Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,449,779	G→A	S35S (AGC→AGT)	rpsC ←	30S ribosomal subunit protein S3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,449,779	0	G	A	100.0%	292.2 / NA	75	S35S (AGC→AGT)	rpsC	30S ribosomal subunit protein S3
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/40);  total (35/40)

AGCAACCAGTTTTGCGTCCAGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCGCTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACT  >  NC_000913/3449533‑3450024                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      aGCAACCAGTTTTGCGTCCAGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGt                                                                                                                                                                                                                                                   <  2:10572/251‑1 (MQ=255)   cAACCAGTTTTGCGTCCAGGTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGAGACGGCCGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGGACTTTAAAATCACTGTcc                                                                                                                                                                                                                                                 >  2:7214/1‑251 (MQ=255)    aaCCAGTTTTGCGTCCAGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTcc                                                                                                                                                                                                                                                 >  1:98212/1‑250 (MQ=255)      ccAGTTTTGCGTCCAGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAgg                                                                                                                                                                                                                                              <  1:485617/251‑1 (MQ=255)        aGTTTTGCGTCCAGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGtt                                                                                                                                                                                                                                            <  2:159231/251‑1 (MQ=255)        aGTTTTGCGTCCAGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGtt                                                                                                                                                                                                                                            <  1:220621/251‑1 (MQ=255)               ngTCCAGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCg                                                                                                                                                                                                                                     >  2:334758/2‑251 (MQ=255)               cGTCCAGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCg                                                                                                                                                                                                                                     >  1:397622/1‑251 (MQ=255)                   cAGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAAtt                                                                                                                                                                                                                                 >  2:45967/1‑251 (MQ=255)                           gCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGgtg                                                                                                                                                                                                                         <  1:658790/251‑1 (MQ=255)                           gCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGgtg                                                                                                                                                                                                                         <  2:397622/251‑1 (MQ=255)                             ttACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGtt                                                                                                                                                                                                                       >  2:223338/1‑251 (MQ=255)                                        ggCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAgg                                                                                                                                                                                                            >  2:389892/1‑251 (MQ=255)                                           gATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGt                                                                                                                                                                                                           >  2:519783/1‑249 (MQ=255)                                            aTGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGt                                                                                                                                                                                                           >  1:134937/1‑248 (MQ=255)                                              gTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGt                                                                                                                                                                                                      <  2:444432/251‑1 (MQ=255)                                                      gtgCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTcc                                                                                                                                                                                                   <  2:134091/246‑1 (MQ=255)                                                      gtgCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCAt                                                                                                                                                                                                 <  1:204787/248‑1 (MQ=255)                                                      gtgCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGt                                                                                                                                                                                              <  1:223338/251‑1 (MQ=255)                                                      gtgCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGGGTTCGCAAACCAGGTAGAGTTCCATGGt                                                                                                                                                                                              >  1:211705/1‑251 (MQ=255)                                                           ggAACTCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTAc                                                                                                                                                                                         <  2:509131/251‑1 (MQ=255)                                                               cGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAAt                                                                                                                                                                                      >  1:162385/1‑250 (MQ=255)                                                                      gATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGc                                                                                                                                                                              >  2:282275/1‑251 (MQ=255)                                                                       aTGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCg                                                                                                                                                                             <  1:191096/251‑1 (MQ=255)                                                                                aCGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCAtt                                                                                                                                                                    <  2:79416/251‑1 (MQ=255)                                                                                       tACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGAt                                                                                                                                                               <  1:502157/249‑1 (MQ=255)                                                                                           cAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACtt                                                                                                                                                         >  2:656939/1‑251 (MQ=255)                                                                                                  tCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAAtt                                                                                                                                                                                                                                 <  2:434393/172‑1 (MQ=255)                                                                                                  tCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAAtt                                                                                                                                                                                                                                 >  1:434393/1‑172 (MQ=255)                                                                                                      cGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCAtt                                                                                                                                              <  2:82210/251‑1 (MQ=255)                                                                                                       gTCTTCACCTTTTTTACCGATAACGATACCCGTGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTg                                                                                                                                             <  1:356875/251‑1 (MQ=255)                                                                                                          ttCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCt                                                                                                                                           >  1:201118/1‑250 (MQ=255)                                                                                                              ccTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGtct                                                                                                                                      >  1:156290/1‑251 (MQ=255)                                                                                                               cTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACGATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGtctc                                                                                                                                     >  1:324302/1‑251 (MQ=255)                                                                                                                            aaCGATACCCGGGCGGGCAGTGTGAATGGTTACACGGATGCTTTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGc                                                                                                                         <  1:206687/250‑1 (MQ=255)                                                                                                                                     cGGGCGAGCAGTATGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGacac                                                                                                               >  1:512074/1‑251 (MQ=255)                                                                                                                                       ggCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGAcaca                                                                                                              >  1:90237/1‑250 (MQ=255)                                                                                                                                         cGAGCAGTGTTAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATGCCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGTACACAAcc                                                                                                           <  2:211705/251‑1 (MQ=255)                                                                                                                                         cGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAAcc                                                                                                           >  2:421798/1‑251 (MQ=255)                                                                                                                                         cGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAAcc                                                                                                           >  2:137684/1‑251 (MQ=255)                                                                                                                                                 gtgAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGAt                                                                                                   <  1:341546/251‑1 (MQ=255)                                                                                                                                                    aaTGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGAtgtg                                                                                                <  1:334758/251‑1 (MQ=255)                                                                                                                                                        nttACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTggttg                                                                                            >  2:323458/2‑248 (MQ=255)                                                                                                                                                             acGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTgcg                                                                                       >  1:134403/1‑251 (MQ=255)                                                                                                                                                               ggATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCt                                                                                     >  1:695925/1‑251 (MQ=255)                                                                                                                                                                gATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCtt                                                                                    <  1:519783/251‑1 (MQ=255)                                                                                                                                                                        tAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGAt                                                                              <  1:552781/249‑1 (MQ=255)                                                                                                                                                                         aGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGc                                                                            <  2:162385/250‑1 (MQ=255)                                                                                                                                                                             ggACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATGCCCAGGCGAATACCATTAGGATGTTCTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCt                                                                       <  1:346050/251‑1 (MQ=255)                                                                                                                                                                             ggACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCt                                                                       <  2:64807/251‑1 (MQ=255)                                                                                                                                                                                  cTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACg                                                                  >  1:415951/1‑251 (MQ=255)                                                                                                                                                                                         aCGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATc                                                                                                                     >  1:270504/1‑193 (MQ=255)                                                                                                                                                                                         aCGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATc                                                                                                                     <  2:270504/193‑1 (MQ=255)                                                                                                                                                                                         aCGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCtttt                                                           <  2:415951/251‑1 (MQ=255)                                                                                                                                                                                           gATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGc                                                         >  1:290105/1‑251 (MQ=255)                                                                                                                                                                                           gATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGc                                                         >  1:51725/1‑251 (MQ=255)                                                                                                                                                                                               cGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCAcgc                                                     >  1:718501/1‑251 (MQ=255)                                                                                                                                                                                                gagaTACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCAcgcg                                                    >  1:377414/1‑251 (MQ=255)                                                                                                                                                                                                   aTACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGc                                                  >  1:293484/1‑250 (MQ=255)                                                                                                                                                                                                        gACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGGGCGCTTCAGGATGCGAGCTGCACGACCTTTTTCACGCGGCATAATg                                            >  2:312352/1‑251 (MQ=255)                                                                                                                                                                                                               tAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTc                                      <  1:137684/250‑1 (MQ=255)                                                                                                                                                                                                                aGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCAt                                    >  1:117628/1‑251 (MQ=255)                                                                                                                                                                                                                            aGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCtt                         <  2:134937/250‑1 (MQ=255)                                                                                                                                                                                                                            aGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTc                        <  1:312352/251‑1 (MQ=255)                                                                                                                                                                                                                               cAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGt                      <  1:656939/250‑1 (MQ=255)                                                                                                                                                                                                                                     cTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGa                >  1:431422/1‑250 (MQ=255)                                                                                                                                                                                                                                       gACGTACTTTAAAATCACTGTCCAGGTTTTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGaaa              <  1:45967/250‑1 (MQ=255)                                                                                                                                                                                                                                       gACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATTCCCAGGCGAATACCATTAGGATGTTCTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGTACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGaaaa             <  1:323458/251‑1 (MQ=255)                                                                                                                                                                                                                                       gACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCCGAGTCTCAGCGATCGGACACAACCACAGTGATGTTGCTGTTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGaaa              <  2:596059/250‑1 (MQ=255)                                                                                                                                                                                                                                       gACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCTTTCGTCTACGaa               <  1:520976/249‑1 (MQ=255)                                                                                                                                                                                                                                                 aaaaTCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTaa    <  2:542981/250‑1 (MQ=255)                                                                                                                                                                                                                                                 aaaaTCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAAc   <  1:482692/251‑1 (MQ=255)                                                                                                                                                                                                                                                  aaaTCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACt  <  1:530162/251‑1 (MQ=255)                                                                                                                                                                                                                                                   aaTCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCAc                                                       <  2:168053/197‑1 (MQ=255)                                                                                                                                                                                                                                                   aaTCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCAc                                                       >  1:168053/1‑197 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      AGCAACCAGTTTTGCGTCCAGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCGCTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACT  >  NC_000913/3449533‑3450024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑