Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,151,6300CA54.5% 5.8 / 16.3 11A149E (GCA→GAA) yegJDUF2314 domain‑containing protein YegJ
Reads supporting (aligned to +/- strand):  ref base C (1/4);  new base A (0/6);  total (1/10)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CCGAGCATTTCTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAA  >  NC_000913/2151402‑2151863
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                         
ccGAGCATTTCTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGcc                                                                                                                                                                                                                     <  1:130706/251‑1 (MQ=255)
ccGAGCATTTCTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGcc                                                                                                                                                                                                                     <  1:66261/251‑1 (MQ=255)
 cGAGCATTTCTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCg                                                                                                                                                                                                                    <  2:35907/251‑1 (MQ=255)
                                   aaCGGCATGTTGATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGATCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAAttg                                                                                                                                                                                   <  2:344022/250‑1 (MQ=255)
                                                               atcaCCCTGTTTTTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATAAAAAAAAAAGATTTTTCAGACTGGATGTATACGCGCGGGGAAAAAATTTCCGGCGGTTATACCTTTGATCCTTTATGGGTCCCCTATCCAAAAAAAGAAGCCGACGAGCTAAGAGAAAAATTATTTCGCTAATCAGCCGTTATATTTATTTTTTAAACTGGCGTTTAATTTTTTGTCTTCAACCGCCATGAGAAactcact                                                                                                                                                      <  1:46453/248‑1 (MQ=255)
                                                                                                                     aagaagATATTTCAGTCGGGATGTTGCCGCGGGGAGAAAAAATTTAGGGGGTTTATACCTTTGATCTTTTATTGGCCCCCTTACAAAAAGAAGAAGCCGACGAAAAAAGAGAAAAATTAGTTCCTTAACCAGCCGTTATATTTATTTTTAAAACGGGGGGTTAATTTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTAccg                                                                                                <  2:586140/251‑1 (MQ=255)
                                                                                                                         aatattttCAGACTGGTTGTATCCGGGCGGGGAAAAATTTTCCGGGGGTTATACAATTGTTCTTTTATTGTCCCCCTACCAAAAAGAAGAAGCCGACGAGTTAAGACAAAAATTATTTCTTTATTCAGCTGTTATATTTATTGTTTAACCTGGCGTTGGAATTTTTGTCTTCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCgg                                                                                            <  1:111004/246‑1 (MQ=255)
                                                                                                                                                                      ggtttATCCCATTGATCCTTTTTTGGTCACCTACCAAAAAGAAGAGGCCGACGAGTAAAGAGAAAAATTAGTTCGCTAATCAGCCTTTATATTTATTGTTTAAACGGGCGGTTGATTTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAgttg                                               <  1:602308/248‑1 (MQ=255)
                                                                                                                                                                                        tttATTGTTCCCCTATCAAAAAGAAGAACCCGACGAGCAAAGAGAAAAATTATTTCCCTAATCACCCGTTATTTTTTTTGTTAAACCGGGGGGTTGATTTTTTTTCTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTgcc                               <  1:544458/249‑1 (MQ=255)
                                                                                                                                                                                                                   agccgaggagaaaaGAGAAAAATTATTTCGCTAACCAGCTGTTTTATTTTTTTTTAAAACGGGGGTTTGATTTTTTTTCTCCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTaaagaa  <  1:498054/239‑1 (MQ=255)
                                                                                                                                                                                                                            gCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTTAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTAc                                                                                                            >  1:4074/1‑136 (MQ=255)
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                         
CCGAGCATTTCTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAA  >  NC_000913/2151402‑2151863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑