Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,499,391	G→T	intergenic (+190/‑57)	cysG → / → yhfL	siroheme synthase/DUF4223 domain‑containing lipoprotein YhfL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,499,391	0	G	T	100.0%	151.6 / NA	40	intergenic (+190/‑57)	cysG/yhfL	siroheme synthase/DUF4223 domain‑containing lipoprotein YhfL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (25/15);  total (25/15)

ATTGGTCGGGTTGTTGGCCTGCGCGATAAACTGAACTGGTTCTCCAACCATTAATTTAACCCGGGCCAGAGAATTCTGGCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTGCTTATATTCATATAATCAATGAATATTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTC  >  NC_000913/3499148‑3499620                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                      attGGTCGGGTTGTTGGCCTGCGCGATAAACTGAACTGGTTCTCCAACCATTAATTTAACCCGGGCCAGAGAATTCTGGCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTatat                                                                                                                                                                                                                                <  2:22604/251‑1 (MQ=255)    ggTCGGGTTGTTGGCCTGCGCGATAAACTGAACTGGTTCTCCAACCATTAATTTAACCCGGGCCAGAGAATTCTGGCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCc                                                                                                                                                                                                                             >  1:413872‑M1/1‑250 (MQ=255)        gggTTGTTGGCCTGCGCGATAAACTGAACTGGTTCTCCAACCATTAATTTAACCCGGGCCAGAGAATTCTGGCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcga                                                                                                                                                                                                                         <  1:466151‑M1/251‑6 (MQ=255)                          aTAAACTGAACTGGTTCTCCAACCATTAATTTAACCCGGGCCAGAGAATTCTGGCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACCTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgc                                                                                                                                                                                                       >  1:42300‑M1/1‑228 (MQ=255)                                tgaactgGTTCTCCAACCATTAATTTAACCCGGGCCAGAGAATTCTGGCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccac                                                                                                                                                                                                 >  1:395818‑M1/1‑222 (MQ=255)                                   actgGTTCTCCAACCATTAATTTAACCCGGGCCAGAGAATTCTGGCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTCACGAGCAGTCTTCGTATATTCctcgaccagatcactgccattgcgcccacggc                                                                                                                                                                                              >  2:458342‑M1/1‑219 (MQ=255)                                     tgGTTCTCCAACCATTAATTTAACCCGGGCCAGAGAATTCTGGCATTCTTAATGACTCTTTTTATATATTCATCAAATAAAACATAGCCCCTGAATAAATATTAGCTGCTAAGGGGTTATATTCACCGTTCCAGCGACTTACAAAATTAAGTCCGTCGCGCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagatagctgccatggcgccctcggct                                                                                                                                                                                             <  2:409352‑M1/250‑34 (MQ=255)                                       gTTCTCCAACCATTAATTTAACCCGGGCCAGAGAATTCTGGCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATATCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttc                                                                                                                                                                                          >  1:159875‑M1/1‑215 (MQ=255)                                                  aTTAATTTCACCCGGGCCAGAGAATTCTGGCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGATAATGGTTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcactccgtatcg                                                                                                                                                                               >  2:325540‑M1/1‑204 (MQ=255)                                                                 gCCAGAGAATTCTGGCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactcca                                                                                                                                                                <  1:371340‑M1/251‑63 (MQ=255)                                                                              ggCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaac                                                                                                                                                     <  1:478890‑M1/249‑74 (MQ=255)                                                                               gCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgcctccagcgttttcaacgcg                                                                                                                                                  >  2:161943‑M1/1‑175 (MQ=255)                                                                                              ctTTTTATATATTCAGCACATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggcc                                                                                                                                    >  2:410828‑M1/1‑160 (MQ=255)                                                                                                      atatTCAGCAAATAAAACGTAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgagtcaggccaacggc                                                                                                                              >  2:398968‑M1/1‑152 (MQ=255)                                                                                                                           gCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctc                                                                                                      >  1:326447‑M1/1‑131 (MQ=255)                                                                                                                            ccccTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctct                                                                                                     >  1:339942‑M1/1‑130 (MQ=255)                                                                                                                                ttAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatct                                                                                                  >  1:112777‑M1/1‑126 (MQ=255)                                                                                                                                            atCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatc                                                                                     >  1:124388‑M1/1‑114 (MQ=255)                                                                                                                                            atCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaat                                                                                      <  1:473243‑M1/250‑137 (MQ=255)                                                                                                                                             tCTGCTAATGGGTTATATGCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcg                                                                                    >  2:410289‑M1/1‑113 (MQ=255)                                                                                                                                                        gTTATATTCACCGTTACATCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcactccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacgtcattcatttgccagctcatctatggttttcaatcgattaccgcact                                                                         >  2:413752‑M1/1‑102 (MQ=255)                                                                                                                                                               tCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactc                                                                                                                                                                  <  1:121670‑M1/155‑61 (MQ=255)                                                                                                                                                               tCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactc                                                                                                                                                                  >  2:121670‑M1/1‑95 (MQ=255)                                                                                                                                                                       aCAGCTACTTACAAAATTAAGGCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCcgcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaat                                                          <  2:12572‑M1/251‑165 (MQ=255)                                                                                                                                                                                        tAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgt                                          <  1:366274‑M1/250‑181 (MQ=255)                                                                                                                                                                                        tAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttc                                                                                         <  1:466190‑M1/203‑134 (MQ=255)                                                                                                                                                                                        tAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgcaagctcatctatggttttc                                                                                         >  2:466190‑M1/1‑70 (MQ=255)                                                                                                                                                                                                 cGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtgatgacgacg                                >  1:397157‑M1/1‑61 (MQ=255)                                                                                                                                                                                                 cGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtgatgacgac                                 <  1:222239‑M1/250‑190 (MQ=255)                                                                                                                                                                                                   cTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtgatgacgacgcc                              >  1:232753‑M1/1‑59 (MQ=255)                                                                                                                                                                                                     cACTGACACCAATTAGATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtgatgac                                    <  1:394791‑M1/243‑187 (MQ=255)                                                                                                                                                                                                     cACTGACACCAATTAGATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggatttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtgatgac                                    >  2:394791‑M1/1‑57 (MQ=255)                                                                                                                                                                                                          actccaatTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTActcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtgatgacgactccttctttt                       >  2:73390‑M1/4‑52 (MQ=255)                                                                                                                                                                                                            gCCAATTAAATCTTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaactgcattcatttgccagctcatctatggttttcaatcgattaccgcacgtttcacccaacaaataacgggccagcgtcgtgatgacgacgcctgctttttt                     <  2:159875‑M1/251‑202 (MQ=255)                                                                                                                                                                                                                     atatTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacacaacaaataacgggccagcgtcgtgatgacgacgccttcttttttgataaacgt            >  2:411675‑M1/1‑41 (MQ=255)                                                                                                                                                                                                                       atTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtgatgacgacgccttcttttttgataaacgttc          <  1:288564‑M1/251‑213 (MQ=255)                                                                                                                                                                                                                            tGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcttcaggccaacggatgaac                                                                                                                         <  2:406155‑M1/135‑102 (MQ=255)                                                                                                                                                                                                                            tGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtgatgacgacgccttcttttttgataaacgttcgacgc     >  1:167612‑M1/1‑34 (MQ=255)                                                                                                                                                                                                                            tGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaac                                                                                                                         >  1:406155‑M1/1‑34 (MQ=255)                                                                                                                                                                                                                                tCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtgatgacgacgccttcttttttgataaacgttcgccgcata  >  2:49539‑M1/1‑30 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                      ATTGGTCGGGTTGTTGGCCTGCGCGATAAACTGAACTGGTTCTCCAACCATTAATTTAACCCGGGCCAGAGAATTCTGGCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTGCTTATATTCATATAATCAATGAATATTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTC  >  NC_000913/3499148‑3499620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑