Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,421,374	C→T	S17L (TCA→TTA)	yhdY →	putative ABC transporter membrane subunit YhdY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,421,374	0	C	T	100.0%	125.8 / NA	37	S17L (TCA→TTA)	yhdY	putative ABC transporter membrane subunit YhdY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/21);  total (16/21)

ACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCAACGCGTGCCGACTGCACAAAAGCCGGTGCCTGTTGGGTCTTCATCCACGAACGATTTGGTCAGTTTATGTATGGGCTTTACCCACACGACCAACGCTGGC  >  NC_000913/3421128‑3421619                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      atgtgaatgacctctatgagctggtactggctgaagtagaacagcccctgttggacatggtgatgcaatacacccgtggtaaccagacccgtgctgcgctgatgatgggcatcaaccgtggtacgctgcgtaaaaaattgaaaaaatacggcatgaactaattcaggttagctaaatgcttgattaaaaaggcgatactcggcatggggaagcgccttttttataggtgtcacaaaggaCATAACTTAAGc                                                                                                                                                                                                                                                   >  1:471346‑M2/240‑251 (MQ=255)   gtgaatgacctctatgagctggtactggctgaagtagaacagcccctgttggacatggtgatgcaatacacccgtggtaaccagacccgtgctgcgctgatgatgggcatcaaccgtggtacgctgcgtaaaaaattgaaaaaatacggcatgaactaattcaggttagctaaatgcttgattaaaaaggcgctactcggcatggggaagcgccttttttataggtgtcacaaaggaCATAACTTAAGcc                                                                                                                                                                                                                                                  <  1:364197‑M2/13‑1 (MQ=255)         gacctctatgagctggtactggctgaagtagaacagcccctgttggacatggtgatgcaatacacccgtggtaaccagacccgtgctgcgctgatgatgggcatcaaccgtggtacgctgcgtaaaaaattgaaaaaatacggcatgaactaattcaggttagctaaatgcttgattaaaaaggcgctactcggcatggggaagcgccttttttataggtgtcacaaaggaCATAACTTAAGCCGCGCGAt                                                                                                                                                                                                                                           >  1:300644‑M2/232‑251 (MQ=255)                atgagctggtactggctggagtagaacagcccctgttggacatggtgatgcaatacacccgtggtaaccagacccgtgctgcgctgatgatgggcatcaaccgtggtacgctgcgtaaaaacttgaaaaaatacggcatgaactaattcaggttagctaaacgcttgattacaaaggcgctactcggcatggggaagcgccttttttataggtttaaaaaaggaCCTAACTTTAGCCGCGCGATGGTGTgg                                                                                                                                                                                                                                    >  2:398948‑M2/225‑251 (MQ=255)                    gctggtactggctgaagtagaacagcccctgttggacatggtgatgcaatacacccgtggtaaccagacccgtgctgcgctgatgatgggcatcaaccgtggtacgctgcgtaaaaaattgaaaaaatacggcatgaactaattcaggttagctaaatgcttgattaaaaaggcgctactcggcatggggaagcgccttttttataggtgtcacaaaggaCATAACTTAAGCCGCGCGATGGTGTGGGTGc                                                                                                                                                                                                                                <  1:213818‑M2/31‑1 (MQ=255)                          actggctgaagtagaacagcccctgttggacatggtgatgcaatacacccgtggtaaccagacccgtgctgcgctgatgatgggcatcaaccgtggtacgctgcgtaaaaaattgaaaaaatacggcatgaactaattcaggttagctaaatgcttgattaaaaaggcgctactcggcatggggaagcgccttttttataggtgtcacaaaggaCATAACTTAAGCCGCGCGATGGTGTGGGTGCGaaaaa                                                                                                                                                                                                                          <  1:189321‑M2/37‑1 (MQ=255)                                             cccctgttggacatggtgatgcaatacacccgtggtaaccagacccgtgctgcgctgatgatgggcatcaaccgtggtacgctgcgtaaaaaattgaaaaaatacggcatgaactaattcaggttagctaaatgcttgattaaaaaggcgctactcggcatggggaagcgccttttttataggtgtcacaaaggaCATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAg                                                                                                                                                                                                       <  1:198869‑M2/56‑1 (MQ=255)                                                                           cgtggtaaccagacccgtgctgcgctgatgatgggcatcaaccgtggtacgctgcgtaaaaaattgaaaaaatacggcatgaactaattcaggttagctaaatgcttgattaaaaaggcgctactcggcatggggaagcgccttttttataggtgtcacaaaggaCATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATg                                                                                                                                                                         >  1:125341‑M2/166‑251 (MQ=255)                                                                              ggtaaccagacccgtgctgcgctgatgatgggcatcaaccgtggtacgctgcgtaaaaaattgaaaaaatacggcatgaactaattcaggttagctaaatgcttgattaaaaaggcgctactcggcatggggaagcgccttttttataggtgtcacaaaggaCATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGtt                                                                                                                                                                      <  2:377595‑M2/89‑1 (MQ=255)                                                                              ggtaaccagacccgtgctgcgctgatgatgggcatcaaccgtggtacgctgcgtaaaaaattgaaaaaatacggcatgaactaattcaggttagctaaatgcttgattaaaaaggcgctactcggcatggggaagcgccttttttataggtgtcacaaaggaCATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGtt                                                                                                                                                                      >  1:176037‑M2/163‑251 (MQ=255)                                                                                 aaccagacccgtgctgcgctgatgatgggcatcaaccgtggtacgctgcgtaaaacattgaaaaaatacggcatgaactaattcaggttagctaaatgcttgattaaaaaggcgctactcggcatggggaagcgccttttttataggtgtcacaaaggaCATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGACTGCTGACTATTGGCTGCCTATGGTTGAt                                                                                                                                                                   >  1:419914‑M2/160‑251 (MQ=255)                                                                                 aaccagacccgtgctgcgctgatgatgggcatcaaccgtggtacgctgcgtaaaaaattgaaaaaatacggcatgaactaattcaggttagctaaatgcttgattaaaaaggcgctactcggcatggggaagcgccttttttataggtgtcacaaaggaCATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGAt                                                                                                                                                                   <  1:332079‑M2/92‑1 (MQ=255)                                                                                           gtgctgcgctgatgatgggcatcaaccgtggtacgctgcgtaaaaaattgaaaaaatacggcatgaactaattcaggttagctaaatgcttgattaaaaaggcgctactcggcatggggaagcgccttttttataggtgtcacaaaggaCATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTg                                                                                                                                                         >  1:78689‑M2/150‑251 (MQ=255)                                                                                             gctgcgctgatgatgggcatcaaccgtggtacgctgcgtaaaaaattgaaaaaatacggcatgaactaattcaggttagctaaatgcttgattaaaaaggcgctactcggcatggggaagcgccttttttataggtgtcacaaaggaCATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGAt                                                                                                                                                       <  2:317027‑M2/104‑1 (MQ=255)                                                                                                                         gtacgctgcgtaaaaaattgaaaaaatacggcatgaactaattcaggttagctaaatgcttgattaaaaaggcgctactcggcatggggaagcgccttttttataggtgtcacaaaggaCATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTTCTGAACTGGGCATTTTTg                                                                                                                           >  2:7368‑M2/120‑251 (MQ=255)                                                                                                                           acgctgcgtaaaaaattgaaaaaatacggcatgaactaattcaggttagctaaatgcttgattaaaaaggcgctactcggcatggggaagcgccttttttataggtgtcacaaaggaCATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGc                                                                                                                          <  2:426603‑M2/133‑1 (MQ=255)                                                                                                                                gcgtaaaaaattgaaaaaatacgtcatgaactaatgcaggttagctaaatgcttgattaaaaaggcgctacgcggcatggggaagcgccttttttataggtgtcacaaaggaCATAACTTAAGCCGCGCGATGGTGTGGGCGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATGGGCTGCATATGGTTGATGTGGGAACTGGTCCCACCTTTGCTGAACTGGGCATTTTTGCATGCt                                                                                                                     <  2:420071‑M2/138‑1 (MQ=255)                                                                                                                                gcgtaaaaaattgaaaaaatacggcagggactaattcaggttagataaatgcttgattcaaaaggcgctactcggcatggggaagcgccttttttataggggacacaaaggaCATAAATTAAGCAGCGCGATGGTGTGGGGGCGAAAAAATATGTTCTCCCGCTGGGGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGCTGTGGGCACTGATCCCACCGTTGCTGTACTGGGCATTTTTGcaggat                                                                                                                     >  2:426110‑M2/113‑248 (MQ=255)                                                                                                                                               aaaatacggcatgaactaattcaggttagctaaatgcttgattaaaaaggcgctactcggcatggggaagcgccttttttataggtgtcacaaaggaCATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCa                                                                                                      >  1:183409‑M2/98‑250 (MQ=255)                                                                                                                                                  atacggcatgaactaattcaggttagctaaatgcttgattaaaaaggcgctactcggcatggggaagcgccttttttataggtgtcacaaaggaCATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCAAcgc                                                                                                  >  1:86882‑M2/95‑251 (MQ=255)                                                                                                                                                   tacggcatgaactaattcaggttagctaaatgcttgattaaaaaggcgctactcggcatggggaagcgccttttttataggtgtcacaaaggaCATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCAAcgcg                                                                                                 >  1:183618‑M2/94‑251 (MQ=255)                                                                                                                                                                                 tgcttgattaaaaaggcgctactcggcatggggaagcgccttttttataggtgtcacaaaggaCATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCAACGCGTGCCGACTGCACAAAAGCCGGTGCCTGTTg                                                                   >  1:215320‑M2/64‑251 (MQ=255)                                                                                                                                                                                         taaaaaggcgctactcggcatggggaagcgccttttttataggtgtcacaaaggaCATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCAACGCGTGCCGACTGCACAAAAGCCGGTGCCTGTTGGGTCTTc                                                            >  1:148682‑M2/56‑250 (MQ=255)                                                                                                                                                                                          aaaaaggcgctactcggcatggggaagcgccttttttataggtgtcacaaaggaCATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCAACGCGTGCCGACTGCACAAAAGCCGGTGCCTGTTGGGTCTTCa                                                           <  1:297010‑M2/196‑1 (MQ=255)                                                                                                                                                                                            aaaggcgctactctgcatggtgagtcgcgttttttataggtgtcacaaaggaCATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAAGCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCTCCGTTGCTGAACTTGGCATTTTTGCAGGCTAACTGGGTTGGCTCAACGCGTGCCGACTTCACAAAAGCCGGTTTCTGTTGGGTCTTCATcc                                                        <  2:411687‑M2/199‑1 (MQ=255)                                                                                                                                                                                               ggcgctactcggcatggggaagcgccttttttataggtgtcacaaaggaCATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCAACGCGTGCCGACTGCACAAAAGCCGGTGCCTGTTGGGTCTTCATCCacg                                                     <  2:176037‑M2/202‑1 (MQ=255)                                                                                                                                                                                                     attcggcatggggaagcgccgtttttataggggtcacaaaggaCATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTATCTGGGTTGGCTCAACGCGTGCCGACTGCACAAAAGCCGGTGCCTGTTGGGTCTTTATCCACGAACGAt                                               <  2:90984‑M2/208‑1 (MQ=255)                                                                                                                                                                                                            atggggaagcgacttgtttataggtgtcacanaggaCATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAAGCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCAACGCGTGCCGACTGCACAAAAGCCGGTGCCTGTTGGGTCTTCATCCACGAACGATTTGGTCa                                        <  2:235393‑M2/215‑1 (MQ=255)                                                                                                                                                                                                              ggggaagcgcctttgttataggtgtcacaaaggaCATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCAACGCGTGCCGACTGCACAAAAGCCGGTGCCTGTTGGGTCTTCATCCACGAACGATTTGGTCAg                                       <  2:360048‑M2/216‑1 (MQ=255)                                                                                                                                                                                                                      gccttttttataggtgtcacaaaggaCATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGCCTATTGGCTGCATATGGTTGAGGTGGGAACTGATCCCACCGGTGCTGAACTGGGCATTTTTGCTGGCTAACTGGGTTGGCTCAACGCGTGCCGCCTGCACAAAAGCCGGTGCCTGTTGGGTCTTCATCCacgaa                                                   <  1:288294‑M2/204‑1 (MQ=255)                                                                                                                                                                                                                      gccttttttataggtgtcacaaaggaCATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCAACGCGTGCCGACTGCACAAAAGCCGGTGCCTGTTGGGTCTTCATCCACGAACGATTTGGTCAGTTtatgtat                              <  1:117938‑M2/225‑1 (MQ=255)                                                                                                                                                                                                                      gccttttttataggggtcacaacggcCATAACTTAAGCCGCGCGATGGTGTTGGTGCGGAAAAATCTGTTCTCCAGCTGACGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGAAACTGATCCCCCCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCAACGCGCGCCGACTGCACAAAAGCCGGGGCCTGTTGGGTcttcatccacgaaagatcggactcgcgtcgtgg                               >  2:288294‑M2/27‑234 (MQ=255)                                                                                                                                                                                                                             ttataggtgtcacaaaggaCATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCAACGCGTGCCGACTGCACAAAAGCCGGTGCCTGTTGGGTCTTCATCCACGAACGATTTGGTCAGTTTATGTATGGGCtt                        <  1:159029‑M2/231‑1 (MQ=255)                                                                                                                                                                                                                                     gtcacaaaggaCATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCAACGCGTGCCGACTGCACAAAAGCCGGTGCCTGTTGGGTCTTCATCCACGAACGATTTGGTCAGTTTATGTATGGGCTTTACCcaca                <  2:300644‑M2/239‑1 (MQ=255)                                                                                                                                                                                                                                           aaggaCATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCCTTTTTGCAGGCTAACTGGGTTGGCTCAACGCGTGCCGCCTGCACAAAAGCCGGTGCCTGTTGGGTCTTCATCCACGAACGATTTGGTCAGTTTATGTATGGGCTTTACCCACACGACCa          >  1:349201‑M2/6‑250 (MQ=255)                                                                                                                                                                                                                                            aggaCATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGGTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCAACGCGTGCCGACTGCACAAAAGCCGGTGCCTGTTGGGTCTTCATCCACGAACGATTTGGTCAGTTTATGTATGGGCTTTACCCACACGACCAAc        <  1:187240‑M2/247‑1 (MQ=255)                                                                                                                                                                                                                                                   aaCTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCAACGCGTGCCGACTGCACAAAAGCCGGTGCCTGTTGGGTCTTCATCCACGAACGATTTGGTCAGTTTATGTATGGGCTTTACCCACACGACCAACGCTGGc  <  1:398948/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      ACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCAACGCGTGCCGACTGCACAAAAGCCGGTGCCTGTTGGGTCTTCATCCACGAACGATTTGGTCAGTTTATGTATGGGCTTTACCCACACGACCAACGCTGGC  >  NC_000913/3421128‑3421619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑