Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,386,8620GA30.3% 59.4 / 11.0 34intergenic (+31/‑72)yfbL/yfbMputative peptidase YfbL/DUF1877 domain‑containing protein YfbM
Reads supporting (aligned to +/- strand):  ref base G (1/22);  new base A (10/0);  total (12/22)
Fisher's exact test for biased strand distribution p-value = 1.19e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCC‑GCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGC‑GACATGCACA‑TTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACT‑GAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTTACACTTTGGGCTGACAGGCACCTCTGCTT  >  NC_000913/2386620‑2387108
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                         
cTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCC‑GCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GCCAGAAAAAAC‑GAAATGc                                                                                                                                                                                                                                                  >  1:634918/1‑251 (MQ=255)
       tACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCC‑GCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGCTCCGGAAAAA‑C‑GACagtaaaatttt                                                                                                                                                                                                                                           >  1:395296/1‑241 (MQ=255)
          cccAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCC‑GCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GCCAGAAAAAAC‑AACATGCACa‑atttttt                                                                                                                                                                                                                                       >  1:534673/1‑248 (MQ=255)
                          gtttttgtttcctctGCCCACCTGAATTTTGGGAAACCCTATATCCCC‑GCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGC‑GACATGCACA‑TTGTATTTTCCCTCATCTGtttt                                                                                                                                                                                                                       <  1:164123/240‑1 (MQ=255)
                                            caccTGAATTATTGGCAACACGATATTCCC‑GCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GTCAGAAAAAAA‑GACATGCACA‑ATTTTTTTTTCCTCCTCTTTTTTAAGATGCGGAACAtaaa                                                                                                                                                                                                      >  1:700408/1‑247 (MQ=255)
                                                 aatttattGACAACACGA‑ATCCCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGC‑GACATGCACA‑TTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCaa                                                                                                                                                                                                <  2:91747/248‑1 (MQ=255)
                                                  aaTTATGGGAAACACTATTTTCCC‑GCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGC‑GACATGCACA‑TTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAAc                                                                                                                                                                                               <  1:503375/251‑1 (MQ=255)
                                                     tatcggaaaCACGATATTCCC‑GCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGC‑GACATGCACA‑TTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACgg                                                                                                                                                                                             <  1:348377/243‑1 (MQ=255)
                                                              cagtacctcccc‑GCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGC‑GACATGCACA‑TTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAaaata                                                                                                                                                                                   <  2:667420/242‑1 (MQ=255)
                                                               acGATATTCCC‑GCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GCCAGAAAAAACAGA‑AAGCACA‑TTTTTTTTTTCCTCATCTGTTTTTAGATGCGAAAAAAGAAATTTAACGGAGGaaaaaaa                                                                                                                                                                                   >  1:257559/1‑248 (MQ=255)
                                                                  atatTCCC‑GCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCACAAAAAAA‑GACAATCACA‑ATTTTTTTTTTCTCCTCTGTTTTTAGATGCGAAACAAGAAATTTTACAGAGGAAaaaaaaatt                                                                                                                                                                               >  2:492025/1‑250 (MQ=255)
                                                                         c‑GCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TGCAGAAAAAAA‑GACAAGCACA‑TTTTTTTTTTTCTCCTCTGTTTTTATAAGCGAAACATGAAATTTAACAGGGGAAAAATAAATGGGGATg                                                                                                                                                                         >  1:335051/1‑250 (MQ=255)
                                                                                   gATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGTG‑TCCAGAAAAAGC‑GACATGCACA‑TTTTATTTTCCCTCATCTGTGTTAAGATGCGAAACATGAAGTTCAACGGAGGAAAAATAAATGGGAATGATTGGCTAt                                                                                                                                                                <  2:243806/250‑1 (MQ=255)
                                                                                              ccGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGC‑GACATGCACA‑TTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAAtt                                                                                                                                                    <  2:225961/251‑1 (MQ=255)
                                                                                                   ttttATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGC‑GACATGCACA‑TTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGATGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGAc                                                                                                                                                 <  1:556512/249‑1 (MQ=255)
                                                                                                               aaaCAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGC‑GACATGCACA‑TTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTa                                                                                                                                    <  1:394771/250‑1 (MQ=255)
                                                                                                                                           cagacagaTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGC‑GACATGCACA‑TTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACT‑GAAAAACCt                                                                                                      <  2:440324/251‑1 (MQ=255)
                                                                                                                                              acagaTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCCGAAAAAAC‑GACATGCACA‑TTTTTTTTTTCCCCCTCTTTTTTTAAATGCGAAACAAGAAAATTAAAGGAGGGAAAAAAAATTGGGATGATTTGGTTTTTTTCTGAAATTTAATCAAAAAAGATTAAACAACTGCTTGAGTCTACT‑GAAAAAACtttt                                                                                                   >  1:546680/1‑250 (MQ=255)
                                                                                                                                                             aGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGC‑GACATGCACA‑TTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACT‑GAAAAACCTTTGATGGATAATATACAt                                                                                    <  1:458694/251‑1 (MQ=255)
                                                                                                                                                              gAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGC‑GACATGCACA‑TTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACT‑GAAAAACCTTTGATGGATAATATACATg                                                                                   <  2:534673/251‑1 (MQ=255)
                                                                                                                                                                     gCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGC‑GACATGCACA‑TTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACT‑GAAAAACCTTTGATGGATAATATACATGACACGCt                                                                            <  2:254364/251‑1 (MQ=255)
                                                                                                                                                                              gTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCCGCAAAAAA‑GACAAGAACA‑TTTTTTTTTTCCTCATATGTTTTTATAAGAGAAAAATGAAAATATACTGGGGAAAAAAAAAAGGGAATGATTGGCTCTTTTGCACAAATTGAATCAGAAAAAATTAAACAAATGCTTGCGTCTACT‑GAAAAAACTTTTATGGAATATATACATGACACGcattttgtatt                                                                   >  1:318151/1‑241 (MQ=255)
                                                                                                                                                                                ggATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGC‑GACATGCACA‑TTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACT‑GAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTAc                                                                 <  2:727467/251‑1 (MQ=255)
                                                                                                                                                                                    ggTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGC‑GACATGCACA‑TTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACT‑GAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGcc                                                              <  1:492025/250‑1 (MQ=255)
                                                                                                                                                                                        ttATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GTCAGAAAAAAG‑GAAAAGGACA‑TTTTATTTTTTCTCCTCTGTTTTTAGAAGCGGAAAAAGAAAATTAACGGGGGGAAAAAAAATGGGGATGATTGGGTATTTTTTTGAAAATGGCTCAGAAAAAATTAAAAAACTGCTTGAGTATACTCAAAAAAACTTT‑ATGGATAATAAACAAAACACGCTTTctgtgatacaccgactg                                                         >  2:694262/1‑235 (MQ=255)
                                                                                                                                                                                          ataACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGC‑GACATGCACA‑TTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACT‑GAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGa                                                       <  2:634918/251‑1 (MQ=255)
                                                                                                                                                                                           taACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GCCACAAAAAAC‑GCCAAGCACAATTTTTTTTTCCC‑CATCTCTTTTTAAAAGCGGAAAATTAAAATTAACGGAGGGAAAATAAATGGGGATGATTGGGTATTTTTTAGAAAATGACTCAGAAAAAATTAAACAAATACTTGTGTCTACT‑GAAAAAACCTTGATTGATAATATACACGACACGCTTTCCGGATTAAGcccaccgga                                                       >  1:667311/1‑243 (MQ=255)
                                                                                                                                                                                                ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGC‑GACATGCACA‑TTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACT‑GAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATAtt                                                   <  2:89663/249‑1 (MQ=255)
                                                                                                                                                                                                        aaacaGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGC‑GACATGCACA‑TTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACT‑GAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCt                                         <  1:611712/250‑1 (MQ=255)
                                                                                                                                                                                                                        aaaTTTCTCGAGGCGGG‑TCCAGAAAAAGC‑GACATGCACA‑TTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACT‑GAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTTACACtt                         <  2:547774/251‑1 (MQ=255)
                                                                                                                                                                                                                                       gg‑TCCAGAAAAAGC‑GACATGCACA‑TTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACT‑GAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTTACACTTTGGGCTGACAGGCAc          <  1:555320/251‑1 (MQ=255)
                                                                                                                                                                                                                                           ccAGAAAAAGC‑GACATGCACA‑TTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACT‑GAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTTACACTTTGGGCTGACAGGCACctn       <  2:335051/251‑2 (MQ=255)
                                                                                                                                                                                                                                            cAGAAAAAGC‑GACATGCACA‑TTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACT‑GAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTTACACTTTGGGATGACAGGCACctct      <  2:395296/251‑1 (MQ=255)
                                                                                                                                                                                                                                                aaaaGC‑GACATGCACA‑TTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACT‑GAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTTACACTTTGGGCTGACAGGCACCTCTGCtt  >  1:462348/1‑251 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                         
CTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCC‑GCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGC‑GACATGCACA‑TTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACT‑GAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTTACACTTTGGGCTGACAGGCACCTCTGCTT  >  NC_000913/2386620‑2387108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑