Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,905,761	G→A	G25D (GGT→GAT)	mntP →	Mn(2(+)) exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,905,761	0	G	A	100.0%	253.3 / NA	65	G25D (GGT→GAT)	mntP	Mn(2(+)) exporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/35);  total (30/35)

AAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCGTCGACACGGTTTCT  >  NC_000913/1905523‑1906003                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   aaaaCGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGTTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                                                                                                                                                                                                                         >  1:112328/1‑250 (MQ=255)     cGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATaaa                                                                                                                                                                                                                                    <  1:640529/251‑1 (MQ=255)           aCGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGaaa                                                                                                                                                                                                                              <  2:758429/251‑1 (MQ=255)                 ggACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCt                                                                                                                                                                                                                        <  2:382170/251‑1 (MQ=255)                             ttCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCg                                                                                                                                                                                                             <  2:470731/250‑1 (MQ=255)                                 gtcagGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCg                                                                                                                                                                                                        >  1:106928/1‑251 (MQ=255)                                 gtcagGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACACCGGTCAGGACATTGTCCTGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCg                                                                                                                                                                                                        >  1:764373/1‑251 (MQ=255)                                    agGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTTCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGcc                                                                                                                                                                                                     >  2:476938/1‑251 (MQ=255)                                    agGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGAAAATATGCCACCCTCCATAAACCGAACTTTTCTGAAGCATTGCGAACCGGcc                                                                                                                                                                                                     >  2:621640/1‑251 (MQ=255)                                      gCGAGACCATATGCACATCAATCGATATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCCGGACATTGTCATGAATATCAATGCTACTGTTCTTCTTGCGTTTGGTATGTCGTTGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAACTTTTCTGAAGCATTGCAAACCGGCCtt                                                                                                                                                                                                   >  2:137926/1‑251 (MQ=255)                                       cGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTa                                                                                                                                                                                                  <  1:357958/251‑1 (MQ=255)                                        gagaCCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTAt                                                                                                                                                                                                 >  1:656270/1‑251 (MQ=255)                                        gagaCCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTAt                                                                                                                                                                                                 <  1:536844/251‑1 (MQ=255)                                             cATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTACAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTg                                                                                                                                                                                            >  1:581434/1‑251 (MQ=255)                                                atGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGTCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGAGGATGTGTTTTATGGATACCCCGTTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTg                                                                                                                                                                                         <  1:137926/251‑1 (MQ=255)                                                  gCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCCTGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGcc                                                                                                                                                                                       <  2:88418/251‑1 (MQ=255)                                                      aTCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCg                                                                                                                                                                                   >  2:250996/1‑251 (MQ=255)                                                      aTCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGGTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCg                                                                                                                                                                                   >  1:763715/1‑251 (MQ=255)                                                       tCAATCGCTATGACTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGCATACCCCGGTCAGGACATTGGCATGAATCTCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTTGTGCCGTCgt                                                                                                                                                                                  >  2:776665/1‑250 (MQ=255)                                                            cGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAAccc                                                                                                                                                                             <  1:561049/251‑1 (MQ=255)                                                                aTGCCTGCATGAACGCGAAATCCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGa                                                                                                                                                                          <  2:744802/250‑1 (MQ=255)                                                                   ccTGCATGAACGCGAAAACCATCGCGGCTTGGTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGAcgcc                                                                                                                                                                      <  2:445328/251‑1 (MQ=255)                                                                   ccTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGTGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGAcgcc                                                                                                                                                                      <  1:355752/251‑1 (MQ=255)                                                                    cTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGAcgccg                                                                                                                                                                     >  1:297807/1‑251 (MQ=255)                                                                      gCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGGACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCt                                                                                                                                                                   >  1:390940/1‑251 (MQ=255)                                                                      gCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCt                                                                                                                                                                   <  1:449664/251‑1 (MQ=255)                                                                        aTGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTg                                                                                                                                                                  >  2:773496/1‑250 (MQ=255)                                                                                       aTCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATgg                                                                                                                                                  <  2:428707/251‑1 (MQ=255)                                                                                         cACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGc                                                                                                                                                >  2:640166/1‑251 (MQ=255)                                                                                            ggCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATg                                                                                                                                             <  1:414131/251‑1 (MQ=255)                                                                                               ttgttgTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGtt                                                                                                                                           >  1:45366/1‑250 (MQ=255)                                                                                                   tgttCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTagcc                                                                                                                                       <  1:325223/250‑1 (MQ=255)                                                                                                          gTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGATGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGtt                                                                                                                               >  2:728213/1‑251 (MQ=255)                                                                                                            aTGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGttt                                                                                                                              <  2:106928/250‑1 (MQ=255)                                                                                                                ttGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACAGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCt                                                                                                                         >  2:138909/2‑251 (MQ=255)                                                                                                                aTGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCt                                                                                                                         <  1:250996/251‑1 (MQ=255)                                                                                                                  gTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCtt                                                                                                                        <  1:444432/250‑1 (MQ=255)                                                                                                                       cTGGGGGCGATGCTGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGAATGGCGATGGATGCATTTGATGCATCAATCGGTAAAGATGCCACCCTCAATAAACCGAAGTTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCGGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGGCCTTGAATgg                                                                                                                  >  2:764815/1‑251 (MQ=255)                                                                                                                               gatgatGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTg                                                                                                          <  1:111115/251‑1 (MQ=255)                                                                                                                                atgatgTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTgg                                                                                                         <  1:476938/251‑1 (MQ=255)                                                                                                                                  gatgTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGAt                                                                                                       >  1:742752/1‑251 (MQ=255)                                                                                                                                     gtgtTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGc                                                                                                    <  1:280887/251‑1 (MQ=255)                                                                                                                                       gtTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGt                                                                                                                                                                                     >  1:330968/1‑168 (MQ=255)                                                                                                                                       gtTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGt                                                                                                                                                                                     <  2:330968/168‑1 (MQ=255)                                                                                                                                            aTGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTtgt                                                                                              <  2:787061/250‑1 (MQ=255)                                                                                                                                             tGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCTTGTTCGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTTtgc                                                                                            >  2:116497/1‑251 (MQ=255)                                                                                                                                              ggATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGtgct                                                                                           >  2:649091/1‑251 (MQ=255)                                                                                                                                                       ggTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATAtt                                                                                  >  2:126072/1‑251 (MQ=255)                                                                                                                                                       ggTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATAtt                                                                                  >  1:52374/1‑251 (MQ=255)                                                                                                                                                        gTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTc                                                                                 >  1:185174/1‑251 (MQ=255)                                                                                                                                                           aGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTc                                                                              >  1:143970/1‑251 (MQ=255)                                                                                                                                                                aTTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTcggc                                                                           <  2:432814/249‑1 (MQ=255)                                                                                                                                                                aTTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTcggc                                                                           >  1:432814/1‑249 (MQ=255)                                                                                                                                                                         aaTATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCCCTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGat                                                                <  1:764815/251‑1 (MQ=255)                                                                                                                                                                          atatCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGAc                                                                                                                                                                         >  1:104137/1‑145 (MQ=255)                                                                                                                                                                          atatCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGAc                                                                                                                                                                         <  2:104137/145‑1 (MQ=255)                                                                                                                                                                            atctcTGCTACTGTTCTTCTTGCGGTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCCCCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAGCCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGatta                                                              <  2:297807/246‑1 (MQ=255)                                                                                                                                                                            atCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCATCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGattat                                                             <  1:267141/251‑1 (MQ=255)                                                                                                                                                                                  gCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAg                                                         <  1:640166/249‑1 (MQ=255)                                                                                                                                                                                     aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTGATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGttt                                                    <  2:656270/251‑1 (MQ=255)                                                                                                                                                                                                 gCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAga                                        <  1:728213/251‑1 (MQ=255)                                                                                                                                                                                                   gTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAgatg                                      <  1:621640/251‑1 (MQ=255)                                                                                                                                                                                                    tttGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAggtg                                      >  1:122574/1‑247 (MQ=255)                                                                                                                                                                                                               gatggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGatgaagatgaag                          <  1:326583/251‑1 (MQ=255)                                                                                                                                                                                                                                       cGGTAAAGATGCCCCCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCGTCGACACGGTTTCt  <  2:45366/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   AAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCGTCGACACGGTTTCT  >  NC_000913/1905523‑1906003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑