| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,261,055 | 0 | A | C | 28.4% | 209.1 / 12.4 | 96 | V455G (GTG→GGG) | tdcE | 2‑ketobutyrate formate‑lyase/pyruvate formate‑lyase 4 |
| Reads supporting (aligned to +/- strand): ref base A (57/11); new base C (0/27); total (58/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.15e-15 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTCGCCACCGACAGGCCCGCGATGCCGCATGCCATAGTGCGATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAATTCTTCCGACCAAAGAATGGTCAGGTTAGGTTCCGG > NC_000913/3260806‑3261305 | gTCGCCACCGACAGGCCCGCGATGCCGCATGCCATAGTGCGATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGAACTGAA‑TCTTCAGCTTCTCGTCCAc > 2:455434/1‑251 (MQ=255)gTCGCCACCGACAGGCCAGCGATGCCGCATGCCATAGCGCGATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCCGCTTCTCGTCCAc > 1:241021/1‑251 (MQ=255) cGCCACCGACAGGCCCGCGATGCCGCATGCCATAGTGCGATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCAccc > 1:550287/1‑251 (MQ=255) cACCGACAGGCCCGCGATGCCGCATGCCATAGTGCGATAGACATCACGATCGTGCAGCGCCAGCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCccgc < 1:105463/251‑1 (MQ=255) ccATGCCATGGTGCGATAAACATCACGAGCGGGCAGCCCAAGCGGCGAGGTTTGTTGGCTTTACTTGGCGTGCATGTAGGGAATGATATTCAGGGCGCTGATTTACTGCCCCGCCAGCCATTCCATGACGTGATCGGGGCGGTCCCTCCCTTTGTCGAAATCCTGCACGTCGCCCCCCAGCGG‑TGCTGTTTTCGG‑CCCGCCCTGAT‑TTGTCAGCTTCTCGCCCACCCCGCCGTTAATTGCGTAGAGCAGCg < 1:188722/250‑1 (MQ=255) ccATAGTGCGATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTc > 2:257675/1‑251 (MQ=255) aGTGCGATAGACTCCACGCTCGTGCGGCGCAATCAGCGAAGTTTCGTGGCTGTATTTGTCGTGCATGTAGTGAATGATTTTCAGCGCGCTGATGTACTGCCCCGCCAGCCAGTCCATGAAGTGATCGAGGCTTTCCATCTCTTTGTCGTAACCCAGCCCGTCTTCCATCAGGGG‑TGCTGTTTTCGG‑CCGGCCCTGAA‑TCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGcc < 1:287425/250‑1 (MQ=255) gTGCGATAGACATCACGATCGGGCAGCGCCATCAGCGAAGTTTGGTAGCTGTACTTGTCGTGCATGTAGTGAATGATTTTCAGCGCGCTGATGTACTGCCCCCCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTTTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAg < 1:524755/251‑1 (MQ=255) gATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGtt > 2:683438/1‑250 (MQ=255) gATAGACATCACAATCGTGCAGCGACATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGc > 1:530054/1‑226 (MQ=255) gATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAcc > 2:589488/1‑250 (MQ=255) gTGCAGCGCAATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaa > 1:108266/1‑248 (MQ=255) tGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGa > 2:679767/1‑250 (MQ=255) tGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTCCGCAGAGCAGCGTTTTCGCCAGGTTCGCGCGTGCACCAAAGa > 2:304222/1‑250 (MQ=255) gcagccccatgggggaagtttggTAGCTGTTCTTTTCGTTCATGTAGTGAATGATTTTCAGGGCTCTGATTTTCTCCCCCGCCAGCAAGTCCATGAAGTTATCGGGGCTGCCCATCCCTTTTTCGTAACCCAGCCCGTCGTCCATCAGGGG‑TGCTGTTTTCGG‑CCCG‑CCTGAAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAAc < 1:268367/229‑1 (MQ=255) gcgccatacgggAAGTTTCGTATTTGTACTTGTCGTGTATGTAGTGAATGATATTCAGCGCGCTGATGTCCTGCCCCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCCCTTTGTCGTAATCCAGCCCGTCGTCCATCAGCGG‑GGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCATCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGc < 1:470216/240‑1 (MQ=255) cAGCGAAGTTTCGTAGCTGTGCTTGTGGTGCATGTAGTGAATGATATTCGGCGCGTTGATGTACTGCCCCCCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCTCTTTGTCGTAATCCAGCACGTCGTCCACCAGCGG‑TGCTGTTTTCGG‑CCCGCCCTGAA‑TCTTCAGCTTCGCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCt < 1:16063/251‑1 (MQ=255) cAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCt > 2:742047/1‑251 (MQ=255) cAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCt > 1:396297/1‑251 (MQ=255) ccaccacaatccgcctggttcTTGTCGTGCGTGTGGTAAATGATATCCAGCGCGCTGTGGTACTGCACCGCCAGCCAGTCCATGAAGTGAGCGAGGCTGTCCTTCTCTTTGTCGTAATCCAGCACGTCGTCCTTCAGGGG‑TGCTGTTTTCGG‑CCCGACCTAAA‑TCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCt < 1:519864/230‑1 (MQ=255) aGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCtt > 2:253178/1‑251 (MQ=255) aGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATAAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAACGAACTGCATTTGCtt > 2:290733/1‑251 (MQ=255) ggAAGTTTCGTAGCTGTATTTGTCGTGCATGTGGTGAATGATTTTCAGGGCGCTGTTTTACTGCCCCCCCACCCAGTCCATGAAGTGATCGAGGCTGTCCATCTCTTTGTCGTAATCCAGCCCGCCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTAc < 1:364971/250‑1 (MQ=255) cGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTAc > 2:278855/1‑251 (MQ=255) gAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTAcc > 2:351141/1‑251 (MQ=255) aaGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTAcc > 2:166713/1‑250 (MQ=255) gCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAAt > 1:430112/1‑251 (MQ=255) ttGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGCGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGAc > 1:363395/1‑251 (MQ=255) tGTAGTGAATGATATTCAGCGCGCTGATGTACTGCCCCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGc > 2:557066/1‑250 (MQ=255) gTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATcg > 2:613166/1‑251 (MQ=255) gAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATcgcg > 1:652399/1‑251 (MQ=255) gAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATcgcg > 2:73486/1‑251 (MQ=255) atTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCGGTCCATAACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTACACCCCGCCGTTAATTGCGGAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCAtt > 2:583363/1‑220 (MQ=255) ttCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAAtcgtc > 1:493884/1‑251 (MQ=255) ggcgcgaatttcccccccccccagccagTCCATGAAGTGACCGGGGCTGCCCATCCTTTTTTCGTAACCCAGCCCGTCGTCCATCAGGGG‑TTTTGTTTTCGG‑CCCGACCGGAA‑TCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGt < 1:569958/231‑1 (MQ=255) cgcgcTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCTCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTTGCTGt > 2:382075/1‑251 (MQ=255) cgcgcTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGt > 1:271618/1‑251 (MQ=255) cgcTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTg > 1:589773/1‑251 (MQ=255) ggatttACTGCCCCGCCGTCCAGTCCATGAAGTGATGGAGGCTGTCCATCTTTTGGTCGTAACCCAGCACGTCGCCCATCAGGGG‑TGCTTTTTTGGG‑CCCGACCTGAA‑TCTTCACCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCGCCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAg < 1:732989/246‑1 (MQ=255) cccctccacgccgcttttccaatctGAAGTGATCGGGGCTGTCCATCTTTTTGTCGTAATCCAGCCCGTCGCCCATCGGGGG‑TGCTTTTTTCGG‑CCCGACCGGAA‑TCTTCATCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTc < 2:418626/226‑1 (MQ=255) gTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTc > 2:155796/1‑249 (MQ=255) ggccccccacgccatTCCAGGAAGGGATCGAGGCTGCCCATCGTTTGGTCTTAACCCAGACCGTCTTCCTCCGGCGT‑TGCTGTTTTCGC‑CCCGCCTTGAG‑TCTTCATCTTCTCTCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACg < 2:136417/241‑1 (MQ=255) cACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGc > 2:82490/1‑250 (MQ=255) ccGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGACAATCAGCGG‑TGCTGCTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGACGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATc > 1:644019/1‑251 (MQ=255) gccagccaGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATc > 1:565792/1‑249 (MQ=255) cagccagTCCAGGAAGTGCTCGAGGCTGTCCATCCCTTTGTCGTAACCCAGCACGTCGTCCACCAGCGG‑TGCTGTTTTCGG‑CCCGCCCTGAA‑TCTTCAGCTTCTCCTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGAt < 1:257675/251‑1 (MQ=255) caagTCAATGAAGTGATGGAGGCTGTCCATCCCTTTGTCGTAATCCAGCAGTTCGTCCATCAGCGG‑TGTTTTTTTCGG‑CCCGCCCTAAA‑TCTTCAGCTTCCCGCCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGt < 1:476299/249‑1 (MQ=255) cccaggaggTGACCGAGGCTGCCCATCACTTTGTCGTAACCCAGCCCGTCGTCCATCAGCGG‑TGTTTTTTTCGC‑CCCGCCCTGAA‑TCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCAGTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCAtt < 1:683805/243‑1 (MQ=255) ccATGAAGTGATCGAGGCTGTCCAACACTTTGTCGTAATCCAGAACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTCATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCCTTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCAttt > 2:274469/1‑251 (MQ=255) cATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCAtttt > 2:423494/1‑251 (MQ=255) gAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTc > 1:418626/1‑225 (MQ=255) aGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGt > 1:184976/1‑124 (MQ=255) aGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATAc > 1:352574/1‑251 (MQ=255) gATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGc > 2:587593/1‑251 (MQ=255) cGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCa > 1:499152/1‑249 (MQ=255) gAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGGTCGTCATTTTCATACTGCAAgg > 2:98268/1‑251 (MQ=255) ggCTGTCCATCACTTTGTCGGAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTTTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCCGACGCAGCAGGCAATAGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGa > 2:431674/1‑250 (MQ=255) tGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAag > 2:255858/1‑249 (MQ=255) ccATCTCTTTTTCGTAATCCAGCACGTCGTCCATCGGCGG‑TGCTTTTTTCGG‑CCCGACCGTAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTg < 1:475417/251‑1 (MQ=255) aTCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑CGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTATTCGTCGCTGGTGAAGGCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGAGAc > 2:470573/1‑251 (MQ=255) tCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACg > 1:681823/1‑251 (MQ=255) gTCGTAACCCACCCCGTCGTCCACCGGGGG‑TTCTTTTTTCGC‑CCCGCCCTGAT‑TCTCCAGCTTCCCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGac < 1:82490/250‑1 (MQ=255) tCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGacac > 2:89593/1‑251 (MQ=255) cGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACAcc > 2:47815/1‑251 (MQ=255) aaTCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTgc > 1:642044/1‑251 (MQ=255) cccAGCCCGTCGTCCA‑CCGCGGGTGCTGTTTTCGG‑CCCGCCCTGAA‑TCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTgcgc < 1:89593/250‑1 (MQ=255) cccccacgccccccaccggCGG‑TGCTGTTTTCGG‑CCCGCCCTGAC‑TCTCCACCTTCTCCCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGcgcg < 2:449572/233‑1 (MQ=255) gCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGGGACGATCGACACCTGCGCGGc > 2:4036/1‑250 (MQ=255) ncgtcgtCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCat > 2:328940/2‑250 (MQ=255) aCGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCat > 2:171379/1‑250 (MQ=255) cgtcCATCGGCGG‑TGCGTTTTTCGG‑CCCGCCCTGAA‑TCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATAtttt < 1:324397/251‑1 (MQ=255) cccccccactcGG‑TGCTTTTTTCGC‑CCCG‑CCCGAACTCTTCAGCTTCTCCCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATAtttt < 2:1925/241‑1 (MQ=255) cccttcagctgttgtttttcccg‑CCCGACTTGAT‑TCTGCACCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATAtttt < 2:708568/227‑1 (MQ=255) cgtgtgctctcccgatcgcGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGa < 1:389516/233‑1 (MQ=255) cgaGCGG‑TGCTTTTTTGGC‑CCCGCCCTGAA‑TCTTCAGCCTCTCTTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAg < 1:566676/249‑1 (MQ=255) aGCGG‑TGCTGTTTTCGG‑CCCGACCTGAT‑TCTTCAGCTTCTCGCCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAgg < 1:9533/250‑1 (MQ=255) aGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAgg > 2:9533/1‑250 (MQ=255) g‑TGCTGTTTTCGA‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATc > 1:518708/1‑251 (MQ=255) gtgttttttCGGGCCCGCCCGGAA‑TCTTCATCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATc < 1:679767/246‑1 (MQ=255) ggCTGTTTCCGC‑CCCGCCCTGAA‑TCTTCTCCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCg < 1:278855/250‑1 (MQ=255) gTTTTCGG‑CCCGCCCTAAA‑TCTTCAGCTTCCCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTaa < 1:587593/251‑1 (MQ=255) gTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTa > 1:613515/1‑250 (MQ=255) gcGG‑CCCGCCCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAAttc < 1:171379/249‑1 (MQ=255) ccgcccggACCTGAT‑TCTTCACGTTCGCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAAttc < 2:558804/243‑1 (MQ=255) cGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGa > 2:389516/1‑233 (MQ=255) gg‑CCCGACCTGAA‑TCTTCACCTCCTCGCCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAAttcttc < 1:589207/251‑1 (MQ=255) cgccccGACCTGAT‑TCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAAttctt < 1:166713/248‑1 (MQ=255) gtccgacccTGAT‑TCTTCCGCTTCTCTCCCACCCCTCCGTTAAGTGCGTAGAGCAGCGTTTTCGCAAGTTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCGCCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGGTGAAGTCAGTACGCATCAGATCGTGATGTTCATACTGCAAGGAAGAGGTGACGATCGACGCCTGCGCGGCATATTTTTTGAAGGCAATCGGTAAttcttc < 2:648903/244‑1 (MQ=255) ccccgccttatctctacatcTTCTCCTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAATTCTTcc < 2:652399/232‑1 (MQ=255) gACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAATTCTTCCGAcc > 2:88885/1‑251 (MQ=255) tGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAATTCTTCCGACCaa > 1:674100/1‑249 (MQ=255) cTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGACAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAATTCTTCCGACCAAAGAATGGTCaggt > 2:229564/1‑251 (MQ=255) tctcGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAATTCTTCCGACCAAAGAATGGTCaggtt < 1:642817/250‑1 (MQ=255) tctcGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAATTCTTCCGACCAAAGAATGGTCaggtt < 1:73486/250‑1 (MQ=255) cGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAATTCTTCCGACCATAGAATGGTCaggttag < 1:351141/249‑1 (MQ=255) cACCCCGCCGTTAATTGCGTAGAGAAGCGTTTTCGCAAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAATTCTTCCGACCAAAGAATGGTCAGGTTAGGTTCCg > 1:594334/1‑251 (MQ=255) aCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAATTCTTCCGACCAAAGAATGGTCAGGTTAGGTTCCgg > 1:439331/1‑251 (MQ=255) | GTCGCCACCGACAGGCCCGCGATGCCGCATGCCATAGTGCGATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGG‑TGCTGTTTTCGG‑CCCGACCTGAA‑TCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAATTCTTCCGACCAAAGAATGGTCAGGTTAGGTTCCGG > NC_000913/3260806‑3261305 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |