTa                                                                                                                                                                                                              >  1:206921/1‑162 (MQ=255)                                                                                                                                aTAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTa                                                                                                                                                                                                              <  2:206921/162‑1 (MQ=255)                                                                                                                                aTAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCt                                                                                                                   <  1:20003/251‑1 (MQ=255)                                                                                                                                 tAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGGTAA‑TAAATGGAGGCAGCTCCAGCTCC‑GGGGCAAAATCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCCCAATGCTGGCCTCAAAAGGCAGCCtt                                                                                                                  >  1:359080/1‑251 (MQ=255)                                                                                                                                   aTGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGa                                                                                                                <  2:118437/251‑1 (MQ=255)                                                                                                                                    tGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATcggc                                                                                                                                                               <  1:205185/203‑1 (MQ=255)                                                                                                                                    tGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGCAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCCGATTGTCGGTAT‑TAACTGTAGGCAGCTCCAGCTCC‑GGGGCAAATTTGTTCGC‑CATCACCATcggc                                                                                                                                                               >  2:205185/1‑203 (MQ=255)                                                                                                                                         cAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAg                                                                                                          <  1:293012/251‑1 (MQ=255)                                                                                                                                                 cAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCaacaacn                                                                                                  <  2:282343/251‑2 (MQ=255)                                                                                                                                                               aGCCTTGCAGGAGGGCGTGACACAGCGGCATCTCTTCGGGACCGGCTATACACCCAATCCCTCTATGCCCTTCCTCATATAAATAATTTTCTGCATCAAATTCGGCGGTCCGATTGTCCACAT‑AAACTTTAGGCAGCTCCAGCTTC‑GGGGCCAATTCGTTCACACATC‑CCCTCGGCGGCAGACTACCCCGTTCCTCAATGCTGCTCTCAAAAGGCAGCCCTTAACACAGCACCAAAATGccctccaattct                                                                                    >  2:525365/1‑241 (MQ=255)                                                                                                                                                                       aGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGtgat                                                                            >  1:91357/1‑251 (MQ=255)                                                                                                                                                                                 gACACATCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATc                                                                   <  1:331161/250‑1 (MQ=255)                                                                                                                                                                                   cacaGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGAt                                                                <  1:415854/251‑1 (MQ=255)                                                                                                                                                                                    acaGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCCGCCTTGAACCCAGCCACAACATGCCATCAATTTGCTTGGtgatg                                                                           >  2:39497/1‑239 (MQ=255)                                                                                                                                                                                    acaGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGtgatg                                                                           <  1:39497/239‑1 (MQ=255)                                                                                                                                                                                      aGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATaaa                                                             <  1:368272/251‑1 (MQ=255)                                                                                                                                                                                         ggCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGt                                                          <  2:311633/251‑1 (MQ=255)                                                                                                                                                                                                ctTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGtttttt                                                     <  1:429193/249‑1 (MQ=255)                                                                                                                                                                                                  tCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACTCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTcn                                                   <  2:271942/249‑2 (MQ=255)                                                                                                                                                                                                     ggACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAAGCGATAAAGGTTTTTTCctgctg                                              <  1:150960/251‑1 (MQ=255)                                                                                                                                                                                                      gACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATACTTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATACGTTCGC‑CATCACCAACGGATGCCGATTACGCTGTTCCTTAATTGTGGCATCAAAACGCAGCCTTGAACCCAACAATAACATGCCATCAATTTGCTTGGTGATGATCAAATAGATAAAGGATTTTTCctgctgg                                             >  2:68903/1‑250 (MQ=255)                                                                                                                                                                                                      gACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGGGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTTCTTGGTGATGATCAAATCGATAAAGGTTTTTTCctgctgt                                             >  2:159027/1‑250 (MQ=255)                                                                                                                                                                                                       aCCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGAt                                            <  1:85825/251‑1 (MQ=255)                                                                                                                                                                                                         cGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGAttt                                          >  1:370480/1‑251 (MQ=255)                                                                                                                                                                                                                   gCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGcac                                <  2:203463/251‑1 (MQ=255)                                                                                                                                                                                                                      aaTCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAAc                                                                                                                           >  2:147446/1‑157 (MQ=255)                                                                                                                                                                                                                      aaTCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAAc                                                                                                                           <  1:147446/157‑1 (MQ=255)                                                                                                                                                                                                                       aTCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCAcc                                                                                                                                                                     >  2:372875/1‑114 (MQ=255)                                                                                                                                                                                                                       aTCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCAcc                                                                                                                                                                     <  1:372875/114‑1 (MQ=255)                                                                                                                                                                                                                          cGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGGTTTTTCCTGCTGATTTTGATGCGCACAGTCGcc                         >  1:94102/1‑251 (MQ=255)                                                                                                                                                                                                                                 gCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGc                  <  2:488467/251‑1 (MQ=255)                                                                                                                                                                                                                                        ctcgtataAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCAcc               >  1:592387/5‑247 (MQ=255)                                                                                                                                                                                                                                         tCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGATCCCGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGat           >  1:594691/1‑250 (MQ=255)                                                                                                                                                                                                                                           gtataAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATc        <  2:592387/250‑1 (MQ=255)                                                                                                                                                                                                                                             ataaataaTTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATcc       <  1:310374/250‑1 (MQ=255)                                                                                                                                                                                                                                                 ataatTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGAt  <  1:320501/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       GAGCGCCATCACATCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTACTGCATCAAATGCGGCGGTCAGATTGTCGATAT‑GAACTGTAGGCAGCTCCAGCTCC‑GGTGCAAATTCGTTCGC‑CATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGAT  >  NC_000913/4123683‑4124173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑