Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (+589/+167)	rtcR → / ← glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	100.0%	371.1 / NA	94	intergenic (+589/+167)	rtcR/glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (48/46);  total (48/46)

ATCTTCTTTTACCATCAACGTGTTAGCAACGTTG‑AGATTGTTGGTG‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTC  >  NC_000913/3560205‑3560698                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    aTCTTCTTTTACCATCAACGTGTTAGCAACGTTG‑AGATTGTTGGTG‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGc                                                                                                                                                                                                                                                      >  2:757805/1‑251 (MQ=255)  tcttctTTTCCCATCAGCGTGT‑AGGAAAGTTGGAGTTTGT‑GGTGGACAATGCGCAATTTGCTGTGTTTGACCAGTGGTTGCGCTCCCGCTTCGGGCGTGGGGCCGATATCGATAAA‑CAGCGTGGACCCATT‑GGGGTTTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCTCACCGCCATGATGGc                                                                                                                                                                                                                                                      <  2:694372/250‑1 (MQ=255)     tcttTTCCCATCAACGTGTTAGCACCGTTG‑AGATGGTTGGTG‑ACAATGCGCAATTTGCTGTGATTGAGCAGTGCGGGCGCTCCCGCTTCCGGCGTGGTGCCAATATCGATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGc                                                                                                                                                                                                                                                      <  1:698434/247‑1 (MQ=255)         ttACCATCAACGTGTTAGCAACGTTG‑AGATTGTTGGTG‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATc                                                                                                                                                                                                                                              >  2:40569/1‑251 (MQ=255)                 aaCGTGTTAGCAACGTTG‑AGATTGTTGGTG‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTg                                                                                                                                                                                                                                      >  2:630379/1‑251 (MQ=255)                 aaCGTGTTAGCAACGTTG‑AGATTGTTGGTG‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTg                                                                                                                                                                                                                                      >  1:327361/1‑251 (MQ=255)                 aaCGTGTTAGCAACGTTG‑AGATTGTTGGTG‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTg                                                                                                                                                                                                                                      >  2:791230/1‑251 (MQ=255)                  aCGTTTTAGCAAGGTTG‑GGTTTGTTGGTG‑ACAATGCTCAAGTGGCGTTGTTTGAGCGGGGCTTGCGTTCCCGCTCCGGGGGTGGTGCCGATA‑CCATAAAACAGCGTCGA‑CCATTTGGGGATTT‑G‑CTCCGCCCCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTACCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGtttt                                                                                                                                                                                                                                       <  2:733966/249‑1 (MQ=255)                  aCGTGTTAGCAACGTTG‑AGATTGTTGGTG‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGACCTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGc                                                                                                                                                                                                                                     >  2:594771/1‑251 (MQ=255)                   cGTGTTAGCAACATTG‑AGATTGTTGGTG‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCt                                                                                                                                                                                                                                    >  1:549436/1‑251 (MQ=255)                    gtgtTAGCAACGTTG‑AGATTGTTGGTG‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTc                                                                                                                                                                                                                                   >  2:231584/1‑251 (MQ=255)                      tttAGAAACGTTG‑AGATTGTGGGTG‑ACAATGCGAAAGTTCCTGTGATTGAGCAGTGCGTCCGTTACCGCTCCCGGCGTGGTGCCGATTCCGATAAC‑CAGCGTCGAGCCATG‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTcc                                                                                                                                                                                                                                  <  2:786769/249‑1 (MQ=255)                            aaCGTTG‑AGATTGTTGGTG‑ACAATGCGCAAATTGCTGTGTTTGACCAGTGCGTGCTCTACCGTTCCCGGCGTGGTGCCGATATCGATAAC‑CAGCGTCGGGCCATT‑GGGGATTT‑C‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCTGTCTGGGTGG‑CTTTCCGATCGTTCCACGGCGTGTTAACCGAACTGGATGGCAGCCCCGCACCGCCATGATGGCGCAGGATCAGGttt                                                                                                                                                                                                                                        <  1:44943/238‑1 (MQ=255)                            aaCGTTG‑AGATTGTTGGTG‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGttt                                                                                                                                                                                                                                        >  2:44943/1‑238 (MQ=255)                              cGTTG‑AGATTGTTGGTG‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGAGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTGGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCAATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTc                                                                                                                                                                                                                          >  1:365866/1‑250 (MQ=255)                                 tG‑AGATTGTTGGTG‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTg                                                                                                                                                                                                                      >  1:624266/1‑251 (MQ=255)                                       ttgttgGTG‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTc                                                                                                                                                                                                                 >  1:130101/1‑251 (MQ=255)                                             gTG‑ACAATGCGAAATTTGCGGTGATTGAGCAGTGCGTGCGCTCCCGCTTCCGGCGTGGGGCCGATATCGATAAA‑CAGCGTCGGGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggcg                                                                                                                                                                                                           <  1:655358/251‑1 (MQ=255)                                              tG‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggcg                                                                                                                                                                                                           >  2:118591/1‑250 (MQ=255)                                               g‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGa                                                                                                                                                                                                          >  1:624071/1‑250 (MQ=255)                                                     tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTc                                                                                                                                                                                                    >  1:253686/1‑251 (MQ=255)                                                      gcgaaaaTTGCTGTGATGGAGCAGTGCGGGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCt                                                                                                                                                                                                   <  1:787902/247‑1 (MQ=255)                                                           aaTTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCggg                                                                                                                                                                                              >  1:386742/1‑251 (MQ=255)                                                           aaTTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCggg                                                                                                                                                                                              >  1:645747/1‑251 (MQ=255)                                                              tgctgtatttGAGCAGGGCGTGCGCTACCGTTTCCGGCGTGGTGCCGATATCGATAAC‑CGGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCt                                                                                                                                                                                            <  1:78238/242‑1 (MQ=255)                                                                      ttGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATCGTCTGCGGGCTGACGgaga                                                                                                                                                                                    >  2:75976/1‑250 (MQ=255)                                                                      ttGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgaga                                                                                                                                                                                    >  1:515835/1‑250 (MQ=255)                                                                       tGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgagac                                                                                                                                                                                   >  1:270193/1‑249 (MQ=255)                                                                         aGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATg                                                                                                                                                                                >  2:111351/1‑251 (MQ=255)                                                                           cAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGct                                                                                                                                                                              >  2:302626/1‑251 (MQ=255)                                                                                  ggcgcTCCCGCTTCGGGCGTGGGGCCGTTTTCGAAAAA‑CAGGGGCGAGC‑GTT‑GGGGGTTT‑GGCCCCGCCACTTTGCGGGCGGTGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAg                                                                                                                                                                       <  2:502534/250‑1 (MQ=255)                                                                                     gctcccGCTTCCGGCGTGGTGCGGATATCGATAAA‑CAGCGTCGGGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGctc                                                                                                                                                                    <  1:600947/247‑1 (MQ=255)                                                                                       tACCGCTTCCGGCGTGGTGCCGATATCGATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt                                                                                                                                                                  >  2:40714/1‑251 (MQ=255)                                                                                        cccGCTTCCGGGGTGGTGCGAATATCGAAAAC‑CGGGGTCGAGCCTTT‑GGGGTTTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTc                                                                                                                                                                 <  1:79824/250‑1 (MQ=255)                                                                                        cccGCTTCCGGCGTGGGGCCAATATCGATAAA‑CAGCGTCGAGCCTGG‑GGGGTTTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGctct                                                                                                                                                                   <  2:515835/248‑1 (MQ=255)                                                                                           gCTTCCGGCGTGGTGCCGATATCGATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGt                                                                                                                                                              >  2:642170/1‑251 (MQ=255)                                                                                              tCCGGCGTGGTGCCGATATCAATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                                                                                                                                            <  1:642170/250‑1 (MQ=255)                                                                                                      ggtgCCGATATCGATAAA‑CGGGGTCGGGCCTTT‑GGGGTTTT‑C‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCGTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAc                                                                                                                                                   <  1:27670/251‑1 (MQ=255)                                                                                                      ggtgCCGATATCGATAAA‑CAGCGTCGGGCCTTT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATaa                                                                                                                                                    <  1:630379/250‑1 (MQ=255)                                                                                                         gcggATATCGATAAA‑CGGCGTCGGGCCTTT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                                                                                                                                <  1:375913/248‑1 (MQ=255)                                                                                                               atCGATAAA‑CAGCGTCGAGCCATG‑GGGGTTTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGt                                                                                                                                           <  1:236037/250‑1 (MQ=255)                                                                                                                   aTAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc                                                                                                                                      >  2:541677/1‑251 (MQ=255)                                                                                                                    ataaccggCGTCGAGCCATT‑GGGGTTTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc                                                                                                                                      <  1:75976/244‑1 (MQ=255)                                                                                                                     aaa‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAcc                                                                                                                                     >  2:503594/1‑250 (MQ=255)                                                                                                                      aa‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTc                                                                                                                                                                                                                          >  2:732285/1‑164 (MQ=255)                                                                                                                      aa‑CAGCGTCGAGCCATG‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTc                                                                                                                                                                                                                          <  1:732285/164‑1 (MQ=255)                                                                                                                          ggggggagcccttt‑GGGGTTTG‑G‑CCCCGCCCCTTGGCGGGCGATGCGCTCTTTTTCTTCGGTCGGGGGGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTc                                                                                                                                <  2:257731/239‑1 (MQ=255)                                                                                                                                gAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataat                                                                                                                          >  1:578681/1‑251 (MQ=255)                                                                                                                                  gCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAc                                                                                                                        <  1:791230/251‑1 (MQ=255)                                                                                                                                  cccATT‑GGGGTTTT‑G‑CTCCCCCCCTTTGCGGGCGTTGCGCTTTTTTTCTTCGTTCTTGGTGG‑CCTTGCGATCTTGCCCCGGCGTGTTAACCGAACTGGAAGGCGGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAc                                                                                                                        <  1:533242/250‑1 (MQ=255)                                                                                                                                   gcATT‑GGGGTTTTTG‑CGCCGCCACTTTGGGGGCGAGGCGCTCTTTTTCTTCGGGC‑GGGTGGGCCTTGCGGCCGTGCCACGGCGTGTTACCCAAACGGAAAGGCCGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAc                                                                                                                        <  2:719794/250‑1 (MQ=255)                                                                                                                                   ccATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCACTCTTTTTCTTCGGTCTGGGTGG‑GCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAc                                                                                                                        >  2:672892/1‑250 (MQ=255)                                                                                                                                         ggggTTTT‑G‑CTCCGCCACTTGGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCCCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgt                                                                                                                  <  1:790165/251‑1 (MQ=255)                                                                                                                                                  g‑CTCCGCCACTTGGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtg                                                                                                           <  1:231584/250‑1 (MQ=255)                                                                                                                                                      ccGCCCCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtt                                                                                                       <  1:118591/251‑1 (MQ=255)                                                                                                                                                           ccTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTgtttgttt                                                                                                  <  1:757805/250‑1 (MQ=255)                                                                                                                                                              ttGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGCTGTGTTTGTTTc                                                                                                 <  1:541677/249‑1 (MQ=255)                                                                                                                                                              tggCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCGTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGGGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCa                                                                                                <  2:624071/248‑1 (MQ=255)                                                                                                                                                                gCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGGGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCAttt                                                                                             <  1:557348/251‑1 (MQ=255)                                                                                                                                                                 gtggggctGCGCTCTTTTTCTTGGGTCAGGGTGG‑CCGTGTGATCGTCCCACGGGGTGTTGACCAAATTGGAAGTCTCGGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTta                                                                                            <  2:93748/244‑1 (MQ=255)                                                                                                                                                                 ggggCGATGCGCTCTTTTTCTCCTGTCTGGGTGC‑CCGTGGTTTCGTGCCGCGGTGTGTTAACCGATCTGGAAGGCAGGGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTTTTCGGTACTGACATAGCCCTGCTGTTTAACCAGTGCGATAATACCGTTGTGACGTTTTGTTTGTTTCATTta                                                                                            <  2:270193/250‑1 (MQ=255)                                                                                                                                                                 cGGGCGATGCGCTCTTTTTCTTCGGTTTGGGTGG‑CCTTGCGTTCGTGCCACGGCGTGTTAACGGACCTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTCCTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTta                                                                                            <  1:429695/251‑1 (MQ=255)                                                                                                                                                                   ggCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTtat                                                                                           >  1:75057/1‑250 (MQ=255)                                                                                                                                                                        tGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATcc                                                                                     >  1:380428/1‑251 (MQ=255)                                                                                                                                                                         gcgcTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATcc                                                                                     <  1:690439/250‑1 (MQ=255)                                                                                                                                                                                 tttCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAatt                                                                             >  1:519304/1‑250 (MQ=255)                                                                                                                                                                                  ttcttcGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAattat                                                                           >  2:295185/1‑251 (MQ=255)                                                                                                                                                                                      tcGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttc                                                                       >  1:137148/1‑251 (MQ=255)                                                                                                                                                                                           ccctacccgcctctcttccgatctaCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgttt                                                                   <  2:248115/227‑1 (MQ=255)                                                                                                                                                                                             gggTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgttttcg                                                                >  2:588946/1‑251 (MQ=255)                                                                                                                                                                                              ggTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTcgc                                                               <  2:386742/251‑1 (MQ=255)                                                                                                                                                                                               gTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTcgcg                                                              >  2:667780/1‑251 (MQ=255)                                                                                                                                                                                                 gg‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCgcgc                                                             >  1:265207/1‑250 (MQ=255)                                                                                                                                                                                                  g‑CCTGGCGATCGCGCCACGGCGTGTTAACCGAACTGGAAGGCCGCGCCGCGCCGCCAGGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCAt                                                           <  2:519304/251‑1 (MQ=255)                                                                                                                                                                                                    ccTTGCGATCGTGCCCCGGCGTGTTAACCGAACGGGAAGGCAGCGCCCCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCAtt                                                          <  2:210415/251‑1 (MQ=255)                                                                                                                                                                                                     cTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTg                                                         >  1:214473/1‑251 (MQ=255)                                                                                                                                                                                                         cGATCGTGCCACGGCGGGTTAACCGAACGGGAAGGCGGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCg                                                     <  2:640060/251‑1 (MQ=255)                                                                                                                                                                                                          gATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCg                                                     >  1:728632/1‑250 (MQ=255)                                                                                                                                                                                                          gATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCg                                                     >  1:236237/1‑250 (MQ=255)                                                                                                                                                                                                           aTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGa                                                    >  2:302734/1‑250 (MQ=255)                                                                                                                                                                                                                  cACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaacaa                                            <  1:667780/251‑1 (MQ=255)                                                                                                                                                                                                                   aCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgttt                                                                   >  1:248115/1‑227 (MQ=255)                                                                                                                                                                                                                     ggCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAg                                         <  1:111351/251‑1 (MQ=255)                                                                                                                                                                                                                          gtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATc                                    >  1:187981/1‑251 (MQ=255)                                                                                                                                                                                                                          gtTAACCGAACTGGAAGGACGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATc                                    <  2:130101/251‑1 (MQ=255)                                                                                                                                                                                                                              aCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt                                 >  2:654805/1‑250 (MQ=255)                                                                                                                                                                                                                                   acggtaaggcggggccgcACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAccc                           <  2:582430/238‑1 (MQ=255)                                                                                                                                                                                                                                   aCTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAccc                           <  1:302626/251‑1 (MQ=255)                                                                                                                                                                                                                                   aCTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGGTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAcac                           >  1:262084/1‑249 (MQ=255)                                                                                                                                                                                                                                    gtGGAAGGCGGCGCCGCCCCGCCATGAGGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATGTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCa                          <  2:262084/250‑1 (MQ=255)                                                                                                                                                                                                                                          ggCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTa                    <  1:40714/251‑1 (MQ=255)                                                                                                                                                                                                                                             aGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAcc                 >  1:417297/1‑251 (MQ=255)                                                                                                                                                                                                                                                 ccgcACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGCATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAACCGGc             >  2:331794/1‑251 (MQ=255)                                                                                                                                                                                                                                                   gcACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCg            >  1:769435/1‑250 (MQ=255)                                                                                                                                                                                                                                                          cATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGc    <  2:624266/251‑1 (MQ=255)                                                                                                                                                                                                                                                           atgatgGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCt   >  1:364802/1‑251 (MQ=255)                                                                                                                                                                                                                                                            ggatgGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTc  <  2:253686/250‑1 (MQ=255)                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    ATCTTCTTTTACCATCAACGTGTTAGCAACGTTG‑AGATTGTTGGTG‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAA‑CAGCGTCGAGCCATT‑GGGGATTT‑G‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGG‑CCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTC  >  NC_000913/3560205‑3560698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑