| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,388,570 | 0 | A | C | 27.3% | 188.7 / 10.1 | 77 | V186G (GTG→GGG) | aaeA | aromatic carboxylic acid efflux pump membrane fusion protein |
| Reads supporting (aligned to +/- strand): ref base A (45/11); new base C (0/21); total (45/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.23e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCA‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCT > NC_000913/3388322‑3388819 | tGTCGAGACGAATTCGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCA‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCan < 2:320852/250‑2 (MQ=255) cGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCA‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAgc > 1:319429/1‑251 (MQ=255) gaCGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCA‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAgcgc > 1:60654/1‑250 (MQ=255) gAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCA‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAAcc > 2:532958/1‑251 (MQ=255) aCGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCA‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAAccgcc < 2:359257/250‑1 (MQ=255) ccGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCA‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGAt > 2:349435/1‑251 (MQ=255) cgcAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCA‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGtaata > 1:222630/1‑250 (MQ=255) aGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCA‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTc > 2:289504/1‑251 (MQ=255) cGCACCCATTAAAGGTTAGATTCTATAGTCGCCACCCCTTGGTCGTCACGCGTGCTGCTGGCGTGGGTGACCCCGGCGGCAACACTATCAACAGTCCCTTTCA‑GCATTTTTTTACTGCCAAGCGGCGTTTTCTCTGCACGATCCCCCGGACGCACCCCTCCCAGCTTATTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTCCCCCAGCGCAACCGCCGTTGATCCTCGATTAATAAACTCAcc < 1:228931/251‑1 (MQ=255) gCACCAATTCAAGTTTAGATTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCTTTCCA‑GCACTTTTTTACGGCCAAGCGGCGTATTCTCGCCACAATCCCCCGGACGCACCCCTCCCAGCTTATTTT‑TTTCCATATAGCCCAGTACATAGAAGGAGTT‑CGTTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCg < 1:47978/251‑1 (MQ=255) aaGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCA‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGt > 1:243181/1‑251 (MQ=255) agTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCA‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTg > 1:241306/1‑251 (MQ=255) agTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCA‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTg > 1:317041/1‑251 (MQ=255) tCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCA‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGt > 1:403211/1‑251 (MQ=255) tatggcccccctcccttttccaccccgcgTGCGGCTGGCGTTGGTGCCCCCTGCGGCAACACTATCAACGGTCCCTTTCA‑GCTCTTTTTTCCTGCAAGGCGGCGTGATCTCTGCACGATCCCCCGGACGCCCCCCTCCCAGTTTATTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCCCCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTc < 2:273222/225‑1 (MQ=255) ataGTCGCCATCCCTTGGTCGTCACGGGGGTTGCTGGCGTTGTTGCCCCCTGCGGCAACCCTATCAACAGTCCCTTTCA‑CCATTTTGTTACTGCCAAGCGGGGTGATCTCTGCACGATCCCCCGGACCCACCCCTTCGAG‑TTAGTTTTCTTCCAGATAGGCCATTATATAGAAGGCGTT‑CTTTTTCCCCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCa < 1:605843/250‑1 (MQ=255) ataGGCGCCATCCCTTTGTCTTCCCGCGGGCGGCTGGCGTTGGTGCCCCCGGCGGCAACACTATCAACGGTCCCTTTCA‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCGCGATCCCCCGGCCGCCCCCCTTCCAGCTTAGTTT‑CTCCCATATAGTCCAGTACATAGAGGGAGTT‑CTGTTTCCCCATCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCa < 1:410428/250‑1 (MQ=255) aGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCA‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCAccc > 2:358404/1‑251 (MQ=255) gTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCA‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCAccc > 1:598641/1‑250 (MQ=255) gCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCA‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGcc > 2:57547/1‑251 (MQ=255) gCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCA‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGcc > 1:494264/1‑251 (MQ=255) gtccccacccgTGCTGCTGGCGTTGGGGACCCCTGCGCCACCCCTATCAACATTCCTTTTCA‑GCTTTTTTTTCCTCCCAAGCGGGGTTTTCTCCCCACGATCCCCCGGACCCTCCCCTTCCGGCTTATTTT‑CTTCCATATCGGCCTCTAAATAGAAGGAGTG‑CTTTTCCACCACCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGgc < 1:289504/241‑1 (MQ=255) ccgtcCCGCGGGCTGCTGGCGTTGGGCCCCCCTGCGGCAACACTATCAACAGCCCCTTTCG‑CCACTTTGTTCCTGCCAAGCGGCGTGTTCTCTGCACGATCCCCCGGCCGCCCCCCTTCCAGCTTATTTT‑CTTCCATATGGGCCAGTACATAGAAGGAGTT‑TTTTTTCCCCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGgcgc < 1:428966/250‑1 (MQ=255) gtcACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCA‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGgcgcgc > 2:266592/1‑251 (MQ=255) ccccccgtgcggtgggtgTTGGTGCCCCCTGCGGCAACACTATCAACAGTCCCTTTCA‑GTTTTTTTTTTCTGCAAGGCGGCGTAACCTCTGCACGATCCCCCGGCCGCTCCCCTTCCAGCTTATTTT‑CTCCCATATAGGCCAGTACATAGAAGGATTT‑CTGTTCCCCCAGCAAAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGcgcgcg < 1:504023/234‑1 (MQ=255) cACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCA‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCgg > 2:48203/1‑251 (MQ=255) cgcgTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCA‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGAt > 2:515614/1‑251 (MQ=255) gctgGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCA‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGc > 1:416180/1‑251 (MQ=255) tgcggtgggTGCCCCTTGCGGCAACCCAATCAACCGTCCCTTCCC‑CCACTTTGTTACTGCCAAGCGGTGTGCCCTCTTCCAGACCCCCCGCACCCCCCCCTCCCGTCTTATTTT‑CTTCCAAATAGGCAATTACATAGAAGGTGTT‑CTGTTCCCACGGCCAAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGcn < 2:319429/243‑2 (MQ=255) ggggTTGGTGACCCCTGCGCAAACACTATCACCAG‑CCCCTTCACGCACTTTGTTACTGCCAAGCGGCGGGATCTCCGCACGATCCCCCGGACGCACCCCTCCCAGCTTATTTT‑CTTCCATATAGGCCAGTACATAAAAGGAGTT‑TTGTTTCCCCGGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGt < 2:416180/247‑1 (MQ=255) tGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCA‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCa > 1:194143/1‑248 (MQ=255) gtccccctgcgccaacctcataaccAGTCCCTTTCA‑GCACTTTGTTCCTGCCAACCGGGGTGCTCTCTCCCCAATCCCCCGGCCGCCCCCCTCCCAGCTAATTTC‑CTTCCATATAGCCCAGTACA‑AAAAGGGGTTTCTTTTCCCCCAGCGAAGCCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATcc < 2:362847/227‑1 (MQ=255) gACCCCTGCGGCAACACTATCAACAGTCCCTTTCA‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCa > 2:51524/1‑233 (MQ=255) gACCCCGGCGGCACCACTATCAACAGCCCCTTCCA‑GCCCTTTGTTACTGCAAAGCGGCGTGATCTCTCCACGATCCCCCGGACGCCCCCCTTCCAGCTTATTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTTTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCa < 1:51524/233‑1 (MQ=255) gggCAACACTATCAACAGTCCCTTTCA‑GCATTTTGTTACTGCCAGGCGGGGTGATCTCTACACGATACCCGGGACGCACCCCTTCCAGTTTATTTT‑CTTCCATATAGGCCATTACATAGAAGGAGTT‑CTTTTTCCCCAGCGAAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGcc < 2:420289/250‑1 (MQ=255) acTATCAACAGTCCCTTTCA‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGtaataa > 2:649519/1‑161 (MQ=255) aGTCCCTTTCA‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGccc > 2:35201/1‑250 (MQ=255) ggtactttccagaacttgggtACTGCGAAGGGGCGTGCTCTCTGCACGATCCCCCGGACGCCCCCCTTCAAGTTTAGTTC‑CCCCCATATAGGCCATCAAAAAGAAGGAGTT‑TGGTGTCCCCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACGCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCt < 1:480645/231‑1 (MQ=255) tCA‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTc > 1:93943/1‑251 (MQ=255) a‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGc > 1:654838/1‑251 (MQ=255) aCTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTa > 1:503798/1‑250 (MQ=255) tgttaatgcaaggcggagtaaTCTCTGCAAGATACCCCGGACGCCCCCCTTCAAGATTTTTTT‑CTTCCATATAGGCCAGTACAAAAAAGGAGTT‑CTTTTTAACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTg < 1:44934/230‑1 (MQ=255) cAAGCGGCGTGATCTCTGCACGATCCCCCGGACCCCCCCCTCCCAGCTTGTTTT‑CTTCAATATAGGCCAGTACAAAGAAGGAGTT‑TTTTTCCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAAcc < 2:194143/251‑1 (MQ=255) cAAGCGGCGTGATCTCTGCACGATACCCCGGACGCCCCCCTTCCAGTTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGATTT‑CTGTTTCCCCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAAcc < 1:57547/251‑1 (MQ=255) cAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAAcc > 2:413311/1‑251 (MQ=255) gcggcgTGATCTCTGCACGATCCCCCGGACGCCCCCCTTCCAGCTTATTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCa < 1:174322/128‑1 (MQ=255) gcggcgTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCa > 2:174322/1‑128 (MQ=255) cggcgTGTTCTCTGCACGATCCCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATGGGCCAGTACATAGAAGGCGTT‑CTGTTTCCCCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGttt < 1:431634/251‑1 (MQ=255) gTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTgt > 1:84378/1‑249 (MQ=255) gATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGATTgtagt > 2:33399/1‑250 (MQ=255) cccACGATCCCCCGGCCGCACCCCTTCCAGCTTATTTT‑CTTCCATATAGGCCATAACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACgttgt < 2:241306/249‑1 (MQ=255) gcagcatcccccGGACCCCCCCCTCCCAGTTTATTTT‑TTTCAAGATGGGCCAGTAAATAGAGGGAGTT‑TTTTTCCCCCAGCGCACCCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACgttgtt < 2:598641/243‑1 (MQ=255) cacgccaccccggcccccccccTTCCAGTTTATTTT‑CTTCCAAAGAGCCCAGGACATAGAAGGGTTT‑CTGTTGCACCAGGGCAGCCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACgttgtt < 1:393950/232‑1 (MQ=255) aCGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTc > 2:721438/1‑136 (MQ=255) aCCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTgg > 2:528405/1‑251 (MQ=255) ggACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGAt > 2:51532/1‑251 (MQ=255) cacaccccccCTCCAGCTTTGTTT‑CTTCCATATAGCCCATTAAATAAAAGTATTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGAtc < 2:100296/245‑1 (MQ=255) ccccTTCCAGCTTATTTT‑CTTCAATATAGCCAAGTACATAGAGGGAGTT‑CTTTTTCCCCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTcg < 1:346881/251‑1 (MQ=255) cccAGCTTATTTT‑TTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTTTTCCCCCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCgag < 1:611302/249‑1 (MQ=255) ccAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGAc > 1:383079/1‑251 (MQ=255) aGCTTATTTT‑CTTCAAAATGGCCCAGTACATAGAAGGAGTT‑CTGTTTCCCCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACAt < 1:33399/251‑1 (MQ=255) cTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCg > 1:78178/1‑251 (MQ=255) cTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATACTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGCTGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGGCGATCTCTTCGCGAGACATCg > 1:265156/1‑251 (MQ=255) gTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTg > 1:103493/1‑251 (MQ=255) gTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGCGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTg > 1:60559/1‑251 (MQ=255) ttt‑CTTCCATAGAGGCCATTACATAGAAGGAGTT‑TTTTTTCCCCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTg < 1:266592/250‑1 (MQ=255) cATATAGGCGAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCACGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCGCCGAGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGGCGCCTGAGCTTTCGCTAACTGAGGCAGAACCGTTTGTAGTACGGTGTTGGCCTGGGCGATCTCTTTGCGAGACATCGCCTGcgcgccgc > 2:259911/1‑244 (MQ=255) tataGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACgttgtt > 2:506854/1‑208 (MQ=255) tagagcccAGTACATAGAAGGAGTT‑CTGTTCCACCAGCGCATCCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACgttgtt < 1:506854/202‑1 (MQ=255) ataGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGAc > 2:718001/1‑251 (MQ=255) aCATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGCGACGGTTacgacg > 1:506856/1‑251 (MQ=255) gggAGTT‑CTTTTTCCCCGGCGCACCCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGcc < 2:317041/250‑1 (MQ=255) ggattt‑CTGTTTCCCCAGCACAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGcct < 2:222630/247‑1 (MQ=255) ggAGTT‑CTGTTTCCCCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGcct < 1:349435/251‑1 (MQ=255) ggAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGcct > 1:450459/1‑251 (MQ=255) aGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGATGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGcctcc > 1:71456/1‑251 (MQ=255) t‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTgg > 2:11539/1‑251 (MQ=255) cACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTtct > 2:120293/1‑251 (MQ=255) | TGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCA‑GCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTT‑CTTCCATATAGGCCAGTACATAGAAGGAGTT‑CTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCT > NC_000913/3388322‑3388819 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |