Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,410,666	C→G	V129V (GTC→GTG)	dusB →	tRNA‑dihydrouridine synthase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,410,666	0	C	G	100.0%	232.7 / NA	66	V129V (GTC→GTG)	dusB	tRNA‑dihydrouridine synthase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (39/27);  total (39/27)

AGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGC  >  NC_000913/3410419‑3410905                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 aGGTTTGGGAAAGCGACAAATCGCGTTTACGGATGGTGCACATTGATGAACCCGGTAGTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAAGGGCAGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGGCGTGAAt                                                                                                                                                                                                                                               <  2:36093/251‑1 (MQ=255)         gAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGa                                                                                                                                                                                                                                       <  1:74179/251‑1 (MQ=255)          aaaGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGAc                                                                                                                                                                                                                                      >  1:64553/1‑251 (MQ=255)               gACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTcc                                                                                                                                                                                                                                 >  1:126061/1‑251 (MQ=255)                        cGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTAcc                                                                                                                                                                                                                          <  1:394661/249‑1 (MQ=255)                             aCGGATGGTGCACATTGATGAACCCGGTATNCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTCCCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAg                                                                                                                                                                                                                    <  2:214637/250‑1 (MQ=255)                                          aTTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTg                                                                                                                                                                                                     >  1:291697/1‑251 (MQ=255)                                             gatgaACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAACAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGCATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGACACCggagggg                                                                                                                                                                                                   >  2:298845/1‑246 (MQ=255)                                              atgaACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCCGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTCCCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGc                                                                                                                                                                                                  >  1:436828/1‑250 (MQ=255)                                                 aaCCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCg                                                                                                                                                                                              >  1:427745/1‑251 (MQ=255)                                                  aCCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCgg                                                                                                                                                                                             <  2:30318/251‑1 (MQ=255)                                                       gTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAacac                                                                                                                                                                                        <  1:52278/251‑1 (MQ=255)                                                         aTTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGCTTCG‑CACCGGCTGGGCACCGGAACACCg                                                                                                                                                                                      >  2:354489/1‑251 (MQ=255)                                                          ttCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGt                                                                                                                                                                                     >  1:457235/1‑251 (MQ=255)                                                          ttCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGGATTAACGTGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGATCGCAGGTTCAGCCCCCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAAGTCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTTGGCACCGGAACACCGt                                                                                                                                                                                     >  2:304417/1‑251 (MQ=255)                                                             gCACCGTGCAAATTGCTGGTAGAGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAAAGCGGAGCCTAGATTATTGAGATCAATATGGGGTGCCCGGCTAAAAATGTGAATCGCAAGCTCGCAGGTTTAGCCCTCTTGCAGTACCCGGATGTCGTTTAATCGATCCTTACCGAGGTCGTGAATGCAGGGGCCGTGCCTGTTAACCTGTAACTTCG‑CACAGGCTGTGCACGGGAAAACCGTAAc                                                                                                                                                                                  >  2:74064/1‑251 (MQ=255)                                                              caacGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCTCGTATTAACGTGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGGTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACt                                                                                                                                                                                 <  2:447075/248‑1 (MQ=255)                                                                   tGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTTG‑CACCGGCTGGGCCCCGGCACACCGTAACTGCGa                                                                                                                                                                             >  2:274393/1‑250 (MQ=255)                                                                    gCAAATTGCTGGTAGGGATCCGAAAGAAAGGGCAGATGCAGCAAGTATTAACGGGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCACGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGGTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACGCCGTAACTGCGAag                                                                                                                                                                           <  2:189409/251‑1 (MQ=255)                                                                          tGCTGGTAGCTATCCGAAAGAAATGTTATATGCAGCACGTATGAACGTGGAAAGCGGTGCCCAGATTATTGATATCAATAAGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGTATGTCGTTAAATCGATCCTTACCGAGTGCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGAGTCG‑CACCGGCTGGGCACCGGAACTCAGTAACTGCGAAGAGAtt                                                                                                                                                                      <  2:77873/250‑1 (MQ=255)                                                                           gagggTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAAAGCGGTGACCAGAGTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCAACGGAACACCGTAACTGAGAAGAGATTg                                                                                                                                                                     <  2:337352/247‑1 (MQ=255)                                                                               gTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAAATGCGAAGAGATTGCCCa                                                                                                                                                                 >  1:105621/1‑250 (MQ=255)                                                                                     aTCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCt                                                                                                                                                          <  1:411919/251‑1 (MQ=255)                                                                                       cctaaagaagTGGCAGATGCAGCACGTATTAAAGTGGAAATCGGTGCCCAGATTATTGATATCAATATGGGATGCCCGGCTAAAACAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGAACCCGGATGTCGTTACATCGCTCCTTAACGAGGTCGTGAATGCAGCGGACGTTCCTGTTACACTGAAAATCCG‑CACCCGCTGGGCAACGGCACCCCGTAACTCAGAAGAGATTTACCAAATGGCTg                                                                                                                                                         >  2:195217/4‑250 (MQ=255)                                                                                       ccGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAATAGATTGCCCATCGGGCTg                                                                                                                                                         <  2:402829/250‑1 (MQ=255)                                                                                       cagaaagaaaTGGCAGATGCAGCACGTATTAAAGTGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCACTCTTGCCGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTAGTGAATGAAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCCACGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTgt                                                                                                                                                        >  2:352640/3‑250 (MQ=255)                                                                                             gaaaTGGCAGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACtg                                                                                                                                                  >  1:426489/1‑251 (MQ=255)                                                                                                     aGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTc                                                                                                                                          <  1:2391/251‑1 (MQ=255)                                                                                                       aTGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTc                                                                                                                                          >  1:66339/1‑249 (MQ=255)                                                                                                                gTATTAACGTGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGAcc                                                                                                                               <  1:63731/251‑1 (MQ=255)                                                                                                                         tGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGc                                                                                                                      >  2:11458/1‑251 (MQ=255)                                                                                                                             aaGCGGTGCCCAGCTTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAAATGCCTGAAGACTGTGGCATTCAGGCCCAGACCATTCATAGCCGtc                                                                                                                  >  2:307096/1‑250 (MQ=255)                                                                                                                                cGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTacac                                                                                                               <  1:432506/251‑1 (MQ=255)                                                                                                                                   tGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACAcgcg                                                                                                            >  1:466086/1‑251 (MQ=255)                                                                                                                                    gCCCAGTTTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGCTTCG‑CACCGGCGGGGCCCCGGAACACCGTCACTGCGAAGAGATTGCCCAAATGGCTGAAGACTGTGGCATTCAGGCTCTGCCCATTCATGGCCGTACACgcgc                                                                                                           >  2:168623/1‑251 (MQ=255)                                                                                                                                        aaagtgttGATATCGGTATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTTGAAGGTTCAGCCCGCTTGCAGTACCAGGATGTTGTTAAGTCGATCCTTACCGAGGTCGCGAAGGCGATGGACGATCTTGGTACCCTGTAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGACGAGATTGCCCAACTGGCTGGCGACTGTTGCATTCCGGNTAGGACAATTAATGGCCATAAACGCGCCtgt                                                                                                       >  2:224350/7‑251 (MQ=255)                                                                                                                                            tattGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTAGCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGCCGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGAGTGTGGCATTCAGGCTCTNACCATTCATGGCCGTACACGCGCCtgtttgt                                                                                                   >  2:229680/1‑251 (MQ=255)                                                                                                                                             attGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCtgtttgtt                                                                                                  >  1:348156/1‑251 (MQ=255)                                                                                                                                                gATAGCAATATGGGTTGCCCGGCTAAAAAAGGGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACTCCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCaa                                                                                               <  2:427745/251‑1 (MQ=255)                                                                                                                                                 atatCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGAAGTTCCTGTTACCCTGAAGATTCG‑CAACGGCTGGGAACCGGAACACAGTAACTGCGAAGAGATTGCCCAACTGGCTGATGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACgcgcattgttgttaaat                                                                                              >  2:91595/1‑238 (MQ=255)                                                                                                                                                  tatCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATg                                                                                             >  1:8046/1‑251 (MQ=255)                                                                                                                                                           gggTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTg                                                                                    <  1:50440/251‑1 (MQ=255)                                                                                                                                                                 cccGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACg                                                                              <  2:126061/251‑1 (MQ=255)                                                                                                                                                                    gggtAAAAAAGTGAATCGCAAGCTCGCAGGTGCAGCCCTCTTGCAGTACCCGGATGTCGTTAAAACGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATGCG‑CACCGGCTGGGCACCGTAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGata                                                                           <  2:53042/248‑3 (MQ=255)                                                                                                                                                                                gAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTCCCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCCCCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCat                                                               <  2:320377/251‑2 (MQ=255)                                                                                                                                                                                         gCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGCTCCTTACCGAGGTCGTGCATGCAGAGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGCCCACCGTAACTGCGCAGAGATTGACCAACTGGCTGAAGACTGAGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGCGTCCGCAAGAATTCGGGCAGTTAAGc                                                         >  2:431483/1‑248 (MQ=255)                                                                                                                                                                                            cGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCCATGGCGAAGCTGANTACGACAGGATTCGGGCAGTTAAGCAGaact                                                   >  2:227617/1‑249 (MQ=255)                                                                                                                                                                                             gCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGt                                                  >  1:46021/1‑251 (MQ=255)                                                                                                                                                                                              cAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGtt                                                 <  1:397305/251‑1 (MQ=255)                                                                                                                                                                                                  ttCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTcc                                              >  1:296420/1‑250 (MQ=255)                                                                                                                                                                                                         ctctTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGt                                      >  2:47933/1‑251 (MQ=255)                                                                                                                                                                                                         ctctTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGt                                      >  1:273794/1‑251 (MQ=255)                                                                                                                                                                                                             tGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGtt                                     <  1:424368/248‑1 (MQ=255)                                                                                                                                                                                                              gCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATcg                                 >  1:250232/1‑251 (MQ=255)                                                                                                                                                                                                                   ccccGGATGTCGTTAAAGCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTGCCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAAt                            <  2:64553/250‑1 (MQ=255)                                                                                                                                                                                                                        gATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTTCGACAGTATTGGGGCAGTTAAGCAGAAGGTTTCCATTCCGGTTATCGCGATTGGCg                        >  2:372971/1‑250 (MQ=255)                                                                                                                                                                                                                          tGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGAGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGAcc                     >  1:188746/1‑250 (MQ=255)                                                                                                                                                                                                                            tCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGCCAATATTCGGGCAGTTACGCAGAAAGTTTCCATTCCGGTTATCGAGAATGGCGACAtt                   >  2:391994/1‑251 (MQ=255)                                                                                                                                                                                                                                  aaTCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGAc             <  1:209109/251‑1 (MQ=255)                                                                                                                                                                                                                                       aTCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATGACTGACCCGCt        <  1:354489/251‑1 (MQ=255)                                                                                                                                                                                                                                         ccTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAAGGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATAACTGACACGCtt       >  2:17280/1‑250 (MQ=255)                                                                                                                                                                                                                                            tACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTaa     >  1:167770/1‑249 (MQ=255)                                                                                                                                                                                                                                            tACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTaa     <  1:274393/249‑1 (MQ=255)                                                                                                                                                                                                                                            tACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTaa     >  1:473653/1‑249 (MQ=255)                                                                                                                                                                                                                                             aCCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAg   <  1:11458/250‑1 (MQ=255)                                                                                                                                                                                                                                             aCCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACCGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGCATGGCGACATTACTGACCCGCTTAAAGc  >  2:343388/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 AGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCG‑CACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGC  >  NC_000913/3410419‑3410905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑