Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,665,702	0	C	A	29.2%	90.1 / 14.5	49	A37S (GCG→TCG)	gadX	DNA‑binding transcriptional dual regulator GadX
Reads supporting (aligned to +/- strand):  ref base C (30/4);  new base A (0/14);  total (30/19)
Fisher's exact test for biased strand distribution p-value = 6.34e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGC‑GCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTC‑CCTT‑GACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGTTCTACGGGCAGGATGAC  >  NC_000913/3665452‑3665950                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                           gCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGC‑GCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTC‑CCTT‑GACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGc                                                                                                                                                                                                                                                            >  2:641516/1‑251 (MQ=255)      gATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGC‑GCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTC‑CCTT‑GACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCaaaa                                                                                                                                                                                                                                                        >  1:518505/1‑250 (MQ=255)          aaTGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGC‑GCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTC‑CCTT‑GACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAAcc                                                                                                                                                                                                                                                     >  2:776263/1‑249 (MQ=255)           aTGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGC‑GCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTC‑CCTT‑GACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑gg                                                                                                                                                                                                                                                >  2:555652/1‑251 (MQ=255)                     aCGTAGCATTACTGCATAGGGATGATTCTTATTCGGCGATAGTTGC‑GCACCTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAAATAAGAAACTTTTTTATGTGCATGAAGTTTTAATGCCTCCTCCTTGAGCATCGGGAAAAATAATGAAGGCGTGTC‑CCTT‑GACACGAATAAAAATTTTTCAACACTTTTATCTCCCCGAATTTGGCT‑TTCATCCGCAAAATAC‑A‑GGGCACCGCCAt                                                                                                                                                                                                                                      <  2:529526/251‑1 (MQ=255)                      cGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGC‑GCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTC‑CCTT‑GACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑GGTCACCGCCAtt                                                                                                                                                                                                                                     >  1:484377/1‑251 (MQ=255)                              ttCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGC‑GCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTC‑CCTT‑GACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGc                                                                                                                                                                                                                             >  2:197171/1‑251 (MQ=255)                                             atttttATTCGGCGATA‑TTGCCGCACCTTCCGAAAAAACAGAAATTTCCCTCATACAGTGATGAACGAGTAAGGAAGAAATTTTTTGATTGGCAGGAAGTTTTATTCCCCCCGCTTGGAGCATCGGGAAAAAAAAAGAAGGGTTCTC‑CTTA‑GACACGAATAAAAAATTTTCAACACATTTATCTACACGATTTGGGTT‑TGCTTCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTa                                                                                                                                                                                                               <  1:271023/246‑1 (MQ=255)                                                  attgcggtgagattgcc‑gcAACTTCATGAAAAACGGAAATTCCTCTCCTACAGTGAGGAACGAAAAATAAAAAAATTTTTTTTTGGGAATGAAGATTTAATCCCCCCTCATGGGCCAAGGGAAAAAATAAAAAAAGCTTGTC‑CCTT‑GAAACAAAAACAAAATTTCAAACACCCTTATCTACTGAAATTGGGCT‑TCCATCCAAAAAAACA‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCAt                                                                                                                                                                                                          <  2:485379/234‑1 (MQ=255)                                                     tCTGCGATAGTTGC‑GCAATTTCCTAAAAAAAAGGAATATCTCTACTCCAGGGAAGAACGAGAAATGAAGAAACTTTTTTATGTGAATGAAGATTTAATCCCTCCTCCTGGAGCATCGGGAGAAATAATGAACGCGTGCC‑CCTT‑GACACGAATACAAAATTTTAAACACATTTATCTACTCGATTTTGGCT‑TGCACCCGAAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGt                                                                                                                                                                                                       <  1:327723/250‑1 (MQ=255)                                                      cTGCGATAGTTGC‑GCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTC‑CCTT‑GACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATgtggg                                                                                                                                                                                                     >  2:302559/1‑247 (MQ=255)                                                            tAGTTGC‑GCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTC‑CCTT‑GACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAGTAGCGATATTCACCATTAACCATGGTGAGAatat                                                                                                                                                                                               >  2:81897/1‑251 (MQ=255)                                                                       acttcaaaaaaaaCAAGAATTTCTCTCCTAAAGTGATGAACGAATAATAAAGAATCTTTTTTATGTGCATAAAAATTTAACGCCTCCCCCTTGAGCAGCGGGAAAAATAATAAAAGGGTGTC‑CCTT‑GACACAAATAAAAAATTTCCAACACACTTATCTACTCAATTTTGGCT‑TGCACCCAAAAAACCC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCt                                                                                                                                                                                     <  2:112401/243‑1 (MQ=255)                                                                              aaaaaaCAGAATTATTTCTACTAAATTGAGGAACTATAAATGAAGAAATTTTTTTATGTGCATAAAGTTTTAATGCCTCCACCTTGAGCACAGGAAAAAAAAATAAAACCGTGTC‑CCTT‑AACACGAAAAAAAAATTCTCACCCCCCTTATCTCCTAAATTTTGGCT‑TACCTCCAAAAAAACC‑T‑GTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATAc                                                                                                                                                                              <  2:484377/250‑1 (MQ=255)                                                                                  aaCAGGAATATATCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTC‑CCTT‑GACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCa                                                                                                                                                                           >  2:618078/1‑250 (MQ=255)                                                                                   aCAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTC‑CCTT‑GACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAAt                                                                                                                                                                         >  2:318449/1‑251 (MQ=255)                                                                                    cAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTC‑CCTT‑GACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAAtt                                                                                                                                                                        >  2:299147/1‑251 (MQ=255)                                                                                    aaGGAATATCTCTACTACAGAGATGAACGAGTAATGAAAAAATTTTTTTATTTGAAGAAATATTAAATCCCCCCTCTTTGAGCAACGGAAAAAAAAAAGAAAGCGT‑TC‑CCTTTGACACAAAAAAAAAATTTACACCACACTTATCTCCTAAAATTTGGCT‑TCCCCCCAAAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAAtt                                                                                                                                                                        <  2:470659/250‑1 (MQ=255)                                                                                              tctACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTC‑CCTT‑GACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTcc                                                                                                                                                              >  1:582612/1‑251 (MQ=255)                                                                                                    acAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTC‑CCTT‑GACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGt                                                                                                                                                         >  2:760876/1‑250 (MQ=255)                                                                                                          ataaagaagtaaagaaaaagtttttttATGTGAATGAAGATTTAATCCCTCCTCTTTGGGCATGGGGAAAAATAA‑GAAAG‑GTGTCTCCTTTGACATGAATACAAAATTTGAAACCCCCTTATCTCCCCGA‑TTTTGCTGTGCATCCGAAAAAACC‑A‑GGTGACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGAtt                                                                                                                                                  <  1:664027/230‑1 (MQ=255)                                                                                                           agaACGATTAATAAAAAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAAAAATAATGAAAGCGTGTC‑CCTT‑GACACAAATAAAAAATTTTCAACCCACTTATCTCCTCAATTTTGGCT‑TCCATCC‑CAAAAACCCA‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTg                                                                                                                                                 <  1:81897/250‑1 (MQ=255)                                                                                                            aaaCGAGTAATGAAAAATTTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAAAAATAATAAAAGCGTGCC‑CCTT‑GACAAGAATAAAAAATTTTCAACACACTTATCTACTCGATTTTGGCT‑TGCATCCGAAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGc                                                                                                                                                <  1:641516/250‑1 (MQ=255)                                                                                                              aCGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTC‑CCTT‑GACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCAt                                                                                                                                              >  2:99086/1‑251 (MQ=255)                                                                                                               cGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTC‑CCTT‑GACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCAt                                                                                                                                              >  2:299819/1‑250 (MQ=255)                                                                                                                gAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTC‑CCTT‑GACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCAt                                                                                                                                              >  1:111954/1‑249 (MQ=255)                                                                                                                        aagaattttttttATGTGCAGAAAGATTTAATCCCTCCCCCTTGAGCATCGGAAAAAATAAGGAAAGCG‑GTC‑CCTTTGACACAAATAAAAAATTTACAACCCACTTTCCTCCTCAAATTGGGCT‑GCCACCAGAAAAACCC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTa                                                                                                                                    <  2:748212/244‑1 (MQ=255)                                                                                                                                       gtgCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAAGAAAAGCGTGTC‑CCTT‑AACACGAATACAAAATTTTCAACCCATTTATCTCCTCAATTTTGGCT‑TGCACCCGAAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACa                                                                                                                      <  1:709422/250‑1 (MQ=255)                                                                                                                                         gCATAAAGTTTTAATGCCCCCTCCTTGAGCATCGGAAAAAATAATAAAGGCGTGTC‑CCTT‑GACACGAATAAAAAATTTTCAACACCCTTATCTCCTCGATTTTGGCT‑TGCATCCGAAAAACCC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACatat                                                                                                                   <  1:299147/251‑1 (MQ=255)                                                                                                                                          cATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTC‑CCTT‑GACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATAtt                                                                                                                  >  2:711769/1‑251 (MQ=255)                                                                                                                                          cATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTC‑CCTT‑GACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATAtt                                                                                                                  >  1:407911/1‑251 (MQ=255)                                                                                                                                                 aTTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTC‑CCTT‑GACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATCTTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGttt                                                                                                           >  2:744997/1‑251 (MQ=255)                                                                                                                                                                 gtgagcagggggAAAAAAAATAAAAGCGTGCC‑CCTT‑CACAAAAATAAAAATTTTTCAACACATTTATCTACTAAATTTTGTCT‑TGCACCCG‑AAAAACC‑AAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCAcgcc                                                                                           <  2:609300/242‑1 (MQ=255)                                                                                                                                                                             agaAATAATGAAAGCGTGCC‑CCTG‑CACACGAATAAAAAATTTTCACCCCTCTTATCCACTCGATTTGGGCT‑TGCACCCGAAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGt                                                                               <  1:228624/251‑1 (MQ=255)                                                                                                                                                                                   aatGAAAGCTTTCC‑CCTG‑GACACGAATAAAAAATTTTAAACACTCTTATCTACTCAATTTTGGCG‑TGCACCCGAAAAACCC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAat                                                                          <  1:772760/250‑1 (MQ=255)                                                                                                                                                                                   aatGAAAGCGTGTC‑CCTT‑GACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAatt                                                                         >  2:100431/1‑251 (MQ=255)                                                                                                                                                                                    atGAAAGCGTGTC‑CCTT‑GACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAattg                                                                        >  1:361119/1‑251 (MQ=255)                                                                                                                                                                                       aaaGCGTGTC‑CCTT‑GACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAattgatt                                                                     >  1:266922/1‑251 (MQ=255)                                                                                                                                                                                        aaGCGTGTC‑CCTT‑GACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAattgattg                                                                    >  2:24839/1‑251 (MQ=255)                                                                                                                                                                                                  ccTT‑GACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTaa                                                                                                                                   >  1:324561/1‑179 (MQ=255)                                                                                                                                                                                                  ccTT‑GACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGataaataa                                                            >  2:539263/1‑250 (MQ=255)                                                                                                                                                                                                              aTACAAAATTTTCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGCGAATATATTTATGTCTTGCATACGCAATTATACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAActa                                                  >  2:30730/1‑247 (MQ=255)                                                                                                                                                                                                                    aaTTTTCAACACACTTATCTGCTCGACTTTCGCT‑TGCATCCACAAACACA‑A‑GGCCACCGACATTAAAATAGAGATATTCACCATTAACCATGGTGAGAATATATTTATGTTTTGCATAGCCAATTAGACAATTCCCATGTAGGGATTGCATAGTTGACTTAATATTACATAAACCTATTACTGTTTATTAATGCAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAACTTTAAACGGACCGGga                                           >  2:169596/1‑250 (MQ=255)                                                                                                                                                                                                                       tttCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAAc                                       >  1:586186/1‑251 (MQ=255)                                                                                                                                                                                                                         tCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAAc                                       >  1:744240/1‑249 (MQ=255)                                                                                                                                                                                                                            acacacTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGgat                                  >  2:218706/1‑251 (MQ=255)                                                                                                                                                                                                                                acTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTTCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCAGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACCGgatgat                               >  2:595949/1‑250 (MQ=255)                                                                                                                                                                                                                                        actagaattgtgCT‑TGCA‑CCCCAAAAACCCA‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGa                      <  1:299819/240‑1 (MQ=255)                                                                                                                                                                                                                                             atttttgCT‑TGCACCCGAAAAAACA‑A‑GGTCTCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGt                   <  1:302559/243‑1 (MQ=255)                                                                                                                                                                                                                                                        gaaTCCGAAAAACCC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGTTCTACGGGCAgg       <  1:197171/249‑1 (MQ=255)                                                                                                                                                                                                                                                             cacAAAAACC‑A‑GGTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGTTCTACGGGCAGGATGAc  <  1:629639/249‑1 (MQ=255)                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                           GCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGC‑GCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTC‑CCTT‑GACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCT‑TGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGTTCTACGGGCAGGATGAC  >  NC_000913/3665452‑3665950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑