| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,512,183 | T→C | E54E (GAA→GAG) | yhfZ ← | putative DNA‑binding transcriptional regulator YhfZ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,512,183 | 0 | T | C | 97.5% | 144.9 / ‑6.1 | 40 | E54E (GAA→GAG) | yhfZ | putative DNA‑binding transcriptional regulator YhfZ |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (24/15); total (24/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAGGCCTTCGTACAAACGGGTATAGGGCAAGGGCATTGCACATACCACGTTGTTGATATCCACATGAGTCAGCAATGCTTTGTTATCCATCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCG > NC_000913/3511937‑3512423 | gccagagaattctggccttcttaatgactctttttatatattcagcaaataaaacatagccccttaataaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCa < 1:371340‑M2/62‑1 (MQ=255) ggccttcttaatgactctttttatatattcagcaaataaaacatagccccttaataaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAAc < 1:478890‑M2/73‑1 (MQ=255) gccttcttaatgactctttttatatattcagcaaataaaacatagccccttaataaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGCCTCCAGCGTTTTCAAcgcg > 2:161943‑M2/176‑251 (MQ=255) ctttttatatattcagcacataaaacatagccccttaataaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGcc > 2:410828‑M2/161‑250 (MQ=255) atattcagcaaataaaacgtagccccttaataaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGAGTCAGGCCAAcggc > 2:398968‑M2/153‑247 (MQ=255) gccccttaataaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTc > 1:326447‑M2/132‑251 (MQ=255) ccccttaataaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCtct > 1:339942‑M2/131‑250 (MQ=255) ttaataaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCt > 1:112777‑M2/127‑250 (MQ=255) atctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAAt < 1:473243‑M2/136‑1 (MQ=255) atctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATc > 1:124388‑M2/115‑251 (MQ=255) tctgctaatgggttatatgcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCg > 2:410289‑M2/114‑251 (MQ=255) gttatattcaccgttacatcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCACTCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGTCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACt > 2:413752‑M2/103‑251 (MQ=255) acagctacttacaaaattaaggcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCGCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAAt < 2:12572‑M2/164‑1 (MQ=255) taagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTc < 1:466190‑M2/133‑1 (MQ=255) taagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGcgtcgt < 1:366274‑M2/180‑1 (MQ=255) taagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCAAGCTCATCTATGGTTTTc > 2:466190‑M2/71‑203 (MQ=255) cgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATgacgac < 1:222239‑M2/189‑1 (MQ=255) cgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGacgacg > 1:397157‑M2/62‑251 (MQ=255) ctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGcc > 1:232753‑M2/60‑251 (MQ=255) cactgacaccaattagatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATgac < 1:394791‑M2/186‑1 (MQ=255) cactgacaccaattagatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGATTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATgac > 2:394791‑M2/58‑243 (MQ=255) actccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattaCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACTCCTTCtttt > 2:73390‑M2/53‑251 (MQ=255) gccaattaaatcttcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACTGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACGTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTGCtttttt < 2:159875‑M2/201‑1 (MQ=255) atattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACACAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGt > 2:411675‑M2/42‑251 (MQ=255) attcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTc < 1:288564‑M2/212‑1 (MQ=255) tgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCTTCAGGCCAACGGATGAAc < 2:406155‑M2/101‑1 (MQ=255) tgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAAc > 1:406155‑M2/35‑135 (MQ=255) tgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGc > 1:167612‑M2/35‑251 (MQ=255) tcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGCCGCata > 2:49539‑M2/31‑250 (MQ=255) gtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAAt > 1:38833‑M2/15‑251 (MQ=255) tcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTg < 1:211655‑M2/239‑1 (MQ=255) gtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTACATTGAat > 2:297745‑M2/9‑250 (MQ=255) cAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAgg > 1:70927/1‑251 (MQ=255) ccATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGa < 1:297745/251‑1 (MQ=255) cATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCCCTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGCCGACGCCTTCTTTTTTGATAAACGTTCGACGCCTAATCAATTTTCAGTAAANTGCATATTTATATCTTCAGGAATTTGAAGa > 2:210039/1‑250 (MQ=255) cccACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGgcgc > 1:364345/1‑251 (MQ=255) aCGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAAc < 1:410828/251‑1 (MQ=255) gTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCg > 1:10738/1‑250 (MQ=255) aTCGCGCCTCTTGATGCCAGCGTTTTCAACGCGGCCTGCTTCAGGCCAACGTATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCATTAAATTGAATATTTATATCTTCAGGAATTTGAATATAAGGCGCAACAGCGAGATGTGGAAAc < 1:258807/251‑1 (MQ=255) ccGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCg < 1:411675/249‑1 (MQ=255) | ATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAGGCCTTCGTACAAACGGGTATAGGGCAAGGGCATTGCACATACCACGTTGTTGATATCCACATGAGTCAGCAATGCTTTGTTATCCATCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCG > NC_000913/3511937‑3512423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |