Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,123,930	A→T	V176E (GTA→GAA)	cytR ←	DNA‑binding transcriptional repressor CytR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,123,930	0	A	T	100.0%	251.1 / NA	64	V176E (GTA→GAA)	cytR	DNA‑binding transcriptional repressor CytR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (29/35);  total (29/35)

AGCGCCATCACATCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTACTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑TGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCG  >  NC_000913/4123684‑4124169                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  aGCGCCATCACATCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGGAGGGACAAAGCGGCATCTCTTCGGGACCGGCTATACAGGCAAACCGTTTATGCCCCTGCTCATATAAATAAATTTCTGc                                                                                                                                                                                                                                               >  2:547441/1‑251 (MQ=255)    gcCATCACATCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATc                                                                                                                                                                                                                                            >  1:175833/1‑251 (MQ=255)     ccATCACATCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATc                                                                                                                                                                                                                                            >  1:8365/1‑250 (MQ=255)      cATCACATCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGCAGTGACACAGCGGCATCTCTTCGGGACCGGCTATAAAGCCCAACCGGTTATGCCCCTGCCCCTATAAATAATTTTCTGCATCa                                                                                                                                                                                                                                           >  2:170006/1‑250 (MQ=255)            aTCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGGCCGGCTATACAGCCAATCCGTTTTTGCCCTTTCTCATATAAATAATTTTCTGCATCAAATgcgg                                                                                                                                                                                                                                    >  2:37453/1‑251 (MQ=255)              cGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCCTATAAATAATTTTCTGCATCAAATgcggcg                                                                                                                                                                                                                                  >  2:244604/1‑251 (MQ=255)                        gaagaCAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCCCTTCGGGACCGGCTTTACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCCTCAACTGCGGGGGTCAgcatgt                                                                                                                                                                                                                        >  2:485067/1‑246 (MQ=255)                              agcagTAGGCGGTTATGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTAGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCGCCTCGGGACCGGGCATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCCACAAATGCGGCGGTCAGATTGTCCatat                                                                                                                                                                                                                  >  2:715853/1‑251 (MQ=255)                                 agTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACCGCGGCATCTCTTCGGGGCCGGCCATACAGACCATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTccgaatgtgagtagta                                                                                                                                                                                                                >  1:115498/1‑235 (MQ=255)                                            gtgGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGcagc                                                                                                                                                                                                    <  1:357237/251‑1 (MQ=255)                                            gtgGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGcagc                                                                                                                                                                                                    <  1:210240/251‑1 (MQ=255)                                              ggAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGcagct                                                                                                                                                                                                   <  1:173881/250‑1 (MQ=255)                                               gAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGcagctcc                                                                                                                                                                                                 <  2:175833/251‑1 (MQ=255)                                                aaGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTTTACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTTTCGATATTAACTGTCGGcagctccc                                                                                                                                                                                                >  1:394873/1‑250 (MQ=255)                                                aaGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGGTATTAACTGTAGGcagctccc                                                                                                                                                                                                >  1:85557/1‑250 (MQ=255)                                                             tgctgcATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTTAGCAGCTCCCGCTCCGGTGcca                                                                                                                                                                                    >  2:728664/1‑248 (MQ=255)                                                                 gcATCGCTTTGCTTCCTGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAAGCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCTTTGCAAAtt                                                                                                                                                                                 <  2:115498/249‑1 (MQ=255)                                                                 gcATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAAttc                                                                                                                                                                                <  2:410633/250‑1 (MQ=255)                                                                           gCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTTTCGATATGAAATGTAGGGAGCTCCAGCCCCGGGGCAAAttcgttccccctcc                                                                                                                                                                     >  2:35438/1‑244 (MQ=255)                                                                                     tCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATcggc                                                                                                                                                             <  2:95005/249‑1 (MQ=255)                                                                                       gAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCCGCTCCGGTGCAAATTTGTTTGCCATCCACATcggccgcc                                                                                                                                                         >  1:697855/1‑247 (MQ=255)                                                                                         aGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGa                                                                                                                                                      <  2:500905/251‑1 (MQ=255)                                                                                          ggTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCCATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCCGCTCCGGGGCAAATTCGGTCGCCATCACCATCGGCGGC‑AGa                                                                                                                                                      >  1:363317/1‑250 (MQ=255)                                                                                           gTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGAAACAGCGGCATCTCTTCGGGACCGGCTATACAGCCCATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTTTCGCTATTAACTGTTGGCAGCTCCCGCTCCGGGGGAAATTTGTTTGCCATCACCATCGGCGGA‑AGAtt                                                                                                                                                    >  1:554211/1‑251 (MQ=255)                                                                                             gAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTa                                                                                                                                                   <  1:241298/250‑1 (MQ=255)                                                                                             gAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTa                                                                                                                                                   <  1:139499/250‑1 (MQ=255)                                                                                                  cGCCACGGGCGATGTAGTGCGGATCAACCATAATGCCGCAGCGACGCAGCGGCTGAACAGAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTGCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTGCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑TGt                                                                                                                                            <  2:676264/251‑1 (MQ=255)                                                                                                       cGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑TGTTCCTc                                                                                                                                       <  2:258332/251‑1 (MQ=255)                                                                                                        gggCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑TGTTCCTCa                                                                                                                                      <  1:460485/251‑1 (MQ=255)                                                                                                         ggCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAACTCCGGTGCAAATTCGTTCGCCCTCACCATCGGCGGC‑CGATTTCGC‑TGTTCCTCa                                                                                                                                      >  1:8794/1‑250 (MQ=255)                                                                                                           cGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑TGTTCCTCAATg                                                                                                                                   <  1:728664/251‑1 (MQ=255)                                                                                                             aTGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑TGTTCCTCAATGCt                                                                                                                                 <  2:519080/251‑1 (MQ=255)                                                                                                                 aTTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑TGTTCCTCAAt                                                                                                                                    <  1:103009/244‑1 (MQ=255)                                                                                                                 aTTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGGCAGATTGTCGGTATGAACTGTAGGCAGCTCCCGCTCCGGGGCAAATTTGTTCGCCATCACCATCGGCGGC‑CGATTACGG‑TGTTtctccatagaacgg                                                                                                                             >  2:103009/1‑241 (MQ=255)                                                                                                                    gCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑TGTTCCTCAATGCTGGCATCa                                                                                                                          <  1:695071/251‑1 (MQ=255)                                                                                                                          cAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑TGTTCCTCAATGCTGGCATCAAACgg                                                                                                                     <  2:8365/250‑1 (MQ=255)                                                                                                                          cAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑TGTTCCTCAATGCTGGCATCAAACGGc                                                                                                                    <  1:527744/251‑1 (MQ=255)                                                                                                                                        cAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑TGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAg                                                                                                      <  1:35438/251‑1 (MQ=255)                                                                                                                                         aGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTTAGATTTTCGGTATTAAATGGAGGCAGCTCCAGCTCCCGTGCCAATTTGTTCGCCATCCCCATCCGCGGG‑AGATTAACC‑TGTTCCTCAAATCTTGCCTCAAACGGCAGCCCTTAACCCAGc                                                                                                     >  1:723467/1‑251 (MQ=255)                                                                                                                                            gACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCCACTTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCCAATTCGTTCCCCATCACCATCGGCGGC‑AGATTACGC‑TGTTTCTCAATGCCGGCCTCAAACGGCAGCCTTGAAACCAGcaa                                                                                                   >  1:464451/1‑250 (MQ=255)                                                                                                                                            gACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTTTCGGTATGAAATGTAGGCAACTCCAGCCCCGGGGGCAATTTGTTTGCCATCACCCTCGGCGGCCGGATT‑CGCCTTTTCC‑CAAAGCTGGCATCAAACGGCAGCCCTGAAACCAGcaac                                                                                                  >  2:47683/1‑251 (MQ=255)                                                                                                                                               gCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑TGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCaacaac                                                                                               <  2:481699/251‑1 (MQ=255)                                                                                                                                                 aGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑TGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACAt                                                                                             <  1:39920/251‑1 (MQ=255)                                                                                                                                                      cTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGGAGCTCCAGCTCCGGTGCAAAATCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑CGTTTCTCAATGCTGGCATCCAACCGCAGCCCTGAACCCAGCAACAACATTCCAt                                                                                        >  1:531112/1‑251 (MQ=255)                                                                                                                                                                      aGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑TGTTCCTCCATGCTGGCATCAAACGGCAGGCCTGAACCCAGCAACAAAATGCCATCAATTTGCtt                                                                              >  1:488727/1‑245 (MQ=255)                                                                                                                                                                      aGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTGTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑TGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCtt                                                                              <  2:488727/245‑1 (MQ=255)                                                                                                                                                                              gTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑TGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAAt                                                                <  1:547441/251‑1 (MQ=255)                                                                                                                                                                                gACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑TGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCg                                                              <  2:246692/251‑1 (MQ=255)                                                                                                                                                                                  cacaGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑TGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGAt                                                            <  1:485067/251‑1 (MQ=255)                                                                                                                                                                                  cacaGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑TGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGAt                                                            >  1:30589/1‑251 (MQ=255)                                                                                                                                                                                      gCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATGGGCGGC‑AGATTACGC‑TGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAg                                                        <  1:445885/251‑1 (MQ=255)                                                                                                                                                                                               ctTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑TGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGtttttt                                                 <  2:731949/249‑1 (MQ=255)                                                                                                                                                                                                  cGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑CGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCctgc                                            <  2:531112/251‑1 (MQ=255)                                                                                                                                                                                                      aCCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑TGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGAt                                        <  1:37453/251‑1 (MQ=255)                                                                                                                                                                                                             ataCAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑Tg                                                                                                                                             <  1:19324/143‑1 (MQ=255)                                                                                                                                                                                                             ataCAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑Tg                                                                                                                                             >  2:19324/1‑143 (MQ=255)                                                                                                                                                                                                                 aGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑TGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTTCTTGGGGATGATCAAATCGGTAAAGGGTTTTTCCTGCTGGTTTTGATgcgcc                             >  2:43674/1‑250 (MQ=255)                                                                                                                                                                                                                         cGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCTGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑TGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGcc                     <  2:353219/251‑1 (MQ=255)                                                                                                                                                                                                                                 cccTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑TGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGc              >  1:622241/1‑250 (MQ=255)                                                                                                                                                                                                                                      gCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGGAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGcc                                                                                                                                                                         <  2:632329/92‑1 (MQ=255)                                                                                                                                                                                                                                      gCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGGCGATATGAACTTTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGcc                                                                                                                                                                         >  1:632329/1‑92 (MQ=255)                                                                                                                                                                                                                                            ataaataaTTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑TGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATcc   <  1:701555/250‑1 (MQ=255)                                                                                                                                                                                                                                            ataaataaTTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAAATGTAGGAAGCCCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTAAGC‑TGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGGGATGATTAAATCGATCAAGGGTTTTTCCTGCTGAATTTGAAGCGCACAGTCCCCAATCAGCACCCGGTATCCg  >  1:214759/1‑251 (MQ=255)                                                                                                                                                                                                                                            ataaataaTTTTCTGCAACAAATGCGGCGGTCAGATGGGCGATATGAACTGGAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑TGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAAACGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATcc   <  2:394873/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  AGCGCCATCACATCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTACTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGC‑AGATTACGC‑TGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCG  >  NC_000913/4123684‑4124169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑